Alyssa H. Hasty, Ph.D.


Visit Lab Site

Faculty Appointments
Professor of Molecular Physiology and Biophysics
Ph.D., Lipoprotein Metabolism / Atherosclerosis, Vanderbilt University, Nashville, TennesseeB.S., Cell Biology, Tennessee Technological University, Cookeville, Tennessee
Office Address
813 LH
23rd Ave South and Pierce
Nashville, TN 0615
Research Description
Research description:

We study how obesity impacts health. The growing worldwide obesity epidemic is frequently linked to hyperlipidemia, inflammation, and insulin resistance leading to increased risk of diabetes and cardiovascular disease. The long-term goal of our laboratory is to determine mechanisms by which obesity increases risk for and pathophysiological consequences of these devastating diseases. Macrophages are part of the innate immune system that infiltrate white adipose tissue (fat) in obese rodents and humans, and produce most of the inflammatory cytokines and chemokines secreted from adipose tissue. In addition, their presence has been shown to be temporally associated with the development of insulin resistance. Our current research focus is threefold: to determine mechanisms by which macrophages accumulate in adipose tissue, to determine the role of resident macrophages in normal adipose tissue function, and to determine how other immune cells like eosinophils also contribute to adipose tissue function.

Adipose Tissue Macrophage Turnover: We have growing evidence that even in lean adipose tissue the macrophages undergo apoptotic cell death. We would like to understand how the natural turnover of macrophages contributes to the overall numbers of cells in lean and obese adipose tissue. We hypothesize that this turnover may be impaired in obesity and contribute to the accumulation of macrophages in obese adipose tissue.

Adipose Tissue Macrophage Iron Metabolism: Not much is known about the role of adipose tissue macrophages in tissue homeostasis. Our recent data suggests that resident M2-like macrophages may play a role in iron metabolism in fat. These exciting findings have opened up a new area of research for our group to assess the impact of macrophage iron handling on adipose tissue homeostasis.

Other Immune Cells in Adipose Tissue: Many laboratories have shown the presence of other immune cells such as eosinophils, neutrophils, dendritic cells, T cells, B cells in adipose tissue. We are particularly interested in the role of eosinophils in adipose tissue homeostasis. We are also interested in the role of T cells in adipose tissue dysfunction seen with weight cycling.
Research Keywords
obesity, macrophages, adipose tissue, diabetes, immunometabolism, immunology, innate immunity, eosinophils, resolution, iron, apoptosis
Ong HH, Webb CD, Gruen ML, Hasty AH, Gore JC, Welch EB. Fat-water MRI of a diet-induced obesity mouse model at 15.2T. J Med Imaging (Bellingham) [print-electronic]. 2016 Apr; 3(2): 26002. PMID: 27226976, PMCID: PMC4877437, PII: 15190PRR, DOI: 10.1117/1.JMI.3.2.026002, ISSN: 2329-4302.

Bolus WR, Gutierrez DA, Kennedy AJ, Anderson-Baucum EK, Hasty AH. CCR2 deficiency leads to increased eosinophils, alternative macrophage activation, and type 2 cytokine expression in adipose tissue. J. Leukoc. Biol [print-electronic]. 2015 Oct; 98(4): 467-77. PMID: 25934927, PII: jlb.3HI0115-018R, DOI: 10.1189/jlb.3HI0115-018R, ISSN: 1938-3673.

Hill AA, Anderson-Baucum EK, Kennedy AJ, Webb CD, Yull FE, Hasty AH. Activation of NF-kB drives the enhanced survival of adipose tissue macrophages in an obesogenic environment. Mol Metab. 2015 Oct; 4(10): 665-77. PMID: 26779432, PMCID: PMC4588436, PII: S2212-8778(15)00139-8, DOI: 10.1016/j.molmet.2015.07.005, ISSN: 2212-8778.

Wainright KS, Fleming NJ, Rowles JL, Welly RJ, Zidon TM, Park YM, Gaines TL, Scroggins RJ, Anderson-Baucum EK, Hasty AH, Vieira-Potter VJ, Padilla J. Retention of sedentary obese visceral white adipose tissue phenotype with intermittent physical activity despite reduced adiposity. Am. J. Physiol. Regul. Integr. Comp. Physiol [print-electronic]. 2015 Sep; 309(5): R594-602. PMID: 26180183, PMCID: PMC4591377, PII: ajpregu.00042.2015, DOI: 10.1152/ajpregu.00042.2015, ISSN: 1522-1490.

Riley KG, Pasek RC, Maulis MF, Dunn JC, Bolus WR, Kendall PL, Hasty AH, Gannon M. Macrophages are essential for CTGF-mediated adult ß-cell proliferation after injury. Mol Metab. 2015 Aug; 4(8): 584-91. PMID: 26266091, PMCID: PMC4529497, PII: S2212-8778(15)00091-5, DOI: 10.1016/j.molmet.2015.05.002, ISSN: 2212-8778.

Hubler MJ, Peterson KR, Hasty AH. Iron homeostasis: a new job for macrophages in adipose tissue?. Trends Endocrinol. Metab [print-electronic]. 2015 Feb; 26(2): 101-9. PMID: 25600948, PMCID: PMC4315734, PII: S1043-2760(14)00216-1, DOI: 10.1016/j.tem.2014.12.005, ISSN: 1879-3061.

Hill AA, Reid Bolus W, Hasty AH. A decade of progress in adipose tissue macrophage biology. Immunol. Rev. 2014 Nov; 262(1): 134-52. PMID: 25319332, PMCID: PMC4203421, DOI: 10.1111/imr.12216, ISSN: 1600-065X.

