Jorn-Hendrik Weitkamp, M.D.

Associate Professor

hendrik.weitkamp@vanderbilt.edu
Faculty Appointments
Associate Professor of Pediatrics
Education
M.D., Medicine, Universität Ulm, Ulm, Germany
Office Address
1125 MRB IV / Light Hall

2215 B Garland Ave
Nashville, TN 37232-9566
Research Description
Necrotizing entercolitis (NEC) is the most common gastrointestinal emergency in premature infants. Immaturity of gastrointestinal host defenses and deregulated immunological and inflammatory responses are major factors in NEC. FOXP3-positive regulatory T cells (Tregs) are specialized CD4+ T cells that regulate innate and adaptive immune responses. Very little data exist on the ontogeny of Tregs in the murine and human gastrointestinal tract.

We developed a reliable and efficient method for the isolation of intestinal lamina propria mononuclear cells from surgically resected intestinal tissue samples from infants with and without NEC. We are using flow cytometry, cytokine and T cell suppression assays, and molecular techniques to analyze phenotypic and functional properties of these cells.

By studying the development of effector and regulatory T cells in human infants, we will gain knowledge about gastrointestinal immune development, its role in neonatal and adult disease pathogenesis and how it is influenced by environmental factors such as nutrition and bacterial colonization.
Research Keywords
Gastrointestinal immune development; necrotizing enterocolitis (NEC); FOXP3; immunology; infectious disease; neonatology
Clinical Research Keywords
Neonatal infectious diseases, sepsis marker, health-care associated infections, quality improvement
Publications
Weitkamp JH, Koyama T, Rock MT, Correa H, Goettel JA, Matta P, Oswald-Richter K, Rosen MJ, Engelhardt BG, Moore DJ, Polk DB. Necrotising enterocolitis is characterised by disrupted immune regulation and diminished mucosal regulatory (FOXP3)/effector (CD4, CD8) T cell ratios. Gut [print-electronic]. 2013 Jan; 62(1): 73-82. PMID: 22267598, PMCID: PMC3606820, PII: gutjnl-2011-301551, DOI: 10.1136/gutjnl-2011-301551, ISSN: 1468-3288.

MohanKumar K, Kaza N, Jagadeeswaran R, Garzon SA, Bansal A, Kurundkar AR, Namachivayam K, Remon JI, Bandepalli CR, Feng X, Weitkamp JH, Maheshwari A. Gut mucosal injury in neonates is marked by macrophage infiltration in contrast to pleomorphic infiltrates in adult: evidence from an animal model. Am. J. Physiol. Gastrointest. Liver Physiol [print-electronic]. 2012 Jul; 303(1): G93-102. PMID: 22538401, PMCID: PMC3404576, PII: ajpgi.00016.2012, DOI: 10.1152/ajpgi.00016.2012, ISSN: 1522-1547.

Zhang C, Sherman MP, Prince LS, Bader D, Weitkamp JH, Slaughter JC, McElroy SJ. Paneth cell ablation in the presence of Klebsiella pneumoniae induces necrotizing enterocolitis (NEC)-like injury in the small intestine of immature mice. Dis Model Mech [print-electronic]. 2012 Jul; 5(4): 522-32. PMID: 22328592, PMCID: PMC3380715, PII: dmm.009001, DOI: 10.1242/dmm.009001, ISSN: 1754-8411.

Plosa EJ, Esbenshade JC, Fuller MP, Weitkamp JH. Cytomegalovirus infection. Pediatr Rev. 2012 Apr; 33(4): 156-63; quiz 163. PMID: 22474112, PII: 33/4/156, DOI: 10.1542/pir.33-4-156, ISSN: 1526-3347.

Romano-Keeler J, Weitkamp JH, Moore DJ. Regulatory properties of the intestinal microbiome effecting the development and treatment of diabetes. Curr Opin Endocrinol Diabetes Obes. 2012 Apr; 19(2): 73-80. PMID: 22357099, PMCID: PMC3560352, DOI: 10.1097/MED.0b013e3283514d43, ISSN: 1752-2978.

Ullrich T, Tang YW, Correa H, Garzon SA, Maheshwari A, Hill M, Matta P, Krishnan MK, Weitkamp JH. Absence of gastrointestinal pathogens in ileum tissue resected for necrotizing enterocolitis. Pediatr. Infect. Dis. J. 2012 Apr; 31(4): 413-4. PMID: 22173144, PMCID: PMC3305842, DOI: 10.1097/INF.0b013e318242534a, ISSN: 1532-0987.

Engelhardt BG, Jagasia SM, Crowe JE, Griffith ML, Savani BN, Kassim AA, Lu P, Weitkamp JH, Moore DJ, Yoder SM, Rock MT, Jagasia M. Predicting posttransplantation diabetes mellitus by regulatory T-cell phenotype: implications for metabolic intervention to modulate alloreactivity. Blood [print-electronic]. 2012 Mar 3/8/2012; 119(10): 2417-21. PMID: 22262764, PMCID: PMC3311262, PII: blood-2011-10-384750, DOI: 10.1182/blood-2011-10-384750, ISSN: 1528-0020.

