G. Roger Chalkley, D.Phil.



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Faculty Appointments
Senior Associate Dean for Biomedical Research, Education and Training Professor of Molecular Physiology and Biophysics Professor of Medical Education and Administration
D.Phil., University of Oxford, Oxford, United KingdomM.A., University of Oxford, Oxford, United KingdomB.A., University of Oxford, Oxford, United Kingdom
Office Address
Biomedical Research Education and Training
340 Light Hall
Vanderbilt University
Nashville, TN 37232-0301
Chalkley RG. International students at Vanderbilt [letter]. Science. 2004 Jul 7/30/2004; 305(5684): 607-9. PMID: 15286345, PII: 305/5684/607d, DOI: 10.1126/science.305.5684.607d, ISSN: 1095-9203.

Budhiraja R, Kayyali US, Karamsetty M, Fogel M, Hill NS, Chalkley R, Finlay GA, Hassoun PM. Estrogen modulates xanthine dehydrogenase/xanthine oxidase activity by a receptor-independent mechanism. Antioxid. Redox Signal. 2003 Dec; 5(6): 705-11. PMID: 14588143, DOI: 10.1089/152308603770380007, ISSN: 1523-0864.

Kayyali US, Budhiraja R, Pennella CM, Cooray S, Lanzillo JJ, Chalkley R, Hassoun PM. Upregulation of xanthine oxidase by tobacco smoke condensate in pulmonary endothelial cells. Toxicol. Appl. Pharmacol. 2003 Apr 4/1/2003; 188(1): 59-68. PMID: 12668123, PII: S0041008X02000765, ISSN: 0041-008X.

She P, Shiota M, Shelton KD, Chalkley R, Postic C, Magnuson MA. Phosphoenolpyruvate carboxykinase is necessary for the integration of hepatic energy metabolism. Mol. Cell. Biol. 2000 Sep; 20(17): 6508-17. PMID: 10938127, PMCID: PMC86125, ISSN: 0270-7306.

Cissell MA, Chalkley R. Characterization of a kidney-specific pattern of chromatin structure in the rat phosphoenolpyruvate carboxykinase gene. Biochim. Biophys. Acta. 1999 Jun 6/9/1999; 1445(3): 299-313. PMID: 10366713, PII: S0167-4781(99)00049-4, ISSN: 0006-3002.

Nagvekar N, Corlett L, Jacobson LW, Matsuo H, Chalkley R, Driscoll PC, Deshpande S, Spack EG, Willcox N. Scanning a DRB3*0101 (DR52a)-restricted epitope cross-presented by DR3: overlapping natural and artificial determinants in the human acetylcholine receptor. J. Immunol. 1999 Apr 4/1/1999; 162(7): 4079-87. PMID: 10201931, ISSN: 0022-1767.

Williams CP, Postic C, Robin D, Robin P, Parrinello J, Shelton K, Printz RL, Magnuson MA, Granner DK, Forest C, Chalkley R. Isolation and characterization of the mouse cytosolic phosphoenolpyruvate carboxykinase (GTP) gene: evidence for tissue-specific hypersensitive sites. Mol. Cell. Endocrinol. 1999 Feb 2/25/1999; 148(1-2): 67-77. PMID: 10221772, PII: S0303-7207(98)00234-2, ISSN: 0303-7207.

Clark MP, Chow CW, Rinaldo JE, Chalkley R. Multiple domains for initiator binding proteins TFII-I and YY-1 are present in the initiator and upstream regions of the rat XDH/XO TATA-less promoter. Nucleic Acids Res. 1998 Jun 6/1/1998; 26(11): 2813-20. PMID: 9592172, PMCID: PMC147594, PII: gkb444, ISSN: 0305-1048.

Clark MP, Chow CW, Rinaldo JE, Chalkley R. Correct usage of multiple transcription initiation sites and C/EBP-dependent transcription activation of the rat XDH/XO TATA-less promoter requires downstream elements located in the coding region of the gene. Nucleic Acids Res. 1998 Apr 4/1/1998; 26(7): 1801-6. PMID: 9512555, PMCID: PMC147445, PII: gkb316, ISSN: 0305-1048.

Chow CW, Clark MP, Rinaldo JE, Chalkley R. In vitro transcription in the presence of DNA oligonucleotides can generate strong anomalous initiation sites. Anal. Biochem. 1996 Mar 3/1/1996; 235(1): 82-8. PMID: 8850550, PII: S0003-2697(96)90094-9, DOI: 10.1006/abio.1996.0094, ISSN: 0003-2697.

Williams CP, Granner DK, Magnuson MA, Chalkley R. Cell specific differences in DNase I hypersensitivity between the two promoters of the rat glucokinase gene. Biochem. Biophys. Res. Commun. 1995 Oct 10/4/1995; 215(1): 272-9. PMID: 7575601, PII: S0006-291X(85)72462-X, DOI: 10.1006/bbrc.1995.2462, ISSN: 0006-291X.

Chow CW, Clark MP, Rinaldo JE, Chalkley R. Multiple initiators and C/EBP binding sites are involved in transcription from the TATA-less rat XDH/XO basal promoter. Nucleic Acids Res. 1995 Aug 8/25/1995; 23(16): 3132-40. PMID: 7667089, PMCID: PMC307170, PII: 5e0321, ISSN: 0305-1048.

Svaren J, Wineinger BD, Chalkley R. Extent of in vivo binding by an upstream activation factor and the role of multiple binding sites in synergistic transcriptional activation. J. Biol. Chem. 1994 Aug 8/12/1994; 269(32): 20771-9. PMID: 8051179, ISSN: 0021-9258.

