James E. Crowe, Jr., M.D.

Professor

james.crowe@vanderbilt.edu

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Faculty Appointments
Professor of Pediatrics Ann Scott Carell ChairProfessor of Pathology, Microbiology and Immunology
Education
M.D., Medicine, University of North Carolina, Chapel Hill, North CarolinaB.S., Premedicine, Davidson College, Davidson, North Carolina
Office Address
11475 MRB IV
2213 Garland Ave
Nashville, TN 37232-0417
Research Description
Our laboratory studies focus on the molecular basis for the development of effective B cell responses to viruses in humans. We also have active programs in biodefense research, vaccine and monoclonal antibody development, and biotechnology. Our group uses a very broad array of techniques including molecular and cellular biology, state-of-the-art imaging and flow cytometry, bioinformatics, next generation sequencing, EM and crystallography, computational modeling, and bioengineering approaches to attack the scientific problems that are of interest to us. Our philosophy is to work on major human pathogens, not model systems. Current studies center on Ebola and Marburg, chikungunya, Zika, influenza, dengue viruses, HIV, respiratory syncytial virus, human metapneumovirus, rotavirus and noroviruses, rabies, Rift Valley Fever virus, West Nile virus, and vaccinia virus/monkeypox/smallpox. We also have new studies of the human antibody response to bacterial toxins including those of pertussis, Staph aureus and C difficile.

Our basic science studies focus on the genetic origins and structural determinants of virus neutralizing antibodies. To study this area, we have developed unique B cell and antibody engineering techniques including novel methods for generation of human B cell hybridomas, direct cloning of antibody variable genes from virus-specific B cells, high throughput sequence analysis, and full-length expression of recombinant antibodies including high throughput expression techniques. This work has allowed us to understand better structure/function characteristics of antiviral antibodies. Several of the recombinant human antibodies that we have generated are being developed commercially for testing in humans.

Research Keywords
Monoclonal antibodies, Immunology, Infectious disease, Microbiology, Viruses, B cells, influenza virus, dengue virus, HIV, RSV, MPV, rotavirus, smallpox, monkeypox virus vaccinia virus, Ebola virus, Marburg virus, chikungunya virus, Zika virus, respiratory syncytial virus, human metapneumovirus, norovirus, rabies virus, Rift Valley Fever virus, West Nile virus. Pertussis. Staphylococcus aureus, Clostridium difficile
Publications
Hicar MD, Chen X, Kalams SA, Sojar H, Landucci G, Forthal DN, Spearman P, Crowe JE. Low frequency of broadly neutralizing HIV antibodies during chronic infection even in quaternary epitope targeting antibodies containing large numbers of somatic mutations. Mol. Immunol [print-electronic]. 2015 Dec 12/31/2015; 70: 94-103. PMID: 26748387, PII: S0161-5890(15)30125-5, DOI: 10.1016/j.molimm.2015.12.002, ISSN: 1872-9142.

Fox JM, Long F, Edeling MA, Lin H, van Duijl-Richter MK, Fong RH, Kahle KM, Smit JM, Jin J, Simmons G, Doranz BJ, Crowe JE, Fremont DH, Rossmann MG, Diamond MS. Broadly Neutralizing Alphavirus Antibodies Bind an Epitope on E2 and Inhibit Entry and Egress. Cell [print-electronic]. 2015 Nov 11/19/2015; 163(5): 1095-107. PMID: 26553503, PMCID: PMC4659373, PII: S0092-8674(15)01406-3, DOI: 10.1016/j.cell.2015.10.050, ISSN: 1097-4172.

Long F, Fong RH, Austin SK, Chen Z, Klose T, Fokine A, Liu Y, Porta J, Sapparapu G, Akahata W, Doranz BJ, Crowe JE, Diamond MS, Rossmann MG. Cryo-EM structures elucidate neutralizing mechanisms of anti-chikungunya human monoclonal antibodies with therapeutic activity. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2015 Nov 11/10/2015; 112(45): 13898-903. PMID: 26504196, PMCID: PMC4653152, PII: 1515558112, DOI: 10.1073/pnas.1515558112, ISSN: 1091-6490.

Crowe JE, Koff WC. Deciphering the human immunome. Expert Rev Vaccines [print-electronic]. 2015 Nov; 14(11): 1421-5. PMID: 26485213, DOI: 10.1586/14760584.2015.1082427, ISSN: 1744-8395.

Winarski KL, Thornburg NJ, Yu Y, Sapparapu G, Crowe JE, Spiller BW. Vaccine-elicited antibody that neutralizes H5N1 influenza and variants binds the receptor site and polymorphic sites. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2015 Jul 7/28/2015; 112(30): 9346-51. PMID: 26170302, PMCID: PMC4522792, PII: 1502762112, DOI: 10.1073/pnas.1502762112, ISSN: 1091-6490.

Callaway JB, Smith SA, McKinnon KP, de Silva AM, Crowe JE, Ting JP. Spleen Tyrosine Kinase (Syk) Mediates IL-1ß Induction by Primary Human Monocytes during Antibody-enhanced Dengue Virus Infection. J. Biol. Chem [print-electronic]. 2015 Jul 7/10/2015; 290(28): 17306-20. PMID: 26032420, PMCID: PMC4498069, PII: M115.664136, DOI: 10.1074/jbc.M115.664136, ISSN: 1083-351X.

Smith SA, Silva LA, Fox JM, Flyak AI, Kose N, Sapparapu G, Khomandiak S, Ashbrook AW, Kahle KM, Fong RH, Swayne S, Doranz BJ, McGee CE, Heise MT, Pal P, Brien JD, Austin SK, Diamond MS, Dermody TS, Crowe JE. Isolation and Characterization of Broad and Ultrapotent Human Monoclonal Antibodies with Therapeutic Activity against Chikungunya Virus. Cell Host Microbe. 2015 Jul 7/8/2015; 18(1): 86-95. PMID: 26159721, PMCID: PMC4501771, PII: S1931-3128(15)00257-7, DOI: 10.1016/j.chom.2015.06.009, ISSN: 1934-6069.

Fibriansah G, Ibarra KD, Ng TS, Smith SA, Tan JL, Lim XN, Ooi JS, Kostyuchenko VA, Wang J, de Silva AM, Harris E, Crowe JE, Lok SM. DENGUE VIRUS. Cryo-EM structure of an antibody that neutralizes dengue virus type 2 by locking E protein dimers. Science. 2015 Jul 7/3/2015; 349(6243): 88-91. PMID: 26138979, PMCID: PMC4672004, PII: 349/6243/88, DOI: 10.1126/science.aaa8651, ISSN: 1095-9203.

Sevy AM, Jacobs TM, Crowe JE, Meiler J. Design of Protein Multi-specificity Using an Independent Sequence Search Reduces the Barrier to Low Energy Sequences. PLoS Comput. Biol. 2015 Jul; 11(7): e1004300. PMID: 26147100, PMCID: PMC4493036, PII: PCOMPBIOL-D-15-00033, DOI: 10.1371/journal.pcbi.1004300, ISSN: 1553-7358.

Hooper KA, Crowe JE, Bloom JD. Influenza viruses with receptor-binding N1 neuraminidases occur sporadically in several lineages and show no attenuation in cell culture or mice. J. Virol [print-electronic]. 2015 Apr; 89(7): 3737-45. PMID: 25609803, PMCID: PMC4403400, PII: JVI.00012-15, DOI: 10.1128/JVI.00012-15, ISSN: 1098-5514.

Simões EA, DeVincenzo JP, Boeckh M, Bont L, Crowe JE, Griffiths P, Hayden FG, Hodinka RL, Smyth RL, Spencer K, Thirstrup S, Walsh EE, Whitley RJ. Challenges and opportunities in developing respiratory syncytial virus therapeutics. J. Infect. Dis. 2015 Mar 3/15/2015; 211 Suppl 1: S1-S20. PMID: 25713060, PMCID: PMC4345819, PII: jiu828, DOI: 10.1093/infdis/jiu828, ISSN: 1537-6613.

Hashiguchi T, Fusco ML, Bornholdt ZA, Lee JE, Flyak AI, Matsuoka R, Kohda D, Yanagi Y, Hammel M, Crowe JE, Saphire EO. Structural basis for Marburg virus neutralization by a cross-reactive human antibody. Cell. 2015 Feb 2/26/2015; 160(5): 904-12. PMID: 25723165, PMCID: PMC4344967, PII: S0092-8674(15)00119-1, DOI: 10.1016/j.cell.2015.01.041, ISSN: 1097-4172.

Flyak AI, Ilinykh PA, Murin CD, Garron T, Shen X, Fusco ML, Hashiguchi T, Bornholdt ZA, Slaughter JC, Sapparapu G, Klages C, Ksiazek TG, Ward AB, Saphire EO, Bukreyev A, Crowe JE. Mechanism of human antibody-mediated neutralization of Marburg virus. Cell. 2015 Feb 2/26/2015; 160(5): 893-903. PMID: 25723164, PMCID: PMC4344968, PII: S0092-8674(15)00074-4, DOI: 10.1016/j.cell.2015.01.031, ISSN: 1097-4172.

Smith SA, Nivarthi UK, de Alwis R, Kose N, Sapparapu G, Bombardi R, Kahle KM, Pfaff JM, Lieberman S, Doranz BJ, de Silva AM, Crowe JE. Dengue Virus prM-Specific Human Monoclonal Antibodies with Virus Replication-Enhancing Properties Recognize a Single Immunodominant Antigenic Site. J. Virol. 2015; 90(2): 780-9. PMID: 26512092, PII: JVI.01805-15, DOI: 10.1128/JVI.01805-15, ISSN: 1098-5514.

