Eduard Y. Chekmenev, Ph.D.

Associate Professor

eduard.chekmenev@vanderbilt.edu

Visit Lab Site


Faculty Appointments
Associate Professor of Radiology and Radiological Sciences Assistant Professor of Biomedical Engineering
Education
Ph.D., PHD - CHEMISTRY, University of Louisville, Louisville, KentuckyB.S., Perm State University, Perm, Russia
Office Address
1161 21st Avenue South
Medical Center North, AA-1105
Nashville, TN 37232-2310
Research Description
The long term goal of our research is to answer the questions about cancer metabolic disorders at molecular, cellular and tissue level and its correlation with genetic disorders and pathology. We focus on development of the predictors (often termed biomarkers) about the patient outcome and response to therapy as early as several minutes after drug administration using real time metabolic imaging. Funded projects (by NIH/NCI and Prevent Cancer Foundation) in our laboratory are conducted in cellular and rodent models of human cancer utilizing Magnetic Resonance Imaging (MRI) and its variant Magnetic Resonance Spectroscopic Imaing (MRSI) modalities. We use MR hyperpolared 13C and 15N labeled metabolic contrast agents to achieve unprecedented spatial resolution and high contrast. The advantage of the hyperpolarization techniques is the increase in MR sensitivity by 10,000-1,000,000 fold, which overcomes previous sensitivity limitations of MRI. Our laboratory at VUIIS currently utilizes parahydrogen gas and commercially availabe hyperpolarized 129Xe gas to hyperpolarize 13C and 15N contrast agents. These contrast agents are non-radioactive and use no ionizing radiation during imaging and enable a new generation of ultrasensitive, ultrafast MR imaging techniques that will be optimized for use in oncology. The persistence of polarization through chemical reactions of biochemical pathways allows sub-second MRI and MRSI examinations in real time whereas current standard of care in oncology PET-CT exam requires long examiation time and expose patients to ionizing radiation. We also hope to address the central issues, necessary for successful introduction of Clinical Trials of non-invasive and non-radioactive hyperpolarized MRSI using injectacble hyperpolarized choline, glutamate, glutamine and succinate and others as in vivo contrast imaging reagents. These biomarkers potentially allow direct imaging of real time metabolic activity of choline kinase (ChoK), succinate dehydrogenase (SDH), etc. as well as indirect imaging of hypoxia inducing factor HIF-1α and other oncogenes. We also work on receptor imaging using hyperpolarized MR, which can be useful for in vivo cancer research as well as for in vitro structural and functional studies of proteins and especially membrane associated proteins. We would like to demonstrate efficacy of hyperpolarized biomarkers for early detection of cancer and response to treatment using sub-second MRI and ultrafast MRSI and demonstrate the advantages of hyperpolarized metabolic tracers in defining tumor growth, heterogeneity and prediction of a positive response to therapy, when compared to conventional MRI and PET-CT.

Ultrafast hyperpolarized MRSI will have far-reaching impact on all areas of oncology in which current imaging technologies are insufficiently precise or insensitive to early diagnosis. We hope not only investigate underlying metabolic events of cancer with real-time metabolic imaging in laboratory setting, but also provide US population with fast, safe low-cost metabolic MR exams in the future that will be useful for population screening and treatment follow-up and would replace or augment ionizing mammography screening and expensive radioactive PET-CT.
Research Keywords
Instrumentation, chemistry and spin physics of hyperpolarized metabolic MRI tracers in biomedical research. Real time metabolic imaging of breast cancer using hyperpolarized MRI tracers.
Publications
Abramson RG, Arlinghaus LR, Dula AN, Quarles CC, Stokes AM, Weis JA, Whisenant JG, Chekmenev EY, Zhukov I, Williams JM, Yankeelov TE. MR Imaging Biomarkers in Oncology Clinical Trials. Magn Reson Imaging Clin N Am. 2016 Feb; 24(1): 11-29. PMID: 26613873, PMCID: PMC4667554, PII: S1064-9689(15)00100-2, DOI: 10.1016/j.mric.2015.08.002, ISSN: 1557-9786.