Reddy IA, Wasserman DH, Ayala JE, Hasty AH, Abumrad NN, Galli A. Striatal dopamine homeostasis is altered in mice following Roux-en-Y gastric bypass surgery. ACS Chem Neurosci [print-electronic]. 2014 Oct 10/15/2014; 5(10): 943-51. PMID: 25068716, PMCID: PMC4324416, DOI: 10.1021/cn500137d, ISSN: 1948-7193.

Buckman LB, Anderson-Baucum EK, Hasty AH, Ellacott KLj. Regulation of S100B in white adipose tissue by obesity in mice. Adipocyte [print-electronic]. 2014 Jul 7/1/2014; 3(3): 215-20. PMID: 25068089, PMCID: PMC4110099, PII: 2014ADIPOCYTE167R, DOI: 10.4161/adip.28730, ISSN: 2162-3945.

Anderson-Baucum EK, Major AS, Hasty AH. A possible secondary immune response in adipose tissue during weight cycling: The ups and downs of yo-yo dieting. Adipocyte [print-electronic]. 2014 Apr 4/1/2014; 3(2): 141-5. PMID: 24719788, PMCID: PMC3979879, PII: 2013APIDOCYTE137R, DOI: 10.4161/adip.27556, ISSN: 2162-3945.

Orr JS, Kennedy A, Anderson-Baucum EK, Webb CD, Fordahl SC, Erikson KM, Zhang Y, Etzerodt A, Moestrup SK, Hasty AH. Obesity alters adipose tissue macrophage iron content and tissue iron distribution. Diabetes [print-electronic]. 2014 Feb; 63(2): 421-32. PMID: 24130337, PMCID: PMC3900546, PII: db13-0213, DOI: 10.2337/db13-0213, ISSN: 1939-327X.

Buckman LB, Hasty AH, Flaherty DK, Buckman CT, Thompson MM, Matlock BK, Weller K, Ellacott KL. Obesity induced by a high-fat diet is associated with increased immune cell entry into the central nervous system. Brain Behav. Immun [print-electronic]. 2014 Jan; 35: 33-42. PMID: 23831150, PMCID: PMC3858467, PII: S0889-1591(13)00231-6, DOI: 10.1016/j.bbi.2013.06.007, ISSN: 1090-2139.

Hasty AH, Gutierrez DA. What have we really learned about macrophage recruitment to adipose tissue?. Endocrinology. 2014 Jan; 155(1): 12-4. PMID: 24364586, PMCID: PMC3868801, DOI: 10.1210/en.2013-2027, ISSN: 1945-7170.

Hasty AH, Yvan-Charvet L. Liver X receptor a-dependent iron handling in M2 macrophages: The missing link between cholesterol and intraplaque hemorrhage? [editorial]. Circ. Res. 2013 Nov 11/8/2013; 113(11): 1182-5. PMID: 24201110, PII: CIRCRESAHA.113.302613, DOI: 10.1161/CIRCRESAHA.113.302613, ISSN: 1524-4571.

Kennedy A, Webb CD, Hill AA, Gruen ML, Jackson LG, Hasty AH. Loss of CCR5 results in glucose intolerance in diet-induced obese mice. Am. J. Physiol. Endocrinol. Metab [print-electronic]. 2013 Oct 10/1/2013; 305(7): E897-906. PMID: 23941876, PMCID: PMC3798705, PII: ajpendo.00177.2013, DOI: 10.1152/ajpendo.00177.2013, ISSN: 1522-1555.

Anderson EK, Gutierrez DA, Kennedy A, Hasty AH. Weight cycling increases T-cell accumulation in adipose tissue and impairs systemic glucose tolerance. Diabetes [print-electronic]. 2013 Sep; 62(9): 3180-8. PMID: 23733197, PMCID: PMC3749340, PII: db12-1076, DOI: 10.2337/db12-1076, ISSN: 1939-327X.

Kang L, Lantier L, Kennedy A, Bonner JS, Mayes WH, Bracy DP, Bookbinder LH, Hasty AH, Thompson CB, Wasserman DH. Hyaluronan accumulates with high-fat feeding and contributes to insulin resistance. Diabetes [print-electronic]. 2013 Jun; 62(6): 1888-96. PMID: 23349492, PMCID: PMC3661617, PII: db12-1502, DOI: 10.2337/db12-1502, ISSN: 1939-327X.

Orr JS, Kennedy AJ, Hasty AH. Isolation of adipose tissue immune cells. J Vis Exp. 2013; (75): e50707. PMID: 23728515, PMCID: PMC3718226, DOI: 10.3791/50707, ISSN: 1940-087X.

Orr JS, Puglisi MJ, Ellacott KL, Lumeng CN, Wasserman DH, Hasty AH. Toll-like receptor 4 deficiency promotes the alternative activation of adipose tissue macrophages. Diabetes [print-electronic]. 2012 Nov; 61(11): 2718-27. PMID: 22751700, PMCID: PMC3478520, PII: db11-1595, DOI: 10.2337/db11-1595, ISSN: 1939-327X.

Hasty AH, Harrison DG. Endoplasmic reticulum stress and hypertension - a new paradigm?. J. Clin. Invest [print-electronic]. 2012 Nov; 122(11): 3859-61. PMID: 23064369, PMCID: PMC3484081, PII: 65173, DOI: 10.1172/JCI65173, ISSN: 1558-8238.