Gallichotte EN, Widman DG, Yount BL, Wahala WM, Durbin A, Whitehead S, Sariol CA, Crowe JE, de Silva AM, Baric RS. A new quaternary structure epitope on dengue virus serotype 2 is the target of durable type-specific neutralizing antibodies. MBio. 2015; 6(5): e01461-15. PMID: 26463165, PMCID: PMC4620467, PII: mBio.01461-15, DOI: 10.1128/mBio.01461-15, ISSN: 2150-7511.

Callaway JB, Smith SA, Widman DG, McKinnon KP, Scholle F, Sempowski GD, Dittmer DP, Crowe JE, de Silva AM, Ting JP. Source and Purity of Dengue-Viral Preparations Impact Requirement for Enhancing Antibody to Induce Elevated IL-1ß Secretion: A Primary Human Monocyte Model. PLoS ONE. 2015; 10(8): e0136708. PMID: 26301593, PMCID: PMC4547738, PII: PONE-D-14-32063, DOI: 10.1371/journal.pone.0136708, ISSN: 1932-6203.

Chukwuma VU, Hicar MD, Chen X, Nicholas KJ, Joyner A, Kalams SA, Landucci G, Forthal DN, Spearman PW, Crowe JE. Association of VH4-59 Antibody Variable Gene Usage with Recognition of an Immunodominant Epitope on the HIV-1 Gag Protein. PLoS ONE. 2015; 10(7): e0133509. PMID: 26226263, PMCID: PMC4520566, PII: PONE-D-15-16451, DOI: 10.1371/journal.pone.0133509, ISSN: 1932-6203.

Fibriansah G, Tan JL, Smith SA, de Alwis R, Ng TS, Kostyuchenko VA, Jadi RS, Kukkaro P, de Silva AM, Crowe JE, Lok SM. A highly potent human antibody neutralizes dengue virus serotype 3 by binding across three surface proteins. Nat Commun. 2015; 6: 6341. PMID: 25698059, PMCID: PMC4346626, PII: ncomms7341, DOI: 10.1038/ncomms7341, ISSN: 2041-1723.

Hotard AL, Lee S, Currier MG, Crowe JE, Sakamoto K, Newcomb DC, Peebles RS, Plemper RK, Moore ML. Identification of residues in the human respiratory syncytial virus fusion protein that modulate fusion activity and pathogenesis. J. Virol [print-electronic]. 2015 Jan; 89(1): 512-22. PMID: 25339762, PMCID: PMC4301159, PII: JVI.02472-14, DOI: 10.1128/JVI.02472-14, ISSN: 1098-5514.

Smith SA, de Alwis AR, Kose N, Jadi RS, de Silva AM, Crowe JE. Isolation of dengue virus-specific memory B cells with live virus antigen from human subjects following natural infection reveals the presence of diverse novel functional groups of antibody clones. J. Virol [print-electronic]. 2014 Nov; 88(21): 12233-41. PMID: 25100837, PMCID: PMC4248927, PII: JVI.00247-14, DOI: 10.1128/JVI.00247-14, ISSN: 1098-5514.

de Alwis R, Williams KL, Schmid MA, Lai CY, Patel B, Smith SA, Crowe JE, Wang WK, Harris E, de Silva AM. Dengue viruses are enhanced by distinct populations of serotype cross-reactive antibodies in human immune sera. PLoS Pathog. 2014 Oct; 10(10): e1004386. PMID: 25275316, PMCID: PMC4183589, PII: PPATHOGENS-D-14-00771, DOI: 10.1371/journal.ppat.1004386, ISSN: 1553-7374.

Dulek DE, Newcomb DC, Toki S, Goliniewska K, Cephus J, Reiss S, Bates JT, Crowe JE, Boyd KL, Moore ML, Zhou W, Peebles RS. STAT4 deficiency fails to induce lung Th2 or Th17 immunity following primary or secondary respiratory syncytial virus (RSV) challenge but enhances the lung RSV-specific CD8+ T cell immune response to secondary challenge. J. Virol [print-electronic]. 2014 Sep 9/1/2014; 88(17): 9655-72. PMID: 24920804, PMCID: PMC4136353, PII: JVI.03299-13, DOI: 10.1128/JVI.03299-13, ISSN: 1098-5514.

Sapparapu G, Sims AL, Aiyegbo MS, Shaikh FY, Harth EM, Crowe JE. Intracellular neutralization of a virus using a cell-penetrating molecular transporter. Nanomedicine (Lond) [print-electronic]. 2014 Aug; 9(11): 1613-24. PMID: 24195674, DOI: 10.2217/nnm.13.139, ISSN: 1748-6963.

Almagro JC, Gilliland GL, Breden F, Scott JK, Sok D, Pauthner M, Reichert JM, Helguera G, Andrabi R, Mabry R, Bléry M, Voss JE, Laurén J, Abuqayyas L, Barghorn S, Ben-Jacob E, Crowe JE, Huston JS, Johnston SA, Krauland E, Lund-Johansen F, Marasco WA, Parren PW, Xu KY. Antibody engineering and therapeutics, The Annual Meeting of the Antibody Society: December 8-12, 2013, Huntington Beach, CA. MAbs [print-electronic]. 2014 May; 6(3): 577-618. PMID: 24589717, PMCID: PMC4011904, PII: 28421, DOI: 10.4161/mabs.28421, ISSN: 1942-0870.

Bates JT, Keefer CJ, Slaughter JC, Kulp DW, Schief WR, Crowe JE. Escape from neutralization by the respiratory syncytial virus-specific neutralizing monoclonal antibody palivizumab is driven by changes in on-rate of binding to the fusion protein. Virology [print-electronic]. 2014 Apr; 454-455: 139-44. PMID: 24725940, PMCID: PMC4004766, PII: S0042-6822(14)00054-3, DOI: 10.1016/j.virol.2014.02.010, ISSN: 1096-0341.

Correia BE, Bates JT, Loomis RJ, Baneyx G, Carrico C, Jardine JG, Rupert P, Correnti C, Kalyuzhniy O, Vittal V, Connell MJ, Stevens E, Schroeter A, Chen M, Macpherson S, Serra AM, Adachi Y, Holmes MA, Li Y, Klevit RE, Graham BS, Wyatt RT, Baker D, Strong RK, Crowe JE, Johnson PR, Schief WR. Proof of principle for epitope-focused vaccine design. Nature [print-electronic]. 2014 Mar 3/13/2014; 507(7491): 201-6. PMID: 24499818, PMCID: PMC4260937, PII: nature12966, DOI: 10.1038/nature12966, ISSN: 1476-4687.

Fibriansah G, Tan JL, Smith SA, de Alwis AR, Ng TS, Kostyuchenko VA, Ibarra KD, Wang J, Harris E, de Silva A, Crowe JE, Lok SM. A potent anti-dengue human antibody preferentially recognizes the conformation of E protein monomers assembled on the virus surface. EMBO Mol Med [print-electronic]. 2014 Mar; 6(3): 358-71. PMID: 24421336, PMCID: PMC3958310, PII: emmm.201303404, DOI: 10.1002/emmm.201303404, ISSN: 1757-4684.

Messer WB, de Alwis R, Yount BL, Royal SR, Huynh JP, Smith SA, Crowe JE, Doranz BJ, Kahle KM, Pfaff JM, White LJ, Sariol CA, de Silva AM, Baric RS. Dengue virus envelope protein domain I/II hinge determines long-lived serotype-specific dengue immunity. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2014 Feb 2/4/2014; 111(5): 1939-44. PMID: 24385585, PMCID: PMC3918811, PII: 1317350111, DOI: 10.1073/pnas.1317350111, ISSN: 1091-6490.

Alonas E, Lifland AW, Gudheti M, Vanover D, Jung J, Zurla C, Kirschman J, Fiore VF, Douglas A, Barker TH, Yi H, Wright ER, Crowe JE, Santangelo PJ. Combining single RNA sensitive probes with subdiffraction-limited and live-cell imaging enables the characterization of virus dynamics in cells. ACS Nano [print-electronic]. 2014 Jan 1/28/2014; 8(1): 302-15. PMID: 24351207, PMCID: PMC3906890, DOI: 10.1021/nn405998v, ISSN: 1936-086X.

Aiyegbo MS, Eli IM, Spiller BW, Williams DR, Kim R, Lee DE, Liu T, Li S, Stewart PL, Crowe JE. Differential accessibility of a rotavirus VP6 epitope in trimers comprising type I, II, or III channels as revealed by binding of a human rotavirus VP6-specific antibody. J. Virol [print-electronic]. 2014 Jan; 88(1): 469-76. PMID: 24155406, PMCID: PMC3911710, PII: JVI.01665-13, DOI: 10.1128/JVI.01665-13, ISSN: 1098-5514.

Briney BS, Willis JR, Finn JA, McKinney BA, Crowe JE. Tissue-specific expressed antibody variable gene repertoires. PLoS ONE. 2014; 9(6): e100839. PMID: 24956460, PMCID: PMC4067404, PII: PONE-D-13-41903, DOI: 10.1371/journal.pone.0100839, ISSN: 1932-6203.

Hong M, Lee PS, Hoffman RM, Zhu X, Krause JC, Laursen NS, Yoon SI, Song L, Tussey L, Crowe JE, Ward AB, Wilson IA. Antibody recognition of the pandemic H1N1 Influenza virus hemagglutinin receptor binding site. J. Virol [print-electronic]. 2013 Nov; 87(22): 12471-80. PMID: 24027321, PMCID: PMC3807900, PII: JVI.01388-13, DOI: 10.1128/JVI.01388-13, ISSN: 1098-5514.

Finn JA, Crowe JE. Impact of new sequencing technologies on studies of the human B cell repertoire. Curr. Opin. Immunol [print-electronic]. 2013 Oct; 25(5): 613-8. PMID: 24161653, PMCID: PMC3882336, PII: S0952-7915(13)00145-3, DOI: 10.1016/j.coi.2013.09.010, ISSN: 1879-0372.

