Albert B. Reynolds, Ph.D.

Professor

al.reynolds@vanderbilt.edu
Faculty Appointments
Professor of Cancer Biology Ingram Professor of Cancer Research
Education
Ph.D., Cell Biology, Anatomy, University of Virginia, Charlottesville, VirginiaB.A., Biology, Kenyon College, Gambier, Ohio
Office Address
771 PRB
2220 Pierce Ave.
Nashville, TN 37232-6840
Research Description
My laboratory combines molecular and biochemical approaches with 3D culture and mouse models of colon and breast cancer to study the roles of p120-catenin (p120) in cell-cell adhesion, tumorigenesis and metastasis. Most proteins linked physically or functionally to p120 are, in fact, tumor suppressors or oncogenes (eg, Src, Receptor Tyrosine kinases, Rho GTPases, E-cadherin, B-catenin, Adenomatous Polyposis Coli), implying a role for p120 in cancer. Previously, we showed that p120 interaction is essential for cadherin stability at the cell surface. For example, p120 knockdown in many epithelial cell lines (eg, MCF10A, A431) causes rapid degradation of the entire E-cadherin complex and partial or complete loss of cell-cell adhesion. These observations have led to the notion that p120 itself is a tumor and/or metastasis suppressor - on its own, or in collaboration with E-cadherin. Indeed, E-cadherin is broadly established as a tumor and metastasis suppressor and p120 is frequently downregulated and/or mislocalized in most major human cancers (e.g. colon, breast, lung, pancreas, prostate).

We are heavily invested in mouse models of colon cancer. Using conditional p120 KO in APC mutant mice, we recently found that p120 is, in fact, a haploinsufficient tumor suppressor in the colon (and probably other tissues). Interestingly, loss of one p120 allele increases tumor number by 10 fold. Although biallic loss is well tolerated in wild type intestinal epithelium, it is not permitted in the context of the tumor. Based on data from Sleeping Beauty insertional mutagenesis screens (in collaboration with Neal Copeland) we have extended these findings to a-catenin and E-cadherin, and to several different oncogenic backgrounds (e.g., K-Ras, Smad4, p53). Thus, it appears that loss of E-cadherin complex components is not restricted to late stage tumors. Instead, very early loss of any single allele for p120, a-catenin or E-cadherin likely comprises a major bottleneck to tumor progression, exceeded only by the mandatory initiating mutation in APC.

In the mammary gland, we are using a well-characterized MMTV-mT breast cancer model to examine the role of p120 in metastasis. Interestingly, in contrast to the intestine, the mammary gland does not form at all in the absence of p120. Transformation by mT, on the other hand, rescues the epithelium and gives rise to p120 null primary tumors. However, tumor cells lacking p120 cannot metastasize to the lung. Thus, we have arrived at a unique and powerful system for examining not only the role of p120 (and p120 isoforms) in metastasis, but also recently postulated roles for EMT and ???stemness??? as they pertain to the metastatic process. The system is complemented by mechanistic studies based on ex-vivo manipulation of cells follow by reconstitution in vivo or in in vitro 3D cell cultures.

Finally, A third line of research focuses on the transcription factor Kaiso, discovered some time ago in our lab as a direct p120 binding partner. The interaction is reminiscent of Wnt-pathway mediated B-catenin ??? TCF4 interactions and may connect p120 to the nucleus. The Kaiso landscape is evolving rapidly in the wake of whole genome sequencing technologies such as ChipSEQ. We are examining different aspects of Kaiso activity in intestinal and mammary systems and moving into exciting new areas including epigenetics, transcription and genome maintenance. Kaiso is going to be an exciting place to be over the next decade.
Research Keywords
p120-catenin and Kaiso function in cell-cell adhesion and cancer
Publications
Chignalia AZ, Vogel SM, Reynolds AB, Mehta D, Dull RO, Minshall RD, Malik AB, Liu Y. P120-catenin expressed in alveolar type II cells is essential for the regulation of lung innate immune response. Am. J. Pathol [print-electronic]. 2015 May; 185(5): 1251-63. PMID: 25773174, PMCID: PMC4419206, PII: S0002-9440(15)00091-7, DOI: 10.1016/j.ajpath.2015.01.022, ISSN: 1525-2191.

Hendley AM, Provost E, Bailey JM, Wang YJ, Cleveland MH, Blake D, Bittman RW, Roeser JC, Maitra A, Reynolds AB, Leach SD. P120 Catenin is required for normal tubulogenesis but not epithelial integrity in developing mouse pancreas. Dev. Biol [print-electronic]. 2015 Mar 3/1/2015; 399(1): 41-53. PMID: 25523391, PII: S0012-1606(14)00636-8, DOI: 10.1016/j.ydbio.2014.12.010, ISSN: 1095-564X.

Huapeng Yu, Michael Dohn, Nicolas Markham, Robert Coffey, and Albert B. Reynolds.. P120-catenin controls contractility along the vertical axis of epithelial lateral membranes. 2015.

Koh DI, Han D, Ryu H, Choi WI, Jeon BN, Kim MK, Kim Y, Kim JY, Parry L, Clarke AR, Reynolds AB, Hur MW. KAISO, a critical regulator of p53-mediated transcription of CDKN1A and apoptotic genes. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2014 Oct 10/21/2014; 111(42): 15078-83. PMID: 25288747, PMCID: PMC4210320, PII: 1318780111, DOI: 10.1073/pnas.1318780111, ISSN: 1091-6490.