Anderson EK, Hill AA, Hasty AH. Stearic acid accumulation in macrophages induces toll-like receptor 4/2-independent inflammation leading to endoplasmic reticulum stress-mediated apoptosis. Arterioscler. Thromb. Vasc. Biol [print-electronic]. 2012 Jul; 32(7): 1687-95. PMID: 22556332, PMCID: PMC3385654, PII: ATVBAHA.112.250142, DOI: 10.1161/ATVBAHA.112.250142, ISSN: 1524-4636.

Gutierrez DA, Hasty AH. Haematopoietic leptin receptor deficiency does not affect macrophage accumulation in adipose tissue or systemic insulin sensitivity. J. Endocrinol [print-electronic]. 2012 Mar; 212(3): 343-51. PMID: 22194312, PMCID: PMC3381898, PII: JOE-11-0338, DOI: 10.1530/JOE-11-0338, ISSN: 1479-6805.

Kennedy A, Gruen ML, Gutierrez DA, Surmi BK, Orr JS, Webb CD, Hasty AH. Impact of macrophage inflammatory protein-1a deficiency on atherosclerotic lesion formation, hepatic steatosis, and adipose tissue expansion. PLoS ONE [print-electronic]. 2012; 7(2): e31508. PMID: 22359597, PMCID: PMC3281060, PII: PONE-D-11-08027, DOI: 10.1371/journal.pone.0031508, ISSN: 1932-6203.

Gutierrez DA, Kennedy A, Orr JS, Anderson EK, Webb CD, Gerrald WK, Hasty AH. Aberrant accumulation of undifferentiated myeloid cells in the adipose tissue of CCR2-deficient mice delays improvements in insulin sensitivity. Diabetes [print-electronic]. 2011 Nov; 60(11): 2820-9. PMID: 21926275, PMCID: PMC3198070, PII: db11-0314, DOI: 10.2337/db11-0314, ISSN: 1939-327X.

Puglisi MJ, Hasty AH, Saraswathi V. The role of adipose tissue in mediating the beneficial effects of dietary fish oil. J. Nutr. Biochem [print-electronic]. 2011 Feb; 22(2): 101-8. PMID: 21145721, PMCID: PMC3038466, PII: S0955-2863(10)00166-X, DOI: 10.1016/j.jnutbio.2010.07.003, ISSN: 1873-4847.

Guri AJ, Misyak SA, Hontecillas R, Hasty A, Liu D, Si H, Bassaganya-Riera J. Abscisic acid ameliorates atherosclerosis by suppressing macrophage and CD4+ T cell recruitment into the aortic wall. J. Nutr. Biochem [print-electronic]. 2010 Dec; 21(12): 1178-85. PMID: 20092994, PMCID: PMC2891372, PII: S0955-2863(09)00216-2, DOI: 10.1016/j.jnutbio.2009.10.003, ISSN: 1873-4847.

Surmi BK, Webb CD, Ristau AC, Hasty AH. Absence of macrophage inflammatory protein-1{alpha} does not impact macrophage accumulation in adipose tissue of diet-induced obese mice. Am. J. Physiol. Endocrinol. Metab [print-electronic]. 2010 Sep; 299(3): E437-45. PMID: 20551286, PMCID: PMC2944285, PII: ajpendo.00050.2010, DOI: 10.1152/ajpendo.00050.2010, ISSN: 1522-1555.

Anderson EK, Gutierrez DA, Hasty AH. Adipose tissue recruitment of leukocytes. Curr. Opin. Lipidol. 2010 Jun; 21(3): 172-7. PMID: 20410821, PMCID: PMC3381420, DOI: 10.1097/MOL.0b013e3283393867, ISSN: 1473-6535.

Rudolph TK, Rudolph V, Edreira MM, Cole MP, Bonacci G, Schopfer FJ, Woodcock SR, Franek A, Pekarova M, Khoo NK, Hasty AH, Baldus S, Freeman BA. Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice. Arterioscler. Thromb. Vasc. Biol [print-electronic]. 2010 May; 30(5): 938-45. PMID: 20167658, PMCID: PMC2857965, PII: ATVBAHA.109.201582, DOI: 10.1161/ATVBAHA.109.201582, ISSN: 1524-4636.

Kennedy AJ, Ellacott KL, King VL, Hasty AH. Mouse models of the metabolic syndrome. Dis Model Mech. 2010 Mar; 3(3-4): 156-66. PMID: 20212084, PMCID: PMC2869491, PII: 3/3-4/156, DOI: 10.1242/dmm.003467, ISSN: 1754-8411.

Surmi BK, Hasty AH. The role of chemokines in recruitment of immune cells to the artery wall and adipose tissue. Vascul. Pharmacol [print-electronic]. 2010 Jan; 52(1-2): 27-36. PMID: 20026286, PMCID: PMC2823842, PII: S1537-1891(09)00159-1, DOI: 10.1016/j.vph.2009.12.004, ISSN: 1879-3649.

Saraswathi V, Morrow JD, Hasty AH. Dietary fish oil exerts hypolipidemic effects in lean and insulin sensitizing effects in obese LDLR-/- mice. J. Nutr [print-electronic]. 2009 Dec; 139(12): 2380-6. PMID: 19864403, PMCID: PMC2777481, PII: jn.109.111567, DOI: 10.3945/jn.109.111567, ISSN: 1541-6100.

Li S, Sun Y, Liang CP, Thorp EB, Han S, Jehle AW, Saraswathi V, Pridgen B, Kanter JE, Li R, Welch CL, Hasty AH, Bornfeldt KE, Breslow JL, Tabas I, Tall AR. Defective phagocytosis of apoptotic cells by macrophages in atherosclerotic lesions of ob/ob mice and reversal by a fish oil diet. Circ. Res [print-electronic]. 2009 Nov 11/20/2009; 105(11): 1072-82. PMID: 19834009, PMCID: PMC2818555, PII: CIRCRESAHA.109.199570, DOI: 10.1161/CIRCRESAHA.109.199570, ISSN: 1524-4571.