Chow CW, Clark M, Rinaldo J, Chalkley R. Identification of the rat xanthine dehydrogenase/oxidase promoter. Nucleic Acids Res. 1994 May 5/25/1994; 22(10): 1846-54. PMID: 8208609, PMCID: PMC308083, ISSN: 0305-1048.

Svaren J, Klebanow E, Sealy L, Chalkley R. Analysis of the competition between nucleosome formation and transcription factor binding. J. Biol. Chem. 1994 Mar 3/25/1994; 269(12): 9335-44. PMID: 8132673, ISSN: 0021-9258.

Svaren J and Chalkley, R. Binding of Histones and IBF are Mutually Exclusive and DNA Length Dependent: Implications for a Kinetic Window During Chromatin Assembly. J. Biol. Chem. 1994; 263: 14044-52.

Svaren, J., Klebanow, E., Sealy, L., and Chalkley, R. Binding of Histones and IBF are mutually exclusive and DNA length dependent: Implications for a Kinetic Window during Chromatin Assembly. J. Biol. Chem. 1994; 269: 9335-44.

Wineinger, B., Svaren, J., and Chalkley, R. The Interplay of Chromatin Structure and Transcription Factor Binding in the Synergistic Activation of a Yeast UAS. J. Biol. Chem. 1994; 269: 20771-9.

Faber S, O'Brien RM, Imai E, Granner DK, Chalkley R. Dynamic aspects of DNA/protein interactions in the transcriptional initiation complex and the hormone-responsive domains of the phosphoenolpyruvate carboxykinase promoter in vivo. J. Biol. Chem. 1993 Nov 11/25/1993; 268(33): 24976-85. PMID: 8227059, ISSN: 0021-9258.

Ozer J, Chalkley R, Sealy L. Characterization of rat pseudogenes for enhancer factor I subunit A: ripping provides clues to the evolution of the EFIA/dbpB/YB-1 multigene family. Gene. 1993 Nov 11/15/1993; 133(2): 187-95. PMID: 8224907, ISSN: 0378-1119.

Ozer J, Chalkley R, Sealy L. Isolation of the CCAAT transcription factor subunit EFIA cDNA and a potentially functional EFIA processed pseudogene from Bos taurus: insights into the evolution of the EFIA/dbpB/YB-1 gene family. Gene. 1993 Feb 2/28/1993; 124(2): 223-30. PMID: 8444345, ISSN: 0378-1119.

Ozer J., Chalkley, R. and Sealy, L. The Characterization of the Rat CCAAT Transcription Factor EFIA Processed Pseudogenes: Novel Retroposons present clues to the Evolution of the EFIA/dbpB/Yb-1 Gene Family. 124. 1993; Gene: 491-8.

Cheyette TE, Ip T, Faber S, Matsui Y, Chalkley R. Characterization of the factors binding to a PEPCK gene upstream hypersensitive site with LCR activity. Nucleic Acids Res. 1992 Jul 7/11/1992; 20(13): 3427-33. PMID: 1385863, PMCID: PMC312499, ISSN: 0305-1048.

Faber S, Ip T, Granner D, Chalkley R. The interplay of ubiquitous DNA-binding factors, availability of binding sites in the chromatin, and DNA methylation in the differential regulation of phosphoenolpyruvate carboxykinase gene expression. Nucleic Acids Res. 1991 Sep 9/11/1991; 19(17): 4681-8. PMID: 1716357, PMCID: PMC328709, ISSN: 0305-1048.

Ozer J, Faber M, Chalkley R, Sealy L. Isolation and characterization of a cDNA clone for the CCAAT transcription factor EFIA reveals a novel structural motif. J. Biol. Chem. 1990 Dec 12/25/1990; 265(36): 22143-52. PMID: 1967130, ISSN: 0021-9258.

Ip YT, Fournier RE, Chalkley R. Extinction of phosphoenolpyruvate carboxykinase gene expression is associated with loss of a specific chromatin-binding protein from a far upstream domain. Mol. Cell. Biol. 1990 Jul; 10(7): 3782-7. PMID: 2355923, PMCID: PMC360833, ISSN: 0270-7306.

Ip YT, Poon D, Stone D, Granner DK, Chalkley R. Interaction of a liver-specific factor with an enhancer 4.8 kilobases upstream of the phosphoenolpyruvate carboxykinase gene. Mol. Cell. Biol. 1990 Jul; 10(7): 3770-81. PMID: 2355922, PMCID: PMC360831, ISSN: 0270-7306.

Felts SJ, Weil PA, Chalkley R. Transcription factor requirements for in vitro formation of transcriptionally competent 5S rRNA gene chromatin. Mol. Cell. Biol. 1990 May; 10(5): 2390-401. PMID: 2183033, PMCID: PMC360587, ISSN: 0270-7306.

Karnitz L, Poon D, Weil PA, Chalkley R. Identification and purification of a yeast transcriptional trans-activator. The yeast homolog of the Rous sarcoma virus internal enhancer binding factor. J. Biol. Chem. 1990 Apr 4/15/1990; 265(11): 6131-8. PMID: 2156843, ISSN: 0021-9258.

Svaren J, Chalkley R. The structure and assembly of active chromatin. Trends Genet. 1990 Feb; 6(2): 52-6. PMID: 2186529, ISSN: 0168-9525.

Fournier, K. E. and Chalkley, R. Extinction Of PEPCK Gene Expression is associated with Loss of a Specific chromatin-Binding protein from a Far upstream Region. Mol. Cell. Biol. 1990; 10: 3782-7.