Reynolds AB, Kanner SB, Bouton AH, Schaller MD, Weed SA, Flynn DC, Parsons JT. SRChing for the substrates of Src. Oncogene [print-electronic]. 2014 Sep 9/11/2014; 33(37): 4537-47. PMID: 24121272, PII: onc2013416, DOI: 10.1038/onc.2013.416, ISSN: 1476-5594.

Markham NO, Doll CA, Dohn MR, Miller RK, Yu H, Coffey RJ, McCrea PD, Gamse JT, Reynolds AB. DIPA-family coiled-coils bind conserved isoform-specific head domain of p120-catenin family: potential roles in hydrocephalus and heterotopia. Mol. Biol. Cell [print-electronic]. 2014 Sep 9/1/2014; 25(17): 2592-603. PMID: 25009281, PMCID: PMC4148249, PII: mbc.E13-08-0492, DOI: 10.1091/mbc.E13-08-0492, ISSN: 1939-4586.

Yang Z, Sun D, Yan Z, Reynolds AB, Christman JW, Minshall RD, Malik AB, Zhang Y, Hu G. Differential role for p120-catenin in regulation of TLR4 signaling in macrophages. J. Immunol [print-electronic]. 2014 Aug 8/15/2014; 193(4): 1931-41. PMID: 25015829, PMCID: PMC4119481, PII: jimmunol.1302863, DOI: 10.4049/jimmunol.1302863, ISSN: 1550-6606.

Lang RA, Herman K, Reynolds AB, Hildebrand JD, Plageman TF. P120-catenin-dependent junctional recruitment of Shroom3 is required for apical constriction during lens pit morphogenesis. Development [print-electronic]. 2014 Aug; 141(16): 3177-87. PMID: 25038041, PMCID: PMC4197547, PII: dev.107433, DOI: 10.1242/dev.107433, ISSN: 1477-9129.

Bryce NS, Reynolds AB, Koleske AJ, Weaver AM. WAVE2 regulates epithelial morphology and cadherin isoform switching through regulation of Twist and Abl. PLoS ONE [electronic-print]. 2013; 8(5): e64533. PMID: 23691243, PMCID: PMC3654908, PII: PONE-D-11-07007, DOI: 10.1371/journal.pone.0064533, ISSN: 1932-6203.

Markham NO, Cooper T, Goff M, Gribben EM, Carnahan RH, Reynolds AB. Monoclonal antibodies to DIPA: a novel binding partner of p120-catenin isoform 1. Hybridoma (Larchmt). 2012 Aug; 31(4): 246-54. PMID: 22894777, PMCID: PMC3420552, DOI: 10.1089/hyb.2012.0009, ISSN: 1557-8348.

Kurley SJ, Bierie B, Carnahan RH, Lobdell NA, Davis MA, Hofmann I, Moses HL, Muller WJ, Reynolds AB. P120-catenin is essential for terminal end bud function and mammary morphogenesis. Development [print-electronic]. 2012 May; 139(10): 1754-64. PMID: 22461563, PMCID: PMC3328177, PII: dev.072769, DOI: 10.1242/dev.072769, ISSN: 1477-9129.

Barrett CW, Smith JJ, Lu LC, Markham N, Stengel KR, Short SP, Zhang B, Hunt AA, Fingleton BM, Carnahan RH, Engel ME, Chen X, Beauchamp RD, Wilson KT, Hiebert SW, Reynolds AB, Williams CS. Kaiso directs the transcriptional corepressor MTG16 to the Kaiso binding site in target promoters. PLoS ONE [print-electronic]. 2012; 7(12): e51205. PMID: 23251453, PMCID: PMC3521008, PII: PONE-D-12-22965, DOI: 10.1371/journal.pone.0051205, ISSN: 1932-6203.

Smith AL, Dohn MR, Brown MV, Reynolds AB. Association of Rho-associated protein kinase 1 with E-cadherin complexes is mediated by p120-catenin. Mol. Biol. Cell [print-electronic]. 2012 Jan; 23(1): 99-110. PMID: 22031287, PMCID: PMC3248908, PII: mbc.E11-06-0497, DOI: 10.1091/mbc.E11-06-0497, ISSN: 1939-4586.

Reynolds AB. Epithelial organization: new perspective on a-catenin from an ancient source. Curr. Biol. 2011 Jun 6/7/2011; 21(11): R430-2. PMID: 21640901, PII: S0960-9822(11)00485-4, DOI: 10.1016/j.cub.2011.04.043, ISSN: 1879-0445.

Smalley-Freed WG, Efimov A, Short SP, Jia P, Zhao Z, Washington MK, Robine S, Coffey RJ, Reynolds AB. Adenoma formation following limited ablation of p120-catenin in the mouse intestine. PLoS ONE [print-electronic]. 2011; 6(5): e19880. PMID: 21611205, PMCID: PMC3096651, PII: PONE-D-11-01297, DOI: 10.1371/journal.pone.0019880, ISSN: 1932-6203.