Saraswathi V, Hasty AH. Inhibition of long-chain acyl coenzyme A synthetases during fatty acid loading induces lipotoxicity in macrophages. Arterioscler. Thromb. Vasc. Biol [print-electronic]. 2009 Nov; 29(11): 1937-43. PMID: 19679826, PMCID: PMC2766024, PII: ATVBAHA.109.195362, DOI: 10.1161/ATVBAHA.109.195362, ISSN: 1524-4636.

Romero-Aleshire MJ, Diamond-Stanic MK, Hasty AH, Hoyer PB, Brooks HL. Loss of ovarian function in the VCD mouse-model of menopause leads to insulin resistance and a rapid progression into the metabolic syndrome. Am. J. Physiol. Regul. Integr. Comp. Physiol [print-electronic]. 2009 Sep; 297(3): R587-92. PMID: 19439618, PMCID: PMC2739794, PII: 90762.2008, DOI: 10.1152/ajpregu.90762.2008, ISSN: 1522-1490.

Batoy SM, Borgmann S, Flick K, Griffith J, Jones JJ, Saraswathi V, Hasty AH, Kaiser P, Wilkins CL. Lipid and phospholipid profiling of biological samples using MALDI Fourier transform mass spectrometry. Lipids [print-electronic]. 2009 Apr; 44(4): 367-71. PMID: 19005715, PMCID: PMC3572737, DOI: 10.1007/s11745-008-3260-8, ISSN: 1558-9307.

Gutierrez DA, Puglisi MJ, Hasty AH. Impact of increased adipose tissue mass on inflammation, insulin resistance, and dyslipidemia. Curr. Diab. Rep. 2009 Feb; 9(1): 26-32. PMID: 19192421, PMCID: PMC2735041, ISSN: 1539-0829.

Coenen KR, Gruen ML, Lee-Young RS, Puglisi MJ, Wasserman DH, Hasty AH. Impact of macrophage toll-like receptor 4 deficiency on macrophage infiltration into adipose tissue and the artery wall in mice. Diabetologia [print-electronic]. 2009 Feb; 52(2): 318-28. PMID: 19052722, PMCID: PMC2615827, DOI: 10.1007/s00125-008-1221-7, ISSN: 1432-0428.

Plummer MR, Hasty AH. Atherosclerotic lesion formation and triglyceride storage in obese apolipoprotein AI-deficient mice. J. Nutr. Biochem [print-electronic]. 2008 Oct; 19(10): 664-73. PMID: 18280133, PII: S0955-2863(07)00213-6, DOI: 10.1016/j.jnutbio.2007.08.009, ISSN: 0955-2863.

Kato T, Shimano H, Yamamoto T, Ishikawa M, Kumadaki S, Matsuzaka T, Nakagawa Y, Yahagi N, Nakakuki M, Hasty AH, Takeuchi Y, Kobayashi K, Takahashi A, Yatoh S, Suzuki H, Sone H, Yamada N. Palmitate impairs and eicosapentaenoate restores insulin secretion through regulation of SREBP-1c in pancreatic islets. Diabetes [print-electronic]. 2008 Sep; 57(9): 2382-92. PMID: 18458149, PMCID: PMC2518489, PII: db06-1806, DOI: 10.2337/db06-1806, ISSN: 1939-327X.

Inoue N, Yahagi N, Yamamoto T, Ishikawa M, Watanabe K, Matsuzaka T, Nakagawa Y, Takeuchi Y, Kobayashi K, Takahashi A, Suzuki H, Hasty AH, Toyoshima H, Yamada N, Shimano H. Cyclin-dependent kinase inhibitor, p21WAF1/CIP1, is involved in adipocyte differentiation and hypertrophy, linking to obesity, and insulin resistance. J. Biol. Chem [print-electronic]. 2008 Jul 7/25/2008; 283(30): 21220-9. PMID: 18445590, PMCID: PMC3258954, PII: M801824200, DOI: 10.1074/jbc.M801824200, ISSN: 0021-9258.

Stafford JM, Yu F, Printz R, Hasty AH, Swift LL, Niswender KD. Central nervous system neuropeptide Y signaling modulates VLDL triglyceride secretion. Diabetes [print-electronic]. 2008 Jun; 57(6): 1482-90. PMID: 18332095, PMCID: PMC3968924, PII: db07-1702, DOI: 10.2337/db07-1702, ISSN: 1939-327X.

Surmi BK, Atkinson RD, Gruen ML, Coenen KR, Hasty AH. The role of macrophage leptin receptor in aortic root lesion formation. Am. J. Physiol. Endocrinol. Metab [print-electronic]. 2008 Mar; 294(3): E488-95. PMID: 18182468, PII: 00374.2007, DOI: 10.1152/ajpendo.00374.2007, ISSN: 0193-1849.

Atkinson RD, Coenen KR, Plummer MR, Gruen ML, Hasty AH. Macrophage-derived apolipoprotein E ameliorates dyslipidemia and atherosclerosis in obese apolipoprotein E-deficient mice. Am. J. Physiol. Endocrinol. Metab [print-electronic]. 2008 Feb; 294(2): E284-90. PMID: 18029445, PII: 00601.2007, DOI: 10.1152/ajpendo.00601.2007, ISSN: 0193-1849.

Surmi BK, Hasty AH. Macrophage infiltration into adipose tissue: initiation, propagation and remodeling. Future Lipidol. 2008; 3(5): 545-56. PMID: 18978945, PMCID: PMC2575346, DOI: 10.2217/17460875.3.5.545, ISSN: 1746-0875.