Miller CO, Cao J, Chekmenev EY, Damon BM, Cherrington AD, Gore JC. Noninvasive measurements of glycogen in perfused mouse livers using chemical exchange saturation transfer NMR and comparison to (13)C NMR spectroscopy. Anal. Chem [print-electronic]. 2015 Jun 6/2/2015; 87(11): 5824-30. PMID: 25946616, DOI: 10.1021/acs.analchem.5b01296, ISSN: 1520-6882.

Truong ML, Theis T, Coffey AM, Shchepin RV, Waddell KW, Shi F, Goodson BM, Warren WS, Chekmenev EY. (15)N Hyperpolarization by Reversible Exchange Using SABRE-SHEATH. J Phys Chem C Nanomater Interfaces [print-electronic]. 2015 Apr 4/23/2015; 119(16): 8786-97. PMID: 25960823, PMCID: PMC4419867, DOI: 10.1021/acs.jpcc.5b01799, ISSN: 1932-7447.

Shi F, Coffey AM, Waddell KW, Chekmenev EY, Goodson BM. Nanoscale Catalysts for NMR Signal Enhancement by Reversible Exchange. J Phys Chem C Nanomater Interfaces. 2015 Apr 4/2/2015; 119(13): 7525-33. PMID: 26185545, PMCID: PMC4501382, DOI: 10.1021/acs.jpcc.5b02036, ISSN: 1932-7447.

Nikolaou P, Goodson BM, Chekmenev EY. NMR hyperpolarization techniques for biomedicine. Chemistry [print-electronic]. 2015 Feb 2/16/2015; 21(8): 3156-66. PMID: 25470566, PMCID: PMC4418426, DOI: 10.1002/chem.201405253, ISSN: 1521-3765.

Kovtunov KV, Truong ML, Barskiy DA, Salnikov OG, Bukhtiyarov VI, Coffey AM, Waddell KW, Koptyug IV, Chekmenev EY. Propane-d6 Heterogeneously Hyperpolarized by Parahydrogen. J Phys Chem C Nanomater Interfaces [print-electronic]. 2014 Dec 12/4/2014; 118(48): 28234-43. PMID: 25506406, PMCID: PMC4259496, DOI: 10.1021/jp508719n, ISSN: 1932-7447.

Truong ML, Shi F, He P, Yuan B, Plunkett KN, Coffey AM, Shchepin RV, Barskiy DA, Kovtunov KV, Koptyug IV, Waddell KW, Goodson BM, Chekmenev EY. Irreversible catalyst activation enables hyperpolarization and water solubility for NMR signal amplification by reversible exchange. J Phys Chem B [print-electronic]. 2014 Dec 12/4/2014; 118(48): 13882-9. PMID: 25372972, PMCID: PMC4259498, DOI: 10.1021/jp510825b, ISSN: 1520-5207.

Theis T, Truong M, Coffey AM, Chekmenev EY, Warren WS. LIGHT-SABRE enables efficient in-magnet catalytic hyperpolarization. J. Magn. Reson [print-electronic]. 2014 Nov; 248: 23-6. PMID: 25299767, PII: S1090-7807(14)00242-0, DOI: 10.1016/j.jmr.2014.09.005, ISSN: 1096-0856.

Truong ML, Harrington MG, Schepkin VD, Chekmenev EY. Sodium 3D COncentration MApping (COMA 3D) using (23)Na and proton MRI. J. Magn. Reson [print-electronic]. 2014 Oct; 247: 88-95. PMID: 25261742, PMCID: PMC4198170, PII: S1090-7807(14)00237-7, DOI: 10.1016/j.jmr.2014.08.017, ISSN: 1096-0856.

Coffey AM, Kovtunov KV, Barskiy DA, Koptyug IV, Shchepin RV, Waddell KW, He P, Groome KA, Best QA, Shi F, Goodson BM, Chekmenev EY. High-resolution low-field molecular magnetic resonance imaging of hyperpolarized liquids. Anal. Chem [print-electronic]. 2014 Sep 9/16/2014; 86(18): 9042-9. PMID: 25162371, PMCID: PMC4165454, DOI: 10.1021/ac501638p, ISSN: 1520-6882.