Karnitz L, Poon D, Weil PA, Chalkley R. Purification and properties of the Rous sarcoma virus internal enhancer binding factor. Mol. Cell. Biol. 1989 May; 9(5): 1929-39. PMID: 2546054, PMCID: PMC362984, ISSN: 0270-7306.

Ip YT, Granner DK, Chalkley R. Hormonal regulation of phosphoenolpyruvate carboxykinase gene expression is mediated through modulation of an already disrupted chromatin structure. Mol. Cell. Biol. 1989 Mar; 9(3): 1289-97. PMID: 2657389, PMCID: PMC362721, ISSN: 0270-7306.

Sealy L, Burgess RR, Cotten M, Chalkley R. Purification of Xenopus egg nucleoplasmin and its use in chromatin assembly in vitro. Meth. Enzymol. 1989; 170: 612-30. PMID: 2549340, PII: 0076-6879(89)70068-9, ISSN: 0076-6879.

Granner, D.K. and Chalkley, R. Hormonal Regulation of PEPCK Gene Expression is Regulated through Modulation of an Already Disrupted Chromatin Structure. Mol. Cell. Biol. 1989; 9: 1289-97.

Felts, S., Weil, T. and Chalkley, R. Transcription Factor Requirements For Reassembly and Transcription of Yeast 5-S Chromatin Templates In Vitro and In Vivo. Mol. Cell. Biol. 1989; 10: 2390-401.

Ip YT, Jackson V, Meier J, Chalkley R. The separation of transcriptionally engaged genes. J. Biol. Chem. 1988 Oct 10/5/1988; 263(28): 14044-52. PMID: 3170538, ISSN: 0021-9258.

T.Y., Jackson, V., Meier, J. and Chalkley, R. The Separation of Transcriptionally Engaged Genes. J. Biol. Chem. 1988; 263: 14044-52.

Cotten M, Chalkley R. Purification of a novel, nucleoplasmin-like protein from somatic nuclei. EMBO J. 1987 Dec 12/20/1987; 6(13): 3945-54. PMID: 3443097, PMCID: PMC553873, ISSN: 0261-4189.

Karnitz L, Faber S, Chalkley R. Specific nuclear proteins interact with the Rous sarcoma virus internal enhancer and share a common element with the enhancer located in the long terminal repeat of the virus. Nucleic Acids Res. 1987 Dec 12/10/1987; 15(23): 9841-59. PMID: 2827114, PMCID: PMC306535, ISSN: 0305-1048.

Svaren J, Inagami S, Lovegren E, Chalkley R. DNA denatures upon drying after ethanol precipitation. Nucleic Acids Res. 1987 Nov 11/11/1987; 15(21): 8739-54. PMID: 3684571, PMCID: PMC306402, ISSN: 0305-1048.

Moseley PL, Chalkley R. Bleomycin-induced DNA cleavage: studies in vitro and in intact cells. J. Lab. Clin. Med. 1987 Nov; 110(5): 618-23. PMID: 2444664, PII: 0022-2143(87)90454-9, ISSN: 0022-2143.

Felts SJ, Weil PA, Chalkley R. Novobiocin inhibits interactions required for yeast TFIIIB sequestration during stable transcription complex formation in vitro. Nucleic Acids Res. 1987 Feb 2/25/1987; 15(4): 1493-506. PMID: 3547336, PMCID: PMC340563, ISSN: 0305-1048.

Sealey L, Chalkley R. At least two nuclear proteins bind specifically to the Rous sarcoma virus long terminal repeat enhancer. Mol. Cell. Biol. 1987 Feb; 7(2): 787-98. PMID: 3029568, PMCID: PMC365136, ISSN: 0270-7306.

Chalkley, R., Cotten, M., and Sealy, L. The Role of Phosphorylation in the Activation for Chromatin Assembly of Xenopus Nucleoplasmin. Transcriptional Control of Gene. 1987; 247-58.

Sealy L, Cotten M, Chalkley R. Novobiocin inhibits passive chromatin assembly in vitro. EMBO J. 1986 Dec 12/1/1986; 5(12): 3305-11. PMID: 3028779, PMCID: PMC1167327, ISSN: 0261-4189.

Cotten M, Sealy L, Chalkley R. Massive phosphorylation distinguishes Xenopus laevis nucleoplasmin isolated from oocytes or unfertilized eggs. Biochemistry. 1986 Sep 9/9/1986; 25(18): 5063-9. PMID: 3768332, ISSN: 0006-2960.

Wright PS, Dudley DT, Chalkley R. Butyrate effects on normal and adapted hepatoma cells: morphological response and implications for vectoral cholesterol transport. Arch. Biochem. Biophys. 1986 Jul; 248(1): 243-52. PMID: 3729417, PII: 0003-9861(86)90422-4, ISSN: 0003-9861.

Sealy L, Cotten M, Chalkley R. Xenopus nucleoplasmin: egg vs. oocyte. Biochemistry. 1986 May 5/20/1986; 25(10): 3064-72. PMID: 3521726, ISSN: 0006-2960.

Cotten M, Bresnahan D, Thompson S, Sealy L, Chalkley R. Novobiocin precipitates histones at concentrations normally used to inhibit eukaryotic type II topoisomerase. Nucleic Acids Res. 1986 May 5/12/1986; 14(9): 3671-86. PMID: 3714493, PMCID: PMC339807, ISSN: 0305-1048.