Nuotio-Antar AM, Hachey DL, Hasty AH. Carbenoxolone treatment attenuates symptoms of metabolic syndrome and atherogenesis in obese, hyperlipidemic mice. Am. J. Physiol. Endocrinol. Metab [print-electronic]. 2007 Dec; 293(6): E1517-28. PMID: 17878220, PII: 00522.2007, DOI: 10.1152/ajpendo.00522.2007, ISSN: 0193-1849.

Gruen ML, Hao M, Piston DW, Hasty AH. Leptin requires canonical migratory signaling pathways for induction of monocyte and macrophage chemotaxis. Am. J. Physiol., Cell Physiol [print-electronic]. 2007 Nov; 293(5): C1481-8. PMID: 17728393, PII: 00062.2007, DOI: 10.1152/ajpcell.00062.2007, ISSN: 0363-6143.

Coenen KR, Gruen ML, Hasty AH. Obesity causes very low density lipoprotein clearance defects in low-density lipoprotein receptor-deficient mice. J. Nutr. Biochem [print-electronic]. 2007 Nov; 18(11): 727-35. PMID: 17418556, PII: S0955-2863(07)00018-6, DOI: 10.1016/j.jnutbio.2006.12.010, ISSN: 0955-2863.

Matsuzaka T, Shimano H, Yahagi N, Kato T, Atsumi A, Yamamoto T, Inoue N, Ishikawa M, Okada S, Ishigaki N, Iwasaki H, Iwasaki Y, Karasawa T, Kumadaki S, Matsui T, Sekiya M, Ohashi K, Hasty AH, Nakagawa Y, Takahashi A, Suzuki H, Yatoh S, Sone H, Toyoshima H, Osuga J, Yamada N. Crucial role of a long-chain fatty acid elongase, Elovl6, in obesity-induced insulin resistance. Nat. Med [print-electronic]. 2007 Oct; 13(10): 1193-202. PMID: 17906635, PII: nm1662, DOI: 10.1038/nm1662, ISSN: 1078-8956.

Hao M, Head WS, Gunawardana SC, Hasty AH, Piston DW. Direct effect of cholesterol on insulin secretion: a novel mechanism for pancreatic beta-cell dysfunction. Diabetes [print-electronic]. 2007 Sep; 56(9): 2328-38. PMID: 17575085, PII: db07-0056, DOI: 10.2337/db07-0056, ISSN: 1939-327X.

Coenen KR, Hasty AH. Obesity potentiates development of fatty liver and insulin resistance, but not atherosclerosis, in high-fat diet-fed agouti LDLR-deficient mice. Am. J. Physiol. Endocrinol. Metab [print-electronic]. 2007 Aug; 293(2): E492-9. PMID: 17566116, PII: 00171.2007, DOI: 10.1152/ajpendo.00171.2007, ISSN: 0193-1849.

Saraswathi V, Gao L, Morrow JD, Chait A, Niswender KD, Hasty AH. Fish oil increases cholesterol storage in white adipose tissue with concomitant decreases in inflammation, hepatic steatosis, and atherosclerosis in mice. J. Nutr. 2007 Jul; 137(7): 1776-82. PMID: 17585030, PII: 137/7/1776, ISSN: 0022-3166.

Coenen KR, Gruen ML, Chait A, Hasty AH. Diet-induced increases in adiposity, but not plasma lipids, promote macrophage infiltration into white adipose tissue. Diabetes. 2007 Mar; 56(3): 564-73. PMID: 17327423, PII: 56/3/564, DOI: 10.2337/db06-1375, ISSN: 0012-1797.

Hasty AH, Gruen ML, Terry ES, Surmi BK, Atkinson RD, Gao L, Morrow JD. Effects of vitamin E on oxidative stress and atherosclerosis in an obese hyperlipidemic mouse model. J. Nutr. Biochem [print-electronic]. 2007 Feb; 18(2): 127-33. PMID: 16781857, PII: S0955-2863(06)00095-7, DOI: 10.1016/j.jnutbio.2006.03.012, ISSN: 0955-2863.

Wang Y, Oeser JK, Yang C, Sarkar S, Hackl SI, Hasty AH, McGuinness OP, Paradee W, Hutton JC, Powell DR, O'Brien RM. Deletion of the gene encoding the ubiquitously expressed glucose-6-phosphatase catalytic subunit-related protein (UGRP)/glucose-6-phosphatase catalytic subunit-beta results in lowered plasma cholesterol and elevated glucagon. J. Biol. Chem [print-electronic]. 2006 Dec 12/29/2006; 281(52): 39982-9. PMID: 17023421, PII: M605858200, DOI: 10.1074/jbc.M605858200, ISSN: 0021-9258.

Kato T, Shimano H, Yamamoto T, Yokoo T, Endo Y, Ishikawa M, Matsuzaka T, Nakagawa Y, Kumadaki S, Yahagi N, Takahashi A, Sone H, Suzuki H, Toyoshima H, Hasty AH, Takahashi S, Gomi H, Izumi T, Yamada N. Granuphilin is activated by SREBP-1c and involved in impaired insulin secretion in diabetic mice. Cell Metab. 2006 Aug; 4(2): 143-54. PMID: 16890542, PII: S1550-4131(06)00239-7, DOI: 10.1016/j.cmet.2006.06.009, ISSN: 1550-4131.