Smith AL, Friedman DB, Yu H, Carnahan RH, Reynolds AB. ReCLIP (reversible cross-link immuno-precipitation): an efficient method for interrogation of labile protein complexes. PLoS ONE. 2011; 6(1): e16206. PMID: 21283770, PMCID: PMC3024417, DOI: 10.1371/journal.pone.0016206, ISSN: 1932-6203.

Donowitz M, Singh S, Singh P, Salahuddin FF, Chen Y, Chakraborty M, Murtazina R, Gucek M, Cole RN, Zachos NC, Kovbasnjuk O, Broere N, Smalley-Freed WG, Reynolds AB, Hubbard AL, Seidler U, Weinman E, de Jonge HR, Hogema BM, Li X. Alterations in the proteome of the NHERF1 knockout mouse jejunal brush border membrane vesicles. Physiol. Genomics [print-electronic]. 2010 Nov 11/15/2010; 42A(3): 200-10. PMID: 20736413, PMCID: PMC3008364, PII: physiolgenomics.00001.2010, DOI: 10.1152/physiolgenomics.00001.2010, ISSN: 1531-2267.

Wong LE, Reynolds AB, Dissanayaka NT, Minden A. P120-catenin is a binding partner and substrate for Group B Pak kinases. J. Cell. Biochem. 2010 Aug 8/1/2010; 110(5): 1244-54. PMID: 20564219, DOI: 10.1002/jcb.22639, ISSN: 1097-4644.

Smalley-Freed WG, Efimov A, Burnett PE, Short SP, Davis MA, Gumucio DL, Washington MK, Coffey RJ, Reynolds AB. P120-catenin is essential for maintenance of barrier function and intestinal homeostasis in mice. J. Clin. Invest [print-electronic]. 2010 Jun; 120(6): 1824-35. PMID: 20484816, PMCID: PMC2877948, PII: 41414, DOI: 10.1172/JCI41414, ISSN: 1558-8238.

Reynolds AB. Exposing p120 catenin's most intimate affair. Cell. 2010 Apr 4/2/2010; 141(1): 20-2. PMID: 20371340, PII: S0092-8674(10)00295-3, DOI: 10.1016/j.cell.2010.03.022, ISSN: 1097-4172.

Oas RG, Xiao K, Summers S, Wittich KB, Chiasson CM, Martin WD, Grossniklaus HE, Vincent PA, Reynolds AB, Kowalczyk AP. P120-Catenin is required for mouse vascular development. Circ. Res [print-electronic]. 2010 Mar 3/19/2010; 106(5): 941-51. PMID: 20110533, PMCID: PMC2859711, PII: CIRCRESAHA.109.207753, DOI: 10.1161/CIRCRESAHA.109.207753, ISSN: 1524-4571.

Carnahan RH, Rokas A, Gaucher EA, Reynolds AB. The molecular evolution of the p120-catenin subfamily and its functional associations. PLoS ONE. 2010; 5(12): e15747. PMID: 21209830, PMCID: PMC3013132, DOI: 10.1371/journal.pone.0015747, ISSN: 1932-6203.

Anderson AR, Hassanein M, Branch KM, Lu J, Lobdell NA, Maier J, Basanta D, Weidow B, Narasanna A, Arteaga CL, Reynolds AB, Quaranta V, Estrada L, Weaver AM. Microenvironmental independence associated with tumor progression. Cancer Res [print-electronic]. 2009 Nov 11/15/2009; 69(22): 8797-806. PMID: 19887618, PMCID: PMC2783510, PII: 0008-5472.CAN-09-0437, DOI: 10.1158/0008-5472.CAN-09-0437, ISSN: 1538-7445.

Dohn MR, Brown MV, Reynolds AB. An essential role for p120-catenin in Src- and Rac1-mediated anchorage-independent cell growth. J. Cell Biol [print-electronic]. 2009 Feb 2/9/2009; 184(3): 437-50. PMID: 19188496, PMCID: PMC2646551, PII: jcb.200807096, DOI: 10.1083/jcb.200807096, ISSN: 1540-8140.

Brown MV, Burnett PE, Denning MF, Reynolds AB. PDGF receptor activation induces p120-catenin phosphorylation at serine 879 via a PKCalpha-dependent pathway. Exp. Cell Res [print-electronic]. 2009 Jan 1/1/2009; 315(1): 39-49. PMID: 18950621, PMCID: PMC2925109, PII: S0014-4827(08)00386-8, DOI: 10.1016/j.yexcr.2008.09.025, ISSN: 1090-2422.

Ogden SR, Wroblewski LE, Weydig C, Romero-Gallo J, O'Brien DP, Israel DA, Krishna US, Fingleton B, Reynolds AB, Wessler S, Peek RM. P120 and Kaiso regulate Helicobacter pylori-induced expression of matrix metalloproteinase-7. Mol. Biol. Cell [print-electronic]. 2008 Oct; 19(10): 4110-21. PMID: 18653469, PMCID: PMC2555941, PII: E08-03-0283, DOI: 10.1091/mbc.E08-03-0283, ISSN: 1939-4586.

Fukumoto Y, Shintani Y, Reynolds AB, Johnson KR, Wheelock MJ. The regulatory or phosphorylation domain of p120 catenin controls E-cadherin dynamics at the plasma membrane. Exp. Cell Res [print-electronic]. 2008 Jan 1/1/2008; 314(1): 52-67. PMID: 17719574, PMCID: PMC2211447, PII: S0014-4827(07)00355-2, DOI: 10.1016/j.yexcr.2007.07.024, ISSN: 0014-4827.