Strauch AR, Offord JD, Chalkley R, Rubenstein PA. Characterization of actin mRNA levels during BC3H1 cell differentiation. J. Biol. Chem. 1986 Jan 1/15/1986; 261(2): 849-55. PMID: 3941103, ISSN: 0021-9258.

Jackson V, Chalkley R. Histone segregation on replicating chromatin. Biochemistry. 1985 Nov 11/19/1985; 24(24): 6930-8. PMID: 3935168, ISSN: 0006-2960.

Jackson V, Chalkley R. Histone synthesis and deposition in the G1 and S phases of hepatoma tissue culture cells. Biochemistry. 1985 Nov 11/19/1985; 24(24): 6921-30. PMID: 3935167, ISSN: 0006-2960.

Chalkley R, Shires A. The isolation of HTC variant cells which can replicate in butyrate. Changes in histone acetylation and tyrosine aminotransferase induction. J. Biol. Chem. 1985 Jun 6/25/1985; 260(12): 7698-704. PMID: 2860113, ISSN: 0021-9258.

Louters L, Chalkley R. Exchange of histones H1, H2A, and H2B in vivo. Biochemistry. 1985 Jun 6/18/1985; 24(13): 3080-5. PMID: 4027229, ISSN: 0006-2960.

Cotten M, Chalkley R. Hyperacetylated histones facilitate chromatin assembly in vitro. Nucleic Acids Res. 1985 Jan 1/25/1985; 13(2): 401-14. PMID: 2582355, PMCID: PMC341004, ISSN: 0305-1048.

Smith PA, Jackson V, Chalkley R. Two-stage maturation process for newly replicated chromatin. Biochemistry. 1984 Mar 3/27/1984; 23(7): 1576-81. PMID: 6722110, ISSN: 0006-2960.

Louters L, Chalkley R. In vitro exchange of nucleosomal histones H2a and H2b. Biochemistry. 1984 Jan 1/31/1984; 23(3): 547-52. PMID: 6322843, ISSN: 0006-2960.

Plesko MM, Hargrove JL, Granner DK, Chalkley R. Inhibition by sodium butyrate of enzyme induction by glucocorticoids and dibutyryl cyclic AMP. A role for the rapid form of histone acetylation. J. Biol. Chem. 1983 Nov 11/25/1983; 258(22): 13738-44. PMID: 6196355, ISSN: 0021-9258.

Covault J, Perry M, Chalkley R. Effects of histone hyperacetylation and hypoacetylation on RNA synthesis in HTC cells. J. Biol. Chem. 1982 Nov 11/25/1982; 257(22): 13433-40. PMID: 7142157, ISSN: 0021-9258.

Perry M, Chalkley R. Histone acetylation increases the solubility of chromatin and occurs sequentially over most of the chromatin. A novel model for the biological role of histone acetylation. J. Biol. Chem. 1982 Jul 7/10/1982; 257(13): 7336-47. PMID: 7085629, ISSN: 0021-9258.

Covault J, Sealy L, Schnell R, Shires A, Chalkley R. Histone hypoacetylation following release of HTC cells from butyrate. J. Biol. Chem. 1982 May 5/25/1982; 257(10): 5809-15. PMID: 6950936, ISSN: 0021-9258.

Brotherton TW, Covault J, Shires A, Chalkley R. Only a small fraction of avian erythrocyte histone is involved in ongoing acetylation. Nucleic Acids Res. 1981 Oct 10/10/1981; 9(19): 5061-73. PMID: 7312625, PMCID: PMC327499, ISSN: 0305-1048.

Jackson V, Chalkley R. Use of whole-cell fixation to visualize replicating and maturing simian virus 40: identification of new viral gene product. Proc. Natl. Acad. Sci. U.S.A. 1981 Oct; 78(10): 6081-5. PMID: 6273846, PMCID: PMC348981, ISSN: 0027-8424.

Jackson V, Marshall S, Chalkley R. The sites of deposition of newly synthesized histone. Nucleic Acids Res. 1981 Sep 9/25/1981; 9(18): 4563-81. PMID: 7301583, PMCID: PMC327459, ISSN: 0305-1048.

Jackson V, Chalkley R. A reevaluation of new histone deposition on replicating chromatin. J. Biol. Chem. 1981 May 5/25/1981; 256(10): 5095-103. PMID: 6262316, ISSN: 0021-9258.

Perry M, Chalkley R. The effect of histone hyperacetylation on the nuclease sensitivity and the solubility of chromatin. J. Biol. Chem. 1981 Apr 4/10/1981; 256(7): 3313-8. PMID: 6259161, ISSN: 0021-9258.

Sealy L, Hartley J, Donelson J, Chalkley R, Hutchison N, Hamkalo B. Characterization of a highly repetitive sequence DNA family in rat. J. Mol. Biol. 1981 Jan 1/15/1981; 145(2): 291-318. PMID: 6167723, PII: 0022-2836(81)90207-2, ISSN: 0022-2836.

Bloxham DP, Chalkley RA, Cooper G. Chloromethyl ketone derivatives of fatty acids. Meth. Enzymol. 1981; 72: 592-604. PMID: 7311856, ISSN: 0076-6879.

Jackson V, Chalkley R. A new method for the isolation of replicative chromatin: selective deposition of histone on both new and old DNA. Cell. 1981 Jan; 23(1): 121-34. PMID: 7194149, PII: 0092-8674(81)90277-4, ISSN: 0092-8674.

Shoemaker CB, Chalkley R. H3-specific nucleohistone kinase of bovine thymus chromatin. Purification, characterization, and specificity for threonine residue 3. J. Biol. Chem. 1980 Nov 11/25/1980; 255(22): 11048-55. PMID: 6253499, ISSN: 0021-9258.