Saraswathi V, Hasty AH. The role of lipolysis in mediating the proinflammatory effects of very low density lipoproteins in mouse peritoneal macrophages. J. Lipid Res [print-electronic]. 2006 Jul; 47(7): 1406-15. PMID: 16639077, PII: M600159-JLR200, DOI: 10.1194/jlr.M600159-JLR200, ISSN: 0022-2275.

Braun NA, Mohler PJ, Weisgraber KH, Hasty AH, Linton MF, Yancey PG, Su YR, Fazio S, Swift LL. Intracellular trafficking of recycling apolipoprotein E in Chinese hamster ovary cells. J. Lipid Res [print-electronic]. 2006 Jun; 47(6): 1176-86. PMID: 16534141, PII: M500503-JLR200, DOI: 10.1194/jlr.M500503-JLR200, ISSN: 0022-2275.

Nuotio-Antar AM, Hasty AH, Kovacs WJ. Quantitation and cellular localization of 11beta-HSD1 expression in murine thymus. J. Steroid Biochem. Mol. Biol [print-electronic]. 2006 May; 99(2-3): 93-9. PMID: 16621520, PII: S0960-0760(06)00066-5, DOI: 10.1016/j.jsbmb.2006.01.011, ISSN: 0960-0760.

Gruen ML, Saraswathi V, Nuotio-Antar AM, Plummer MR, Coenen KR, Hasty AH. Plasma insulin levels predict atherosclerotic lesion burden in obese hyperlipidemic mice. Atherosclerosis [print-electronic]. 2006 May; 186(1): 54-64. PMID: 16102772, PII: S0021-9150(05)00458-2, DOI: 10.1016/j.atherosclerosis.2005.07.007, ISSN: 0021-9150.

Liang X, Kanjanabuch T, Mao SL, Hao CM, Tang YW, Declerck PJ, Hasty AH, Wasserman DH, Fogo AB, Ma LJ. Plasminogen activator inhibitor-1 modulates adipocyte differentiation. Am. J. Physiol. Endocrinol. Metab [print-electronic]. 2006 Jan; 290(1): E103-E113. PMID: 16144810, PII: 00605.2004, DOI: 10.1152/ajpendo.00605.2004, ISSN: 0193-1849.

Nakagawa Y, Shimano H, Yoshikawa T, Ide T, Tamura M, Furusawa M, Yamamoto T, Inoue N, Matsuzaka T, Takahashi A, Hasty AH, Suzuki H, Sone H, Toyoshima H, Yahagi N, Yamada N. TFE3 transcriptionally activates hepatic IRS-2, participates in insulin signaling and ameliorates diabetes. Nat. Med [print-electronic]. 2006 Jan; 12(1): 107-13. PMID: 16327801, PII: nm1334, DOI: 10.1038/nm1334, ISSN: 1078-8956.

Zhu MY, Hasty AH, Harris C, Linton MF, Fazio S, Swift LL. Physiological relevance of apolipoprotein E recycling: studies in primary mouse hepatocytes. Metab. Clin. Exp. 2005 Oct; 54(10): 1309-15. PMID: 16154429, PII: S0026-0495(05)00181-2, DOI: 10.1016/j.metabol.2005.04.019, ISSN: 0026-0495.

Gruen ML, Plummer MR, Zhang W, Posey KA, Linton MF, Fazio S, Hasty AH. Persistence of high density lipoprotein particles in obese mice lacking apolipoprotein A-I. J. Lipid Res [print-electronic]. 2005 Sep; 46(9): 2007-14. PMID: 15995171, PII: M500181-JLR200, DOI: 10.1194/jlr.M500181-JLR200, ISSN: 0022-2275.

Bodary PF, Gu S, Shen Y, Hasty AH, Buckler JM, Eitzman DT. Recombinant leptin promotes atherosclerosis and thrombosis in apolipoprotein E-deficient mice. Arterioscler. Thromb. Vasc. Biol [print-electronic]. 2005 Aug; 25(8): e119-22. PMID: 15947243, PII:, DOI: 10.1161/, ISSN: 1524-4636.

Hasty AH, Plummer MR, Weisgraber KH, Linton MF, Fazio S, Swift LL. The recycling of apolipoprotein E in macrophages: influence of HDL and apolipoprotein A-I. J. Lipid Res [print-electronic]. 2005 Jul; 46(7): 1433-9. PMID: 15805547, PII: M400418-JLR200, DOI: 10.1194/jlr.M400418-JLR200, ISSN: 0022-2275.

Su YR, Dove DE, Major AS, Hasty AH, Boone B, Linton MF, Fazio S. Reduced ABCA1-mediated cholesterol efflux and accelerated atherosclerosis in apolipoprotein E-deficient mice lacking macrophage-derived ACAT1. Circulation [print-electronic]. 2005 May 5/10/2005; 111(18): 2373-81. PMID: 15851589, PII: 01.CIR.0000164236.19860.13, DOI: 10.1161/01.CIR.0000164236.19860.13, ISSN: 1524-4539.

Matsuzaka T, Shimano H, Yahagi N, Amemiya-Kudo M, Okazaki H, Tamura Y, Iizuka Y, Ohashi K, Tomita S, Sekiya M, Hasty A, Nakagawa Y, Sone H, Toyoshima H, Ishibashi S, Osuga J, Yamada N. Insulin-independent induction of sterol regulatory element-binding protein-1c expression in the livers of streptozotocin-treated mice. Diabetes. 2004 Mar; 53(3): 560-9. PMID: 14988238, ISSN: 0012-1797.

