Wael El-Rifai, M.D., Ph.D.


Faculty Appointments
Professor of Surgery Professor of Cancer BiologyH. William Scott, Jr., Chair in Surgery
Ph.D., Cancer Genetics, University of Helsinki, Helsinki, FinlandM.D., Medicine, Ain Shams University, Cairo, EgyptM.Sc., Human Genetics, Ain Shams University, Cairo, Egypt
Office Address
2215 Garland Avenue
1255 Light Hall
Nashville, TN 37232-2733
Research Description
Wael El-Rifai, MD., Ph.D.
H. William Scott Jr. Chair in Surgery
Professor, Surgery & Cancer Biology
Director, Surgical Oncology Research

Dr. El-Rifai has a long-standing interest in understanding the modulation of molecular pathways through genetic and epigenetic events. His research is focused on upper gastrointestinal carcinomas (UGCs) with two main goals:

1. Translational OncoGenomics in upper gastrointestinal carcinomas
The El-Rifai laboratory has extensive expertise in the dissection of genetic and epigenetic alterations in upper gastrointestinal carcinomas (UGC). This group of tumors includes distal gastric tumors and proximal adenocarcinomas of the gastroesophageal junctional (GEJ) and lower esophagus. Proximal adenocarcinomas are the fastest rising tumors in the United States as well as in the rest of the western world. In my laboratory, we apply comprehensive, molecular-analysis-approaches with the integration of data in order to understand the molecular anatomy of these tumors. Several high-throughput technologies are utilized in his research. These include comparative genomic hybridization, microarrays, serial analysis of gene expression, and proteomic approaches. The El-Rifai laboratory identified a number of genomic hot spots and has systematically characterized the 1q, 17q, and 20q amplicons by combining the genomic data with gene expression data. At 17q21 he identified and characterized a 168 kb critical amplicon region that contains several known oncogenes side-by-side such as ERBB2, GRB7, and TOP2A. We have collected more than 400 tumors on tissue microarrays. These TMAs are continuously analyzed for identification of the clinical and histopathological significance of molecular alterations. Dr. El-Rifai’s lab was the first group of investigators to reveal Darpp-32 and its isoforms as novel cancer genes.
Our research extends to encompass animal models as tools of identification of tumor development and progression. In this direction, we have obtained TFF1 knockout mice that develop premalignant gastric dysplasia. Dr. El-Rifai laboratory has characterized this model and used it to identify alterations in molecular pathways that occur in a premalignant state. Using this approach, his research group discovered a number of previously unknown genes in gastric tumorigenesis and was able to validate the findings in human premalignant conditions. These genes include GPx3, Claudin-7, and Caveolin.
Dr. El-Rifai has demonstrated DNA promoter hypermethylation as a mechanism of down-regulation and loss of expression of several novel genes such as MT3, MGMT, DAPK, and GPX3. He has further shown that some of these changes are early events initiated in premalignant states such as in Barrett’s dysplasia. Recently, we have added to our laboratory the cutting-edge, pyrosequencing technology (Biotage) for a high through-put, robust, quantitative analysis of promoter CpG methylation sites.

2. Cellular effects and signaling transduction in UGCs
In line with his translational research component, Dr. El-Rifai laboratory is primarily interested in identifying anti-apoptotic/pro-survival targets that contribute to drug resistance. At this time, the work is focused in understanding the p53-dependent and independent mechanisms that contribute to this phenotype. Our work has demonstrated that Darpp-32 is a potent antiapoptotic gene that provides a phenotype that is drug resistant to cancer cells against several chemotherapeutic agents. This drug resistant phenotype was through a p53-independent mechanism that included up-regulation and activation of mitochondrial Bcl2 prosurvival proteins. In my laboratory, we use normal and transformed cells, specific antibodies and purified protein kinases to show phosphorylation controls activity. We continue to establish the oncogenic properties and downstream targets of Darpp-32 and/or t-Darpp in order to understand the signaling pathways that are regulated by Darpp-32 in cancer. In addition to studies on Dar
Research Keywords
Cancer Genetics, Molecular Biology, upper gastrointestinal cancer, Barrett's adenocarcinoma, gastric cancer
Soutto M, Chen Z, Katsha AM, Romero-Gallo J, Krishna US, Piazuelo MB, Washington MK, Peek RM, Belkhiri A, El-Rifai WM. Trefoil factor 1 expression suppresses Helicobacter pylori-induced inflammation in gastric carcinogenesis. Cancer [print-electronic]. 2015 Dec 12/15/2015; 121(24): 4348-58. PMID: 26372254, PMCID: PMC4670586, DOI: 10.1002/cncr.29644, ISSN: 1097-0142.

Taylor C, Loomans HA, Le Bras GF, Koumangoye RB, Romero-Morales AI, Quast LL, Zaika AI, El-Rifai W, Andl T, Andl CD. Activin a signaling regulates cell invasion and proliferation in esophageal adenocarcinoma. Oncotarget. 2015 Oct 10/27/2015; 6(33): 34228-44. PMID: 26447543, PII: 5349, DOI: 10.18632/oncotarget.5349, ISSN: 1949-2553.

Schneider BG, Mera R, Piazuelo MB, Bravo JC, Zabaleta J, Delgado AG, Bravo LE, Wilson KT, El-Rifai W, Peek RM, Correa P. DNA Methylation Predicts Progression of Human Gastric Lesions. Cancer Epidemiol. Biomarkers Prev [print-electronic]. 2015 Oct; 24(10): 1607-13. PMID: 26269563, PMCID: PMC4592454, PII: 1055-9965.EPI-15-0388, DOI: 10.1158/1055-9965.EPI-15-0388, ISSN: 1538-7755.

Soutto M, Romero-Gallo J, Krishna U, Piazuelo MB, Washington MK, Belkhiri A, Peek RM, El-Rifai W. Loss of TFF1 promotes Helicobacter pylori-induced ß-catenin activation and gastric tumorigenesis. Oncotarget. 2015 Jul 7/20/2015; 6(20): 17911-22. PMID: 25980439, PMCID: PMC4627225, PII: 3772, DOI: 10.18632/oncotarget.3772, ISSN: 1949-2553.

Soutto M, Peng D, Katsha A, Chen Z, Piazuelo MB, Washington MK, Belkhiri A, Correa P, El-Rifai W. Activation of ß-catenin signalling by TFF1 loss promotes cell proliferation and gastric tumorigenesis. Gut [print-electronic]. 2015 Jul; 64(7): 1028-39. PMID: 25107557, PMCID: PMC4320984, PII: gutjnl-2014-307191, DOI: 10.1136/gutjnl-2014-307191, ISSN: 1468-3288.

Wei J, Noto JM, Zaika E, Romero-Gallo J, Piazuelo MB, Schneider B, El-Rifai W, Correa P, Peek RM, Zaika AI. Bacterial CagA protein induces degradation of p53 protein in a p14ARF-dependent manner. Gut [print-electronic]. 2015 Jul; 64(7): 1040-8. PMID: 25080447, PMCID: PMC4312278, PII: gutjnl-2014-307295, DOI: 10.1136/gutjnl-2014-307295, ISSN: 1468-3288.

Zhu S, Chen Z, Katsha A, Hong J, Belkhiri A, El-Rifai W. Regulation of CD44E by DARPP-32-dependent activation of SRp20 splicing factor in gastric tumorigenesis. Oncogene [print-electronic]. 2015 Jun 6/29/2015; PMID: 26119931, PMCID: PMC4486340, PII: onc2015250, DOI: 10.1038/onc.2015.250, ISSN: 1476-5594.

Chen Z, Zhu S, Hong J, Soutto M, Peng D, Belkhiri A, Xu Z, El-Rifai W. Gastric tumour-derived ANGPT2 regulation by DARPP-32 promotes angiogenesis. Gut [print-electronic]. 2015 Mar 3/16/2015; PMID: 25779598, PMCID: PMC4573388, PII: gutjnl-2014-308416, DOI: 10.1136/gutjnl-2014-308416, ISSN: 1468-3288.