Covault J, Chalkley R. The identification of distinct populations of acetylated histone. J. Biol. Chem. 1980 Oct 10/10/1980; 255(19): 9110-6. PMID: 7410415, ISSN: 0021-9258.

Nelson D, Covault J, Chalkley R. Segregation of rapidly acetylated histones into a chromatin fraction released from intact nuclei by the action of micrococcal nuclease. Nucleic Acids Res. 1980 Apr 4/25/1980; 8(8): 1745-63. PMID: 7433128, PMCID: PMC324033, ISSN: 0305-1048.

Sealy L, Chalkley R. Modification of histones immediately following synthesis. Arch. Biochem. Biophys. 1979 Oct 10/1/1979; 197(1): 78-82. PMID: 543725, PII: 0003-9861(79)90221-2, ISSN: 0003-9861.

Gaubatz J, Ellis M, Chalkley R. Nuclease digestion studies of mouse chromatin as a function of age. J Gerontol. 1979 Sep; 34(5): 672-9. PMID: 469185, ISSN: 0022-1422.

Rubenstein P, Sealy L, Marshall S, Chalkley R. Cellular protein synthesis and inhibition of cell division are independent of butyrate-induced histone hyperacetylation. Nature. 1979 Aug 8/23/1979; 280(5724): 692-3. PMID: 471049, ISSN: 0028-0836.

Gaubatz J, Ellis M, Chalkley R. The structural organization of mouse chromatin as a function of age. Fed. Proc. 1979 May; 38(6): 1973-8. PMID: 437140, ISSN: 0014-9446.

Perry M, Nelson D, Moore M, Chalkley R. Histone deacetylation in nuclei isolated from hepatoma tissue culture cells. Inhibition by sodium butyrate. Biochim. Biophys. Acta. 1979 Feb 2/27/1979; 561(2): 517-25. PMID: 427171, ISSN: 0006-3002.

Nelson D, Perry ME, Chalkley R. A correlation between nucleosome spacer region susceptibility to DNase I and histone acetylation. Nucleic Acids Res. 1979 Feb; 6(2): 561-74. PMID: 424305, PMCID: PMC327713, ISSN: 0305-1048.

Moore M, Jackson V, Sealy L, Chalkley R. Comparative studies on highly metabolically active histone acetylation. Biochim. Biophys. Acta. 1979 Jan 1/26/1979; 561(1): 248-60. PMID: 105758, ISSN: 0006-3002.

Bloxham DP, Chalkley RA, Coghlin SJ, Salam W. Synthesis of chloromethyl ketone derivatives of fatty acids. Their use as specific inhibitors of acetoacetyl-coenzyme A thiolase, cholesterol biosynthesis and fatty acid synthesis. Biochem. J. 1978 Dec 12/1/1978; 175(3): 999-1011. PMID: 33667, PMCID: PMC1186163, ISSN: 0264-6021.

Nadeau P, Oliver DR, Chalkley R. Effect of inhibition of DNA synthesis on histone synthesis and deposition. Biochemistry. 1978 Nov 11/14/1978; 17(23): 4885-93. PMID: 718863, ISSN: 0006-2960.

Shoemaker CB, Chalkley R. An H3 histone-specific kinase isolated from bovine thymus chromatin. J. Biol. Chem. 1978 Aug 8/25/1978; 253(16): 5802-7. PMID: 209051, ISSN: 0021-9258.

Nelson DA, Perry M, Sealy L, Chalkley R. DNAse I preferentially digests chromatin containing hyperacetylated histones. Biochem. Biophys. Res. Commun. 1978 Jun 6/29/1978; 82(4): 1346-53. PMID: 697799, PII: 0006-291X(78)90337-6, ISSN: 0006-291X.

Sealy L, Chalkley R. DNA associated with hyperacetylated histone is preferentially digested by DNase I. Nucleic Acids Res. 1978 Jun; 5(6): 1863-76. PMID: 673837, PMCID: PMC342130, ISSN: 0305-1048.

Nelson DA, Perry WM, Chalkley R. Sensitivity of regions of chromatin containing hyperacetylated histones to DNAse I. Biochem. Biophys. Res. Commun. 1978 May 5/15/1978; 82(1): 365-63. PMID: 208559, ISSN: 0006-291X.

Sealy L, Chalkley R. The effect of sodium butyrate on histone modification. Cell. 1978 May; 14(1): 115-21. PMID: 667928, PII: 0092-8674(78)90306-9, ISSN: 0092-8674.

Hoffmann P, Chalkley R. Procedures for minimizing protease activity during isolation of nuclei, chromatin, and the histones. Methods Cell Biol. 1978; 17: 1-12. PMID: 703608, ISSN: 0091-679X.

Gaubatz J, Hardison R, Murphy J, Eichner ME, Chalkley R. The role of H1 in the structure of chromatin. Cold Spring Harb. Symp. Quant. Biol. 1978; 42 Pt 1: 265-71. PMID: 277345, ISSN: 0091-7451.

Hardison R, Chalkley R. Polyacrylamide gel electrophoretic fractionation of histones. Methods Cell Biol. 1978; 17: 235-51. PMID: 30021, ISSN: 0091-679X.

Gaubatz JW, Chalkley R. Distribution of H1 histone in chromatin digested by micrococcal nuclease. Nucleic Acids Res. 1977 Oct; 4(10): 3281-301. PMID: 928061, PMCID: PMC342654, ISSN: 0305-1048.