Su YR, Ishiguro H, Major AS, Dove DE, Zhang W, Hasty AH, Babaev VR, Linton MF, Fazio S. Macrophage apolipoprotein A-I expression protects against atherosclerosis in ApoE-deficient mice and up-regulates ABC transporters. Mol. Ther. 2003 Oct; 8(4): 576-83. PMID: 14529830, PII: S1525001603002144, ISSN: 1525-0016.

Yoshikawa T, Ide T, Shimano H, Yahagi N, Amemiya-Kudo M, Matsuzaka T, Yatoh S, Kitamine T, Okazaki H, Tamura Y, Sekiya M, Takahashi A, Hasty AH, Sato R, Sone H, Osuga J, Ishibashi S, Yamada N. Cross-talk between peroxisome proliferator-activated receptor (PPAR) alpha and liver X receptor (LXR) in nutritional regulation of fatty acid metabolism. I. PPARs suppress sterol regulatory element binding protein-1c promoter through inhibition of LXR signaling. Mol. Endocrinol [print-electronic]. 2003 Jul; 17(7): 1240-54. PMID: 12730331, PII: me.2002-0190, DOI: 10.1210/me.2002-0190, ISSN: 0888-8809.

Raffaï RL, Hasty AH, Wang Y, Mettler SE, Sanan DA, Linton MF, Fazio S, Weisgraber KH. Hepatocyte-derived ApoE is more effective than non-hepatocyte-derived ApoE in remnant lipoprotein clearance. J. Biol. Chem [print-electronic]. 2003 Mar 3/28/2003; 278(13): 11670-5. PMID: 12551940, PII: M212873200, DOI: 10.1074/jbc.M212873200, ISSN: 0021-9258.

Farkas MH, Swift LL, Hasty AH, Linton MF, Fazio S. The recycling of apolipoprotein E in primary cultures of mouse hepatocytes. Evidence for a physiologic connection to high density lipoprotein metabolism. J. Biol. Chem [print-electronic]. 2003 Mar 3/14/2003; 278(11): 9412-7. PMID: 12524433, PII: M208026200, DOI: 10.1074/jbc.M208026200, ISSN: 0021-9258.

Fazio S, Babaev VR, Burleigh ME, Major AS, Hasty AH, Linton MF. Physiological expression of macrophage apoE in the artery wall reduces atherosclerosis in severely hyperlipidemic mice. J. Lipid Res. 2002 Oct; 43(10): 1602-9. PMID: 12364544, ISSN: 0022-2275.

Amemiya-Kudo M, Shimano H, Hasty AH, Yahagi N, Yoshikawa T, Matsuzaka T, Okazaki H, Tamura Y, Iizuka Y, Ohashi K, Osuga J, Harada K, Gotoda T, Sato R, Kimura S, Ishibashi S, Yamada N. Transcriptional activities of nuclear SREBP-1a, -1c, and -2 to different target promoters of lipogenic and cholesterogenic genes. J. Lipid Res. 2002 Aug; 43(8): 1220-35. PMID: 12177166, ISSN: 0022-2275.

Matsuzaka T, Shimano H, Yahagi N, Yoshikawa T, Amemiya-Kudo M, Hasty AH, Okazaki H, Tamura Y, Iizuka Y, Ohashi K, Osuga J, Takahashi A, Yato S, Sone H, Ishibashi S, Yamada N. Cloning and characterization of a mammalian fatty acyl-CoA elongase as a lipogenic enzyme regulated by SREBPs. J. Lipid Res. 2002 Jun; 43(6): 911-20. PMID: 12032166, ISSN: 0022-2275.

Yahagi N, Shimano H, Hasty AH, Matsuzaka T, Ide T, Yoshikawa T, Amemiya-Kudo M, Tomita S, Okazaki H, Tamura Y, Iizuka Y, Ohashi K, Osuga J, Harada K, Gotoda T, Nagai R, Ishibashi S, Yamada N. Absence of sterol regulatory element-binding protein-1 (SREBP-1) ameliorates fatty livers but not obesity or insulin resistance in Lep(ob)/Lep(ob) mice. J. Biol. Chem [print-electronic]. 2002 May 5/31/2002; 277(22): 19353-7. PMID: 11923308, PII: M201584200, DOI: 10.1074/jbc.M201584200, ISSN: 0021-9258.

Matsuzaka T, Shimano H, Yahagi N, Amemiya-Kudo M, Yoshikawa T, Hasty AH, Tamura Y, Osuga J, Okazaki H, Iizuka Y, Takahashi A, Sone H, Gotoda T, Ishibashi S, Yamada N. Dual regulation of mouse Delta(5)- and Delta(6)-desaturase gene expression by SREBP-1 and PPARalpha. J. Lipid Res. 2002 Jan; 43(1): 107-14. PMID: 11792729, ISSN: 0022-2275.

Yoshida H, Hasty AH, Major AS, Ishiguro H, Su YR, Gleaves LA, Babaev VR, Linton MF, Fazio S. Isoform-specific effects of apolipoprotein E on atherogenesis: gene transduction studies in mice. Circulation. 2001 Dec 12/4/2001; 104(23): 2820-5. PMID: 11733401, ISSN: 1524-4539.

Hasty AH, Shimano H, Osuga J, Namatame I, Takahashi A, Yahagi N, Perrey S, Iizuka Y, Tamura Y, Amemiya-Kudo M, Yoshikawa T, Okazaki H, Ohashi K, Harada K, Matsuzaka T, Sone H, Gotoda T, Nagai R, Ishibashi S, Yamada N. Severe hypercholesterolemia, hypertriglyceridemia, and atherosclerosis in mice lacking both leptin and the low density lipoprotein receptor. J. Biol. Chem [print-electronic]. 2001 Oct 10/5/2001; 276(40): 37402-8. PMID: 11445560, PII: M010176200, DOI: 10.1074/jbc.M010176200, ISSN: 0021-9258.