Munden MM, Williams JL, Zhang W, Crowe JE, Munden RF, Cisek LJ. Intermittent testicular torsion in the pediatric patient: sonographic indicators of a difficult diagnosis. AJR Am J Roentgenol. 2013 Oct; 201(4): 912-8. PMID: 24059384, DOI: 10.2214/AJR.12.9448, ISSN: 1546-3141.

Thornburg NJ, Nannemann DP, Blum DL, Belser JA, Tumpey TM, Deshpande S, Fritz GA, Sapparapu G, Krause JC, Lee JH, Ward AB, Lee DE, Li S, Winarski KL, Spiller BW, Meiler J, Crowe JE. Human antibodies that neutralize respiratory droplet transmissible H5N1 influenza viruses. J. Clin. Invest [print-electronic]. 2013 Oct; 123(10): 4405-9. PMID: 23999429, PMCID: PMC3784541, PII: 69377, DOI: 10.1172/JCI69377, ISSN: 1558-8238.

Crowe JE. Universal flu vaccines: primum non nocere. Sci Transl Med. 2013 Aug 8/28/2013; 5(200): 200fs34. PMID: 23986396, PII: 5/200/200fs34, DOI: 10.1126/scitranslmed.3007118, ISSN: 1946-6242.

Stone JW, Thornburg NJ, Blum DL, Kuhn SJ, Wright DW, Crowe JE. Gold nanorod vaccine for respiratory syncytial virus. Nanotechnology [print-electronic]. 2013 Jul 7/26/2013; 24(29): 295102. PMID: 23799651, PMCID: PMC3754908, DOI: 10.1088/0957-4484/24/29/295102, ISSN: 1361-6528.

Smith SA, de Alwis R, Kose N, Durbin AP, Whitehead SS, de Silva AM, Crowe JE. Human monoclonal antibodies derived from memory B cells following live attenuated dengue virus vaccination or natural infection exhibit similar characteristics. J. Infect. Dis [print-electronic]. 2013 Jun 6/15/2013; 207(12): 1898-908. PMID: 23526830, PMCID: PMC3654755, PII: jit119, DOI: 10.1093/infdis/jit119, ISSN: 1537-6613.

Crowe JE. Immunology. Crowdsourcing immunity. Science. 2013 May 5/10/2013; 340(6133): 692-3. PMID: 23661747, PII: 340/6133/692, DOI: 10.1126/science.1238628, ISSN: 1095-9203.

Gilchuk P, Spencer CT, Conant SB, Hill T, Gray JJ, Niu X, Zheng M, Erickson JJ, Boyd KL, McAfee KJ, Oseroff C, Hadrup SR, Bennink JR, Hildebrand W, Edwards KM, Crowe JE, Williams JV, Buus S, Sette A, Schumacher TN, Link AJ, Joyce S. Discovering naturally processed antigenic determinants that confer protective T cell immunity. J. Clin. Invest [print-electronic]. 2013 May; 123(5): 1976-87. PMID: 23543059, PMCID: PMC3635741, PII: 67388, DOI: 10.1172/JCI67388, ISSN: 1558-8238.

Willis JR, Briney BS, DeLuca SL, Crowe JE, Meiler J. Human germline antibody gene segments encode polyspecific antibodies. PLoS Comput. Biol [print-electronic]. 2013 Apr; 9(4): e1003045. PMID: 23637590, PMCID: PMC3636087, PII: PCOMPBIOL-D-12-01934, DOI: 10.1371/journal.pcbi.1003045, ISSN: 1553-7358.

Bates JT, Keefer CJ, Utley TJ, Correia BE, Schief WR, Crowe JE. Reversion of somatic mutations of the respiratory syncytial virus-specific human monoclonal antibody Fab19 reveal a direct relationship between association rate and neutralizing potency. J. Immunol [print-electronic]. 2013 Apr 4/1/2013; 190(7): 3732-9. PMID: 23455501, PMCID: PMC3608519, PII: jimmunol.1202964, DOI: 10.4049/jimmunol.1202964, ISSN: 1550-6606.

Xu R, Krause JC, McBride R, Paulson JC, Crowe JE, Wilson IA. A recurring motif for antibody recognition of the receptor-binding site of influenza hemagglutinin. Nat. Struct. Mol. Biol [print-electronic]. 2013 Mar; 20(3): 363-70. PMID: 23396351, PMCID: PMC3594569, PII: nsmb.2500, DOI: 10.1038/nsmb.2500, ISSN: 1545-9985.

Hartman ML, Xu R, Crowe BJ, Robison LL, Erfurth EM, Kleinberg DL, Zimmermann AG, Woodmansee WW, Cutler GB, Chipman JJ, Melmed S, . Prospective safety surveillance of GH-deficient adults: comparison of GH-treated vs untreated patients. J. Clin. Endocrinol. Metab [print-electronic]. 2013 Mar; 98(3): 980-8. PMID: 23345098, PMCID: PMC3677286, PII: jc.2012-2684, DOI: 10.1210/jc.2012-2684, ISSN: 1945-7197.

Smith SA, de Alwis AR, Kose N, Harris E, Ibarra KD, Kahle KM, Pfaff JM, Xiang X, Doranz BJ, de Silva AM, Austin SK, Sukupolvi-Petty S, Diamond MS, Crowe JE. The potent and broadly neutralizing human dengue virus-specific monoclonal antibody 1C19 reveals a unique cross-reactive epitope on the bc loop of domain II of the envelope protein. MBio. 2013; 4(6): e00873-13. PMID: 24255124, PMCID: PMC3870244, PII: mBio.00873-13, DOI: 10.1128/mBio.00873-13, ISSN: 2150-7511.

Aiyegbo MS, Sapparapu G, Spiller BW, Eli IM, Williams DR, Kim R, Lee DE, Liu T, Li S, Woods VL, Nannemann DP, Meiler J, Stewart PL, Crowe JE. Human rotavirus VP6-specific antibodies mediate intracellular neutralization by binding to a quaternary structure in the transcriptional pore. PLoS ONE [electronic-print]. 2013; 8(5): e61101. PMID: 23671563, PMCID: PMC3650007, PII: PONE-D-13-06430, DOI: 10.1371/journal.pone.0061101, ISSN: 1932-6203.

Shaikh FY, Crowe JE. Molecular mechanisms driving respiratory syncytial virus assembly. Future Microbiol. 2013 Jan; 8(1): 123-31. PMID: 23252497, PMCID: PMC3577052, DOI: 10.2217/fmb.12.132, ISSN: 1746-0921.

Engelhardt BG, Sengsayadeth SM, Jagasia M, Savani BN, Kassim AA, Lu P, Shyr Y, Yoder SM, Rock MT, Crowe JE. Tissue-specific regulatory T cells: biomarker for acute graft-vs-host disease and survival. Exp. Hematol [print-electronic]. 2012 Dec; 40(12): 974-982.e1. PMID: 22885125, PMCID: PMC3611587, PII: S0301-472X(12)00343-8, DOI: 10.1016/j.exphem.2012.08.002, ISSN: 1873-2399.

Briney BS, Willis JR, Crowe JE. Location and length distribution of somatic hypermutation-associated DNA insertions and deletions reveals regions of antibody structural plasticity. Genes Immun [print-electronic]. 2012 Oct; 13(7): 523-9. PMID: 22717702, PMCID: PMC3449029, PII: gene201228, DOI: 10.1038/gene.2012.28, ISSN: 1476-5470.

Briney BS, Willis JR, McKinney BA, Crowe JE. High-throughput antibody sequencing reveals genetic evidence of global regulation of the naïve and memory repertoires that extends across individuals. Genes Immun [print-electronic]. 2012 Sep; 13(6): 469-73. PMID: 22622198, PII: gene201220, DOI: 10.1038/gene.2012.20, ISSN: 1476-5470.

Briney BS, Willis JR, Hicar MD, Thomas JW, Crowe JE. Frequency and genetic characterization of V(DD)J recombinants in the human peripheral blood antibody repertoire. Immunology. 2012 Sep; 137(1): 56-64. PMID: 22612413, PMCID: PMC3449247, DOI: 10.1111/j.1365-2567.2012.03605.x, ISSN: 1365-2567.

Krause JC, Tsibane T, Tumpey TM, Huffman CJ, Albrecht R, Blum DL, Ramos I, Fernandez-Sesma A, Edwards KM, García-Sastre A, Basler CF, Crowe JE. Human monoclonal antibodies to pandemic 1957 H2N2 and pandemic 1968 H3N2 influenza viruses. J. Virol [print-electronic]. 2012 Jun; 86(11): 6334-40. PMID: 22457520, PMCID: PMC3372199, PII: JVI.07158-11, DOI: 10.1128/JVI.07158-11, ISSN: 1098-5514.

Engelhardt BG, Jagasia SM, Crowe JE, Griffith ML, Savani BN, Kassim AA, Lu P, Weitkamp JH, Moore DJ, Yoder SM, Rock MT, Jagasia M. Predicting posttransplantation diabetes mellitus by regulatory T-cell phenotype: implications for metabolic intervention to modulate alloreactivity. Blood [print-electronic]. 2012 Mar 3/8/2012; 119(10): 2417-21. PMID: 22262764, PMCID: PMC3311262, PII: blood-2011-10-384750, DOI: 10.1182/blood-2011-10-384750, ISSN: 1528-0020.

Smith SA, Zhou Y, Olivarez NP, Broadwater AH, de Silva AM, Crowe JE. Persistence of circulating memory B cell clones with potential for dengue virus disease enhancement for decades following infection. J. Virol [print-electronic]. 2012 Mar; 86(5): 2665-75. PMID: 22171265, PMCID: PMC3302281, PII: JVI.06335-11, DOI: 10.1128/JVI.06335-11, ISSN: 1098-5514.