Bhardwaj V, Noto JM, Wei J, Andl C, El-Rifai W, Peek RM, Zaika AI. Helicobacter pylori bacteria alter the p53 stress response via ERK-HDM2 pathway. Oncotarget. 2015 Jan 1/30/2015; 6(3): 1531-43. PMID: 25605238, PMCID: PMC4359312, PII: 2828, DOI: 10.18632/oncotarget.2828, ISSN: 1949-2553.

Belkhiri A, El-Rifai W. Advances in targeted therapies and new promising targets in esophageal cancer. Oncotarget. 2015 Jan 1/30/2015; 6(3): 1348-58. PMID: 25593196, PMCID: PMC4359299, PII: 2752, DOI: 10.18632/oncotarget.2752, ISSN: 1949-2553.

Katsha A, Belkhiri A, Goff L, El-Rifai W. Aurora kinase A in gastrointestinal cancers: time to target. Mol. Cancer. 2015; 14: 106. PMID: 25987188, PMCID: PMC4436812, PII: 10.1186/s12943-015-0375-4, DOI: 10.1186/s12943-015-0375-4, ISSN: 1476-4598.

Katsha A, Arras J, Soutto M, Belkhiri A, El-Rifai W. AURKA regulates JAK2-STAT3 activity in human gastric and esophageal cancers. Mol Oncol [print-electronic]. 2014 Dec; 8(8): 1419-28. PMID: 24953013, PMCID: PMC4254172, PII: S1574-7891(14)00119-7, DOI: 10.1016/j.molonc.2014.05.012, ISSN: 1878-0261.

Soutto M, Chen Z, Saleh MA, Katsha A, Zhu S, Zaika A, Belkhiri A, El-Rifai W. TFF1 activates p53 through down-regulation of miR-504 in gastric cancer. Oncotarget. 2014 Jul 7/30/2014; 5(14): 5663-73. PMID: 25015107, PMCID: PMC4170596, PII: 2156, DOI: 10.18632/oncotarget.2156, ISSN: 1949-2553.

Peng DF, Hu TL, Soutto M, Belkhiri A, El-Rifai W. Loss of glutathione peroxidase 7 promotes TNF-a-induced NF-¿B activation in Barrett's carcinogenesis. Carcinogenesis [print-electronic]. 2014 Jul; 35(7): 1620-8. PMID: 24692067, PMCID: PMC4076814, PII: bgu083, DOI: 10.1093/carcin/bgu083, ISSN: 1460-2180.

Belkhiri A, El-Rifai W. 5-Methylcytosine hydroxylation-mediated LINE-1 hypomethylation: a novel mechanism of proto-oncogenes activation in colorectal cancer?. Gut [print-electronic]. 2014 Apr; 63(4): 538-9. PMID: 23812322, PMCID: PMC4035300, PII: gutjnl-2013-305176, DOI: 10.1136/gutjnl-2013-305176, ISSN: 1468-3288.

Peng D, Hu T, Soutto M, Belkhiri A, Zaika A, El-Rifai W. Glutathione peroxidase 7 has potential tumour suppressor functions that are silenced by location-specific methylation in oesophageal adenocarcinoma. Gut [print-electronic]. 2014 Apr; 63(4): 540-51. PMID: 23580780, PMCID: PMC3825783, PII: gutjnl-2013-304612, DOI: 10.1136/gutjnl-2013-304612, ISSN: 1468-3288.

Sehdev V, Katsha A, Arras J, Peng D, Soutto M, Ecsedy J, Zaika A, Belkhiri A, El-Rifai W. HDM2 regulation by AURKA promotes cell survival in gastric cancer. Clin. Cancer Res [print-electronic]. 2014 Jan 1/1/2014; 20(1): 76-86. PMID: 24240108, PMCID: PMC3947328, PII: 1078-0432.CCR-13-1187, DOI: 10.1158/1078-0432.CCR-13-1187, ISSN: 1078-0432.

Peng DF, Hu TL, Soutto M, Belkhiri A, El-Rifai W. Glutathione Peroxidase 7 Suppresses Bile Salt-Induced Expression of Pro-Inflammatory Cytokines in Barrett's Carcinogenesis. J Cancer. 2014; 5(7): 510-7. PMID: 24963355, PMCID: PMC4067510, PII: jcav05p0510, DOI: 10.7150/jca.9215, ISSN: 1837-9664.

Mohamed MM, Sabet S, Peng DF, Nouh MA, El-Shinawi M, El-Rifai W. Promoter hypermethylation and suppression of glutathione peroxidase 3 are associated with inflammatory breast carcinogenesis. Oxid Med Cell Longev [print-electronic]. 2014; 2014: 787195. PMID: 24790704, PMCID: PMC3980917, DOI: 10.1155/2014/787195, ISSN: 1942-0994.

Katsha A, Soutto M, Sehdev V, Peng D, Washington MK, Piazuelo MB, Tantawy MN, Manning HC, Lu P, Shyr Y, Ecsedy J, Belkhiri A, El-Rifai W. Aurora kinase A promotes inflammation and tumorigenesis in mice and human gastric neoplasia. Gastroenterology [print-electronic]. 2013 Dec; 145(6): 1312-22.e1. PMID: 23993973, PMCID: PMC3840093, PII: S0016-5085(13)01270-5, DOI: 10.1053/j.gastro.2013.08.050, ISSN: 1528-0012.

Erdogan B, Bosompem A, Peng D, Han L, Smith E, Kennedy ME, Alford CE, Wu H, Zhao Z, Mosse CA, El-Rifai W, Kim AS. Methylation of promoters of microRNAs and their host genes in myelodysplastic syndromes. Leuk. Lymphoma [print-electronic]. 2013 Dec; 54(12): 2720-7. PMID: 23547841, PMCID: PMC4120331, DOI: 10.3109/10428194.2013.790542, ISSN: 1029-2403.

Schneider BG, Piazuelo MB, Sicinschi LA, Mera R, Peng DF, Roa JC, Romero-Gallo J, Delgado AG, de Sablet T, Bravo LE, Wilson KT, El-Rifai W, Peek RM, Correa P. Virulence of infecting Helicobacter pylori strains and intensity of mononuclear cell infiltration are associated with levels of DNA hypermethylation in gastric mucosae. Epigenetics [print-electronic]. 2013 Nov; 8(11): 1153-61. PMID: 24128875, PMCID: PMC3927747, PII: 26072, DOI: 10.4161/epi.26072, ISSN: 1559-2308.

Lim J, Duong T, Lee G, Seong BL, El-Rifai W, Ruley HE, Jo D. The effect of intracellular protein delivery on the anti-tumor activity of recombinant human endostatin. Biomaterials [print-electronic]. 2013 Aug; 34(26): 6261-71. PMID: 23714245, PII: S0142-9612(13)00565-6, DOI: 10.1016/j.biomaterials.2013.05.011, ISSN: 1878-5905.

Chen Z, Saad R, Jia P, Peng D, Zhu S, Washington MK, Zhao Z, Xu Z, El-Rifai W. Gastric adenocarcinoma has a unique microRNA signature not present in esophageal adenocarcinoma. Cancer [print-electronic]. 2013 Jun 6/1/2013; 119(11): 1985-93. PMID: 23456798, PMCID: PMC3731210, DOI: 10.1002/cncr.28002, ISSN: 1097-0142.

Cleveland SM, Smith S, Tripathi R, Mathias EM, Goodings C, Elliott N, Peng D, El-Rifai W, Yi D, Chen X, Li L, Mullighan C, Downing JR, Love P, Davé UP. Lmo2 induces hematopoietic stem cell-like features in T-cell progenitor cells prior to leukemia. Stem Cells. 2013 May; 31(5): 882-94. PMID: 23378057, PMCID: PMC3652616, DOI: 10.1002/stem.1345, ISSN: 1549-4918.