Yoshikawa T, Shimano H, Amemiya-Kudo M, Yahagi N, Hasty AH, Matsuzaka T, Okazaki H, Tamura Y, Iizuka Y, Ohashi K, Osuga J, Harada K, Gotoda T, Kimura S, Ishibashi S, Yamada N. Identification of liver X receptor-retinoid X receptor as an activator of the sterol regulatory element-binding protein 1c gene promoter. Mol. Cell. Biol. 2001 May; 21(9): 2991-3000. PMID: 11287605, PMCID: PMC86928, DOI: 10.1128/MCB.21.9.2991-3000.2001, ISSN: 0270-7306.

Amemiya-Kudo M, Shimano H, Yoshikawa T, Yahagi N, Hasty AH, Okazaki H, Tamura Y, Shionoiri F, Iizuka Y, Ohashi K, Osuga J, Harada K, Gotoda T, Sato R, Kimura S, Ishibashi S, Yamada N. Promoter analysis of the mouse sterol regulatory element-binding protein-1c gene. J. Biol. Chem. 2000 Oct 10/6/2000; 275(40): 31078-85. PMID: 10918064, PII: M005353200, DOI: 10.1074/jbc.M005353200, ISSN: 0021-9258.

Hasty AH, Shimano H, Yahagi N, Amemiya-Kudo M, Perrey S, Yoshikawa T, Osuga J, Okazaki H, Tamura Y, Iizuka Y, Shionoiri F, Ohashi K, Harada K, Gotoda T, Nagai R, Ishibashi S, Yamada N. Sterol regulatory element-binding protein-1 is regulated by glucose at the transcriptional level. J. Biol. Chem. 2000 Oct 10/6/2000; 275(40): 31069-77. PMID: 10913129, PII: M003335200, DOI: 10.1074/jbc.M003335200, ISSN: 0021-9258.

Yahagi N, Shimano H, Hasty AH, Amemiya-Kudo M, Okazaki H, Tamura Y, Iizuka Y, Shionoiri F, Ohashi K, Osuga J, Harada K, Gotoda T, Nagai R, Ishibashi S, Yamada N. A crucial role of sterol regulatory element-binding protein-1 in the regulation of lipogenic gene expression by polyunsaturated fatty acids. J. Biol. Chem. 1999 Dec 12/10/1999; 274(50): 35840-4. PMID: 10585468, ISSN: 0021-9258.

Shimano H, Yahagi N, Amemiya-Kudo M, Hasty AH, Osuga J, Tamura Y, Shionoiri F, Iizuka Y, Ohashi K, Harada K, Gotoda T, Ishibashi S, Yamada N. Sterol regulatory element-binding protein-1 as a key transcription factor for nutritional induction of lipogenic enzyme genes. J. Biol. Chem. 1999 Dec 12/10/1999; 274(50): 35832-9. PMID: 10585467, ISSN: 0021-9258.

Hasty AH, Linton MF, Swift LL, Fazio S. Determination of the lower threshold of apolipoprotein E resulting in remnant lipoprotein clearance. J. Lipid Res. 1999 Aug; 40(8): 1529-38. PMID: 10428991, ISSN: 0022-2275.

Hasty AH, Linton MF, Brandt SJ, Babaev VR, Gleaves LA, Fazio S. Retroviral gene therapy in ApoE-deficient mice: ApoE expression in the artery wall reduces early foam cell lesion formation. Circulation. 1999 May 5/18/1999; 99(19): 2571-6. PMID: 10330390, ISSN: 0009-7322.

Fazio S, Linton MF, Hasty AH, Swift LL. Recycling of apolipoprotein E in mouse liver. J. Biol. Chem. 1999 Mar 3/19/1999; 274(12): 8247-53. PMID: 10075730, ISSN: 0021-9258.

Linton MF, Hasty AH, Babaev VR, Fazio S. Hepatic apo E expression is required for remnant lipoprotein clearance in the absence of the low density lipoprotein receptor. J. Clin. Invest. 1998 Apr 4/15/1998; 101(8): 1726-36. PMID: 9541504, PMCID: PMC508755, DOI: 10.1172/JCI2181, ISSN: 0021-9738.

Fazio S, Babaev VR, Murray AB, Hasty AH, Carter KJ, Gleaves LA, Atkinson JB, Linton MF. Increased atherosclerosis in mice reconstituted with apolipoprotein E null macrophages. Proc. Natl. Acad. Sci. U.S.A. 1997 Apr 4/29/1997; 94(9): 4647-52. PMID: 9114045, PMCID: PMC20778, ISSN: 0027-8424.

Fazio S, Hasty AH, Carter KJ, Murray AB, Price JO, Linton MF. Leukocyte low density lipoprotein receptor (LDL-R) does not contribute to LDL clearance in vivo: bone marrow transplantation studies in the mouse. J. Lipid Res. 1997 Feb; 38(2): 391-400. PMID: 9162757, ISSN: 0022-2275.

Available Postdoctoral Position Details
Posted: 9/24/2015
The overarching goal of the Hasty Laboratory is to determine mechanisms by which obesity increases risk of metabolic disease. We are focused primarily on the role that resident and recruited immune cells play in adipose tissue function in the lean and obese states. We are seeking a highly motivated, energetic, creative thinking person to join our team as a postdoctoral fellow. Candidates with experience in general immunological techniques such as flow cytometry and/or familiarity with cellular iron handling will be given special consideration. Please contact Alyssa Hasty (, providing your CV and contact information for individuals who will provide reference letters.