Crowe JE. Influenza virus resistance to human neutralizing antibodies. MBio [electronic-print]. 2012; 3(4): e00213-12. PMID: 22851660, PMCID: PMC3419524, PII: mBio.00213-12, DOI: 10.1128/mBio.00213-12, ISSN: 2150-7511.

Shaikh FY, Utley TJ, Craven RE, Rogers MC, Lapierre LA, Goldenring JR, Crowe JE. Respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins. PLoS ONE [print-electronic]. 2012; 7(7): e40826. PMID: 22808269, PMCID: PMC3396619, PII: PONE-D-12-11914, DOI: 10.1371/journal.pone.0040826, ISSN: 1932-6203.

Briney BS, Willis JR, Crowe JE. Human peripheral blood antibodies with long HCDR3s are established primarily at original recombination using a limited subset of germline genes. PLoS ONE [print-electronic]. 2012; 7(5): e36750. PMID: 22590602, PMCID: PMC3348910, PII: PONE-D-12-02931, DOI: 10.1371/journal.pone.0036750, ISSN: 1932-6203.

Shaikh FY, Cox RG, Lifland AW, Hotard AL, Williams JV, Moore ML, Santangelo PJ, Crowe JE. A critical phenylalanine residue in the respiratory syncytial virus fusion protein cytoplasmic tail mediates assembly of internal viral proteins into viral filaments and particles. MBio [electronic-print]. 2012; 3(1): PMID: 22318318, PMCID: PMC3280462, PII: mBio.00270-11, DOI: 10.1128/mBio.00270-11, ISSN: 2150-7511.

Thornburg NJ, Hayward SL, Crowe JE. Respiratory syncytial virus regulates human microRNAs by using mechanisms involving beta interferon and NF-¿B. MBio. 2012; 3(6): PMID: 23249809, PMCID: PMC3529541, PII: mBio.00220-12, DOI: 10.1128/mBio.00220-12, ISSN: 2150-7511.

Rock MT, McKinney BA, Yoder SM, Prudom CE, Wright DW, Crowe JE. Identification of potential human respiratory syncytial virus and metapneumovirus T cell epitopes using computational prediction and MHC binding assays. J. Immunol. Methods [print-electronic]. 2011 Nov 11/30/2011; 374(1-2): 13-7. PMID: 21854782, PMCID: PMC3220792, PII: S0022-1759(11)00209-2, DOI: 10.1016/j.jim.2011.08.004, ISSN: 1872-7905.

Krause JC, Tsibane T, Tumpey TM, Huffman CJ, Briney BS, Smith SA, Basler CF, Crowe JE. Epitope-specific human influenza antibody repertoires diversify by B cell intraclonal sequence divergence and interclonal convergence. J. Immunol [print-electronic]. 2011 Oct 10/1/2011; 187(7): 3704-11. PMID: 21880983, PMCID: PMC3178754, PII: jimmunol.1101823, DOI: 10.4049/jimmunol.1101823, ISSN: 1550-6606.

Krause JC, Tsibane T, Tumpey TM, Huffman CJ, Basler CF, Crowe JE. A broadly neutralizing human monoclonal antibody that recognizes a conserved, novel epitope on the globular head of the influenza H1N1 virus hemagglutinin. J. Virol [print-electronic]. 2011 Oct; 85(20): 10905-8. PMID: 21849447, PMCID: PMC3187471, PII: JVI.00700-11, DOI: 10.1128/JVI.00700-11, ISSN: 1098-5514.

Joyner AS, Willis JR, Crowe JE, Aiken C. Maturation-induced cloaking of neutralization epitopes on HIV-1 particles. PLoS Pathog [print-electronic]. 2011 Sep; 7(9): e1002234. PMID: 21931551, PMCID: PMC3169560, PII: PPATHOGENS-D-11-00125, DOI: 10.1371/journal.ppat.1002234, ISSN: 1553-7374.

Lindquist ME, Mainou BA, Dermody TS, Crowe JE. Activation of protein kinase R is required for induction of stress granules by respiratory syncytial virus but dispensable for viral replication. Virology [print-electronic]. 2011 Apr 4/25/2011; 413(1): 103-10. PMID: 21377708, PMCID: PMC3072468, PII: S0042-6822(11)00070-5, DOI: 10.1016/j.virol.2011.02.009, ISSN: 1096-0341.

Engelhardt BG, Jagasia M, Savani BN, Bratcher NL, Greer JP, Jiang A, Kassim AA, Lu P, Schuening F, Yoder SM, Rock MT, Crowe JE. Regulatory T cell expression of CLA or a(4)ß(7) and skin or gut acute GVHD outcomes. Bone Marrow Transplant [print-electronic]. 2011 Mar; 46(3): 436-42. PMID: 20577222, PMCID: PMC3217583, PII: bmt2010127, DOI: 10.1038/bmt.2010.127, ISSN: 1476-5365.

Krause JC, Ekiert DC, Tumpey TM, Smith PB, Wilson IA, Crowe JE. An insertion mutation that distorts antibody binding site architecture enhances function of a human antibody. MBio [electronic-print]. 2011; 2(1): e00345-10. PMID: 21304166, PMCID: PMC3037006, PII: mBio.00345-10, DOI: 10.1128/mBio.00345-10, ISSN: 2150-7511.

Pishchany G, McCoy AL, Torres VJ, Krause JC, Crowe JE, Fabry ME, Skaar EP. Specificity for human hemoglobin enhances Staphylococcus aureus infection. Cell Host Microbe. 2010 Dec 12/16/2010; 8(6): 544-50. PMID: 21147468, PMCID: PMC3032424, PII: S1931-3128(10)00355-0, DOI: 10.1016/j.chom.2010.11.002, ISSN: 1934-6069.

Thornburg NJ, Shepherd B, Crowe JE. Transforming growth factor beta is a major regulator of human neonatal immune responses following respiratory syncytial virus infection. J. Virol [print-electronic]. 2010 Dec; 84(24): 12895-902. PMID: 20926560, PMCID: PMC3004333, PII: JVI.01273-10, DOI: 10.1128/JVI.01273-10, ISSN: 1098-5514.

Lindquist ME, Lifland AW, Utley TJ, Santangelo PJ, Crowe JE. Respiratory syncytial virus induces host RNA stress granules to facilitate viral replication. J. Virol [print-electronic]. 2010 Dec; 84(23): 12274-84. PMID: 20844027, PMCID: PMC2976418, PII: JVI.00260-10, DOI: 10.1128/JVI.00260-10, ISSN: 1098-5514.

Hicar MD, Chen X, Briney B, Hammonds J, Wang JJ, Kalams S, Spearman PW, Crowe JE. Pseudovirion particles bearing native HIV envelope trimers facilitate a novel method for generating human neutralizing monoclonal antibodies against HIV. J. Acquir. Immune Defic. Syndr. 2010 Jul; 54(3): 223-35. PMID: 20531016, PMCID: PMC2930513, DOI: 10.1097/QAI.0b013e3181dc98a3, ISSN: 1944-7884.

Williams JV, Edwards KM, Weinberg GA, Griffin MR, Hall CB, Zhu Y, Szilagyi PG, Wang CK, Yang CF, Silva D, Ye D, Spaete RR, Crowe JE. Population-based incidence of human metapneumovirus infection among hospitalized children. J. Infect. Dis. 2010 Jun 6/15/2010; 201(12): 1890-8. PMID: 20446850, PMCID: PMC2873123, DOI: 10.1086/652782, ISSN: 1537-6613.

Hicar MD, Kalams SA, Spearman PW, Crowe JE. Emerging studies of human HIV-specific antibody repertoires. Vaccine. 2010 May 5/26/2010; 28 Suppl 2: B18-23. PMID: 20510738, PMCID: PMC2879399, PII: S0264-410X(10)00144-1, DOI: 10.1016/j.vaccine.2010.02.002, ISSN: 1873-2518.

Xu R, Ekiert DC, Krause JC, Hai R, Crowe JE, Wilson IA. Structural basis of preexisting immunity to the 2009 H1N1 pandemic influenza virus. Science [print-electronic]. 2010 Apr 4/16/2010; 328(5976): 357-60. PMID: 20339031, PMCID: PMC2897825, PII: science.1186430, DOI: 10.1126/science.1186430, ISSN: 1095-9203.

Krause JC, Tumpey TM, Huffman CJ, McGraw PA, Pearce MB, Tsibane T, Hai R, Basler CF, Crowe JE. Naturally occurring human monoclonal antibodies neutralize both 1918 and 2009 pandemic influenza A (H1N1) viruses. J. Virol [print-electronic]. 2010 Mar; 84(6): 3127-30. PMID: 20042511, PMCID: PMC2826039, PII: JVI.02184-09, DOI: 10.1128/JVI.02184-09, ISSN: 1098-5514.

Engelhardt BG, Crowe JE. Homing in on acute graft vs. host disease: tissue-specific T regulatory and Th17 cells. Curr. Top. Microbiol. Immunol. 2010; 341: 121-46. PMID: 20563712, PMCID: PMC3793903, DOI: 10.1007/82_2010_24, ISSN: 0070-217X.

Crowe JE. Recent advances in the study of human antibody responses to influenza virus using optimized human hybridoma approaches. Vaccine. 2009 Dec 12/30/2009; 27 Suppl 6: G47-51. PMID: 20006140, PMCID: PMC2835990, PII: S0264-410X(09)01675-2, DOI: 10.1016/j.vaccine.2009.10.124, ISSN: 1873-2518.