Hardison RC, Zeitler DP, Murphy JM, Chalkley R. Histone neighbors in nuclei and extended chromatin. Cell. 1977 Oct; 12(2): 417-27. PMID: 912751, PII: 0092-8674(77)90118-0, ISSN: 0092-8674.

Hoffmann PJ, Chalkley R. A neutral pH acrylamide gel electrophoretic system for histones and other basic proteins. Anal. Biochem. 1976 Dec; 76(2): 539-46. PMID: 11711, ISSN: 0003-2697.

Bloxham DP, Chalkley RA. Selective modification of rabbit muscle pyruvate kinase by 5-chloro-4-oxopentanoic acid. Biochem. J. 1976 Nov; 159(2): 201-11. PMID: 999648, PMCID: PMC1164107, ISSN: 0264-6021.

Chalkley RA, Bloxham DP. Identification of cysteine as the reactive group in pyruvate kinase alkylated by 5-chloro-4-oxopentanoic acid. Biochem. J. 1976 Nov; 159(2): 213-9. PMID: 187171, PMCID: PMC1164108, ISSN: 0264-6021.

Shires A, Carpenter MP, Chalkley R. A cysteine-containing H2B-like histone found in mature mammalian testis. J. Biol. Chem. 1976 Jul 7/10/1976; 251(13): 4155-8. PMID: 932025, ISSN: 0021-9258.

Jackson V, Granner D, Chalkley R. Deposition of histone onto the replicating chromosome: newly synthesized histone is not found near the replication fork. Proc. Natl. Acad. Sci. U.S.A. 1976 Jul; 73(7): 2266-9. PMID: 1065875, PMCID: PMC430523, ISSN: 0027-8424.

Jackson V, Shires A, Tanphaichitr N, Chalkley R. Modifications to histones immediately after synthesis. J. Mol. Biol. 1976 Jun 6/25/1976; 104(2): 471-83. PMID: 950668, PII: 0022-2836(76)90282-5, ISSN: 0022-2836.

Tanphaichitr N, Chalkley R. Production of high levels of phosphorylated F1 histone by zinc chloride. Biochemistry. 1976 Apr 4/20/1976; 15(8): 1610-4. PMID: 773424, ISSN: 0006-2960.

Tanphaichitr N, Moore KC, Granner DK, Chalkley R. Relationship between chromosome condensation and metaphase lysine-rich histone phosphorylation. J. Cell Biol. 1976 Apr; 69(1): 43-50. PMID: 1254649, PMCID: PMC2110976, ISSN: 0021-9525.

Chalkley RA, Bloxham DP. Improved alkylation of acetoacetyl-coenzyme A thiolase by extension of chain length in chloromethyl ketone fatty acids. Biochem. Soc. Trans. 1976; 4(2): 279-81. PMID: 12041, ISSN: 0300-5127.

Jackson V, Chalkley R. The effect of urea on staphylococcal nuclease digestion of chromatin. Biochem. Biophys. Res. Commun. 1975 Dec 12/15/1975; 67(4): 1391-400. PMID: 1201093, PII: 0006-291X(75)90181-3, ISSN: 0006-291X.

Jackson V, Granner DK, Chalkley R. Deposition of histones onto replicating chromosomes. Proc. Natl. Acad. Sci. U.S.A. 1975 Nov; 72(11): 4440-4. PMID: 172909, PMCID: PMC388737, ISSN: 0027-8424.

Hardison RC, Eichner ME, Chalkley R. An approach to histone nearest neighbours in extended chromatin. Nucleic Acids Res. 1975 Oct; 2(10): 1751-70. PMID: 1187345, PMCID: PMC343543, ISSN: 0305-1048.

Jackson V, Shires A, Chalkley R, Granner DK. Studies on highly metabolically active acetylation and phosphorylation of histones. J. Biol. Chem. 1975 Jul 7/10/1975; 250(13): 4856-63. PMID: 168194, ISSN: 0021-9258.

Shires A, Carpenter MP, Chalkley R. New histones found in mature mammalian testes. Proc. Natl. Acad. Sci. U.S.A. 1975 Jul; 72(7): 2714-8. PMID: 170615, PMCID: PMC432841, ISSN: 0027-8424.

Chalkley R. Histone propinquity using imidoesters. Biochem. Biophys. Res. Commun. 1975 May 5/19/1975; 64(2): 587-94. PMID: 1147944, PII: 0006-291X(75)90362-9, ISSN: 0006-291X.

Chalkley R, Hunter C. Histone-histone propinquity by aldehyde fixation of chromatin. Proc. Natl. Acad. Sci. U.S.A. 1975 Apr; 72(4): 1304-8. PMID: 805426, PMCID: PMC432521, ISSN: 0027-8424.

Barnett JE, Holman GD, Chalkley RA, Munday KA. Evidence for two asymmetric conformational states in the human erythrocyte sugar-transport system. Biochem. J. 1975 Mar; 145(3): 417-29. PMID: 1156368, PMCID: PMC1165241, ISSN: 0264-6021.

Sherod D, Johnson G, Balhorn R, Jackson V, Chalkley R, Granner D. The phosphorylation region of lysine-rich histone in dividing cells. Biochim. Biophys. Acta. 1975 Feb 2/13/1975; 381(2): 337-47. PMID: 1111593, PII: 0304-4165(75)90239-1, ISSN: 0006-3002.

Balhorn R, Chalkley R. Methods for the assessment of selective histone phosphorylation. Meth. Enzymol. 1975; 40: 138-44. PMID: 1123992, PII: S0076-6879(75)40012-X, ISSN: 0076-6879.