Vaughan MH, Xia X, Wang X, Chronopoulou E, Gao GJ, Campos-Gonzalez R, Reynolds AB. Generation and characterization of a novel phospho-specific monoclonal antibody to p120-catenin serine 879. Hybridoma (Larchmt). 2007 Dec; 26(6): 407-15. PMID: 18158786, DOI: 10.1089/hyb.2007.0527, ISSN: 1554-0014.

Ezaki T, Guo RJ, Li H, Reynolds AB, Lynch JP. The homeodomain transcription factors Cdx1 and Cdx2 induce E-cadherin adhesion activity by reducing beta- and p120-catenin tyrosine phosphorylation. Am. J. Physiol. Gastrointest. Liver Physiol [print-electronic]. 2007 Jul; 293(1): G54-65. PMID: 17463179, PII: 00533.2006, DOI: 10.1152/ajpgi.00533.2006, ISSN: 0193-1857.

Reynolds AB. P120-catenin: Past and present. Biochim. Biophys. Acta [print-electronic]. 2007 Jan; 1773(1): 2-7. PMID: 17175391, PMCID: PMC2892545, PII: S0167-4889(06)00295-3, DOI: 10.1016/j.bbamcr.2006.09.019, ISSN: 0006-3002.

Wildenberg GA, Dohn MR, Carnahan RH, Davis MA, Lobdell NA, Settleman J, Reynolds AB. P120-catenin and p190RhoGAP regulate cell-cell adhesion by coordinating antagonism between Rac and Rho. Cell. 2006 Dec 12/1/2006; 127(5): 1027-39. PMID: 17129786, PII: S0092-8674(06)01407-3, DOI: 10.1016/j.cell.2006.09.046, ISSN: 0092-8674.

Madhavan R, Zhao XT, Reynolds AB, Peng HB. Involvement of p120 catenin in myopodial assembly and nerve-muscle synapse formation. J. Neurobiol. 2006 Nov; 66(13): 1511-27. PMID: 17031840, DOI: 10.1002/neu.20320, ISSN: 0022-3034.

Xia X, Carnahan RH, Vaughan MH, Wildenberg GA, Reynolds AB. P120 serine and threonine phosphorylation is controlled by multiple ligand-receptor pathways but not cadherin ligation. Exp. Cell Res [print-electronic]. 2006 Oct 10/15/2006; 312(17): 3336-48. PMID: 16935280, PII: S0014-4827(06)00274-6, DOI: 10.1016/j.yexcr.2006.07.007, ISSN: 0014-4827.

Perez-Moreno M, Davis MA, Wong E, Pasolli HA, Reynolds AB, Fuchs E. P120-catenin mediates inflammatory responses in the skin. Cell. 2006 Feb 2/10/2006; 124(3): 631-44. PMID: 16469707, PMCID: PMC2443688, PII: S0092-8674(06)00008-0, DOI: 10.1016/j.cell.2005.11.043, ISSN: 0092-8674.

Davis MA, Reynolds AB. Blocked acinar development, E-cadherin reduction, and intraepithelial neoplasia upon ablation of p120-catenin in the mouse salivary gland. Dev. Cell. 2006 Jan; 10(1): 21-31. PMID: 16399075, PII: S1534-5807(05)00480-6, DOI: 10.1016/j.devcel.2005.12.004, ISSN: 1534-5807.

Soubry A, van Hengel J, Parthoens E, Colpaert C, Van Marck E, Waltregny D, Reynolds AB, van Roy F. Expression and nuclear location of the transcriptional repressor Kaiso is regulated by the tumor microenvironment. Cancer Res. 2005 Mar 3/15/2005; 65(6): 2224-33. PMID: 15781635, PII: 65/6/2224, DOI: 10.1158/0008-5472.CAN-04-2020, ISSN: 0008-5472.

Xia X, Brooks J, Campos-González R, Reynolds AB. Serine and threonine phospho-specific antibodies to p120-catenin. Hybrid. Hybridomics. 2004 Dec; 23(6): 343-51. PMID: 15684660, DOI: 10.1089/hyb.2004.23.343, ISSN: 1536-8599.

Reynolds AB, Carnahan RH. Regulation of cadherin stability and turnover by p120ctn: implications in disease and cancer. Semin. Cell Dev. Biol. 2004 Dec; 15(6): 657-63. PMID: 15561585, PII: S1084-9521(04)00089-8, DOI: 10.1016/j.semcdb.2004.09.003, ISSN: 1084-9521.

Reynolds AB, Roczniak-Ferguson A. Emerging roles for p120-catenin in cell adhesion and cancer. Oncogene. 2004 Oct 10/18/2004; 23(48): 7947-56. PMID: 15489912, PII: 1208161, DOI: 10.1038/sj.onc.1208161, ISSN: 0950-9232.

Kowalczyk AP, Reynolds AB. Protecting your tail: regulation of cadherin degradation by p120-catenin. Curr. Opin. Cell Biol. 2004 Oct; 16(5): 522-7. PMID: 15363802, PII: S0955-0674(04)00096-1, DOI: 10.1016/j.ceb.2004.07.001, ISSN: 0955-0674.