Talbot HK, Shepherd BE, Crowe JE, Griffin MR, Edwards KM, Podsiad AB, Tollefson SJ, Wright PF, Williams JV. The pediatric burden of human coronaviruses evaluated for twenty years. Pediatr. Infect. Dis. J. 2009 Aug; 28(8): 682-7. PMID: 19633513, PMCID: PMC2765860, PII: 00006454-200908000-00005, DOI: 10.1097/INF.0b013e31819d0d27, ISSN: 0891-3668.

Moore ML, Chi MH, Luongo C, Lukacs NW, Polosukhin VV, Huckabee MM, Newcomb DC, Buchholz UJ, Crowe JE, Goleniewska K, Williams JV, Collins PL, Peebles RS. A chimeric A2 strain of respiratory syncytial virus (RSV) with the fusion protein of RSV strain line 19 exhibits enhanced viral load, mucus, and airway dysfunction. J. Virol [print-electronic]. 2009 May; 83(9): 4185-94. PMID: 19211758, PMCID: PMC2668460, PII: JVI.01853-08, DOI: 10.1128/JVI.01853-08, ISSN: 1098-5514.

Talbot HK, Crowe JE, Edwards KM, Griffin MR, Zhu Y, Weinberg GA, Szilagyi PG, Hall CB, Podsiad AB, Iwane M, Williams JV, . Coronavirus infection and hospitalizations for acute respiratory illness in young children. J. Med. Virol. 2009 May; 81(5): 853-6. PMID: 19319948, PMCID: PMC2767383, DOI: 10.1002/jmv.21443, ISSN: 1096-9071.

Yang CF, Wang CK, Tollefson SJ, Piyaratna R, Lintao LD, Chu M, Liem A, Mark M, Spaete RR, Crowe JE, Williams JV. Genetic diversity and evolution of human metapneumovirus fusion protein over twenty years. Virol. J. 2009; 6: 138. PMID: 19740442, PMCID: PMC2753315, PII: 1743-422X-6-138, DOI: 10.1186/1743-422X-6-138, ISSN: 1743-422X.

Derdowski A, Peters TR, Glover N, Qian R, Utley TJ, Burnett A, Williams JV, Spearman P, Crowe JE. Human metapneumovirus nucleoprotein and phosphoprotein interact and provide the minimal requirements for inclusion body formation. J. Gen. Virol. 2008 Nov; 89(Pt 11): 2698-708. PMID: 18931065, PMCID: PMC2876975, PII: 89/11/2698, DOI: 10.1099/vir.0.2008/004051-0, ISSN: 0022-1317.

Mok H, Tollefson SJ, Podsiad AB, Shepherd BE, Polosukhin VV, Johnston RE, Williams JV, Crowe JE. An alphavirus replicon-based human metapneumovirus vaccine is immunogenic and protective in mice and cotton rats. J. Virol [print-electronic]. 2008 Nov; 82(22): 11410-8. PMID: 18786987, PMCID: PMC2573258, PII: JVI.01688-08, DOI: 10.1128/JVI.01688-08, ISSN: 1098-5514.

Yu X, Tsibane T, McGraw PA, House FS, Keefer CJ, Hicar MD, Tumpey TM, Pappas C, Perrone LA, Martinez O, Stevens J, Wilson IA, Aguilar PV, Altschuler EL, Basler CF, Crowe JE. Neutralizing antibodies derived from the B cells of 1918 influenza pandemic survivors. Nature [print-electronic]. 2008 Sep 9/25/2008; 455(7212): 532-6. PMID: 18716625, PMCID: PMC2848880, PII: nature07231, DOI: 10.1038/nature07231, ISSN: 1476-4687.

Mok H, Lee S, Wright DW, Crowe JE. Enhancement of the CD8+ T cell response to a subdominant epitope of respiratory syncytial virus by deletion of an immunodominant epitope. Vaccine [print-electronic]. 2008 Sep 9/2/2008; 26(37): 4775-82. PMID: 18662734, PMCID: PMC2561208, PII: S0264-410X(08)00934-1, DOI: 10.1016/j.vaccine.2008.07.012, ISSN: 0264-410X.

Yu X, McGraw PA, House FS, Crowe JE. An optimized electrofusion-based protocol for generating virus-specific human monoclonal antibodies. J. Immunol. Methods [print-electronic]. 2008 Jul 7/31/2008; 336(2): 142-51. PMID: 18514220, PMCID: PMC2519117, PII: S0022-1759(08)00139-7, DOI: 10.1016/j.jim.2008.04.008, ISSN: 0022-1759.

Utley TJ, Ducharme NA, Varthakavi V, Shepherd BE, Santangelo PJ, Lindquist ME, Goldenring JR, Crowe JE. Respiratory syncytial virus uses a Vps4-independent budding mechanism controlled by Rab11-FIP2. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2008 Jul 7/22/2008; 105(29): 10209-14. PMID: 18621683, PMCID: PMC2481327, PII: 0712144105, DOI: 10.1073/pnas.0712144105, ISSN: 1091-6490.

Kallewaard NL, McKinney BA, Gu Y, Chen A, Prasad BV, Crowe JE. Functional maturation of the human antibody response to rotavirus. J. Immunol. 2008 Mar 3/15/2008; 180(6): 3980-9. PMID: 18322207, PII: 180/6/3980, ISSN: 0022-1767.

Tian C, Luskin GK, Dischert KM, Higginbotham JN, Shepherd BE, Crowe JE. Immunodominance of the VH1-46 antibody gene segment in the primary repertoire of human rotavirus-specific B cells is reduced in the memory compartment through somatic mutation of nondominant clones. J. Immunol. 2008 Mar 3/1/2008; 180(5): 3279-88. PMID: 18292552, PII: 180/5/3279, ISSN: 0022-1767.

Mok H, Lee S, Utley TJ, Shepherd BE, Polosukhin VV, Collier ML, Davis NL, Johnston RE, Crowe JE. Venezuelan equine encephalitis virus replicon particles encoding respiratory syncytial virus surface glycoproteins induce protective mucosal responses in mice and cotton rats. J. Virol [print-electronic]. 2007 Dec; 81(24): 13710-22. PMID: 17928349, PMCID: PMC2168850, PII: JVI.01351-07, DOI: 10.1128/JVI.01351-07, ISSN: 1098-5514.

Williams JV, Chen Z, Cseke G, Wright DW, Keefer CJ, Tollefson SJ, Hessell A, Podsiad A, Shepherd BE, Sanna PP, Burton DR, Crowe JE, Williamson RA. A recombinant human monoclonal antibody to human metapneumovirus fusion protein that neutralizes virus in vitro and is effective therapeutically in vivo. J. Virol [print-electronic]. 2007 Aug; 81(15): 8315-24. PMID: 17522220, PMCID: PMC1951312, PII: JVI.00106-07, DOI: 10.1128/JVI.00106-07, ISSN: 0022-538X.

Lee S, Miller SA, Wright DW, Rock MT, Crowe JE. Tissue-specific regulation of CD8+ T-lymphocyte immunodominance in respiratory syncytial virus infection. J. Virol [print-electronic]. 2007 Mar; 81(5): 2349-58. PMID: 17182672, PMCID: PMC1865932, PII: JVI.01910-06, DOI: 10.1128/JVI.01910-06, ISSN: 0022-538X.

Tian C, Luskin GK, Dischert KM, Higginbotham JN, Shepherd BE, Crowe JE. Evidence for preferential Ig gene usage and differential TdT and exonuclease activities in human naïve and memory B cells. Mol. Immunol [print-electronic]. 2007 Mar; 44(9): 2173-83. PMID: 17196657, PMCID: PMC1859862, PII: S0161-5890(06)00701-2, DOI: 10.1016/j.molimm.2006.11.020, ISSN: 0161-5890.

Cseke G, Wright DW, Tollefson SJ, Johnson JE, Crowe JE, Williams JV. Human metapneumovirus fusion protein vaccines that are immunogenic and protective in cotton rats. J. Virol [print-electronic]. 2007 Jan; 81(2): 698-707. PMID: 17050599, PMCID: PMC1797435, PII: JVI.00844-06, DOI: 10.1128/JVI.00844-06, ISSN: 0022-538X.

Miller SA, Tollefson S, Crowe JE, Williams JV, Wright DW. Examination of a fusogenic hexameric core from human metapneumovirus and identification of a potent synthetic peptide inhibitor from the heptad repeat 1 region. J. Virol [print-electronic]. 2007 Jan; 81(1): 141-9. PMID: 17035305, PMCID: PMC1797239, PII: JVI.01243-06, DOI: 10.1128/JVI.01243-06, ISSN: 0022-538X.

Tian C, Kron GK, Dischert KM, Higginbotham JN, Crowe JE. Low expression of the interleukin (IL)-4 receptor alpha chain and reduced signalling via the IL-4 receptor complex in human neonatal B cells. Immunology [print-electronic]. 2006 Sep; 119(1): 54-62. PMID: 16764687, PMCID: PMC1782340, PII: IMM2405, DOI: 10.1111/j.1365-2567.2006.02405.x, ISSN: 0019-2805.

McKinney BA, Reif DM, Rock MT, Edwards KM, Kingsmore SF, Moore JH, Crowe JE. Cytokine expression patterns associated with systemic adverse events following smallpox immunization. J. Infect. Dis [print-electronic]. 2006 Aug 8/15/2006; 194(4): 444-53. PMID: 16845627, PMCID: PMC1620015, PII: JID36092, DOI: 10.1086/505503, ISSN: 0022-1899.

Rock MT, Yoder SM, Talbot TR, Edwards KM, Crowe JE. Cellular immune responses to diluted and undiluted aventis pasteur smallpox vaccine. J. Infect. Dis [print-electronic]. 2006 Aug 8/15/2006; 194(4): 435-43. PMID: 16845626, PII: JID36240, DOI: 10.1086/505506, ISSN: 0022-1899.