O'Neal RL, Nam KT, LaFleur BJ, Barlow B, Nozaki K, Lee HJ, Kim WH, Yang HK, Shi C, Maitra A, Montgomery E, Washington MK, El Rifai W, Drapkin RI, Goldenring JR. Human epididymis protein 4 is up-regulated in gastric and pancreatic adenocarcinomas. Hum. Pathol [print-electronic]. 2013 May; 44(5): 734-42. PMID: 23084584, PMCID: PMC3556378, PII: S0046-8177(12)00275-4, DOI: 10.1016/j.humpath.2012.07.017, ISSN: 1532-8392.

Sehdev V, Katsha A, Ecsedy J, Zaika A, Belkhiri A, El-Rifai W. The combination of alisertib, an investigational Aurora kinase A inhibitor, and docetaxel promotes cell death and reduces tumor growth in preclinical cell models of upper gastrointestinal adenocarcinomas. Cancer [print-electronic]. 2013 Feb 2/15/2013; 119(4): 904-14. PMID: 22972611, PMCID: PMC3524359, DOI: 10.1002/cncr.27801, ISSN: 1097-0142.

Lim J, Duong T, Do N, Do P, Kim J, Kim H, El-Rifai W, Ruley HE, Jo D. Antitumor activity of cell-permeable RUNX3 protein in gastric cancer cells. Clin. Cancer Res [print-electronic]. 2013 Feb 2/1/2013; 19(3): 680-90. PMID: 23230322, PMCID: PMC4039637, PII: 1078-0432.CCR-12-2692, DOI: 10.1158/1078-0432.CCR-12-2692, ISSN: 1078-0432.

Saad R, Chen Z, Zhu S, Jia P, Zhao Z, Washington MK, Belkhiri A, El-Rifai W. Deciphering the unique microRNA signature in human esophageal adenocarcinoma. PLoS ONE [electronic-print]. 2013; 8(5): e64463. PMID: 23724052, PMCID: PMC3665888, PII: PONE-D-12-35460, DOI: 10.1371/journal.pone.0064463, ISSN: 1932-6203.

Zaika E, Bhardwaj V, Wei J, Washington MK, Souza R, El-Rifai W, Zaika A. Proinflammatory cytokines and bile acids upregulate ¿Np73 protein, an inhibitor of p53 and p73 tumor suppressors. PLoS ONE [electronic-print]. 2013; 8(5): e64306. PMID: 23717592, PMCID: PMC3661465, PII: PONE-D-13-03381, DOI: 10.1371/journal.pone.0064306, ISSN: 1932-6203.

Zhu S, Hong J, Tripathi MK, Sehdev V, Belkhiri A, El-Rifai W. Regulation of CXCR4-mediated invasion by DARPP-32 in gastric cancer cells. Mol. Cancer Res [print-electronic]. 2013 Jan; 11(1): 86-94. PMID: 23160836, PMCID: PMC3552044, PII: 1541-7786.MCR-12-0243-T, DOI: 10.1158/1541-7786.MCR-12-0243-T, ISSN: 1557-3125.

Wei J, Noto J, Zaika E, Romero-Gallo J, Correa P, El-Rifai W, Peek RM, Zaika A. Pathogenic bacterium Helicobacter pylori alters the expression profile of p53 protein isoforms and p53 response to cellular stresses. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2012 Sep 9/18/2012; 109(38): E2543-50. PMID: 22927405, PMCID: PMC3458371, PII: 1205664109, DOI: 10.1073/pnas.1205664109, ISSN: 1091-6490.

Hong J, Katsha A, Lu P, Shyr Y, Belkhiri A, El-Rifai W. Regulation of ERBB2 receptor by t-DARPP mediates trastuzumab resistance in human esophageal adenocarcinoma. Cancer Res [print-electronic]. 2012 Sep 9/1/2012; 72(17): 4504-14. PMID: 22745369, PMCID: PMC3432752, PII: 0008-5472.CAN-12-1119, DOI: 10.1158/0008-5472.CAN-12-1119, ISSN: 1538-7445.

Peng D, Belkhiri A, Hu T, Chaturvedi R, Asim M, Wilson KT, Zaika A, El-Rifai W. Glutathione peroxidase 7 protects against oxidative DNA damage in oesophageal cells. Gut [print-electronic]. 2012 Sep; 61(9): 1250-60. PMID: 22157330, PMCID: PMC3419307, PII: gutjnl-2011-301078, DOI: 10.1136/gutjnl-2011-301078, ISSN: 1468-3288.

Rau TT, Rogler A, Frischauf M, Jung A, Konturek PC, Dimmler A, Faller G, Sehnert B, El-Rifai W, Hartmann A, Voll RE, Schneider-Stock R. Methylation-dependent activation of CDX1 through NF-¿B: a link from inflammation to intestinal metaplasia in the human stomach. Am. J. Pathol [print-electronic]. 2012 Aug; 181(2): 487-98. PMID: 22749770, PMCID: PMC3409443, PII: S0002-9440(12)00413-0, DOI: 10.1016/j.ajpath.2012.04.028, ISSN: 1525-2191.

Belkhiri A, Zhu S, Chen Z, Soutto M, El-Rifai W. Resistance to TRAIL is mediated by DARPP-32 in gastric cancer. Clin. Cancer Res [print-electronic]. 2012 Jul 7/15/2012; 18(14): 3889-900. PMID: 22589394, PMCID: PMC3399047, PII: 1078-0432.CCR-11-3182, DOI: 10.1158/1078-0432.CCR-11-3182, ISSN: 1078-0432.

Sehdev V, Peng D, Soutto M, Washington MK, Revetta F, Ecsedy J, Zaika A, Rau TT, Schneider-Stock R, Belkhiri A, El-Rifai W. The aurora kinase A inhibitor MLN8237 enhances cisplatin-induced cell death in esophageal adenocarcinoma cells. Mol. Cancer Ther [print-electronic]. 2012 Mar; 11(3): 763-74. PMID: 22302096, PMCID: PMC3297687, PII: 1535-7163.MCT-11-0623, DOI: 10.1158/1535-7163.MCT-11-0623, ISSN: 1538-8514.

Peng DF, Hu TL, Schneider BG, Chen Z, Xu ZK, El-Rifai W. Silencing of glutathione peroxidase 3 through DNA hypermethylation is associated with lymph node metastasis in gastric carcinomas. PLoS ONE [print-electronic]. 2012; 7(10): e46214. PMID: 23071548, PMCID: PMC3468580, PII: PONE-D-12-10711, DOI: 10.1371/journal.pone.0046214, ISSN: 1932-6203.

Feng J, Pan X, Yu J, Chen Z, Xu H, El-Rifai W, Zhang G, Xu Z. Functional PstI/RsaI polymorphism in CYP2E1 is associated with the development, progression and poor outcome of gastric cancer. PLoS ONE [print-electronic]. 2012; 7(9): e44478. PMID: 22957075, PMCID: PMC3434136, PII: PONE-D-12-14810, DOI: 10.1371/journal.pone.0044478, ISSN: 1932-6203.

Zaika E, Wei J, Yin D, Andl C, Moll U, El-Rifai W, Zaika AI. P73 protein regulates DNA damage repair. FASEB J [print-electronic]. 2011 Dec; 25(12): 4406-14. PMID: 21891782, PMCID: PMC3236618, PII: fj.11-192815, DOI: 10.1096/fj.11-192815, ISSN: 1530-6860.

Zhu S, Belkhiri A, El-Rifai W. DARPP-32 increases interactions between epidermal growth factor receptor and ERBB3 to promote tumor resistance to gefitinib. Gastroenterology [print-electronic]. 2011 Nov; 141(5): 1738-48.e1. PMID: 21741919, PMCID: PMC3202055, PII: S0016-5085(11)00920-6, DOI: 10.1053/j.gastro.2011.06.070, ISSN: 1528-0012.