Gu J, Xia X, Yan P, Liu H, Podust VN, Reynolds AB, Fanning E. Cell cycle-dependent regulation of a human DNA helicase that localizes in DNA damage foci. Mol. Biol. Cell [print-electronic]. 2004 Jul; 15(7): 3320-32. PMID: 15146062, PMCID: PMC452586, PII: E04-03-0227, DOI: 10.1091/mbc.E04-03-0227, ISSN: 1059-1524.

Mariner DJ, Davis MA, Reynolds AB. EGFR signaling to p120-catenin through phosphorylation at Y228. J. Cell. Sci [print-electronic]. 2004 Mar 3/15/2004; 117(Pt 8): 1339-50. PMID: 14996911, PII: jcs.01001, DOI: 10.1242/jcs.01001, ISSN: 0021-9533.

Yanagisawa M, Kaverina IN, Wang A, Fujita Y, Reynolds AB, Anastasiadis PZ. A novel interaction between kinesin and p120 modulates p120 localization and function. J. Biol. Chem [print-electronic]. 2004 Mar 3/5/2004; 279(10): 9512-21. PMID: 14676216, PII: M310895200, DOI: 10.1074/jbc.M310895200, ISSN: 0021-9258.

Davis MA, Ireton RC, Reynolds AB. A core function for p120-catenin in cadherin turnover. J. Cell Biol. 2003 Nov 11/10/2003; 163(3): 525-34. PMID: 14610055, PMCID: PMC2173649, PII: jcb.200307111, DOI: 10.1083/jcb.200307111, ISSN: 0021-9525.

Roczniak-Ferguson A, Reynolds AB. Regulation of p120-catenin nucleocytoplasmic shuttling activity. J. Cell. Sci [print-electronic]. 2003 Oct 10/15/2003; 116(Pt 20): 4201-12. PMID: 12953069, PII: jcs.00724, DOI: 10.1242/jcs.00724, ISSN: 0021-9533.

Yoon HG, Chan DW, Reynolds AB, Qin J, Wong J. N-CoR mediates DNA methylation-dependent repression through a methyl CpG binding protein Kaiso. Mol. Cell. 2003 Sep; 12(3): 723-34. PMID: 14527417, PII: S1097276503003174, ISSN: 1097-2765.

Xia X, Mariner DJ, Reynolds AB. Adhesion-associated and PKC-modulated changes in serine/threonine phosphorylation of p120-catenin. Biochemistry. 2003 Aug 8/5/2003; 42(30): 9195-204. PMID: 12885254, DOI: 10.1021/bi034597h, ISSN: 0006-2960.

Goodwin M, Kovacs EM, Thoreson MA, Reynolds AB, Yap AS. Minimal mutation of the cytoplasmic tail inhibits the ability of E-cadherin to activate Rac but not phosphatidylinositol 3-kinase: direct evidence of a role for cadherin-activated Rac signaling in adhesion and contact formation. J. Biol. Chem [print-electronic]. 2003 Jun 6/6/2003; 278(23): 20533-9. PMID: 12672818, PII: M213171200, DOI: 10.1074/jbc.M213171200, ISSN: 0021-9258.

Thoreson MA, Reynolds AB. Altered expression of the catenin p120 in human cancer: implications for tumor progression. Differentiation. 2002 Dec; 70(9-10): 583-9. PMID: 12492499, PII: S0301-4681(09)60467-9, DOI: 10.1046/j.1432-0436.2002.700911.x, ISSN: 0301-4681.

Ireton RC, Davis MA, van Hengel J, Mariner DJ, Barnes K, Thoreson MA, Anastasiadis PZ, Matrisian L, Bundy LM, Sealy L, Gilbert B, van Roy F, Reynolds AB. A novel role for p120 catenin in E-cadherin function. J. Cell Biol [print-electronic]. 2002 Nov 11/11/2002; 159(3): 465-76. PMID: 12427869, PMCID: PMC2173073, PII: jcb.200205115, DOI: 10.1083/jcb.200205115, ISSN: 0021-9525.

Daniel JM, Spring CM, Crawford HC, Reynolds AB, Baig A. The p120(ctn)-binding partner Kaiso is a bi-modal DNA-binding protein that recognizes both a sequence-specific consensus and methylated CpG dinucleotides. Nucleic Acids Res. 2002 Jul 7/1/2002; 30(13): 2911-9. PMID: 12087177, PMCID: PMC117053, ISSN: 1362-4962.

Anastasiadis PZ, Reynolds AB. Regulation of Rho GTPases by p120-catenin. Curr. Opin. Cell Biol. 2001 Oct; 13(5): 604-10. PMID: 11544030, PII: S0955-0674(00)00258-1, ISSN: 0955-0674.

Mariner DJ, Anastasiadis P, Keilhack H, Böhmer FD, Wang J, Reynolds AB. Identification of Src phosphorylation sites in the catenin p120ctn. J. Biol. Chem [print-electronic]. 2001 Jul 7/27/2001; 276(30): 28006-13. PMID: 11382764, PII: M102443200, DOI: 10.1074/jbc.M102443200, ISSN: 0021-9258.