Williams JV, Wang CK, Yang CF, Tollefson SJ, House FS, Heck JM, Chu M, Brown JB, Lintao LD, Quinto JD, Chu D, Spaete RR, Edwards KM, Wright PF, Crowe JE. The role of human metapneumovirus in upper respiratory tract infections in children: a 20-year experience. J. Infect. Dis [print-electronic]. 2006 Feb 2/1/2006; 193(3): 387-95. PMID: 16388486, PMCID: PMC1586246, PII: JID34908, DOI: 10.1086/499274, ISSN: 0022-1899.

McKinney BA, Crowe JE, Voss HU, Crooke PS, Barney N, Moore JH. Hybrid grammar-based approach to nonlinear dynamical system identification from biological time series. Phys Rev E Stat Nonlin Soft Matter Phys [print-electronic]. 2006 Feb; 73(2 Pt 1): 21912. PMID: 16605367, DOI: 10.1103/PhysRevE.73.021912, ISSN: 1539-3755.

Bowen AL, Tian C, LaFleur BJ, Crowe JE. Transcriptional control of activation-induced cytidine deaminase and error-prone DNA polymerases is functionally mature in the B cells of infants at birth. Hum. Immunol [print-electronic]. 2006 Jan; 67(1-2): 43-6. PMID: 16698424, PII: S0198-8859(06)00008-5, DOI: 10.1016/j.humimm.2006.02.021, ISSN: 0198-8859.

Weitkamp JH, Lafleur BJ, Crowe JE. Rotavirus-specific CD5+ B cells in young children exhibit a distinct antibody repertoire compared with CD5- B cells. Hum. Immunol [print-electronic]. 2006 Jan; 67(1-2): 33-42. PMID: 16698423, PII: S0198-8859(06)00011-5, DOI: 10.1016/j.humimm.2006.02.024, ISSN: 0198-8859.

Brock SC, Heck JM, McGraw PA, Crowe JE. The transmembrane domain of the respiratory syncytial virus F protein is an orientation-independent apical plasma membrane sorting sequence. J. Virol. 2005 Oct; 79(19): 12528-35. PMID: 16160180, PMCID: PMC1211512, PII: 79/19/12528, DOI: 10.1128/JVI.79.19.12528-12535.2005, ISSN: 0022-538X.

Williams JV, Crowe JE, Enriquez R, Minton P, Peebles RS, Hamilton RG, Higgins S, Griffin M, Hartert TV. Human metapneumovirus infection plays an etiologic role in acute asthma exacerbations requiring hospitalization in adults. J. Infect. Dis [print-electronic]. 2005 Oct 10/1/2005; 192(7): 1149-53. PMID: 16136455, PMCID: PMC1476781, PII: JID34247, DOI: 10.1086/444392, ISSN: 0022-1899.

Williams JV, Martino R, Rabella N, Otegui M, Parody R, Heck JM, Crowe JE. A prospective study comparing human metapneumovirus with other respiratory viruses in adults with hematologic malignancies and respiratory tract infections. J. Infect. Dis [print-electronic]. 2005 Sep 9/15/2005; 192(6): 1061-5. PMID: 16107960, PMCID: PMC1483060, PII: JID34317, DOI: 10.1086/432732, ISSN: 0022-1899.

Williams JV, Tollefson SJ, Johnson JE, Crowe JE. The cotton rat (Sigmodon hispidus) is a permissive small animal model of human metapneumovirus infection, pathogenesis, and protective immunity. J. Virol. 2005 Sep; 79(17): 10944-51. PMID: 16103146, PMCID: PMC1193579, PII: 79/17/10944, DOI: 10.1128/JVI.79.17.10944-10951.2005, ISSN: 0022-538X.

Weitkamp JH, Lafleur BJ, Greenberg HB, Crowe JE. Natural evolution of a human virus-specific antibody gene repertoire by somatic hypermutation requires both hotspot-directed and randomly-directed processes. Hum. Immunol. 2005 Jun; 66(6): 666-76. PMID: 15993712, PII: S0198-8859(05)00060-1, DOI: 10.1016/j.humimm.2005.02.008, ISSN: 0198-8859.

Chen X, Rock MT, Hammonds J, Tartaglia J, Shintani A, Currier J, Slike B, Crowe JE, Marovich M, Spearman P. Pseudovirion particle production by live poxvirus human immunodeficiency virus vaccine vector enhances humoral and cellular immune responses. J. Virol. 2005 May; 79(9): 5537-47. PMID: 15827168, PMCID: PMC1082749, PII: 79/9/5537, DOI: 10.1128/JVI.79.9.5537-5547.2005, ISSN: 0022-538X.

Tai JH, Williams JV, Edwards KM, Wright PF, Crowe JE, Dermody TS. Prevalence of reovirus-specific antibodies in young children in Nashville, Tennessee. J. Infect. Dis [print-electronic]. 2005 Apr 4/15/2005; 191(8): 1221-4. PMID: 15776366, PII: JID33615, DOI: 10.1086/428911, ISSN: 0022-1899.

Bentzen EL, House F, Utley TJ, Crowe JE, Wright DW. Progression of respiratory syncytial virus infection monitored by fluorescent quantum dot probes. Nano Lett. 2005 Apr; 5(4): 591-5. PMID: 15826092, DOI: 10.1021/nl048073u, ISSN: 1530-6984.

Weitkamp JH, Kallewaard NL, Bowen AL, Lafleur BJ, Greenberg HB, Crowe JE. VH1-46 is the dominant immunoglobulin heavy chain gene segment in rotavirus-specific memory B cells expressing the intestinal homing receptor alpha4beta7. J. Immunol. 2005 Mar 3/15/2005; 174(6): 3454-60. PMID: 15749880, PII: 174/6/3454, ISSN: 0022-1767.

Rock MT, Yoder SM, Wright PF, Talbot TR, Edwards KM, Crowe JE. Differential regulation of granzyme and perforin in effector and memory T cells following smallpox immunization. J. Immunol. 2005 Mar 3/15/2005; 174(6): 3757-64. PMID: 15749916, PII: 174/6/3757, ISSN: 0022-1767.

Shaklee JF, Talbot TR, Muldowney JA, Vaughan DE, Butler J, House F, Crowe JE, Smith LH, Edwards KM. Smallpox vaccination does not elevate systemic levels of prothrombotic proteins associated with ischemic cardiac events. J. Infect. Dis [print-electronic]. 2005 Mar 3/1/2005; 191(5): 724-30. PMID: 15688286, PII: JID33581, DOI: 10.1086/427831, ISSN: 0022-1899.

Kallewaard NL, Bowen AL, Crowe JE. Cooperativity of actin and microtubule elements during replication of respiratory syncytial virus. Virology. 2005 Jan 1/5/2005; 331(1): 73-81. PMID: 15582654, PII: S0042-6822(04)00678-6, DOI: 10.1016/j.virol.2004.10.010, ISSN: 0042-6822.

Crowe JE. Human metapneumovirus as a major cause of human respiratory tract disease. Pediatr. Infect. Dis. J. 2004 Nov; 23(11 Suppl): S215-21. PMID: 15577576, PII: 00006454-200411001-00006, ISSN: 0891-3668.

Talbot TR, Stapleton JT, Brady RC, Winokur PL, Bernstein DI, Germanson T, Yoder SM, Rock MT, Crowe JE, Edwards KM. Vaccination success rate and reaction profile with diluted and undiluted smallpox vaccine: a randomized controlled trial. JAMA. 2004 Sep 9/8/2004; 292(10): 1205-12. PMID: 15353533, PII: 292/10/1205, DOI: 10.1001/jama.292.10.1205, ISSN: 1538-3598.

Rock MT, Yoder SM, Talbot TR, Edwards KM, Crowe JE. Adverse events after smallpox immunizations are associated with alterations in systemic cytokine levels. J. Infect. Dis [print-electronic]. 2004 Apr 4/15/2004; 189(8): 1401-10. PMID: 15073677, PII: JID31505, DOI: 10.1086/382510, ISSN: 0022-1899.

Suara RO, Crowe JE. Effect of zinc salts on respiratory syncytial virus replication. Antimicrob. Agents Chemother. 2004 Mar; 48(3): 783-90. PMID: 14982765, PMCID: PMC353050, ISSN: 0066-4804.

Williams JV, Harris PA, Tollefson SJ, Halburnt-Rush LL, Pingsterhaus JM, Edwards KM, Wright PF, Crowe JE. Human metapneumovirus and lower respiratory tract disease in otherwise healthy infants and children. N. Engl. J. Med. 2004 Jan 1/29/2004; 350(5): 443-50. PMID: 14749452, PMCID: PMC1831873, PII: 350/5/443, DOI: 10.1056/NEJMoa025472, ISSN: 1533-4406.

Brock SC, Goldenring JR, Crowe JE. Apical recycling systems regulate directional budding of respiratory syncytial virus from polarized epithelial cells. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2003 Dec 12/9/2003; 100(25): 15143-8. PMID: 14630951, PMCID: PMC299925, PII: 2434327100, DOI: 10.1073/pnas.2434327100, ISSN: 0027-8424.

Halasa NB, Whitlock JA, McCurley TL, Smith JA, Zhu Q, Ochs H, Dermody TS, Crowe JE. Fatal hemophagocytic lymphohistiocytosis associated with Epstein-Barr virus infection in a patient with a novel mutation in the signaling lymphocytic activation molecule-associated protein. Clin. Infect. Dis [print-electronic]. 2003 Nov 11/15/2003; 37(10): e136-41. PMID: 14583885, PII: CID31173, DOI: 10.1086/379126, ISSN: 1537-6591.