Smith PB, Cohen-Wolkowiez M, Castro LM, Poindexter B, Bidegain M, Weitkamp JH, Schelonka RL, Ward RM, Wade K, Valencia G, Burchfield D, Arrieta A, Bhatt-Mehta V, Walsh M, Kantak A, Rasmussen M, Sullivan JE, Finer N, Brozanski BS, Sanchez P, van den Anker J, Blumer J, Kearns GL, Capparelli EV, Anand R, Benjamin DK, . Population pharmacokinetics of meropenem in plasma and cerebrospinal fluid of infants with suspected or complicated intra-abdominal infections. Pediatr. Infect. Dis. J. 2011 Oct; 30(10): 844-9. PMID: 21829139, PMCID: PMC3173561, DOI: 10.1097/INF.0b013e31822e8b0b, ISSN: 1532-0987.

McElroy SJ, Prince LS, Weitkamp JH, Reese J, Slaughter JC, Polk DB. Tumor necrosis factor receptor 1-dependent depletion of mucus in immature small intestine: a potential role in neonatal necrotizing enterocolitis. Am. J. Physiol. Gastrointest. Liver Physiol [print-electronic]. 2011 Oct; 301(4): G656-66. PMID: 21737776, PMCID: PMC3191555, PII: ajpgi.00550.2010, DOI: 10.1152/ajpgi.00550.2010, ISSN: 1522-1547.

Lytkin NI, McVoy L, Weitkamp JH, Aliferis CF, Statnikov A. Expanding the understanding of biases in development of clinical-grade molecular signatures: a case study in acute respiratory viral infections. PLoS ONE [print-electronic]. 2011; 6(6): e20662. PMID: 21673802, PMCID: PMC3105991, PII: PONE-D-11-01252, DOI: 10.1371/journal.pone.0020662, ISSN: 1932-6203.

Halpern MD, Weitkamp JH, Mount Patrick SK, Dobrenen HJ, Khailova L, Correa H, Dvorak B. Apical sodium-dependent bile acid transporter upregulation is associated with necrotizing enterocolitis. Am. J. Physiol. Gastrointest. Liver Physiol [print-electronic]. 2010 Sep; 299(3): G623-31. PMID: 20616306, PMCID: PMC2950692, PII: ajpgi.00242.2010, DOI: 10.1152/ajpgi.00242.2010, ISSN: 1522-1547.

Hill ML, Baldwin L, Slaughter JC, Walsh WF, Weitkamp JH. A silver-alginate-coated dressing to reduce peripherally inserted central catheter (PICC) infections in NICU patients: a pilot randomized controlled trial. J Perinatol [print-electronic]. 2010 Jul; 30(7): 469-73. PMID: 20010613, PII: jp2009190, DOI: 10.1038/jp.2009.190, ISSN: 1476-5543.

Statnikov A, Lytkin NI, McVoy L, Weitkamp JH, Aliferis CF. Using gene expression profiles from peripheral blood to identify asymptomatic responses to acute respiratory viral infections. BMC Res Notes. 2010; 3: 264. PMID: 20961438, PMCID: PMC2975649, PII: 1756-0500-3-264, DOI: 10.1186/1756-0500-3-264, ISSN: 1756-0500.

Williams JV, Weitkamp JH, Blum DL, LaFleur BJ, Crowe JE. The human neonatal B cell response to respiratory syncytial virus uses a biased antibody variable gene repertoire that lacks somatic mutations. Mol. Immunol [print-electronic]. 2009 Dec; 47(2-3): 407-14. PMID: 19804909, PMCID: PMC2788105, PII: S0161-5890(09)00701-9, DOI: 10.1016/j.molimm.2009.08.024, ISSN: 1872-9142.

Weitkamp JH, Rudzinski E, Koyama T, Correa H, Matta P, Alberty B, Polk DB. Ontogeny of FOXP3(+) regulatory T cells in the postnatal human small intestinal and large intestinal lamina propria. Pediatr. Dev. Pathol. 2009 Nov; 12(6): 443-9. PMID: 19203136, PMCID: PMC2844857, PII: 08-09-0533, DOI: 10.2350/08-09-0533.1, ISSN: 1093-5266.

Weitkamp JH, Ozdas A, LaFleur B, Potts AL. Fluconazole prophylaxis for prevention of invasive fungal infections in targeted highest risk preterm infants limits drug exposure. J Perinatol [print-electronic]. 2008 Jun; 28(6): 405-11. PMID: 18185518, PII: 7211914, DOI: 10.1038/sj.jp.7211914, ISSN: 0743-8346.

Sparks JR, Recchia FM, Weitkamp JH. Endogenous group B streptococcal endophthalmitis in a preterm infant. J Perinatol. 2007 Jun; 27(6): 392-4. PMID: 17522689, PII: 7211685, DOI: 10.1038/sj.jp.7211685, ISSN: 0743-8346.

Choudhury SA, Berthaud V, Weitkamp JH. Meningitis caused by Salmonella panama in infants. J Natl Med Assoc. 2006 Feb; 98(2): 219-22. PMID: 16708507, PMCID: PMC2595037, ISSN: 1943-4693.