Williams CS, Zhang B, Smith JJ, Jayagopal A, Barrett CW, Pino C, Russ P, Presley SH, Peng D, Rosenblatt DO, Haselton FR, Yang JL, Washington MK, Chen X, Eschrich S, Yeatman TJ, El-Rifai W, Beauchamp RD, Chang MS. BVES regulates EMT in human corneal and colon cancer cells and is silenced via promoter methylation in human colorectal carcinoma. J. Clin. Invest [print-electronic]. 2011 Oct; 121(10): 4056-69. PMID: 21911938, PMCID: PMC3195453, PII: 44228, DOI: 10.1172/JCI44228, ISSN: 1558-8238.

Ueda Y, Hajri T, Peng D, Marks-Shulman PA, Tamboli RA, Shukrallah B, Saliba J, Jabbour K, El-Rifai W, Abumrad NA, Abumrad NN. Reduction of 8-iso-prostaglandin F2a in the first week after Roux-en-Y gastric bypass surgery. Obesity (Silver Spring) [print-electronic]. 2011 Aug; 19(8): 1663-8. PMID: 21475145, PMCID: PMC3176330, PII: oby201158, DOI: 10.1038/oby.2011.58, ISSN: 1930-739X.

Soutto M, Belkhiri A, Piazuelo MB, Schneider BG, Peng D, Jiang A, Washington MK, Kokoye Y, Crowe SE, Zaika A, Correa P, Peek RM, El-Rifai W. Loss of TFF1 is associated with activation of NF-¿B-mediated inflammation and gastric neoplasia in mice and humans. J. Clin. Invest [print-electronic]. 2011 May; 121(5): 1753-67. PMID: 21490402, PMCID: PMC3083788, PII: 43922, DOI: 10.1172/JCI43922, ISSN: 1558-8238.

Peng D, Hu TL, Jiang A, Washington MK, Moskaluk CA, Schneider-Stock R, El-Rifai W. Location-specific epigenetic regulation of the metallothionein 3 gene in esophageal adenocarcinomas. PLoS ONE [print-electronic]. 2011; 6(7): e22009. PMID: 21818286, PMCID: PMC3139601, PII: PONE-D-11-06954, DOI: 10.1371/journal.pone.0022009, ISSN: 1932-6203.

Vangamudi B, Zhu S, Soutto M, Belkhiri A, El-Rifai W. Regulation of ß-catenin by t-DARPP in upper gastrointestinal cancer cells. Mol. Cancer. 2011; 10: 32. PMID: 21447180, PMCID: PMC3075216, PII: 1476-4598-10-32, DOI: 10.1186/1476-4598-10-32, ISSN: 1476-4598.

Mukherjee K, Chakravarthy AB, Goff LW, El-Rifai W. Esophageal adenocarcinoma: treatment modalities in the era of targeted therapy. Dig. Dis. Sci [print-electronic]. 2010 Dec; 55(12): 3304-14. PMID: 20300841, PMCID: PMC2890301, DOI: 10.1007/s10620-010-1187-4, ISSN: 1573-2568.

Schneider BG, Peng DF, Camargo MC, Piazuelo MB, Sicinschi LA, Mera R, Romero-Gallo J, Delgado AG, Bravo LE, Wilson KT, Peek RM, Correa P, El-Rifai W. Promoter DNA hypermethylation in gastric biopsies from subjects at high and low risk for gastric cancer. Int. J. Cancer. 2010 Dec 12/1/2010; 127(11): 2588-97. PMID: 20178103, PMCID: PMC2916942, DOI: 10.1002/ijc.25274, ISSN: 1097-0215.

Vilgelm AE, Hong SM, Washington MK, Wei J, Chen H, El-Rifai W, Zaika A. Characterization of ¿Np73 expression and regulation in gastric and esophageal tumors. Oncogene [print-electronic]. 2010 Oct 10/28/2010; 29(43): 5861-8. PMID: 20676143, PMCID: PMC4152004, PII: onc2010319, DOI: 10.1038/onc.2010.319, ISSN: 1476-5594.

Wei J, Nagy TA, Vilgelm A, Zaika E, Ogden SR, Romero-Gallo J, Piazuelo MB, Correa P, Washington MK, El-Rifai W, Peek RM, Zaika A. Regulation of p53 tumor suppressor by Helicobacter pylori in gastric epithelial cells. Gastroenterology [print-electronic]. 2010 Oct; 139(4): 1333-43. PMID: 20547161, PMCID: PMC2949494, PII: S0016-5085(10)00877-2, DOI: 10.1053/j.gastro.2010.06.018, ISSN: 1528-0012.

Soutto M, Peng D, Razvi M, Ruemmele P, Hartmann A, Roessner A, Schneider-Stock R, El-Rifai W. Epigenetic and genetic silencing of CHFR in esophageal adenocarcinomas. Cancer. 2010 Sep 9/1/2010; 116(17): 4033-42. PMID: 20564104, PMCID: PMC2930066, DOI: 10.1002/cncr.25151, ISSN: 0008-543X.

Mukherjee K, Peng D, Brifkani Z, Belkhiri A, Pera M, Koyama T, Koehler EA, Revetta FL, Washington MK, El-Rifai W. Dopamine and cAMP regulated phosphoprotein MW 32 kDa is overexpressed in early stages of gastric tumorigenesis. Surgery [print-electronic]. 2010 Aug; 148(2): 354-63. PMID: 20580047, PMCID: PMC2919779, PII: S0039-6060(10)00274-6, DOI: 10.1016/j.surg.2010.05.011, ISSN: 1532-7361.

Dar AA, Goff LW, Majid S, Berlin J, El-Rifai W. Aurora kinase inhibitors--rising stars in cancer therapeutics?. Mol. Cancer Ther [print-electronic]. 2010 Feb; 9(2): 268-78. PMID: 20124450, PMCID: PMC2820587, PII: 1535-7163.MCT-09-0765, DOI: 10.1158/1535-7163.MCT-09-0765, ISSN: 1538-8514.

Vangamudi B, Peng DF, Cai Q, El-Rifai W, Zheng W, Belkhiri A. T-DARPP regulates phosphatidylinositol-3-kinase-dependent cell growth in breast cancer. Mol. Cancer. 2010; 9: 240. PMID: 20836878, PMCID: PMC2945963, PII: 1476-4598-9-240, DOI: 10.1186/1476-4598-9-240, ISSN: 1476-4598.

Dar AA, Belkhiri A, El-Rifai W. The aurora kinase A regulates GSK-3beta in gastric cancer cells. Oncogene [print-electronic]. 2009 Feb 2/12/2009; 28(6): 866-75. PMID: 19060929, PMCID: PMC2642527, PII: onc2008434, DOI: 10.1038/onc.2008.434, ISSN: 1476-5594.

Peng DF, Razvi M, Chen H, Washington K, Roessner A, Schneider-Stock R, El-Rifai W. DNA hypermethylation regulates the expression of members of the Mu-class glutathione S-transferases and glutathione peroxidases in Barrett's adenocarcinoma. Gut [print-electronic]. 2009 Jan; 58(1): 5-15. PMID: 18664505, PMCID: PMC2845391, PII: gut.2007.146290, DOI: 10.1136/gut.2007.146290, ISSN: 1468-3288.

Dar AA, Belkhiri A, Ecsedy J, Zaika A, El-Rifai W. Aurora kinase A inhibition leads to p73-dependent apoptosis in p53-deficient cancer cells. Cancer Res. 2008 Nov 11/1/2008; 68(21): 8998-9004. PMID: 18974145, PMCID: PMC2587495, PII: 68/21/8998, DOI: 10.1158/0008-5472.CAN-08-2658, ISSN: 1538-7445.

Vilgelm A, El-Rifai W, Zaika A. Therapeutic prospects for p73 and p63: rising from the shadow of p53. Drug Resist. Updat [print-electronic]. 2008 Aug; 11(4-5): 152-63. PMID: 18801697, PMCID: PMC2585826, PII: S1368-7646(08)00046-0, DOI: 10.1016/j.drup.2008.08.001, ISSN: 1532-2084.