Oliver D, Chalkley R. Asymmetric distribution of histone on DNA: a model for nucleohistone primary structure. Biochemistry. 1974 Dec 12/3/1974; 13(25): 5093-8. PMID: 4474000, ISSN: 0006-2960.

Tanphaichitr N, Balhorn R, Granner D, Chalkley R. Further studies of histone phosphorylation in the presence of inhibitors of DNA synthesis. Biochemistry. 1974 Oct 10/8/1974; 13(21): 4249-54. PMID: 4370034, ISSN: 0006-2960.

Jackson V, Chalkley R. Separation of newly synthesized nucleohistone by equilibrium centrifugation in cesium chloride. Biochemistry. 1974 Sep 9/10/1974; 13(19): 3952-6. PMID: 4370363, ISSN: 0006-2960.

Sherod D, Johnson G, Chalkley R. Studies on the hetrogeneity of lysine-rich histones in dividing cells. J. Biol. Chem. 1974 Jun 6/25/1974; 249(12): 3923-31. PMID: 4833750, ISSN: 0021-9258.

Jackson V, Chalkley R. The binding of estradiol-17 beta to the bovine endometrial nuclear membrane. J. Biol. Chem. 1974 Mar 3/10/1974; 249(5): 1615-26. PMID: 4856342, ISSN: 0021-9258.

Jackson V, Chalkley R. The cytoplasmic estradiol receptors of bovine uterus. Their occurrence, interconversion, and binding properties. J. Biol. Chem. 1974 Mar 3/10/1974; 249(5): 1627-36. PMID: 4361742, ISSN: 0021-9258.

Sommer KR, Chalkley R. A new method for fractionating histones for physical and chemical studies. Biochemistry. 1974 Feb 2/26/1974; 13(5): 1022-7. PMID: 4813364, ISSN: 0006-2960.

Oliver D, Granner D, Chalkley R. Identification of a distinction between cytoplasmic histone synthesis and subsequent histone deposition within the nucleus. Biochemistry. 1974 Feb 2/12/1974; 13(4): 746-9. PMID: 4359465, ISSN: 0006-2960.

Balhorn R, Tanphaichitr N, Chalkley R, Granner DK. The effect of inhibition of deoxyribonucleic acid synthesis on histone phosphorylation. Biochemistry. 1973 Dec 12/4/1973; 12(25): 5146-50. PMID: 4792299, ISSN: 0006-2960.

Hooper JA, Smith EL, Sommer KR, Chalkley R. Histone 3. IV. Amino acid sequence of histone 3 of the testes of the carp, Letiobus bubalus. J. Biol. Chem. 1973 May 5/10/1973; 248(9): 3275-9. PMID: 4700460, ISSN: 0021-9258.

Bartley J, Chalkley R. An approach to the structure of native nucleohistone. Biochemistry. 1973 Jan 1/30/1973; 12(3): 468-74. PMID: 4734233, ISSN: 0006-2960.

Oliver D, Balhorn R, Granner D, Chalkley R. Molecular nature of F 1 histone phosphorylation in cultured hepatoma cells. Biochemistry. 1972 Oct 10/10/1972; 11(21): 3921-5. PMID: 4342898, ISSN: 0006-2960.

Balhorn R, Oliver D, Hohmann P, Chalkley R, Granner D. Turnover of deoxyribonucleic acid, histones, and lysine-rich histone phosphate in hepatoma tissue culture cells. Biochemistry. 1972 Oct 10/10/1972; 11(21): 3915-21. PMID: 4342897, ISSN: 0006-2960.

Balhorn R, Balhorn M, Chalkley R. Lysine-rach histone phosphorylation and hyperplasia in the developing rat. Dev. Biol. 1972 Oct; 29(2): 199-203. PMID: 5075788, ISSN: 0012-1606.

Chattoraj DK, Bull HB, Chalkley R. Binding of histone to DNA. Arch. Biochem. Biophys. 1972 Oct; 152(2): 778-85. PMID: 4673819, PII: 0003-9861(72)90273-1, ISSN: 0003-9861.

Oliver D, Sommer KR, Panyim S, Spiker S, Chalkley R. A modified procedure for fractionating histones. Biochem. J. 1972 Sep; 129(2): 349-53. PMID: 4629976, PMCID: PMC1174084, ISSN: 0264-6021.

Balhorn R, Balhorn M, Morris HP, Chalkley R. Comparative high-resolution electrophoresis of tumor histones: variation in phosphorylation as a function of cell replication rate. Cancer Res. 1972 Aug; 32(8): 1775-84. PMID: 5044136, ISSN: 0008-5472.

Oliver DR, Chalkley R. An electrophoretic analysis of Drosophila histones. II. Comparison of larval and adult histone patterns in two species of Drosophila. Exp. Cell Res. 1972 Aug; 73(2): 303-10. PMID: 4626576, ISSN: 0014-4827.

Oliver D, Chalkley R. An electrophoretic analysis of Drosophila histones. I. Isolation and identification. Exp. Cell Res. 1972 Aug; 73(2): 295-302. PMID: 4626575, ISSN: 0014-4827.

Bartley JA, Chalkley R. The binding of deoxyribonucleic acid and histone in native nucleohistone. J. Biol. Chem. 1972 Jun 6/10/1972; 247(11): 3647-55. PMID: 5030635, ISSN: 0021-9258.

Easton D, Chalkley R. High-resolution electrophoretic analysis of the histones from embryos and sperm of Arbacia punctulata. Exp. Cell Res. 1972 Jun; 72(2): 502-8. PMID: 5037931, ISSN: 0014-4827.