Kovtunov KV, Barskiy DA, Coffey AM, Truong ML, Salnikov OG, Khudorozhkov AK, Inozemtseva EA, Prosvirin IP, Bukhtiyarov VI, Waddell KW, Chekmenev EY, Koptyug IV. High-resolution 3D proton MRI of hyperpolarized gas enabled by parahydrogen and Rh/TiO2 heterogeneous catalyst. Chemistry [print-electronic]. 2014 Sep 9/8/2014; 20(37): 11636-9. PMID: 24961814, PMCID: PMC4288780, DOI: 10.1002/chem.201403604, ISSN: 1521-3765.

Truong ML, Coffey AM, Shchepin RV, Waddell KW, Chekmenev EY. Sub-second proton imaging of 13C hyperpolarized contrast agents in water. Contrast Media Mol Imaging [print-electronic]. 2014 Sep; 9(5): 333-41. PMID: 24753438, PMCID: PMC4198480, DOI: 10.1002/cmmi.1579, ISSN: 1555-4317.

Nikolaou P, Coffey AM, Barlow MJ, Rosen MS, Goodson BM, Chekmenev EY. Temperature-ramped (129)Xe spin-exchange optical pumping. Anal. Chem [print-electronic]. 2014 Aug 8/19/2014; 86(16): 8206-12. PMID: 25008290, PMCID: PMC4139178, DOI: 10.1021/ac501537w, ISSN: 1520-6882.

Zu Z, Xu J, Li H, Chekmenev EY, Quarles CC, Does MD, Gore JC, Gochberg DF. Imaging amide proton transfer and nuclear overhauser enhancement using chemical exchange rotation transfer (CERT). Magn Reson Med [print-electronic]. 2014 Aug; 72(2): 471-6. PMID: 24302497, PMCID: PMC4401079, DOI: 10.1002/mrm.24953, ISSN: 1522-2594.

Shchepin RV, Chekmenev EY. Toward hyperpolarized molecular imaging of HIV: synthesis and longitudinal relaxation properties of (15) N-Azidothymidine. J Labelled Comp Radiopharm [print-electronic]. 2014 Aug; 57(10): 621-4. PMID: 25156931, PMCID: PMC4287256, DOI: 10.1002/jlcr.3220, ISSN: 1099-1344.

Shi F, Coffey AM, Waddell KW, Chekmenev EY, Goodson BM. Heterogeneous solution NMR signal amplification by reversible exchange. Angew. Chem. Int. Ed. Engl [print-electronic]. 2014 Jul 7/14/2014; 53(29): 7495-8. PMID: 24889730, DOI: 10.1002/anie.201403135, ISSN: 1521-3773.

Kovtunov KV, Barskiy DA, Shchepin RV, Coffey AM, Waddell KW, Koptyug IV, Chekmenev EY. Demonstration of heterogeneous parahydrogen induced polarization using hyperpolarized agent migration from dissolved Rh(I) complex to gas phase [letter]. Anal. Chem [print-electronic]. 2014 Jul 7/1/2014; 86(13): 6192-6. PMID: 24918975, PMCID: PMC4079322, DOI: 10.1021/ac5013859, ISSN: 1520-6882.

Shchepin RV, Pham W, Chekmenev EY. Dephosphorylation and biodistribution of 1-¹³C-phospholactate in vivo. J Labelled Comp Radiopharm [print-electronic]. 2014 Jun 6/30/2014; 57(8): 517-24. PMID: 24995802, PMCID: PMC4287379, DOI: 10.1002/jlcr.3207, ISSN: 1099-1344.

Shchepin RV, Coffey AM, Waddell KW, Chekmenev EY. Parahydrogen induced polarization of 1-(13)C-phospholactate-d(2) for biomedical imaging with >30,000,000-fold NMR signal enhancement in water. Anal. Chem [print-electronic]. 2014 Jun 6/17/2014; 86(12): 5601-5. PMID: 24738968, PMCID: PMC4063326, DOI: 10.1021/ac500952z, ISSN: 1520-6882.

