Gregor Neuert, Ph.D.Assistant Professor
A hallmark of life is organisms’ ability to sense and respond to internal and external signals. Our research interests focus on quantitative and predictive understanding of dynamics in signal transduction and regulation of the coding and the noncoding genome related to human health. We are using a cross-disciplinary approach of biology, physics, engineering and computer science by combining quantitative single-molecule experiments in individual cells with genetics and computational biology. Results from this quantitative systems biology research will help to answer key questions in signal transduction and gene regulation of healthy and diseased tissue, and will significantly deepen our understanding of fundamental biophysical and molecular principles in various research fields.
Current research areas are:
1. To develop physiologically relevant cell perturbations to understand biological systems.
2. To understand the function of the noncoding genome.
3. To revolutionize model predictions in human health.
Munsky B, Fox Z, Neuert G. Integrating single-molecule experiments and discrete stochastic models to understand heterogeneous gene transcription dynamics. Methods [print-electronic]. 2015 Sep 9/1/2015; 85: 12-21. PMID: 26079925, PMCID: PMC4537808, PII: S1046-2023(15)00251-0, DOI: 10.1016/j.ymeth.2015.06.009, ISSN: 1095-9130.
Munsky B, Neuert G. From analog to digital models of gene regulation. Phys Biol. 2015 Jul; 12(4): 45004. PMID: 26086470, PMCID: PMC4591055, DOI: 10.1088/1478-3975/12/4/045004, ISSN: 1478-3975.
Neuert G, Munsky B, Tan RZ, Teytelman L, Khammash M, van Oudenaarden A. Systematic identification of signal-activated stochastic gene regulation. Science. 2013 Feb 2/1/2013; 339(6119): 584-7. PMID: 23372015, PMCID: PMC3751578, PII: 339/6119/584, DOI: 10.1126/science.1231456, ISSN: 1095-9203.
van Werven FJ, Neuert G, Hendrick N, Lardenois A, Buratowski S, van Oudenaarden A, Primig M, Amon A. Transcription of two long noncoding RNAs mediates mating-type control of gametogenesis in budding yeast. Cell [print-electronic]. 2012 Sep 9/14/2012; 150(6): 1170-81. PMID: 22959267, PMCID: PMC3472370, PII: S0092-8674(12)00939-7, DOI: 10.1016/j.cell.2012.06.049, ISSN: 1097-4172.
Munsky B, Neuert G, van Oudenaarden A. Using gene expression noise to understand gene regulation. Science. 2012 Apr 4/13/2012; 336(6078): 183-7. PMID: 22499939, PMCID: PMC3358231, PII: 336/6078/183, DOI: 10.1126/science.1216379, ISSN: 1095-9203.
Bumgarner SL, Neuert G, Voight BF, Symbor-Nagrabska A, Grisafi P, van Oudenaarden A, Fink GR. Single-cell analysis reveals that noncoding RNAs contribute to clonal heterogeneity by modulating transcription factor recruitment. Mol. Cell [print-electronic]. 2012 Feb 2/24/2012; 45(4): 470-82. PMID: 22264825, PMCID: PMC3288511, PII: S1097-2765(11)00996-8, DOI: 10.1016/j.molcel.2011.11.029, ISSN: 1097-4164.
Zimmermann JL, Nicolaus T, Neuert G, Blank K. Thiol-based, site-specific and covalent immobilization of biomolecules for single-molecule experiments. Nat Protoc. 2010 Jun; 5(6): 975-85. PMID: 20448543, PII: nprot.2010.49, DOI: 10.1038/nprot.2010.49, ISSN: 1750-2799.
Albrecht CH, Neuert G, Lugmaier RA, Gaub HE. Molecular force balance measurements reveal that double-stranded DNA unbinds under force in rate-dependent pathways. Biophys. J [print-electronic]. 2008 Jun; 94(12): 4766-74. PMID: 18339733, PMCID: PMC2397355, PII: S0006-3495(08)70343-6, DOI: 10.1529/biophysj.107.125427, ISSN: 1542-0086.
Neuert G, Albrecht CH, Gaub HE. Predicting the rupture probabilities of molecular bonds in series. Biophys. J [print-electronic]. 2007 Aug 8/15/2007; 93(4): 1215-23. PMID: 17468164, PMCID: PMC1929050, PII: S0006-3495(07)71379-6, DOI: 10.1529/biophysj.106.100511, ISSN: 0006-3495.
Neuert G, Albrecht C, Pamir E, Gaub HE. Dynamic force spectroscopy of the digoxigenin-antibody complex. FEBS Lett [print-electronic]. 2006 Jan 1/23/2006; 580(2): 505-9. PMID: 16388805, PII: S0014-5793(05)01535-8, DOI: 10.1016/j.febslet.2005.12.052, ISSN: 0014-5793.
Postdoctoral Fellow Position in Quantitative Single Cell Biology
A Postdoctoral Fellow position is immediately available for a highly motivated upcoming or recent Ph.D. or M.D./Ph.D. graduate in Mammalian Cell Biology or related biological field in the lab of Dr. Gregor Neuert, NIH Director’s New Innovator Awardee 2014, at Vanderbilt University, School of Medicine in Nashville, Tennessee.
The Neuert lab works on the leading edge in the quantitative understanding of molecular mechanism contributing to the function and malfunction of signal transduction and gene regulatory processes in yeast and mammalian cells. Our research methods include a combination of single-molecule / single-cell experiments, next generation sequencing, predictive computational modeling, molecular biology and genetics.
We are seeking highly motivated, driven and curious applicants (recent graduate with no more than 1 year of previous postdoctoral training), a rich set of skills in mammalian cell culture and mammalian cell biology techniques with a focus on signaling or transcription, a strong background, proven record (first author publications) and rigorous training in one of the following areas: stem cell biology, cancer biology, immunology, pharmacology, developmental biology, or neuroscience.
The ideal candidate will combine his/her cell biological training with quantitative approaches from the Neuert lab to ask fundamental question related to how environmental changes over time impact cellular phenotypes, cell signaling and gene expression in single cells (see Li, Neuert, bioRxiv, 2017). The ideal candidate should have some basic quantitative training or is expected to learn computer programing.
We offer training in single cell biology, quantitative biology, computer programing, complex data analysis, novel experimental design, in addition to an exciting, highly interactive, international, interdisciplinary, and well-funded research environment with strong mentoring and career development support.
Vanderbilt University School of Medicine is ranked #8 in NIH funding, #14 in U.S. News Medical School Ranking, #25 in U.S. News Biomedical Engineering Ranking, #3 in Physiology and is equipped with world-class cutting-edge experimental and computational core facilities. Nashville is a mecca for high quality music, great restaurants, rapid access to outdoor activities and is centrally located within the US.
Interested applicants should send a curriculum vita, a summary of research experience and accomplishments, and contact information of 2-3 references to firstname.lastname@example.org. Applicants should explain briefly why they are interested in pursuing a postdoctoral position in general and why in the Neuert lab.
For more information please visit: https://medschool.vanderbilt.edu/neuert-lab