Belkhiri A, Dar AA, Peng DF, Razvi MH, Rinehart C, Arteaga CL, El-Rifai W. Expression of t-DARPP mediates trastuzumab resistance in breast cancer cells. Clin. Cancer Res [print-electronic]. 2008 Jul 7/15/2008; 14(14): 4564-71. PMID: 18579663, PMCID: PMC2842884, PII: 1078-0432.CCR-08-0121, DOI: 10.1158/1078-0432.CCR-08-0121, ISSN: 1078-0432.

Wei J, O'Brien D, Vilgelm A, Piazuelo MB, Correa P, Washington MK, El-Rifai W, Peek RM, Zaika A. Interaction of Helicobacter pylori with gastric epithelial cells is mediated by the p53 protein family. Gastroenterology [print-electronic]. 2008 May; 134(5): 1412-23. PMID: 18343378, PMCID: PMC2430883, PII: S0016-5085(08)00178-9, DOI: 10.1053/j.gastro.2008.01.072, ISSN: 1528-0012.

Dar AA, Zaika A, Piazuelo MB, Correa P, Koyama T, Belkhiri A, Washington K, Castells A, Pera M, El-Rifai W. Frequent overexpression of Aurora Kinase A in upper gastrointestinal adenocarcinomas correlates with potent antiapoptotic functions. Cancer. 2008 Apr 4/15/2008; 112(8): 1688-98. PMID: 18311783, PMCID: PMC4030394, DOI: 10.1002/cncr.23371, ISSN: 0008-543X.

Vilgelm A, Wei JX, Piazuelo MB, Washington MK, Prassolov V, El-Rifai W, Zaika A. DeltaNp73alpha regulates MDR1 expression by inhibiting p53 function. Oncogene [print-electronic]. 2008 Apr 4/3/2008; 27(15): 2170-6. PMID: 17952118, PMCID: PMC4066670, PII: 1210862, DOI: 10.1038/sj.onc.1210862, ISSN: 1476-5594.

Peng D, Sheta EA, Powell SM, Moskaluk CA, Washington K, Goldknopf IL, El-Rifai W. Alterations in Barrett's-related adenocarcinomas: a proteomic approach. Int. J. Cancer. 2008 Mar 3/15/2008; 122(6): 1303-10. PMID: 18000824, DOI: 10.1002/ijc.23258, ISSN: 1097-0215.

Belkhiri A, Dar AA, Zaika A, Kelley M, El-Rifai W. T-Darpp promotes cancer cell survival by up-regulation of Bcl2 through Akt-dependent mechanism. Cancer Res. 2008 Jan 1/15/2008; 68(2): 395-403. PMID: 18199533, PII: 68/2/395, DOI: 10.1158/0008-5472.CAN-07-1580, ISSN: 1538-7445.

Razvi MH, Peng D, Dar AA, Powell SM, Frierson HF, Moskaluk CA, Washington K, El-Rifai W. Transcriptional oncogenomic hot spots in Barrett's adenocarcinomas: serial analysis of gene expression. Genes Chromosomes Cancer. 2007 Oct; 46(10): 914-28. PMID: 17636545, DOI: 10.1002/gcc.20479, ISSN: 1045-2257.

Kuester D, Dar AA, Moskaluk CC, Krueger S, Meyer F, Hartig R, Stolte M, Malfertheiner P, Lippert H, Roessner A, El-Rifai W, Schneider-Stock R. Early involvement of death-associated protein kinase promoter hypermethylation in the carcinogenesis of Barrett's esophageal adenocarcinoma and its association with clinical progression. Neoplasia. 2007 Mar; 9(3): 236-45. PMID: 17401463, PMCID: PMC1838580, ISSN: 1476-5586.

Lee OJ, Hong SM, Razvi MH, Peng D, Powell SM, Smoklin M, Moskaluk CA, El-Rifai W. Expression of calcium-binding proteins S100A2 and S100A4 in Barrett's adenocarcinomas. Neoplasia. 2006 Oct; 8(10): 843-50. PMID: 17032501, PMCID: PMC1715926, DOI: 10.1593/neo.06481, ISSN: 1476-5586.

Tomkova K, El-Rifai W, Vilgelm A, Kelly MC, Wang TC, Zaika AI. The gastrin gene promoter is regulated by p73 isoforms in tumor cells. Oncogene [print-electronic]. 2006 Sep 9/28/2006; 25(44): 6032-6. PMID: 16636659, PII: 1209610, DOI: 10.1038/sj.onc.1209610, ISSN: 0950-9232.

Maqani N, Belkhiri A, Moskaluk C, Knuutila S, Dar AA, El-Rifai W. Molecular dissection of 17q12 amplicon in upper gastrointestinal adenocarcinomas. Mol. Cancer Res. 2006 Jul; 4(7): 449-55. PMID: 16849520, PII: 4/7/449, DOI: 10.1158/1541-7786.MCR-06-0058, ISSN: 1541-7786.

Zaika AI, El-Rifai W. The role of p53 protein family in gastrointestinal malignancies. Cell Death Differ. 2006 Jun; 13(6): 935-40. PMID: 16557273, PII: 4401897, DOI: 10.1038/sj.cdd.4401897, ISSN: 1350-9047.

Wu Z, Siadaty MS, Riddick G, Frierson HF, Lee JK, Golden W, Knuutila S, Hampton GM, El-Rifai W, Theodorescu D. A novel method for gene expression mapping of metastatic competence in human bladder cancer. Neoplasia. 2006 Mar; 8(3): 181-9. PMID: 16611411, PMCID: PMC1578518, DOI: 10.1593/neo.05727, ISSN: 1476-5586.

McChesney PA, Aiyar SE, Lee OJ, Zaika A, Moskaluk C, Li R, El-Rifai W. Cofactor of BRCA1: a novel transcription factor regulator in upper gastrointestinal adenocarcinomas. Cancer Res. 2006 Feb 2/1/2006; 66(3): 1346-53. PMID: 16452188, PII: 66/3/1346, DOI: 10.1158/0008-5472.CAN-05-3593, ISSN: 0008-5472.

Lee OJ, Schneider-Stock R, McChesney PA, Kuester D, Roessner A, Vieth M, Moskaluk CA, El-Rifai W. Hypermethylation and loss of expression of glutathione peroxidase-3 in Barrett's tumorigenesis. Neoplasia. 2005 Sep; 7(9): 854-61. PMID: 16229808, PMCID: PMC1501938, ISSN: 1522-8002.

Belkhiri A, Zaika A, Pidkovka N, Knuutila S, Moskaluk C, El-Rifai W. Darpp-32: a novel antiapoptotic gene in upper gastrointestinal carcinomas. Cancer Res. 2005 Aug 8/1/2005; 65(15): 6583-92. PMID: 16061638, PII: 65/15/6583, DOI: 10.1158/0008-5472.CAN-05-1433, ISSN: 0008-5472.

Johnson AH, Frierson HF, Zaika A, Powell SM, Roche J, Crowe S, Moskaluk CA, El-Rifai W. Expression of tight-junction protein claudin-7 is an early event in gastric tumorigenesis. Am. J. Pathol. 2005 Aug; 167(2): 577-84. PMID: 16049341, PMCID: PMC1603560, PII: S0002-9440(10)62999-9, DOI: 10.1016/S0002-9440(10)62999-9, ISSN: 0002-9440.

Gutiérrez MI, Siraj AK, Khaled H, Koon N, El-Rifai W, Bhatia K. CpG island methylation in Schistosoma- and non-Schistosoma-associated bladder cancer. Mod. Pathol. 2004 Oct; 17(10): 1268-74. PMID: 15154012, PII: 3800177, DOI: 10.1038/modpathol.3800177, ISSN: 0893-3952.

Tomkova K, Belkhiri A, El-Rifai W, Zaika AI. P73 isoforms can induce T-cell factor-dependent transcription in gastrointestinal cells. Cancer Res. 2004 Sep 9/15/2004; 64(18): 6390-3. PMID: 15374945, PII: 64/18/6390, DOI: 10.1158/0008-5472.CAN-04-2176, ISSN: 0008-5472.