Nikolaou P, Coffey AM, Walkup LL, Gust BM, Whiting N, Newton H, Muradyan I, Dabaghyan M, Ranta K, Moroz GD, Rosen MS, Patz S, Barlow MJ, Chekmenev EY, Goodson BM. XeNA: an automated 'open-source' (129)Xe hyperpolarizer for clinical use. Magn Reson Imaging [print-electronic]. 2014 Jun; 32(5): 541-50. PMID: 24631715, PMCID: PMC4011489, PII: S0730-725X(14)00045-9, DOI: 10.1016/j.mri.2014.02.002, ISSN: 1873-5894.

Nikolaou P, Coffey AM, Ranta K, Walkup LL, Gust BM, Barlow MJ, Rosen MS, Goodson BM, Chekmenev EY. Multidimensional mapping of spin-exchange optical pumping in clinical-scale batch-mode 129Xe hyperpolarizers. J Phys Chem B [print-electronic]. 2014 May 5/8/2014; 118(18): 4809-16. PMID: 24731261, PMCID: PMC4055050, DOI: 10.1021/jp501493k, ISSN: 1520-5207.

Perrin BS, Tian Y, Fu R, Grant CV, Chekmenev EY, Wieczorek WE, Dao AE, Hayden RM, Burzynski CM, Venable RM, Sharma M, Opella SJ, Pastor RW, Cotten ML. High-resolution structures and orientations of antimicrobial peptides piscidin 1 and piscidin 3 in fluid bilayers reveal tilting, kinking, and bilayer immersion. J. Am. Chem. Soc [print-electronic]. 2014 Mar 3/5/2014; 136(9): 3491-504. PMID: 24410116, PMCID: PMC3985945, DOI: 10.1021/ja411119m, ISSN: 1520-5126.

Barskiy DA, Kovtunov KV, Koptyug IV, He P, Groome KA, Best QA, Shi F, Goodson BM, Shchepin RV, Coffey AM, Waddell KW, Chekmenev EY. The feasibility of formation and kinetics of NMR signal amplification by reversible exchange (SABRE) at high magnetic field (9.4 T). J. Am. Chem. Soc [print-electronic]. 2014 Mar 3/5/2014; 136(9): 3322-5. PMID: 24528143, PMCID: PMC3985893, DOI: 10.1021/ja501052p, ISSN: 1520-5126.

Nikolaou P, Coffey AM, Walkup LL, Gust BM, LaPierre CD, Koehnemann E, Barlow MJ, Rosen MS, Goodson BM, Chekmenev EY. A 3D-printed high power nuclear spin polarizer. J. Am. Chem. Soc [print-electronic]. 2014 Jan 1/29/2014; 136(4): 1636-42. PMID: 24400919, PMCID: PMC4287367, DOI: 10.1021/ja412093d, ISSN: 1520-5126.

Coffey AM, Truong ML, Chekmenev EY. Low-field MRI can be more sensitive than high-field MRI. J. Magn. Reson [print-electronic]. 2013 Dec; 237: 169-74. PMID: 24239701, PMCID: PMC3897717, PII: S1090-7807(13)00268-1, DOI: 10.1016/j.jmr.2013.10.013, ISSN: 1096-0856.

Shchepin RV, Chekmenev EY. Synthetic approach for unsaturated precursors for parahydrogen induced polarization of choline and its analogs. J Labelled Comp Radiopharm [print-electronic]. 2013 Nov; 56(13): 655-62. PMID: 25196027, PMCID: PMC4159776, DOI: 10.1002/jlcr.3082, ISSN: 1099-1344.

Nikolaou P, Coffey AM, Walkup LL, Gust BM, Whiting N, Newton H, Barcus S, Muradyan I, Dabaghyan M, Moroz GD, Rosen MS, Patz S, Barlow MJ, Chekmenev EY, Goodson BM. Near-unity nuclear polarization with an open-source 129Xe hyperpolarizer for NMR and MRI. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 Aug 8/27/2013; 110(35): 14150-5. PMID: 23946420, PMCID: PMC3761567, PII: 1306586110, DOI: 10.1073/pnas.1306586110, ISSN: 1091-6490.