Daniel JM, Ireton RC, Reynolds AB. Monoclonal antibodies to Kaiso: a novel transcription factor and p120ctn-binding protein. Hybridoma. 2001 Jun; 20(3): 159-66. PMID: 11461664, DOI: 10.1089/027245701750293484, ISSN: 0272-457X.

Anastasiadis PZ, Moon SY, Thoreson MA, Mariner DJ, Crawford HC, Zheng Y, Reynolds AB. Inhibition of RhoA by p120 catenin. Nat. Cell Biol. 2000 Sep; 2(9): 637-44. PMID: 10980705, DOI: 10.1038/35023588, ISSN: 1465-7392.

Zondag GC, Reynolds AB, Moolenaar WH. Receptor protein-tyrosine phosphatase RPTPmu binds to and dephosphorylates the catenin p120(ctn). J. Biol. Chem. 2000 Apr 4/14/2000; 275(15): 11264-9. PMID: 10753936, ISSN: 0021-9258.

Mariner DJ, Wang J, Reynolds AB. ARVCF localizes to the nucleus and adherens junction and is mutually exclusive with p120(ctn) in E-cadherin complexes. J. Cell. Sci. 2000 Apr; 113 ( Pt 8): 1481-90. PMID: 10725230, ISSN: 0021-9533.

Anastasiadis PZ, Reynolds AB. The p120 catenin family: complex roles in adhesion, signaling and cancer. J. Cell. Sci. 2000 Apr; 113 ( Pt 8): 1319-34. PMID: 10725216, ISSN: 0021-9533.

Thoreson MA, Anastasiadis PZ, Daniel JM, Ireton RC, Wheelock MJ, Johnson KR, Hummingbird DK, Reynolds AB. Selective uncoupling of p120(ctn) from E-cadherin disrupts strong adhesion. J. Cell Biol. 2000 Jan 1/10/2000; 148(1): 189-202. PMID: 10629228, PMCID: PMC2156209, ISSN: 0021-9525.

Jin F, Reynolds AB, Hines MD, Jensen PJ, Johnson KR, Wheelock MJ. Src induces morphological changes in A431 cells that resemble epidermal differentiation through an SH3- and Ras-independent pathway. J. Cell. Sci. 1999 Sep; 112 ( Pt 17): 2913-24. PMID: 10444386, ISSN: 0021-9533.

Mariner DJ, Sirotkin H, Daniel JM, Lindman BR, Mernaugh RL, Patten AK, Thoreson MA, Reynolds AB. Production and characterization of monoclonal antibodies to ARVCF. Hybridoma. 1999 Aug; 18(4): 343-9. PMID: 10571264, ISSN: 0272-457X.

Aono S, Nakagawa S, Reynolds AB, Takeichi M. P120(ctn) acts as an inhibitory regulator of cadherin function in colon carcinoma cells. J. Cell Biol. 1999 May 5/3/1999; 145(3): 551-62. PMID: 10225956, PMCID: PMC2185070, ISSN: 0021-9525.

Daniel JM, Reynolds AB. The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger transcription factor. Mol. Cell. Biol. 1999 May; 19(5): 3614-23. PMID: 10207085, PMCID: PMC84161, ISSN: 0270-7306.

Paulson AF, Fang X, Ji H, Reynolds AB, McCrea PD. Misexpression of the catenin p120(ctn)1A perturbs Xenopus gastrulation but does not elicit Wnt-directed axis specification. Dev. Biol. 1999 Mar 3/15/1999; 207(2): 350-63. PMID: 10068468, PII: S0012-1606(98)99158-8, DOI: 10.1006/dbio.1998.9158, ISSN: 0012-1606.

Gold JS, Reynolds AB, Rimm DL. Loss of p120ctn in human colorectal cancer predicts metastasis and poor survival. Cancer Lett. 1998 Oct 10/23/1998; 132(1-2): 193-201. PMID: 10397474, PII: S0304-3835(98)00190-6, ISSN: 0304-3835.

Sheng H, Shao J, Williams CS, Pereira MA, Taketo MM, Oshima M, Reynolds AB, Washington MK, DuBois RN, Beauchamp RD. Nuclear translocation of beta-catenin in hereditary and carcinogen-induced intestinal adenomas. Carcinogenesis. 1998 Apr; 19(4): 543-9. PMID: 9600336, ISSN: 0143-3334.

Wu J, Mariner DJ, Thoreson MA, Reynolds AB. Production and characterization of monoclonal antibodies to the catenin p120ctn. Hybridoma. 1998 Apr; 17(2): 175-83. PMID: 9627058, ISSN: 0272-457X.

Dillon DA, D'Aquila T, Reynolds AB, Fearon ER, Rimm DL. The expression of p120ctn protein in breast cancer is independent of alpha- and beta-catenin and E-cadherin. Am. J. Pathol. 1998 Jan; 152(1): 75-82. PMID: 9422525, PMCID: PMC1858125, ISSN: 0002-9440.

Keirsebilck A, van Hengel J, Staes K, Bonne S, Nollet F, Reynolds AB, van Roy F. Molecular cloning of the human p120ctn catenin gene: expression of multiple alternatively spliced isoforms. Genomics. 1998; 50: 129-46.

