Gregor Neuert, Ph.D.

Assistant Professor

gregor.neuert@vanderbilt.edu

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Faculty Appointments
Assistant Professor of Molecular Physiology and Biophysics Assistant Professor of PharmacologyAssistant Professor of Biomedical Engineering
Education
Ph.D., Physics, Ludwig-Maximilians-Universit├Ąt , Munich, GermanyM.Eng., Ilmenau University of Technology, Ilmenau, Germany
Office Address
813C light Hall
2215 Garland Avenue
Nashville, TN 37232-0615
Research Description
A current molecular medicine topic of interest is how cells respond dynamically to changes in their environment utilizing their cellular gene, RNA and protein networks. We aim to approach this question by investigating endogenous signal transduction and transcriptional regulatory networks of coding and non-coding RNA in yeast and mammalian cells. Yeast is an ideal model organism for studying these questions because it can be easily manipulated genetically and many biological principles are conserved in humans. The regulatory principles discovered in yeast will then be tested in healthy and diseased mammalian tissue to test their generality. We aim to investigate the architecture and functioning of these networks by measuring the dynamics of protein and RNA levels in single cells. Our research methods include a combination of single-cell techniques such as flow cytometry, live cell time-lapse microscopy, fluorescent in-situ hybridization with single-molecule resolution at the RNA level (single-molecule RNA-FISH) in cells and tissue samples as well as single-molecule-based modeling. The main advantage to quantify single cells is to distinguish between different regulatory mechanisms, which cannot be observed in population-based experiments. Since our approach is general, it can lead to similar quantitative understanding of many genes, pathways or organisms ranging from yeast to human.
Research Keywords
Quantitative Systems Biology of signal transduction and gene regulation of coding and non-coding RNA
Publications
Munsky B, Fox Z, Neuert G. Integrating single-molecule experiments and discrete stochastic models to understand heterogeneous gene transcription dynamics. Methods [print-electronic]. 2015 Sep 9/1/2015; 85: 12-21. PMID: 26079925, PMCID: PMC4537808, PII: S1046-2023(15)00251-0, DOI: 10.1016/j.ymeth.2015.06.009, ISSN: 1095-9130.

Munsky B, Neuert G. From analog to digital models of gene regulation. Phys Biol. 2015 Jul; 12(4): 45004. PMID: 26086470, PMCID: PMC4591055, DOI: 10.1088/1478-3975/12/4/045004, ISSN: 1478-3975.

Neuert G, Munsky B, Tan RZ, Teytelman L, Khammash M, van Oudenaarden A. Systematic identification of signal-activated stochastic gene regulation. Science. 2013 Feb 2/1/2013; 339(6119): 584-7. PMID: 23372015, PMCID: PMC3751578, PII: 339/6119/584, DOI: 10.1126/science.1231456, ISSN: 1095-9203.

van Werven FJ, Neuert G, Hendrick N, Lardenois A, Buratowski S, van Oudenaarden A, Primig M, Amon A. Transcription of two long noncoding RNAs mediates mating-type control of gametogenesis in budding yeast. Cell [print-electronic]. 2012 Sep 9/14/2012; 150(6): 1170-81. PMID: 22959267, PMCID: PMC3472370, PII: S0092-8674(12)00939-7, DOI: 10.1016/j.cell.2012.06.049, ISSN: 1097-4172.

Munsky B, Neuert G, van Oudenaarden A. Using gene expression noise to understand gene regulation. Science. 2012 Apr 4/13/2012; 336(6078): 183-7. PMID: 22499939, PMCID: PMC3358231, PII: 336/6078/183, DOI: 10.1126/science.1216379, ISSN: 1095-9203.

Bumgarner SL, Neuert G, Voight BF, Symbor-Nagrabska A, Grisafi P, van Oudenaarden A, Fink GR. Single-cell analysis reveals that noncoding RNAs contribute to clonal heterogeneity by modulating transcription factor recruitment. Mol. Cell [print-electronic]. 2012 Feb 2/24/2012; 45(4): 470-82. PMID: 22264825, PMCID: PMC3288511, PII: S1097-2765(11)00996-8, DOI: 10.1016/j.molcel.2011.11.029, ISSN: 1097-4164.

Zimmermann JL, Nicolaus T, Neuert G, Blank K. Thiol-based, site-specific and covalent immobilization of biomolecules for single-molecule experiments. Nat Protoc. 2010 Jun; 5(6): 975-85. PMID: 20448543, PII: nprot.2010.49, DOI: 10.1038/nprot.2010.49, ISSN: 1750-2799.

Albrecht CH, Neuert G, Lugmaier RA, Gaub HE. Molecular force balance measurements reveal that double-stranded DNA unbinds under force in rate-dependent pathways. Biophys. J [print-electronic]. 2008 Jun; 94(12): 4766-74. PMID: 18339733, PMCID: PMC2397355, PII: S0006-3495(08)70343-6, DOI: 10.1529/biophysj.107.125427, ISSN: 1542-0086.

Neuert G, Albrecht CH, Gaub HE. Predicting the rupture probabilities of molecular bonds in series. Biophys. J [print-electronic]. 2007 Aug 8/15/2007; 93(4): 1215-23. PMID: 17468164, PMCID: PMC1929050, PII: S0006-3495(07)71379-6, DOI: 10.1529/biophysj.106.100511, ISSN: 0006-3495.

Neuert G, Albrecht C, Pamir E, Gaub HE. Dynamic force spectroscopy of the digoxigenin-antibody complex. FEBS Lett [print-electronic]. 2006 Jan 1/23/2006; 580(2): 505-9. PMID: 16388805, PII: S0014-5793(05)01535-8, DOI: 10.1016/j.febslet.2005.12.052, ISSN: 0014-5793.

Available Postdoctoral Position Details
Posted: 7/2/2015
We are seeking highly motivated, driven and curious recent graduates (not more then 2 years of postdoctoral training) with programing experience in Matlab, Python or R and with a strong background, proven record (first author publications) and rigorous training in one or more of the following areas: biophysics, cancer biology, computational biology, computational modeling, control and dynamical systems theory, immunology, neuroscience, pharmacology, single molecule biophysics, systems biology or yeast genetics.

Please send an email with your CV, contact addresses of three references and a short description why you are interested in joining the lab. Applicants are encouraged to apply for individual postdoctoral fellowships.