Cai C, Coffey AM, Shchepin RV, Chekmenev EY, Waddell KW. Efficient transformation of parahydrogen spin order into heteronuclear magnetization. J Phys Chem B [print-electronic]. 2013 Feb 2/7/2013; 117(5): 1219-24. PMID: 23214962, PMCID: PMC4287364, DOI: 10.1021/jp3089462, ISSN: 1520-5207.

Chekmenev, EY. MRI Hyperpolarization and Molecular Imaging. 2013.

Shchepin RV, Coffey AM, Waddell KW, Chekmenev EY. Parahydrogen Induced Polarization with Rh-based Monodentate Ligand in Water. J Phys Chem Lett. 2012 Oct 10/23/2012; 3(22): 3281-5. PMID: 23227297, PMCID: PMC3515067, DOI: 10.1021/jz301389r, ISSN: 1948-7185.

Abramson RG, Arlinghaus LR, Weis JA, Li X, Dula AN, Chekmenev EY, Smith SA, Miga MI, Abramson VG, Yankeelov TE. Current and emerging quantitative magnetic resonance imaging methods for assessing and predicting the response of breast cancer to neoadjuvant therapy. Breast Cancer (Dove Med Press). 2012 Oct; 2012(4): 139-54. PMID: 23154619, PMCID: PMC3496377, DOI: 10.2147/BCTT.S35882, ISSN: 1179-1314.

Coffey AM, Shchepin RV, Wilkens K, Waddell KW, Chekmenev EY. A large volume double channel 1H-X RF probe for hyperpolarized magnetic resonance at 0.0475 T. J. Magn. Reson [print-electronic]. 2012 Jul; 220: 94-101. PMID: 22706029, PMCID: PMC3510702, PII: S1090-7807(12)00158-9, DOI: 10.1016/j.jmr.2012.04.012, ISSN: 1096-0856.

Shchepin RV, Coffey AM, Waddell KW, Chekmenev EY. PASADENA hyperpolarized 13C phospholactate. J. Am. Chem. Soc [print-electronic]. 2012 Mar 3/7/2012; 134(9): 3957-60. PMID: 22352377, PMCID: PMC3318994, DOI: 10.1021/ja210639c, ISSN: 1520-5126.

Feng B, Coffey AM, Colon RD, Chekmenev EY, Waddell KW. A pulsed injection parahydrogen generator and techniques for quantifying enrichment. J. Magn. Reson [print-electronic]. 2012 Jan; 214(1): 258-62. PMID: 22188975, PMCID: PMC3268554, PII: S1090-7807(11)00483-6, DOI: 10.1016/j.jmr.2011.11.015, ISSN: 1096-0856.

Bhattacharya P, Chekmenev EY, Reynolds WF, Wagner S, Zacharias N, Chan HR, Bünger R, Ross BD. Parahydrogen-induced polarization (PHIP) hyperpolarized MR receptor imaging in vivo: a pilot study of 13C imaging of atheroma in mice. NMR Biomed [print-electronic]. 2011 Oct; 24(8): 1023-8. PMID: 21538638, PMCID: PMC3240663, DOI: 10.1002/nbm.1717, ISSN: 1099-1492.

Harrington MG, Chekmenev EY, Schepkin V, Fonteh AN, Arakaki X. Sodium MRI in a rat migraine model and a NEURON simulation study support a role for sodium in migraine. Cephalalgia [print-electronic]. 2011 Sep; 31(12): 1254-65. PMID: 21816771, PMCID: PMC3474349, PII: 0333102411408360, DOI: 10.1177/0333102411408360, ISSN: 1468-2982.

Kurhanewicz J, Vigneron DB, Brindle K, Chekmenev EY, Comment A, Cunningham CH, Deberardinis RJ, Green GG, Leach MO, Rajan SS, Rizi RR, Ross BD, Warren WS, Malloy CR. Analysis of cancer metabolism by imaging hyperpolarized nuclei: prospects for translation to clinical research. Neoplasia. 2011 Feb; 13(2): 81-97. PMID: 21403835, PMCID: PMC3033588, ISSN: 1476-5586.