Daniel JM, Reynolds AB. Tyrosine phosphorylation and cadherin/catenin function. Bioessays. 1997 Oct; 19(10): 883-91. PMID: 9363682, DOI: 10.1002/bies.950191008, ISSN: 0265-9247.

Fornerod, M., van Deursen, J., van Baal, S., Reynolds, A., Davis, D., Murti, G.K., Fransen, J., Grosveld, G. The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup2l4 and a novel nuclear pore component Nup88. EMBO J. 1997; 16: 807-16.

Reynolds, A; Daniel, J.. P120ctn: a Src-substrate turned catenin. In Cytoskeletal-Membrane Interactions and Signals Transduction, (eds. P. Cowin and M. Klymiowsky, Landes Bioscience, USA. Chapt. 3). 1997.

Reynolds AB, Daniel JM, Mo YY, Wu J, Zhang Z. The novel catenin p120cas binds classical cadherins and induces an unusual morphological phenotype in NIH3T3 fibroblasts. Exp. Cell Res. 1996 Jun 6/15/1996; 225(2): 328-37. PMID: 8660921, PII: S0014-4827(96)90183-4, DOI: 10.1006/excr.1996.0183, ISSN: 0014-4827.

Mo YY, Reynolds AB. Identification of murine p120 isoforms and heterogeneous expression of p120cas isoforms in human tumor cell lines. Cancer Res. 1996 Jun 6/1/1996; 56(11): 2633-40. PMID: 8653709, ISSN: 0008-5472.

Reynolds AB, Jenkins NA, Gilbert DJ, Copeland NG, Shapiro DN, Wu J, Daniel JM. The gene encoding p120cas, a novel catenin, localizes on human chromosome 11q11 (CTNND) and mouse chromosome 2 (Catns). Genomics. 1996 Jan 1/1/1996; 31(1): 127-9. PMID: 8808291, PII: S0888-7543(96)90020-8, DOI: 10.1006/geno.1996.0020, ISSN: 0888-7543.

Daniel JM, Reynolds AB. The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha-catenin. Mol. Cell. Biol. 1995 Sep; 15(9): 4819-24. PMID: 7651399, PMCID: PMC230726, ISSN: 0270-7306.

Parham DM, Reynolds AB, Webber BL. Use of monoclonal antibody 1H1, anticortactin, to distinguish normal and neoplastic smooth muscle cells: comparison with anti-alpha-smooth muscle actin and antimuscle-specific actin. Hum. Pathol. 1995 Jul; 26(7): 776-83. PMID: 7628851, ISSN: 0046-8177.

Reynolds AB, Daniel J, McCrea PD, Wheelock MJ, Wu J, Zhang Z. Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes. Mol. Cell. Biol. 1994 Dec; 14(12): 8333-42. PMID: 7526156, PMCID: PMC359372, ISSN: 0270-7306.

Peifer M, Berg S, Reynolds AB. A repeating amino acid motif shared by proteins with diverse cellular roles [letter]. Cell. 1994 Mar 3/11/1994; 76(5): 789-91. PMID: 7907279, PII: 0092-8674(94)90353-0, ISSN: 0092-8674.

Flynn DC, Leu TH, Reynolds AB, Parsons JT. Identification and sequence analysis of cDNAs encoding a 110-kilodalton actin filament-associated pp60src substrate. Mol. Cell. Biol. 1993 Dec; 13(12): 7892-900. PMID: 8247004, PMCID: PMC364861, ISSN: 0270-7306.

Reynolds AB, Herbert L, Cleveland JL, Berg ST, Gaut JR. P120, a novel substrate of protein tyrosine kinase receptors and of p60v-src, is related to cadherin-binding factors beta-catenin, plakoglobin and armadillo. Oncogene. 1992 Dec; 7(12): 2439-45. PMID: 1334250, ISSN: 0950-9232.

Wong S, Reynolds AB, Papkoff J. Platelet activation leads to increased c-src kinase activity and association of c-src with an 85-kDa tyrosine phosphoprotein. Oncogene. 1992 Dec; 7(12): 2407-15. PMID: 1281303, ISSN: 0950-9232.

Schaller MD, Borgman CA, Cobb BS, Vines RR, Reynolds AB, Parsons JT. Pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions. Proc. Natl. Acad. Sci. U.S.A. 1992 Jun 6/1/1992; 89(11): 5192-6. PMID: 1594631, PMCID: PMC49256, ISSN: 0027-8424.

Cobb BS, Payne DM, Reynolds AB, Parsons JT. Regulation of the oncogenic activity of the cellular src protein requires the correct spacing between the kinase domain and the C-terminal phosphorylated tyrosine (Tyr-527). Mol. Cell. Biol. 1991 Dec; 11(12): 5832-8. PMID: 1719372, PMCID: PMC361728, ISSN: 0270-7306.

Wu H, Reynolds AB, Kanner SB, Vines RR, Parsons JT. Identification and characterization of a novel cytoskeleton-associated pp60src substrate. Mol. Cell. Biol. 1991 Oct; 11(10): 5113-24. PMID: 1922035, PMCID: PMC361526, ISSN: 0270-7306.

Kanner SB, Reynolds AB, Wang HC, Vines RR, Parsons JT. The SH2 and SH3 domains of pp60src direct stable association with tyrosine phosphorylated proteins p130 and p110. EMBO J. 1991 Jul; 10(7): 1689-98. PMID: 1710979, PMCID: PMC452840, ISSN: 0261-4189.