Wagner KW, Sapinoso LM, El-Rifai W, Frierson HF, Butz N, Mestan J, Hofmann F, Deveraux QL, Hampton GM. Overexpression, genomic amplification and therapeutic potential of inhibiting the UbcH10 ubiquitin conjugase in human carcinomas of diverse anatomic origin. Oncogene. 2004 Aug 8/26/2004; 23(39): 6621-9. PMID: 15208666, PII: 1207861, DOI: 10.1038/sj.onc.1207861, ISSN: 0950-9232.

Varis A, Zaika A, Puolakkainen P, Nagy B, Madrigal I, Kokkola A, Väyrynen A, Kärkkäinen P, Moskaluk C, El-Rifai W, Knuutila S. Coamplified and overexpressed genes at ERBB2 locus in gastric cancer. Int. J. Cancer. 2004 Apr 4/20/2004; 109(4): 548-53. PMID: 14991576, DOI: 10.1002/ijc.20001, ISSN: 0020-7136.

Koon N, Zaika A, Moskaluk CA, Frierson HF, Knuutila S, Powell SM, El-Rifai W. Clustering of molecular alterations in gastroesophageal carcinomas. Neoplasia. 2004 Mar; 6(2): 143-9. PMID: 15140403, PMCID: PMC1502088, DOI: 10.1593/neo.03385, ISSN: 1522-8002.

Koon N, Schneider-Stock R, Sarlomo-Rikala M, Lasota J, Smolkin M, Petroni G, Zaika A, Boltze C, Meyer F, Andersson L, Knuutila S, Miettinen M, El-Rifai W. Molecular targets for tumour progression in gastrointestinal stromal tumours. Gut. 2004 Feb; 53(2): 235-40. PMID: 14724156, PMCID: PMC1774925, ISSN: 0017-5749.

Beckler AD, Roche JK, Harper JC, Petroni G, Frierson HF, Moskaluk CA, El-Rifai W, Powell SM. Decreased abundance of trefoil factor 1 transcript in the majority of gastric carcinomas. Cancer. 2003 Nov 11/15/2003; 98(10): 2184-91. PMID: 14601088, DOI: 10.1002/cncr.11789, ISSN: 0008-543X.

Beckler A, Moskaluk CA, Zaika A, Hampton GM, Powell SM, Frierson HF, El-Rifai W. Overexpression of the 32-kilodalton dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein in common adenocarcinomas. Cancer. 2003 Oct 10/1/2003; 98(7): 1547-51. PMID: 14508844, DOI: 10.1002/cncr.11654, ISSN: 0008-543X.

Berr SS, Roche JK, El-Rifai W, Smith MF, Powell SM. Magnetic resonance imaging of gastric cancer in Tff1 knock-out mice. Magn Reson Med. 2003 Jun; 49(6): 1033-6. PMID: 12768581, DOI: 10.1002/mrm.10458, ISSN: 0740-3194.

Deng D, El-Rifai W, Ji J, Zhu B, Trampont P, Li J, Smith MF, Powel SM. Hypermethylation of metallothionein-3 CpG island in gastric carcinoma. Carcinogenesis. 2003 Jan; 24(1): 25-9. PMID: 12538345, ISSN: 0143-3334.

El-Rifai W, Moskaluk CA, Abdrabbo MK, Harper J, Yoshida C, Riggins GJ, Frierson HF, Powell SM. Gastric cancers overexpress S100A calcium-binding proteins. Cancer Res. 2002 Dec 12/1/2002; 62(23): 6823-6. PMID: 12460893, ISSN: 0008-5472.

El-Rifai W, Smith MF, Li G, Beckler A, Carl VS, Montgomery E, Knuutila S, Moskaluk CA, Frierson HF, Powell SM. Gastric cancers overexpress DARPP-32 and a novel isoform, t-DARPP. Cancer Res. 2002 Jul 7/15/2002; 62(14): 4061-4. PMID: 12124342, ISSN: 0008-5472.

Miettinen M, El-Rifai W, H L Sobin L, Lasota J. Evaluation of malignancy and prognosis of gastrointestinal stromal tumors: a review. Hum. Pathol. 2002 May; 33(5): 478-83. PMID: 12094372, PII: S0046817702000175, ISSN: 0046-8177.

Dematteo RP, Heinrich MC, El-Rifai WM, Demetri G. Clinical management of gastrointestinal stromal tumors: before and after STI-571. Hum. Pathol. 2002 May; 33(5): 466-77. PMID: 12094371, PII: S0046817702000163, ISSN: 0046-8177.

Varis A, Wolf M, Monni O, Vakkari ML, Kokkola A, Moskaluk C, Frierson H, Powell SM, Knuutila S, Kallioniemi A, El-Rifai W. Targets of gene amplification and overexpression at 17q in gastric cancer. Cancer Res. 2002 May 5/1/2002; 62(9): 2625-9. PMID: 11980659, ISSN: 0008-5472.

el-Rifai W, Powell SM. Molecular and biologic basis of upper gastrointestinal malignancy. Gastric carcinoma. Surg. Oncol. Clin. N. Am. 2002 Apr; 11(2): 273-91, viii. PMID: 12424850, ISSN: 1055-3207.

El-Rifai W, Powell SM. Molecular biology of gastric cancer. Semin Radiat Oncol. 2002 Apr; 12(2): 128-40. PMID: 11979414, PII: S1053-4296(02)80012-1, DOI: 10.1053/srao.2002.30815, ISSN: 1053-4296.

Shuber AP, Ascaño JJ, Boynton KA, Mitchell A, Frierson HF, El-Rifai W, Powell SM. Accurate, noninvasive detection of Helicobacter pylori DNA from stool samples: potential usefulness for monitoring treatment. J. Clin. Microbiol. 2002 Jan; 40(1): 262-4. PMID: 11773127, PMCID: PMC120120, ISSN: 0095-1137.

Ascaño JJ, Frierson H, Moskaluk CA, Harper JC, Roviello F, Jackson CE, El-Rifai W, Vindigni C, Tosi P, Powell SM. Inactivation of the E-cadherin gene in sporadic diffuse-type gastric cancer. Mod. Pathol. 2001 Oct; 14(10): 942-9. PMID: 11598162, DOI: 10.1038/modpathol.3880416, ISSN: 0893-3952.

El-Rifai W, Frierson HF, Moskaluk CA, Harper JC, Petroni GR, Bissonette EA, Jones DR, Knuutila S, Powell SM. Genetic differences between adenocarcinomas arising in Barrett's esophagus and gastric mucosa. Gastroenterology. 2001 Sep; 121(3): 592-8. PMID: 11522743, PII: S0016508501578166, ISSN: 0016-5085.

Tapper J, Kettunen E, El-Rifai W, Seppälä M, Andersson LC, Knuutila S. Changes in gene expression during progression of ovarian carcinoma. Cancer Genet. Cytogenet. 2001 Jul 7/1/2001; 128(1): 1-6. PMID: 11454421, PII: S0165460801003867, ISSN: 0165-4608.

El-Rifai W, Frierson HF, Harper JC, Powell SM, Knuutila S. Expression profiling of gastric adenocarcinoma using cDNA array. Int. J. Cancer. 2001 Jun 6/15/2001; 92(6): 832-8. PMID: 11351303, PII: 10.1002/ijc.1264, DOI: 10.1002/ijc.1264, ISSN: 0020-7136.

El-Rifai W, Rutherford S, Knuutila S, Frierson HF, Moskaluk CA. Novel DNA copy number losses in chromosome 12q12--q13 in adenoid cystic carcinoma. Neoplasia. 2001 May; 3(3): 173-8. PMID: 11494110, PMCID: PMC1505590, DOI: 10.1038/sj/neo/7900158, ISSN: 1522-8002.