Waddell KW, Coffey AM, Chekmenev EY. In situ detection of PHIP at 48 mT: demonstration using a centrally controlled polarizer. J. Am. Chem. Soc [print-electronic]. 2011 Jan 1/12/2011; 133(1): 97-101. PMID: 21141960, PMCID: PMC3098502, DOI: 10.1021/ja108529m, ISSN: 1520-5126.

Chekmenev EY, Vollmar BS, Cotten M. Can antimicrobial peptides scavenge around a cell in less than a second?. Biochim. Biophys. Acta [print-electronic]. 2010 Feb; 1798(2): 228-34. PMID: 19735645, PII: S0005-2736(09)00299-5, DOI: 10.1016/j.bbamem.2009.08.018, ISSN: 0006-3002.

Lisitza N, Muradian I, Frederick E, Patz S, Hatabu H, Chekmenev EY. Toward 13C hyperpolarized biomarkers produced by thermal mixing with hyperpolarized 129Xe. J Chem Phys. 2009 Jul 7/28/2009; 131(4): 44508. PMID: 19655895, PMCID: PMC2730707, DOI: 10.1063/1.3181062, ISSN: 1089-7690.

Hövener JB, Chekmenev EY, Harris KC, Perman WH, Tran TT, Ross BD, Bhattacharya P. Quality assurance of PASADENA hyperpolarization for 13C biomolecules. MAGMA [print-electronic]. 2009 Apr; 22(2): 123-34. PMID: 19067009, PMCID: PMC2664864, DOI: 10.1007/s10334-008-0154-y, ISSN: 1352-8661.

Hövener JB, Chekmenev EY, Harris KC, Perman WH, Robertson LW, Ross BD, Bhattacharya P. PASADENA hyperpolarization of 13C biomolecules: equipment design and installation. MAGMA [print-electronic]. 2009 Apr; 22(2): 111-21. PMID: 19067008, PMCID: PMC2664858, DOI: 10.1007/s10334-008-0155-x, ISSN: 1352-8661.

Chekmenev EY, Norton VA, Weitekamp DP, Bhattacharya P. Hyperpolarized (1)H NMR employing low gamma nucleus for spin polarization storage. J. Am. Chem. Soc. 2009 Mar 3/11/2009; 131(9): 3164-5. PMID: 19256566, PMCID: PMC2662390, DOI: 10.1021/ja809634u, ISSN: 1520-5126.

Chekmenev EY, Chow SK, Tofan D, Weitekamp DP, Ross BD, Bhattacharya P. Fluorine-19 NMR chemical shift probes molecular binding to lipid membranes. J Phys Chem B [print-electronic]. 2008 May 5/22/2008; 112(20): 6285-7. PMID: 18422359, PMCID: PMC2663341, DOI: 10.1021/jp800646k, ISSN: 1520-6106.

Chekmenev EY, Hövener J, Norton VA, Harris K, Batchelder LS, Bhattacharya P, Ross BD, Weitekamp DP. PASADENA hyperpolarization of succinic acid for MRI and NMR spectroscopy. J. Am. Chem. Soc [print-electronic]. 2008 Apr 4/2/2008; 130(13): 4212-3. PMID: 18335934, PMCID: PMC2662769, DOI: 10.1021/ja7101218, ISSN: 1520-5126.

Chekmenev, EY, Bhattacharya, P, Ross, BD. Development of Hyperpolarized Metabolic Contrast Agents using PASADENA. Cambridge Isotope Laboratories. 2008; Application Note: #21.

Gor'kov PL, Witter R, Chekmenev EY, Nozirov F, Fu R, Brey WW. Low-E probe for (19)F-(1)H NMR of dilute biological solids. J. Magn. Reson [print-electronic]. 2007 Dec; 189(2): 182-9. PMID: 17920316, PII: S1090-7807(07)00276-5, DOI: 10.1016/j.jmr.2007.09.008, ISSN: 1090-7807.

Bhattacharya P, Chekmenev EY, Perman WH, Harris KC, Lin AP, Norton VA, Tan CT, Ross BD, Weitekamp DP. Towards hyperpolarized (13)C-succinate imaging of brain cancer. J. Magn. Reson [print-electronic]. 2007 May; 186(1): 150-5. PMID: 17303454, PMCID: PMC2657725, PII: S1090-7807(07)00030-4, DOI: 10.1016/j.jmr.2007.01.017, ISSN: 1090-7807.