Downing JR, Reynolds AB. PDGF, CSF-1, and EGF induce tyrosine phosphorylation of p120, a pp60src transformation-associated substrate. Oncogene. 1991 Apr; 6(4): 607-13. PMID: 1851549, ISSN: 0950-9232.

Kanner SB, Reynolds AB, Parsons JT. Tyrosine phosphorylation of a 120-kilodalton pp60src substrate upon epidermal growth factor and platelet-derived growth factor receptor stimulation and in polyomavirus middle-T-antigen-transformed cells. Mol. Cell. Biol. 1991 Feb; 11(2): 713-20. PMID: 1703631, PMCID: PMC359722, ISSN: 0270-7306.

Kanner SB, Reynolds AB, Vines RR, Parsons JT. Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases. Proc. Natl. Acad. Sci. U.S.A. 1990 May; 87(9): 3328-32. PMID: 2110361, PMCID: PMC53893, ISSN: 0027-8424.

Kozma LM, Reynolds AB, Weber MJ. Glycoprotein tyrosine phosphorylation in Rous sarcoma virus-transformed chicken embryo fibroblasts. Mol. Cell. Biol. 1990 Feb; 10(2): 837-41. PMID: 1689002, PMCID: PMC360886, ISSN: 0270-7306.

Reynolds AB, Kanner SB, Wang HC, Parsons JT. Stable association of activated pp60src with two tyrosine-phosphorylated cellular proteins. Mol. Cell. Biol. 1989 Sep; 9(9): 3951-8. PMID: 2476666, PMCID: PMC362457, ISSN: 0270-7306.

Kanner SB, Reynolds AB, Parsons JT. Immunoaffinity purification of tyrosine-phosphorylated cellular proteins. J. Immunol. Methods. 1989 Jun 6/2/1989; 120(1): 115-24. PMID: 2471744, ISSN: 0022-1759.

Reynolds AB, Thomas TS, Wilson WL, Oliphant G. Concentration of acrosome stabilizing factor (ASF) in rabbit epididymal fluid and species specificity of anti-ASF antibodies. Biol. Reprod. 1989 Mar; 40(3): 673-80. PMID: 2758096, ISSN: 0006-3363.

Kanner SB, Gilmer TM, Reynolds AB, Parsons JT. Novel tyrosine phosphorylations accompany the activation of pp60c-src during chemical carcinogenesis. Oncogene. 1989 Mar; 4(3): 295-300. PMID: 2468123, ISSN: 0950-9232.

Reynolds AB, Roesel DJ, Kanner SB, Parsons JT. Transformation-specific tyrosine phosphorylation of a novel cellular protein in chicken cells expressing oncogenic variants of the avian cellular src gene. Mol. Cell. Biol. 1989 Feb; 9(2): 629-38. PMID: 2469003, PMCID: PMC362640, ISSN: 0270-7306.

Potts WM, Reynolds AB, Lansing TJ, Parsons JT. Activation of pp60c-src transforming potential by mutations altering the structure of an amino terminal domain containing residues 90-95. Oncogene Res. 1988; 3(4): 343-55. PMID: 2465527, ISSN: 0890-6467.

Reynolds AB, Vila J, Lansing TJ, Potts WM, Weber MJ, Parsons JT. Activation of the oncogenic potential of the avian cellular src protein by specific structural alteration of the carboxy terminus. EMBO J. 1987 Aug; 6(8): 2359-64. PMID: 2822389, PMCID: PMC553640, ISSN: 0261-4189.

Wilson WL, Reynolds AB, Marta J, Oliphant G. Correlation of conformational changes in the acrosome stabilizing factor (ASF) with its biological activity. Biol. Reprod. 1987 May; 36(4): 1069-77. PMID: 3593851, ISSN: 0006-3363.

Reynolds AB, Oliphant G. Quantitation of the rabbit sperm acrosome stabilizing factor utilizing a sensitive immunoradiometric assay. Biol. Reprod. 1986 Oct; 35(3): 705-15. PMID: 3790670, ISSN: 0006-3363.

Thomas TS, Wilson WL, Reynolds AB, Oliphant G. Chemical and physical characterization of rabbit sperm acrosome stabilizing factor. Biol. Reprod. 1986 Oct; 35(3): 691-703. PMID: 3790669, ISSN: 0006-3363.

Oliphant G, Reynolds AB, Thomas TS. Sperm surface components involved in the control of the acrosome reaction. Am. J. Anat. 1985 Nov; 174(3): 269-83. PMID: 4072942, DOI: 10.1002/aja.1001740308, ISSN: 0002-9106.

Reynolds AB, Oliphant G. Production and characterization of monoclonal antibodies to the sperm acrosome stabilizing factor (ASF): utilization for purification and molecular analysis of ASF. Biol. Reprod. 1984 Apr; 30(3): 775-86. PMID: 6722244, ISSN: 0006-3363.

Thomas TS, Reynolds AB, Oliphant G. Evaluation of the site of synthesis of rabbit sperm acrosome stabilizing factor using immunocytochemical and metabolic labeling techniques. Biol. Reprod. 1984 Apr; 30(3): 693-705. PMID: 6372878, ISSN: 0006-3363.