Weitkamp JH, Kallewaard N, Kusuhara K, Bures E, Williams JV, LaFleur B, Greenberg HB, Crowe JE. Infant and adult human B cell responses to rotavirus share common immunodominant variable gene repertoires. J. Immunol. 2003 Nov 11/1/2003; 171(9): 4680-8. PMID: 14568943, ISSN: 0022-1767.

Venter M, Rock M, Puren AJ, Tiemessen CT, Crowe JE. Respiratory syncytial virus nucleoprotein-specific cytotoxic T-cell epitopes in a South African population of diverse HLA types are conserved in circulating field strains. J. Virol. 2003 Jul; 77(13): 7319-29. PMID: 12805430, PMCID: PMC164818, ISSN: 0022-538X.

Crowe JE, Sannella EC, Pfeiffer S, Zorn GL, Azimzadeh A, Newman R, Miller GG, Pierson RN. CD154 regulates primate humoral immunity to influenza. Am. J. Transplant. 2003 Jun; 3(6): 680-8. PMID: 12780559, PII: 106, ISSN: 1600-6135.

Crowe JE, Williams JV. Immunology of viral respiratory tract infection in infancy. Paediatr Respir Rev. 2003 Jun; 4(2): 112-9. PMID: 12758048, PII: S1526054203000332, ISSN: 1526-0542.

Weitkamp JH, Kallewaard N, Kusuhara K, Feigelstock D, Feng N, Greenberg HB, Crowe JE. Generation of recombinant human monoclonal antibodies to rotavirus from single antigen-specific B cells selected with fluorescent virus-like particles. J. Immunol. Methods. 2003 Apr 4/1/2003; 275(1-2): 223-37. PMID: 12667686, PII: S0022175903000139, ISSN: 0022-1759.

Rock MT, Crowe JE. Identification of a novel human leucocyte antigen-A*01-restricted cytotoxic T-lymphocyte epitope in the respiratory syncytial virus fusion protein. Immunology. 2003 Apr; 108(4): 474-80. PMID: 12667209, PMCID: PMC1782928, PII: 1619, ISSN: 0019-2805.

Brock SC, McGraw PA, Wright PF, Crowe JE. The human polymeric immunoglobulin receptor facilitates invasion of epithelial cells by Streptococcus pneumoniae in a strain-specific and cell type-specific manner. Infect. Immun. 2002 Sep; 70(9): 5091-5. PMID: 12183558, PMCID: PMC128237, ISSN: 0019-9567.

Peters TR, Jennings MT, Crowe JE. Pneumocystis carinii pneumonia in a girl with a midbrain glioma. Pediatr Hematol Oncol. 2002 Mar; 19(2): 141-3. PMID: 11881789, ISSN: 0888-0018.

Crowe JE. Influence of maternal antibodies on neonatal immunization against respiratory viruses. Clin. Infect. Dis [print-electronic]. 2001 Nov 11/15/2001; 33(10): 1720-7. PMID: 11595986, PII: CID010273, DOI: 10.1086/322971, ISSN: 1058-4838.

Crowe JE. Respiratory syncytial virus vaccine development. Vaccine. 2001 Oct 10/15/2001; 20 Suppl 1: S32-7. PMID: 11587807, PII: S0264410X01002870, ISSN: 0264-410X.

Crowe JE, Firestone CY, Murphy BR. Passively acquired antibodies suppress humoral but not cell-mediated immunity in mice immunized with live attenuated respiratory syncytial virus vaccines. J. Immunol. 2001 Oct 10/1/2001; 167(7): 3910-8. PMID: 11564809, ISSN: 0022-1767.

Weitkamp JH, Crowe JE. Blood donor leukocyte reduction filters as a source of human B lymphocytes. BioTechniques. 2001 Sep; 31(3): 464-6. PMID: 11570486, ISSN: 0736-6205.

Pierson RN, Crowe JE, Pfeiffer S, Atkinson J, Azimzadeh A, Miller GG. CD40-ligand in primate cardiac allograft and viral immunity. Immunol. Res. 2001; 23(2-3): 253-62. PMID: 11444390, PII: IR:23:2-3:253, DOI: 10.1385/IR:23:2-3:253, ISSN: 0257-277X.

Crowe JE, Suara RO, Brock S, Kallewaard N, House F, Weitkamp JH. Genetic and structural determinants of virus neutralizing antibodies. Immunol. Res. 2001; 23(2-3): 135-45. PMID: 11444379, PII: IR:23:2-3:135, DOI: 10.1385/IR:23:2-3:135, ISSN: 0257-277X.

Peters TR, Brumbaugh DE, Lawton AR, Crowe JE. Recurrent pneumococcal arthritis as the presenting manifestation of X-linked agammaglobulinemia. Clin. Infect. Dis. 2000 Nov; 31(5): 1287-8. PMID: 11073766, PII: CID000256, DOI: 10.1086/317430, ISSN: 1058-4838.

Weitkamp JH, Tang YW, Haas DW, Midha NK, Crowe JE. Recurrent Achromobacter xylosoxidans bacteremia associated with persistent lymph node infection in a patient with hyper-immunoglobulin M syndrome. Clin. Infect. Dis [print-electronic]. 2000 Nov; 31(5): 1183-7. PMID: 11073750, PII: CID994327, DOI: 10.1086/317461, ISSN: 1058-4838.

Wright PF, Karron RA, Belshe RB, Thompson J, Crowe JE, Boyce TG, Halburnt LL, Reed GW, Whitehead SS, Anderson EL, Wittek AE, Casey R, Eichelberger M, Thumar B, Randolph VB, Udem SA, Chanock RM, Murphy BR. Evaluation of a live, cold-passaged, temperature-sensitive, respiratory syncytial virus vaccine candidate in infancy. J. Infect. Dis [print-electronic]. 2000 Nov; 182(5): 1331-42. PMID: 11010838, PII: JID000286, DOI: 10.1086/315859, ISSN: 0022-1899.

Pastey MK, Gower TL, Spearman PW, Crowe JE, Graham BS. A RhoA-derived peptide inhibits syncytium formation induced by respiratory syncytial virus and parainfluenza virus type 3. Nat. Med. 2000 Jan; 6(1): 35-40. PMID: 10613821, DOI: 10.1038/71503, ISSN: 1078-8956.

Weitkamp J-H, Crowe JE Jr. Current approaches to vaccine development against disease caused by respiratory syncytial virus (RSV). Monatsschr Kinderheilkd. 2000; 148: 980-9.

Fisher RG, Crowe JE, Johnson TR, Tang YW, Graham BS. Passive IgA monoclonal antibody is no more effective than IgG at protecting mice from mucosal challenge with respiratory syncytial virus. J. Infect. Dis. 1999 Oct; 180(4): 1324-7. PMID: 10479165, PII: JID990266, DOI: 10.1086/315037, ISSN: 0022-1899.

Pastey MK, Crowe JE, Graham BS. RhoA interacts with the fusion glycoprotein of respiratory syncytial virus and facilitates virus-induced syncytium formation. J. Virol. 1999 Sep; 73(9): 7262-70. PMID: 10438814, PMCID: PMC104251, ISSN: 0022-538X.

Sakurai H, Williamson RA, Crowe JE, Beeler JA, Poignard P, Bastidas RB, Chanock RM, Burton DR. Human antibody responses to mature and immature forms of viral envelope in respiratory syncytial virus infection: significance for subunit vaccines. J. Virol. 1999 Apr; 73(4): 2956-62. PMID: 10074145, PMCID: PMC104055, ISSN: 0022-538X.

Whitehead SS, Firestone CY, Karron RA, Crowe JE, Elkins WR, Collins PL, Murphy BR. Addition of a missense mutation present in the L gene of respiratory syncytial virus (RSV) cpts530/1030 to RSV vaccine candidate cpts248/404 increases its attenuation and temperature sensitivity. J. Virol. 1999 Feb; 73(2): 871-7. PMID: 9882287, PMCID: PMC103906, ISSN: 0022-538X.

Crowe JE, Randolph V, Murphy BR. The live attenuated subgroup B respiratory syncytial virus vaccine candidate RSV 2B33F is attenuated and immunogenic in chimpanzees, but exhibits partial loss of the ts phenotype following replication in vivo. Virus Res. 1999 Jan; 59(1): 13-22. PMID: 10854162, PII: S016817029800118X, ISSN: 0168-1702.

Crowe JE. Host responses to respiratory virus infection and immunization. Curr. Top. Microbiol. Immunol. 1999; 236: 191-214. PMID: 9893361, ISSN: 0070-217X.

Crowe JE Jr, Randolph V, Murphy BR. The live attenuated subgroup B respiratory syncytial virus vaccine candidate RSV 2B33F is attenuated and immunogenic in chimpanzees, but exhibits partial loss of the ts phenotype following replication in vivo. Virus Research. 1999; 59: 13-22.

Crowe JE, Firestone CY, Crim R, Beeler JA, Coelingh KL, Barbas CF, Burton DR, Chanock RM, Murphy BR. Monoclonal antibody-resistant mutants selected with a respiratory syncytial virus-neutralizing human antibody fab fragment (Fab 19) define a unique epitope on the fusion (F) glycoprotein. Virology. 1998 Dec 12/20/1998; 252(2): 373-5. PMID: 9878616, PII: S0042-6822(98)99462-4, DOI: 10.1006/viro.1998.9462, ISSN: 0042-6822.

Crowe JE. Immune responses of infants to infection with respiratory viruses and live attenuated respiratory virus candidate vaccines. Vaccine. 1998 Aug; 16(14-15): 1423-32. PMID: 9711783, PII: S0264-410X(98)00103-0, ISSN: 0264-410X.