Gor'kov PL, Chekmenev EY, Li C, Cotten M, Buffy JJ, Traaseth NJ, Veglia G, Brey WW. Using low-E resonators to reduce RF heating in biological samples for static solid-state NMR up to 900 MHz. J. Magn. Reson [print-electronic]. 2007 Mar; 185(1): 77-93. PMID: 17174130, PII: S1090-7807(06)00379-X, DOI: 10.1016/j.jmr.2006.11.008, ISSN: 1090-7807.

Waddell KW, Chekmenev EY, Wittebort RJ. Peptide 17O chemical shielding and electric field gradient tensors. J Phys Chem B. 2006 Nov 11/16/2006; 110(45): 22935-41. PMID: 17092047, DOI: 10.1021/jp060617o, ISSN: 1520-6106.

Chekmenev EY, Gor'kov PL, Cross TA, Alaouie AM, Smirnov AI. Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy. Biophys. J [print-electronic]. 2006 Oct 10/15/2006; 91(8): 3076-84. PMID: 16861277, PMCID: PMC1578476, PII: S0006-3495(06)72021-5, DOI: 10.1529/biophysj.106.085191, ISSN: 0006-3495.

Buffy JJ, Traaseth NJ, Mascioni A, Gor'kov PL, Chekmenev EY, Brey WW, Veglia G. Two-dimensional solid-state NMR reveals two topologies of sarcolipin in oriented lipid bilayers. Biochemistry. 2006 Sep 9/12/2006; 45(36): 10939-46. PMID: 16953579, DOI: 10.1021/bi060728d, ISSN: 0006-2960.

Chekmenev EY, Vollmar BS, Forseth KT, Manion MN, Jones SM, Wagner TJ, Endicott RM, Kyriss BP, Homem LM, Pate M, He J, Raines J, Gor'kov PL, Brey WW, Mitchell DJ, Auman AJ, Ellard-Ivey MJ, Blazyk J, Cotten M. Investigating molecular recognition and biological function at interfaces using piscidins, antimicrobial peptides from fish. Biochim. Biophys. Acta [print-electronic]. 2006 Sep; 1758(9): 1359-72. PMID: 16815244, PII: S0005-2736(06)00130-1, DOI: 10.1016/j.bbamem.2006.03.034, ISSN: 0006-3002.

Chekmenev EY, Waddell KW, Hu J, Gan Z, Wittebort RJ, Cross TA. Ion-binding study by 17O solid-state NMR spectroscopy in the model peptide Gly-Gly-Gly at 19.6 T. J. Am. Chem. Soc. 2006 Aug 8/2/2006; 128(30): 9849-55. PMID: 16866542, DOI: 10.1021/ja060191r, ISSN: 0002-7863.

Gor'kov PL, Chekmenev EY, Fu R, Hu J, Cross TA, Cotten M, Brey WW. A large volume flat coil probe for oriented membrane proteins. J. Magn. Reson [print-electronic]. 2006 Jul; 181(1): 9-20. PMID: 16580852, PII: S1090-7807(06)00064-4, DOI: 10.1016/j.jmr.2006.03.008, ISSN: 1090-7807.

Li C, Mo Y, Hu J, Chekmenev E, Tian C, Gao FP, Fu R, Gor'kov P, Brey W, Cross TA. Analysis of RF heating and sample stability in aligned static solid-state NMR spectroscopy. J. Magn. Reson [print-electronic]. 2006 May; 180(1): 51-7. PMID: 16483809, PII: S1090-7807(06)00010-3, DOI: 10.1016/j.jmr.2006.01.013, ISSN: 1090-7807.

Chekmenev EY, Jones SM, Nikolayeva YN, Vollmar BS, Wagner TJ, Gor'kov PL, Brey WW, Manion MN, Daugherty KC, Cotten M. High-field NMR studies of molecular recognition and structure-function relationships in antimicrobial piscidins at the water-lipid bilayer interface. J. Am. Chem. Soc. 2006 Apr 4/26/2006; 128(16): 5308-9. PMID: 16620079, DOI: 10.1021/ja058385e, ISSN: 0002-7863.

