Marija Zanic, Ph.D.

Assistant Professor

marija.zanic@vanderbilt.edu

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Faculty Appointments
Assistant Professor of Cell and Developmental Biology Assistant Professor of Chemical and Biomolecular Engineering
Education
Ph.D., Physics, University of Texas, Austin, TexasM.S., Physics, University of Zagreb, Zagreb, Croatia (Hrvatska)
Office Address
465 21st Avenue South
4120 MRBIII
Nashville, TN 37240
Research Keywords
molecular cell biology, physical biology, biophysics, cytoskeleton, microtubules, microtubule dynamics
Publications
Zanic M. Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro. Methods Mol. Biol. 2016; 1413: 47-61. PMID: 27193842, DOI: 10.1007/978-1-4939-3542-0_4, ISSN: 1940-6029.

Ohi R, Zanic M. Ahead of the Curve: New Insights into Microtubule Dynamics. F1000Res. 2016; 5: PMID: 26998244, PMCID: PMC4786905, DOI: 10.12688/f1000research.7439.1.

Grimaldi AD, Zanic M, Kaverina I. Encoding the microtubule structure: Allosteric interactions between the microtubule +TIP complex master regulators and TOG-domain proteins. Cell Cycle. 2015; 14(9): 1375-8. PMID: 25895033, PMCID: PMC4614873, DOI: 10.1080/15384101.2015.1026521, ISSN: 1551-4005.

Zanic M, Widlund PO, Hyman AA, Howard J. Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels. Nat. Cell Biol [print-electronic]. 2013 Jun; 15(6): 688-93. PMID: 23666085, PII: ncb2744, DOI: 10.1038/ncb2744, ISSN: 1476-4679.

Bowne-Anderson H, Zanic M, Kauer M, Howard J. Microtubule dynamic instability: a new model with coupled GTP hydrolysis and multistep catastrophe. Bioessays [print-electronic]. 2013 May; 35(5): 452-61. PMID: 23532586, PMCID: PMC3677417, DOI: 10.1002/bies.201200131, ISSN: 1521-1878.

Gardner MK, Zanic M, Howard J. Microtubule catastrophe and rescue. Curr. Opin. Cell Biol [print-electronic]. 2013 Feb; 25(1): 14-22. PMID: 23092753, PMCID: PMC3556214, PII: S0955-0674(12)00145-7, DOI: 10.1016/j.ceb.2012.09.006, ISSN: 1879-0410.

Tropini C, Roth EA, Zanic M, Gardner MK, Howard J. Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues. PLoS ONE [print-electronic]. 2012; 7(1): e30103. PMID: 22272281, PMCID: PMC3260198, PII: PONE-D-11-21334, DOI: 10.1371/journal.pone.0030103, ISSN: 1932-6203.

Gardner MK, Zanic M, Gell C, Bormuth V, Howard J. Depolymerizing kinesins Kip3 and MCAK shape cellular microtubule architecture by differential control of catastrophe. Cell. 2011 Nov 11/23/2011; 147(5): 1092-103. PMID: 22118464, PII: S0092-8674(11)01287-6, DOI: 10.1016/j.cell.2011.10.037, ISSN: 1097-4172.

Widlund PO, Stear JH, Pozniakovsky A, Zanic M, Reber S, Brouhard GJ, Hyman AA, Howard J. XMAP215 polymerase activity is built by combining multiple tubulin-binding TOG domains and a basic lattice-binding region. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2011 Feb 2/15/2011; 108(7): 2741-6. PMID: 21282620, PMCID: PMC3041093, PII: 1016498108, DOI: 10.1073/pnas.1016498108, ISSN: 1091-6490.

Gell C, Bormuth V, Brouhard GJ, Cohen DN, Diez S, Friel CT, Helenius J, Nitzsche B, Petzold H, Ribbe J, Schäffer E, Stear JH, Trushko A, Varga V, Widlund PO, Zanic M, Howard J. Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy. Methods Cell Biol. 2010; 95: 221-45. PMID: 20466138, PII: S0091-679X(10)95013-9, DOI: 10.1016/S0091-679X(10)95013-9, ISSN: 0091-679X.

Zanic M, Stear JH, Hyman AA, Howard J. EB1 recognizes the nucleotide state of tubulin in the microtubule lattice. PLoS ONE. 2009; 4(10): e7585. PMID: 19851462, PMCID: PMC2761489, DOI: 10.1371/journal.pone.0007585, ISSN: 1932-6203.

Lee YC, Block G, Chen H, Folch-Puy E, Foronjy R, Jalili R, Jendresen CB, Kimura M, Kraft E, Lindemose S, Lu J, McLain T, Nutt L, Ramon-Garcia S, Smith J, Spivak A, Wang ML, Zanic M, Lin SH. One-step isolation of plasma membrane proteins using magnetic beads with immobilized concanavalin A. Protein Expr. Purif [print-electronic]. 2008 Dec; 62(2): 223-9. PMID: 18765283, PMCID: PMC2600885, PII: S1046-5928(08)00215-5, DOI: 10.1016/j.pep.2008.08.003, ISSN: 1096-0279.

Available Postdoctoral Position Details
Posted: 12/5/2014
The Zanic Lab has current openings for outstanding postdoctoral researchers interested in cytoskeletal dynamics and organization. Our lab seeks to understand the molecular mechanisms underlying microtubule dynamic instability and its regulation. Our approaches combine biochemical in vitro reconstitution with purified protein components, single molecule TIRF microscopy, quantitative image analysis and theoretical modeling. We are looking for motivated, enthusiastic and talented researchers from a wide variety of backgrounds. Experience with any of the following is beneficial: protein expression and purification, microfluidics, image analysis/Matlab. To apply, please contact Marija Zanic at marija.zanic@vanderbilt.edu