Weitkamp JH, Lafleur BJ, Crowe JE. Rotavirus-specific CD5+ B cells in young children exhibit a distinct antibody repertoire compared with CD5- B cells. Hum. Immunol [print-electronic]. 2006 Jan; 67(1-2): 33-42. PMID: 16698423, PII: S0198-8859(06)00011-5, DOI: 10.1016/j.humimm.2006.02.024, ISSN: 0198-8859.

Weitkamp JH, Lafleur BJ, Greenberg HB, Crowe JE. Natural evolution of a human virus-specific antibody gene repertoire by somatic hypermutation requires both hotspot-directed and randomly-directed processes. Hum. Immunol. 2005 Jun; 66(6): 666-76. PMID: 15993712, PII: S0198-8859(05)00060-1, DOI: 10.1016/j.humimm.2005.02.008, ISSN: 0198-8859.

Weitkamp JH, Kallewaard NL, Bowen AL, Lafleur BJ, Greenberg HB, Crowe JE. VH1-46 is the dominant immunoglobulin heavy chain gene segment in rotavirus-specific memory B cells expressing the intestinal homing receptor alpha4beta7. J. Immunol. 2005 Mar 3/15/2005; 174(6): 3454-60. PMID: 15749880, PII: 174/6/3454, ISSN: 0022-1767.

Weitkamp JH, Spring MD, Brogan T, Moses H, Bloch KC, Wright PF. Influenza A virus-associated acute necrotizing encephalopathy in the United States. Pediatr. Infect. Dis. J. 2004 Mar; 23(3): 259-63. PMID: 15014305, PII: 00006454-200403000-00019, ISSN: 0891-3668.

Weitkamp JH, Kallewaard N, Kusuhara K, Bures E, Williams JV, LaFleur B, Greenberg HB, Crowe JE. Infant and adult human B cell responses to rotavirus share common immunodominant variable gene repertoires. J. Immunol. 2003 Nov 11/1/2003; 171(9): 4680-8. PMID: 14568943, ISSN: 0022-1767.

Weitkamp JH, Kallewaard N, Kusuhara K, Feigelstock D, Feng N, Greenberg HB, Crowe JE. Generation of recombinant human monoclonal antibodies to rotavirus from single antigen-specific B cells selected with fluorescent virus-like particles. J. Immunol. Methods. 2003 Apr 4/1/2003; 275(1-2): 223-37. PMID: 12667686, PII: S0022175903000139, ISSN: 0022-1759.

Weitkamp JH, Reinsberg J, Bartmann P. Interleukin-8 (IL-8) preferable to IL-6 as a marker for clinical infection [letter]. Clin. Diagn. Lab. Immunol. 2002 Nov; 9(6): 1401. PMID: 12414787, PMCID: PMC130121, ISSN: 1071-412X.

Weitkamp JH, Crowe JE. Blood donor leukocyte reduction filters as a source of human B lymphocytes. BioTechniques. 2001 Sep; 31(3): 464-6. PMID: 11570486, ISSN: 0736-6205.

Crowe JE, Suara RO, Brock S, Kallewaard N, House F, Weitkamp JH. Genetic and structural determinants of virus neutralizing antibodies. Immunol. Res. 2001; 23(2-3): 135-45. PMID: 11444379, PII: IR:23:2-3:135, DOI: 10.1385/IR:23:2-3:135, ISSN: 0257-277X.

Weitkamp JH, Tang YW, Haas DW, Midha NK, Crowe JE. Recurrent Achromobacter xylosoxidans bacteremia associated with persistent lymph node infection in a patient with hyper-immunoglobulin M syndrome. Clin. Infect. Dis [print-electronic]. 2000 Nov; 31(5): 1183-7. PMID: 11073750, PII: CID994327, DOI: 10.1086/317461, ISSN: 1058-4838.

Weitkamp JH, Stüber F, Bartmann P. Pilot study assessing TNF gene polymorphism as a prognostic marker for disease progression in neonates with sepsis. Infection. 2000 Mar; 28(2): 92-6. PMID: 10782394, ISSN: 0300-8126.

Weitkamp JH, Poets CF, Sievers R, Musswessels E, Groneck P, Thomas P, Bartmann P. Candida infection in very low birth-weight infants: outcome and nephrotoxicity of treatment with liposomal amphotericin B (AmBisome). Infection. 1998 Jan; 26(1): 11-5. PMID: 9505173, ISSN: 0300-8126.

Weitkamp JH, Pérez-Pérez GI, Bode G, Malfertheiner P, Blaser MJ. Identification and characterization of Helicobacter pylori phospholipase C activity. Zentralbl. Bakteriol. 1993 Sep; 280(1-2): 11-27. PMID: 8280931, ISSN: 0934-8840.

Available Postdoctoral Position Details
Posted: 5/22/2012
Motivated candidates with a strong background in immunology, cell biology, or molecular biology are encouraged to apply. We are looking for a dedicated and scientifically curious individual with research experience in flow cytometry, histochemistry, cell culture or signal transduction. Additional training as necessary will be provided.