Hu, J, Chekmenev, EY, Cross, TA. Modern Magnetic Resonance. [place unknown: publisher unknown]; 2006. (3). Available from: http://www.springerlink.com/content/x3986j97k3014148/?p=2fe96449eb294815863b57e84943af34&pi=1.

Fu R, Brey WW, Shetty K, Gor'kov P, Saha S, Long JR, Grant SC, Chekmenev EY, Hu J, Gan Z, Sharma M, Zhang F, Logan TM, Brüschweller R, Edison A, Blue A, Dixon IR, Markiewicz WD, Cross TA. Ultra-wide bore 900 MHz high-resolution NMR at the National High Magnetic Field Laboratory. J. Magn. Reson. 2005 Nov; 177(1): 1-8. PMID: 16125429, PII: S1090-7807(05)00227-2, DOI: 10.1016/j.jmr.2005.07.013, ISSN: 1090-7807.

Hu J, Chekmenev EY, Gan Z, Gor'kov PL, Saha S, Brey WW, Cross TA. Ion solvation by channel carbonyls characterized by 17O solid-state NMR at 21 T. J. Am. Chem. Soc. 2005 Aug 8/31/2005; 127(34): 11922-3. PMID: 16117514, DOI: 10.1021/ja0535413, ISSN: 0002-7863.

Waddell KW, Chekmenev EY, Wittebort RJ. Single-crystal studies of peptide prolyl and glycyl 15N shielding tensors. J. Am. Chem. Soc. 2005 Jun 6/29/2005; 127(25): 9030-5. PMID: 15969580, DOI: 10.1021/ja044204h, ISSN: 0002-7863.

Chekmenev EY, Hu J, Gor'kov PL, Brey WW, Cross TA, Ruuge A, Smirnov AI. 15N and 31P solid-state NMR study of transmembrane domain alignment of M2 protein of influenza A virus in hydrated cylindrical lipid bilayers confined to anodic aluminum oxide nanopores. J. Magn. Reson. 2005 Apr; 173(2): 322-7. PMID: 15780925, PII: S1090-7807(04)00413-6, DOI: 10.1016/j.jmr.2004.12.006, ISSN: 1090-7807.

Pometun MS, Chekmenev EY, Wittebort RJ. Quantitative observation of backbone disorder in native elastin. J. Biol. Chem [print-electronic]. 2004 Feb 2/27/2004; 279(9): 7982-7. PMID: 14625282, PII: M310948200, DOI: 10.1074/jbc.M310948200, ISSN: 0021-9258.

Chekmenev EY, Zhang Q, Waddell KW, Mashuta MS, Wittebort RJ. 15N Chemical shielding in glycyl tripeptides: measurement by solid-state NMR and correlation with X-ray structure. J. Am. Chem. Soc. 2004 Jan 1/14/2004; 126(1): 379-84. PMID: 14709105, DOI: 10.1021/ja0370342, ISSN: 0002-7863.

Fan, T. W. M.; Lane, A. N.; Chekmenev, E.; Wittebort, R. J.; Higashi, R. M.. Synthesis and physico-chemical properties of peptides in soil humic substances. J. Peptide Res. 2004; 63((3)): 253-64.

Zhang Q, Chekmenev EY, Wittebort RJ. 17O quadrupole coupling and chemical shielding tensors in an H-bonded carboxyl group: alpha-oxalic acid. J. Am. Chem. Soc. 2003 Jul 7/30/2003; 125(30): 9140-6. PMID: 15369371, DOI: 10.1021/ja034495e, ISSN: 0002-7863.

Chekmenev EY, Xu RZ, Mashuta MS, Wittebort RJ. Glycyl C(alpha) chemical shielding in tripeptides: measurement by solid-state NMR and correlation with X-ray structure and theory. J. Am. Chem. Soc. 2002 Oct 10/9/2002; 124(40): 11894-9. PMID: 12358533, PII: ja026700g, ISSN: 0002-7863.