Crowe JE, Gilmour PS, Murphy BR, Chanock RM, Duan L, Pomerantz RJ, Pilkington GR. Isolation of a second recombinant human respiratory syncytial virus monoclonal antibody fragment (Fab RSVF2-5) that exhibits therapeutic efficacy in vivo. J. Infect. Dis. 1998 Apr; 177(4): 1073-6. PMID: 9534985, ISSN: 0022-1899.

Karron RA, Wright PF, Crowe JE, Clements-Mann ML, Thompson J, Makhene M, Casey R, Murphy BR. Evaluation of two live, cold-passaged, temperature-sensitive respiratory syncytial virus vaccines in chimpanzees and in human adults, infants, and children. J. Infect. Dis. 1997 Dec; 176(6): 1428-36. PMID: 9395351, ISSN: 0022-1899.

Juhasz K, Whitehead SS, Bui PT, Biggs JM, Crowe JE, Boulanger CA, Collins PL, Murphy BR. The temperature-sensitive (ts) phenotype of a cold-passaged (cp) live attenuated respiratory syncytial virus vaccine candidate, designated cpts530, results from a single amino acid substitution in the L protein. J. Virol. 1997 Aug; 71(8): 5814-9. PMID: 9223470, PMCID: PMC191836, ISSN: 0022-538X.

Crowe JE Jr, Murphy BR. Vaccines against respiratory syncytial virus and parainfluenza type 3. New Generation Vaccines, 2nd ed, Levine MM, Woodrow GC, Kaper JB, Cobon GS. 1997; 711-25.

Firestone CY, Whitehead SS, Collins PL, Murphy BR, Crowe JE. Nucleotide sequence analysis of the respiratory syncytial virus subgroup A cold-passaged (cp) temperature sensitive (ts) cpts-248/404 live attenuated virus vaccine candidate. Virology. 1996 Nov 11/15/1996; 225(2): 419-22. PMID: 8918930, PII: S0042682296906182, ISSN: 0042-6822.

Crowe JE, Bui PT, Firestone CY, Connors M, Elkins WR, Chanock RM, Murphy BR. Live subgroup B respiratory syncytial virus vaccines that are attenuated, genetically stable, and immunogenic in rodents and nonhuman primates. J. Infect. Dis. 1996 Apr; 173(4): 829-39. PMID: 8603960, ISSN: 0022-1899.

Crowe JE, Firestone CY, Whitehead SS, Collins PL, Murphy BR. Acquisition of the ts phenotype by a chemically mutagenized cold-passaged human respiratory syncytial virus vaccine candidate results from the acquisition of a single mutation in the polymerase (L) gene. Virus Genes. 1996; 13(3): 269-73. PMID: 9035372, ISSN: 0920-8569.

Crowe JE Jr. The role of antibodies in respiratory viral immunity. Seminars in Virology. 1996; 7: 273-83.

Crowe JE, Bui PT, Siber GR, Elkins WR, Chanock RM, Murphy BR. Cold-passaged, temperature-sensitive mutants of human respiratory syncytial virus (RSV) are highly attenuated, immunogenic, and protective in seronegative chimpanzees, even when RSV antibodies are infused shortly before immunization. Vaccine. 1995 Jun; 13(9): 847-55. PMID: 7483808, PII: 0264410X9400074W, ISSN: 0264-410X.

Connors M, Crowe JE, Firestone CY, Murphy BR, Collins PL. A cold-passaged, attenuated strain of human respiratory syncytial virus contains mutations in the F and L genes. Virology. 1995 Apr 4/20/1995; 208(2): 478-84. PMID: 7747420, PII: S0042-6822(85)71178-6, DOI: 10.1006/viro.1995.1178, ISSN: 0042-6822.

Hsu KH, Crowe JE, Lubeck MD, Davis AR, Hung PP, Chanock RM, Murphy BR. Isolation and characterization of a highly attenuated respiratory syncytial virus (RSV) vaccine candidate by mutagenesis of the incompletely attenuated RSV A2 ts-1 NG-1 mutant virus. Vaccine. 1995 Apr; 13(5): 509-15. PMID: 7543716, PII: 0264410X94000025, ISSN: 0264-410X.

Crowe JE. Current approaches to the development of vaccines against disease caused by respiratory syncytial virus (RSV) and parainfluenza virus (PIV). A meeting report of the WHO Programme for Vaccine Development. Vaccine. 1995 Mar; 13(4): 415-21. PMID: 7793140, PII: 0264-410X(95)98266-D, ISSN: 0264-410X.

Kulkarni AB, Collins PL, Bacik I, Yewdell JW, Bennink JR, Crowe JE, Murphy BR. Cytotoxic T cells specific for a single peptide on the M2 protein of respiratory syncytial virus are the sole mediators of resistance induced by immunization with M2 encoded by a recombinant vaccinia virus. J. Virol. 1995 Feb; 69(2): 1261-4. PMID: 7815502, PMCID: PMC188700, ISSN: 0022-538X.

Crowe JE, Cheung PY, Wallace EF, Chanock RM, Larrick JW, Murphy BR, Fry K. Isolation and characterization of a chimpanzee monoclonal antibody to the G glycoprotein of human respiratory syncytial virus. Clin. Diagn. Lab. Immunol. 1994 Nov; 1(6): 701-6. PMID: 8556524, PMCID: PMC368396, ISSN: 1071-412X.

Crowe JE, Bui PT, Davis AR, Chanock RM, Murphy BR. A further attenuated derivative of a cold-passaged temperature-sensitive mutant of human respiratory syncytial virus retains immunogenicity and protective efficacy against wild-type challenge in seronegative chimpanzees. Vaccine. 1994 Jul; 12(9): 783-90. PMID: 7975856, ISSN: 0264-410X.

Crowe JE, Bui PT, London WT, Davis AR, Hung PP, Chanock RM, Murphy BR. Satisfactorily attenuated and protective mutants derived from a partially attenuated cold-passaged respiratory syncytial virus mutant by introduction of additional attenuating mutations during chemical mutagenesis. Vaccine. 1994 Jun; 12(8): 691-9. PMID: 8091846, ISSN: 0264-410X.

Murphy BR, Hall SL, Kulkarni AB, Crowe JE, Collins PL, Connors M, Karron RA, Chanock RM. An update on approaches to the development of respiratory syncytial virus (RSV) and parainfluenza virus type 3 (PIV3) vaccines. Virus Res. 1994 Apr; 32(1): 13-36. PMID: 8030364, PII: 0168-1702(94)90059-0, ISSN: 0168-1702.

Crowe JE, Murphy BR, Chanock RM, Williamson RA, Barbas CF, Burton DR. Recombinant human respiratory syncytial virus (RSV) monoclonal antibody Fab is effective therapeutically when introduced directly into the lungs of RSV-infected mice. Proc. Natl. Acad. Sci. U.S.A. 1994 Feb 2/15/1994; 91(4): 1386-90. PMID: 8108420, PMCID: PMC43163, ISSN: 0027-8424.

Crowe JE Jr, Murphy BR, Chanock RM, Williamson A, Barbas CF III, Burton DR. Human RSV monoclonal antibody Fab cloned from a combinatorial library and produced in E. coli is effective therapeutically when introduced directly into the lungs of RSV-infected mice. Brown F, Chanock RM, Ginsberg HS, Norrby E, eds. Vaccines 94: Modern Approaches to New Vaccines Including Prevention of AIDS. Cold Spring Harbor: Cold Spring Harbor Laboratory Press. 1994; 315-20.

Murphy BR, Crowe JE Jr, Lubeck MD, Hsu K-HL, Hall SL, Karron RA, Clements ML, Wright PF, Belshe RB, Chanock RM. Live attenuated vaccines for respiratory syncytial virus (RSV) and parainfluenza virus type 3 (PIV 3). Report of the 104th Ross Conference on Pediatric Research, published by Ross Products. 1994; 173-8.

Crowe JE, Collins PL, London WT, Chanock RM, Murphy BR. A comparison in chimpanzees of the immunogenicity and efficacy of live attenuated respiratory syncytial virus (RSV) temperature-sensitive mutant vaccines and vaccinia virus recombinants that express the surface glycoproteins of RSV. Vaccine. 1993 Nov; 11(14): 1395-404. PMID: 8310760, ISSN: 0264-410X.

Chanock RM, Crowe JE, Murphy BR, Burton DR. Human monoclonal antibody Fab fragments cloned from combinatorial libraries: potential usefulness in prevention and/or treatment of major human viral diseases. Infect Agents Dis. 1993 Jun; 2(3): 118-31. PMID: 8173784, ISSN: 1056-2044.

Crowe JE Jr, Barbas CF III, Bui P, Cababa D, Jones TM, Zebedee SL, Murphy BR, Chanock RM, Burton DR. Human monoclonal Fab fragments derived from a combinatorial library bind to respiratory syncytial virus F glycoprotein and neutralize infectivity. Brown F, Chanock RM, Ginsberg HS, Lerner RA, eds. Vaccines 93: Modern Approaches to New Vaccines Including Prevention of AIDS. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory. 1993; (()): 7-11.

Barbas CF, Crowe JE, Cababa D, Jones TM, Zebedee SL, Murphy BR, Chanock RM, Burton DR. Human monoclonal Fab fragments derived from a combinatorial library bind to respiratory syncytial virus F glycoprotein and neutralize infectivity. Proc. Natl. Acad. Sci. U.S.A. 1992 Nov 11/1/1992; 89(21): 10164-8. PMID: 1279672, PMCID: PMC50298, ISSN: 0027-8424.

Murphy BR, Hall SL, Crowe JE Jr, Collins PL, Subbarao EK, Connors M, London WT, Chanock RM. The use of chimpanzees in respiratory virus research. Erwin J, Landon JC, eds. Chimpanzee Conservation and Public Health: Environments for the Future. 1992; 21 28.