Balhorn R, Chalkley R, Granner D. Lysine-rich histone phosphorylation. A positive correlation with cell replication. Biochemistry. 1972 Mar 3/14/1972; 11(6): 1094-8. PMID: 4335288, ISSN: 0006-2960.

Balhorn R, Bordwell J, Sellers L, Granner D, Chalkley R. Histone phosphorylation and DNA synthesis are linked in synchronous cultures of HTC cells. Biochem. Biophys. Res. Commun. 1972 Feb 2/16/1972; 46(3): 1326-33. PMID: 4334977, PII: S0006-291X(72)80120-7, ISSN: 0006-291X.

Panyim S, Chalkley R. The molecular weights of vertebrate histones exploiting a modified sodium dodecyl sulfate electrophoretic method. J. Biol. Chem. 1971 Dec 12/25/1971; 246(24): 7557-60. PMID: 5135315, ISSN: 0021-9258.

Granner DK, Belhorn R, Chalkley R. Histone phosphorylation and cell division. J. Lab. Clin. Med. 1971 Dec; 78(6): 992-3. PMID: 5131874, ISSN: 0022-2143.

Oliver D, Chalkley R. An improved photographic system for polyacrylamide gels. Anal. Biochem. 1971 Dec; 44(2): 540-2. PMID: 5130943, ISSN: 0003-2697.

Panyim S, Sommer KR, Chalkley R. Oxidation of the cysteine-containing histone F3. Detection of an evolutionary mutation in a conservative histone. Biochemistry. 1971 Oct 10/12/1971; 10(21): 3911-7. PMID: 5160417, ISSN: 0006-2960.

Balhorn R, Rieke WO, Chalkley R. Rapid electrophoretic analysis for histone phosphorylation. A reinvestigation of phosphorylation of lysine-rich histone during rat liver regeneration. Biochemistry. 1971 Oct 10/12/1971; 10(21): 3952-9. PMID: 4946191, ISSN: 0006-2960.

Panyim S, Bilek D, Chalkley R. An electrophoretic comparison of vertebrate histones. J. Biol. Chem. 1971 Jul 7/10/1971; 246(13): 4206-15. PMID: 5090040, ISSN: 0021-9258.

Stegink LD, Meyer PD, Chalkley R. Acrylamide gel electrophoresis of hemoglobin polypeptide chains. Anal. Biochem. 1971 Jun; 41(2): 351-9. PMID: 5092160, ISSN: 0003-2697.

Sherod D, Johnson G, Chalkley R. Phosphorylation of mouse ascites tumor cell lysine-rich histone. Biochemistry. 1970 Nov 11/10/1970; 9(23): 4611-5. PMID: 5474151, ISSN: 0006-2960.

Bartley J, Chalkley R. Further studies of a thymus nucleohistone-associated protease. J. Biol. Chem. 1970 Sep 9/10/1970; 245(17): 4286-92. PMID: 5498413, ISSN: 0021-9258.

Panyim S, Chalkley R, Spiker S, Oliver D. Constant electrophoretic mobility of the cysteine-containing histone in plants and animals. Biochim. Biophys. Acta. 1970 Jul 7/27/1970; 214(1): 216-21. PMID: 5530755, ISSN: 0006-3002.

Panyim S, Chalkley R. A new histone found only in mammalian tissues with little cell division. Biochem. Biophys. Res. Commun. 1969 Dec 12/4/1969; 37(6): 1042-9. PMID: 5391215, PII: 0006-291X(69)90237-X, ISSN: 0006-291X.

Panyim S, Chalkley R. The heterogeneity of histones. I. A quantitative analysis of calf histones in very long polyacrylamide gels. Biochemistry. 1969 Oct; 8(10): 3972-9. PMID: 5346380, ISSN: 0006-2960.

Panyim S, Chalkley R. High resolution acrylamide gel electrophoresis of histones. Arch. Biochem. Biophys. 1969 Mar; 130(1): 337-46. PMID: 5778650, ISSN: 0003-9861.

Jensen RH, Chalkley R. The physical state of nucleohistone under physiological ionic strength. The effect of interaction with free nucleic acids. Biochemistry. 1968 Dec; 7(12): 4388-95. PMID: 5700663, ISSN: 0006-2960.

Chalkley R, Jensen RH. A study of the structure of isolated chromatin. Biochemistry. 1968 Dec; 7(12): 4380-8. PMID: 5700662, ISSN: 0006-2960.

Jackson V, Earnhardt J, Chalkley R. A DNA-lipid protein containing material isolated from calf thymus nuclear chromatin. Biochem. Biophys. Res. Commun. 1968 Oct 10/24/1968; 33(2): 253-9. PMID: 5722220, PII: 0006-291X(68)90777-8, ISSN: 0006-291X.

Panyim S, Jensen RH, Chalkley R. Proteolytic contamination of calf thymus nucleohistone and its inhibition. Biochim. Biophys. Acta. 1968 Jun 6/26/1968; 160(2): 252-5. PMID: 5658127, ISSN: 0006-3002.

Bartley JA, Chalkley R. The viscosity of nucleohistone in urea. Biochim. Biophys. Acta. 1968 Jun 6/26/1968; 160(2): 224-8. PMID: 5658124, ISSN: 0006-3002.

Chalkley R. Histones in relation to control in living systems. NASA CR-673. NASA Contract Rep NASA CR. 1967 Feb; 131-47. PMID: 5297964, ISSN: 0565-7059.