Kettunen E, Nissén AM, Ollikainen T, Taavitsainen M, Tapper J, Mattson K, Linnainmaa K, Knuutila S, El-Rifai W. Gene expression profiling of malignant mesothelioma cell lines: cDNA array study. Int. J. Cancer. 2001 Feb 2/15/2001; 91(4): 492-6. PMID: 11251971, PII: 10.1002/1097-0215(200002)9999:9999<::AID-IJC1094>3.0.CO;2-M, ISSN: 0020-7136.

Kiuru-Kuhlefelt S, El-Rifai W, Fanburg-Smith J, Kere J, Miettinen M, Knuutila S. Concomitant DNA copy number amplification at 17q and 22q in dermatofibrosarcoma protuberans. Cytogenet. Cell Genet. 2001; 92(3-4): 192-5. PMID: 11435686, PII: 56901, DOI: 56901, ISSN: 0301-0171.

Wolf M, El-Rifai W, Tarkkanen M, Kononen J, Serra M, Eriksen EF, Elomaa I, Kallioniemi A, Kallioniemi OP, Knuutila S. Novel findings in gene expression detected in human osteosarcoma by cDNA microarray. Cancer Genet. Cytogenet. 2000 Dec; 123(2): 128-32. PMID: 11156738, PII: S0165-4608(00)00319-8, ISSN: 0165-4608.

El-Rifai W, Sarlomo-Rikala M, Andersson LC, Knuutila S, Miettinen M. DNA sequence copy number changes in gastrointestinal stromal tumors: tumor progression and prognostic significance. Cancer Res. 2000 Jul 7/15/2000; 60(14): 3899-903. PMID: 10919666, ISSN: 0008-5472.

El-Rifai W, Sarlomo-Rikala M, Andersson LC, Miettinen M, Knuutila S. High-resolution deletion mapping of chromosome 14 in stromal tumors of the gastrointestinal tract suggests two distinct tumor suppressor loci. Genes Chromosomes Cancer. 2000 Apr; 27(4): 387-91. PMID: 10719369, PII: 10.1002/(SICI)1098-2264(200004)27:4<387::AID-GCC8>3.0.CO;2-C, ISSN: 1045-2257.

El-Rifai W, Kamel D, Larramendy ML, Shoman S, Gad Y, Baithun S, El-Awady M, Eissa S, Khaled H, Soloneski S, Sheaff M, Knuutila S. DNA copy number changes in Schistosoma-associated and non-Schistosoma-associated bladder cancer. Am. J. Pathol. 2000 Mar; 156(3): 871-8. PMID: 10702404, PMCID: PMC1876852, PII: S0002-9440(10)64956-5, DOI: 10.1016/S0002-9440(10)64956-5, ISSN: 0002-9440.

Kettunen E, el-Rifai W, Björkqvist AM, Wolff H, Karjalainen A, Anttila S, Mattson K, Husgafvel-Pursiainen K, Knuutila S. A broad amplification pattern at 3q in squamous cell lung cancer--a fluorescence in situ hybridization study. Cancer Genet. Cytogenet. 2000 Feb; 117(1): 66-70. PMID: 10700870, PII: S0165-4608(99)00146-6, ISSN: 0165-4608.

Knuutila S, Aalto Y, Autio K, Björkqvist AM, El-Rifai W, Hemmer S, Huhta T, Kettunen E, Kiuru-Kuhlefelt S, Larramendy ML, Lushnikova T, Monni O, Pere H, Tapper J, Tarkkanen M, Varis A, Wasenius VM, Wolf M, Zhu Y. DNA copy number losses in human neoplasms. Am. J. Pathol. 1999 Sep; 155(3): 683-94. PMID: 10487825, PMCID: PMC1866903, PII: S0002-9440(10)65166-8, DOI: 10.1016/S0002-9440(10)65166-8, ISSN: 0002-9440.

el-Rifai W, Tarmo L, Hemmer S, Försti A, Pedersen N, Lichtenstein P, Ahlbom A, Söderberg M, Knuutila S, Hemminki K. DNA copy number losses at 1p32-pter in monozygotic twins concordant for breast cancer. Cancer Genet. Cytogenet. 1999 Jul 7/15/1999; 112(2): 169-72. PMID: 10686947, PII: S016546089800274X, ISSN: 0165-4608.

Plass C, Yu F, Yu L, Strout MP, El-Rifai W, Elonen E, Knuutila S, Marcucci G, Young DC, Held WA, Bloomfield CD, Caligiuri MA. Restriction landmark genome scanning for aberrant methylation in primary refractory and relapsed acute myeloid leukemia; involvement of the WIT-1 gene. Oncogene. 1999 May 5/20/1999; 18(20): 3159-65. PMID: 10340388, DOI: 10.1038/sj.onc.1202651, ISSN: 0950-9232.

Zhu Y, Monni O, El-Rifai W, Siitonen SM, Vilpo L, Vilpo J, Knuutila S. Discontinuous deletions at 11q23 in B cell chronic lymphocytic leukemia. Leukemia. 1999 May; 13(5): 708-12. PMID: 10374874, ISSN: 0887-6924.

Larramendy ML, el-Rifai W, Kokkola A, Puolakkainen P, Monni O, Salovaara R, Aarnio M, Knuutila S. Comparative genomic hybridization reveals differences in DNA copy number changes between sporadic gastric carcinomas and gastric carcinomas from patients with hereditary nonpolyposis colorectal cancer. Cancer Genet. Cytogenet. 1998 Oct 10/1/1998; 106(1): 62-5. PMID: 9772911, PII: S0165460898000466, ISSN: 0165-4608.

Larramendy ML, Huhta T, Vettenranta K, El-Rifai W, Lundin J, Pakkala S, Saarinen-Pihkala UM, Knuutila S. Comparative genomic hybridization in childhood acute lymphoblastic leukemia. Leukemia. 1998 Oct; 12(10): 1638-44. PMID: 9766511, ISSN: 0887-6924.

El-Rifai W, Sarlomo-Rikala M, Knuutila S, Miettinen M. DNA copy number changes in development and progression in leiomyosarcomas of soft tissues. Am. J. Pathol. 1998 Sep; 153(3): 985-90. PMID: 9736047, PMCID: PMC1853026, PII: S0002-9440(10)65640-4, DOI: 10.1016/S0002-9440(10)65640-4, ISSN: 0002-9440.

Sarlomo-Rikala M, El-Rifai W, Lahtinen T, Andersson LC, Miettinen M, Knuutila S. Different patterns of DNA copy number changes in gastrointestinal stromal tumors, leiomyomas, and schwannomas. Hum. Pathol. 1998 May; 29(5): 476-81. PMID: 9596271, ISSN: 0046-8177.

Knuutila S, Björkqvist AM, Autio K, Tarkkanen M, Wolf M, Monni O, Szymanska J, Larramendy ML, Tapper J, Pere H, El-Rifai W, Hemmer S, Wasenius VM, Vidgren V, Zhu Y. DNA copy number amplifications in human neoplasms: review of comparative genomic hybridization studies. Am. J. Pathol. 1998 May; 152(5): 1107-23. PMID: 9588877, PMCID: PMC1858578, ISSN: 0002-9440.

Kiuru-Kuhlefelt S, El-Rifai W, Sarlomo-Rikala M, Knuutila S, Miettinen M. DNA copy number changes in alveolar soft part sarcoma: a comparative genomic hybridization study. Mod. Pathol. 1998 Mar; 11(3): 227-31. PMID: 9521467, ISSN: 0893-3952.

Larramendy ML, El-Rifai W, Knuutila S. Comparison of fluorescein isothiocyanate- and Texas red-conjugated nucleotides for direct labeling in comparative genomic hybridization. Cytometry. 1998 Mar 3/1/1998; 31(3): 174-9. PMID: 9515716, PII: 10.1002/(SICI)1097-0320(19980301)31:3<174::AID-CYTO4>3.0.CO;2-L, ISSN: 0196-4763.

el-Rifai W, Sarlomo-Rikala M, Andersson LC, Miettinen M, Knuutila S. DNA copy number changes in gastrointestinal stromal tumors--a distinct genetic entity. Ann Chir Gynaecol. 1998; 87(4): 287-90. PMID: 9891767, ISSN: 0355-9521.

El-Rifai W, Harper JC, Cummings OW, Hyytinen ER, Frierson HF, Knuutila S, Powell SM. Consistent genetic alterations in xenografts of proximal stomach and gastro-esophageal junction adenocarcinomas. Cancer Res. 1998 Jan 1/1/1998; 58(1): 34-7. PMID: 9426053, ISSN: 0008-5472.

Knuutila S, Armengol G, Björkqvist AM, el-Rifai W, Larramendy ML, Monni O, Szymanska J. Comparative genomic hybridization study on pooled DNAs from tumors of one clinical-pathological entity. Cancer Genet. Cytogenet. 1998 Jan 1/1/1998; 100(1): 25-30. PMID: 9406576, PII: S0165460897000010, ISSN: 0165-4608.

Miettinen MM, el-Rifai W, Sarlomo-Rikala M, Andersson LC, Knuutila S. Tumor size-related DNA copy number changes occur in solitary fibrous tumors but not in hemangiopericytomas. Mod. Pathol. 1997 Dec; 10(12): 1194-200. PMID: 9436963, ISSN: 0893-3952.

el-Rifai W, Larramendy ML, Björkqvist AM, Hemmer S, Knuutila S. Optimization of comparative genomic hybridization using fluorochrome conjugated to dCTP and dUTP nucleotides. Lab. Invest. 1997 Dec; 77(6): 699-700. PMID: 9426409, ISSN: 0023-6837.

El-Rifai W, Elonen E, Larramendy M, Ruutu T, Knuutila S. Chromosomal breakpoints and changes in DNA copy number in refractory acute myeloid leukemia. Leukemia. 1997 Jul; 11(7): 958-63. PMID: 9204975, ISSN: 0887-6924.

El-Rifai W, Ruutu T, Vettenranta K, Elonen E, Saarinen UM, Volin L, Knuutila S. Follow-up of residual disease using metaphase-FISH in patients with acute lymphoblastic leukemia in remission. Leukemia. 1997 May; 11(5): 633-8. PMID: 9180284, ISSN: 0887-6924.

El-Rifai W, Ruutu T, Elonen E, Volin L, Knuutila S. Prognostic value of metaphase-fluorescence in situ hybridization in follow-up of patients with acute myeloid leukemia in remission. Blood. 1997 May 5/1/1997; 89(9): 3330-4. PMID: 9129039, ISSN: 0006-4971.

Sulisalo T, Mäkitie O, Sistonen P, Ridanpää M, el-Rifai W, Ruuskanen O, de la Chapelle A, Kaitila I. Uniparental disomy in cartilage-hair hypoplasia. Eur. J. Hum. Genet. 1997 Jan; 5(1): 35-42. PMID: 9156319, ISSN: 1018-4813.

Billström R, Johansson B, Strömbeck B, el-Rifai W, Larramendy M, Olofsson T, Mitelman F, Knuutila S. Clonal CD5-positive B lymphocytes in myelodysplastic syndrome with systemic vasculitis and trisomy 8. Ann. Hematol. 1997 Jan; 74(1): 37-40. PMID: 9031614, ISSN: 0939-5555.

Mertens F, Pålsson E, Lindstrand A, Toksvig-Larsen S, Knuutila S, Larramendy ML, el-Rifai W, Limon J, Mitelman F, Mandahl N. Evidence of somatic mutations in osteoarthritis. Hum. Genet. 1996 Dec; 98(6): 651-6. PMID: 8931694, ISSN: 0340-6717.

el-Rifai W, Sarlomo-Rikala M, Miettinen M, Knuutila S, Andersson LC. DNA copy number losses in chromosome 14: an early change in gastrointestinal stromal tumors. Cancer Res. 1996 Jul 7/15/1996; 56(14): 3230-3. PMID: 8764113, ISSN: 0008-5472.

Slunga-Tallberg A, el-Rifai W, Keinänen M, Ylinen K, Kurki T, Klinger K, Ylikorkala O, Larramendy ML, Knuutila S. Maternal origin of transferrin receptor positive cells in venous blood of pregnant women. Clin. Genet. 1996 Apr; 49(4): 196-9. PMID: 8828985, ISSN: 0009-9163.

el-Rifai W, Ruutu T, Vettenranta K, Temtamy S, Knuutila S. Minimal residual disease after allogeneic bone marrow transplantation for chronic myeloid leukaemia: a metaphase-FISH study. Br. J. Haematol. 1996 Feb; 92(2): 365-9. PMID: 8603001, ISSN: 0007-1048.

El-Rifai W, Larramendy ML, Ruutu T, Knuutila S. Lymphoid involvement in a patient with acute myeloid leukemia: a direct phenotypic and genotypic study of single cells. Genes Chromosomes Cancer. 1996 Jan; 15(1): 34-7. PMID: 8824723, PII: 10.1002/(SICI)1098-2264(199601)15:1<34::AID-GCC5>3.0.CO;2-5, DOI: 10.1002/(SICI)1098-2264(199601)15:1<34::AID-GCC5>3.0.CO;2-5, ISSN: 1045-2257.

el Rifai W, Knuutila S. Fluorescent in situ hybridization on archival G-banded slides. Cytogenet. Cell Genet. 1996; 73(4): 322-4. PMID: 8751387, ISSN: 0301-0171.

Larramendy M, el-Rifai W, Saarinen U, Alitalo R, Luomahaara S, Knuutila S. Myeloid lineage involvement in acute lymphoblastic leukemia: a morphology antibody chromosomes (MAC) study. Exp. Hematol. 1995 Dec; 23(14): 1563-7. PMID: 8542947, ISSN: 0301-472X.

Slunga-Tallberg A, el-Rifai W, Keinänen M, Ylinen K, Kurki T, Klinger K, Ylikorkala O, Knuutila S. Maternal origin of nucleated erythrocytes in peripheral venous blood of pregnant women. Hum. Genet. 1995 Jul; 96(1): 53-7. PMID: 7607654, ISSN: 0340-6717.

Knuutila S, Larramendy ML, Elfving P, el-Rifai W, Miettinen A, Mitelman F. Trisomy 7 in non-neoplastic tubular epithelial cells of the kidney. Hum. Genet. 1995 Feb; 95(2): 149-56. PMID: 7860060, ISSN: 0340-6717.

el-Rifai W, Leisti J, Kähkönen M, Pietarinen A, Altherr MR, Knuutila S. A patient with Wolf-Hirschhorn syndrome originating from translocation t(4;8) (p16.3;q24.3)pat. J. Med. Genet. 1995 Jan; 32(1): 65-7. PMID: 7897631, PMCID: PMC1050183, ISSN: 0022-2593.

el-Rifai W, Pettersson T, Larramendy ML, Knuutila S. Lineage involvement and karyotype in a patient with myelodysplasia and blood basophilia. Eur. J. Haematol. 1994 Nov; 53(5): 288-92. PMID: 7813709, ISSN: 0902-4441.

Available Postdoctoral Position Details
Posted: 1/29/2016
A post-doctoral position is available in Dr. El-Rifai Laboratory, laboratories of Surgical Oncology and Cancer Biology. The laboratory is well equipped with access to outstanding core facilities at the University of Vanderbilt School of Medicine and Cancer Center. University of Vanderbilt School of Medicine is one of the top academic institutes in the US.
This position is to support ongoing work to investigate mechanisms of antioxidant response and redox regulation in gastric and esophageal tumorigenesis. We are highly interested in understanding the role of these mechanisms in sensitivity and resistance to conventional and targeted therapies in human cancers. Applicants should be highly motivated individuals who have a PhD and/or MD degree, extensive experience in molecular biology, and interest in cancer biology.

A link to El-Rifai lab publications:


Candidates with publications in peer-reviewed journals are strongly encouraged to apply. Send curriculum vitae, statement of research interest, and names and contact information of at least two references.