Robert J. Coffey, Jr., M.D.


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Faculty Appointments
Professor of Medicine Ingram Professor of Cancer ResearchProfessor of Cell and Developmental Biology
M.D., Medicine, Georgetown University, Washington, District of ColumbiaA.B., Political Science, Princeton University, Princeton, New Jersey
Office Address
10415-E MRB IV -0441
2213 Garland Avenue
Nashville, TN 37232
Research Description
The focus of research within the Coffey lab is the study of the role of the EGF receptor (EGFR) and its ligands in GI neoplasia. The lab has a particular interest in the trafficking of EGFR ligands in polarizing colonic epithelial cells. Naked2 has been identified as a critical regulator of basolateral trafficking of TGF-alpha, a major EGFR ligand. Naked2 is a known antagonist of Wnt-beta-catenin signaling so Naked2 provides a point of convergence between EGFR and Wnt signaling. This work has led to the identification of a new mode of EGFR ligand signaling via exosomes and the development of FAVS (fluorescent-activated vesicle sorting) to isolate and characterize cellular organelles. The lab has recently found that Lrig1, an inducible negative regulator of the EGFR, marks proliferative and quiescent intestinal stem cells, and acts as a tumor suppressor.
Research Keywords
TGF-alpha, EGFR, Naked2, Epithelial Polarity, Vesicle Trafficking, GI Cancer, Menetrier's disease
Singh B, Bogatcheva G, Starchenko A, Sinnaeve J, Lapierre LA, Williams JA, Goldenring JR, Coffey RJ. Induction of lateral lumens through disruption of a monoleucine-based basolateral-sorting motif in betacellulin. J. Cell. Sci [print-electronic]. 2015 Sep 9/15/2015; 128(18): 3444-55. PMID: 26272915, PMCID: PMC4582402, PII: jcs.170852, DOI: 10.1242/jcs.170852, ISSN: 1477-9137.

Kondo J, Powell AE, Wang Y, Musser MA, Southard-Smith EM, Franklin JL, Coffey RJ. LRIG1 Regulates Ontogeny of Smooth Muscle-Derived Subsets of Interstitial Cells of Cajal in Mice. Gastroenterology [print-electronic]. 2015 Aug; 149(2): 407-19.e8. PMID: 25921371, PMCID: PMC4527342, PII: S0016-5085(15)00574-0, DOI: 10.1053/j.gastro.2015.04.018, ISSN: 1528-0012.

McKinley ET, Watchmaker JM, Chakravarthy AB, Meyerhardt JA, Engelman JA, Walker RC, Washington MK, Coffey RJ, Manning HC. [(18)F]-FLT PET to predict early response to neoadjuvant therapy in KRAS wild-type rectal cancer: a pilot study. Ann Nucl Med [print-electronic]. 2015 Jul; 29(6): 535-42. PMID: 25899481, PMCID: PMC4503488, DOI: 10.1007/s12149-015-0974-6, ISSN: 1864-6433.

Gonzalez RS, Cates JM, Washington MK, Beauchamp RD, Coffey RJ, Shi C. Adenoma-like adenocarcinoma: a subtype of colorectal carcinoma with good prognosis, deceptive appearance on biopsy and frequent KRAS mutation. Histopathology [print-electronic]. 2015 Apr 4/24/2015; PMID: 25913616, PMCID: PMC4619179, DOI: 10.1111/his.12725, ISSN: 1365-2559.

Wang Y, Shi C, Lu Y, Poulin EJ, Franklin JL, Coffey RJ. Loss of Lrig1 leads to expansion of Brunner glands followed by duodenal adenomas with gastric metaplasia. Am. J. Pathol. 2015 Apr; 185(4): 1123-34. PMID: 25794708, PMCID: PMC4380843, PII: S0002-9440(15)00022-X, DOI: 10.1016/j.ajpath.2014.12.014, ISSN: 1525-2191.

Simmons AJ, Banerjee A, McKinley ET, Scurrah CR, Herring CA, Gewin LS, Masuzaki R, Karp SJ, Franklin JL, Gerdes MJ, Irish JM, Coffey RJ, Lau KS. Cytometry-based single-cell analysis of intact epithelial signaling reveals MAPK activation divergent from TNF-a-induced apoptosis in vivo. Mol. Syst. Biol. 2015; 11(10): 835. PMID: 26519361, PMCID: PMC4631206, ISSN: 1744-4292.

Quesenberry PJ, Aliotta J, Camussi G, Abdel-Mageed AB, Wen S, Goldberg L, Zhang HG, Tetta C, Franklin J, Coffey RJ, Danielson K, Subramanya V, Ghiran I, Das S, Chen CC, Pusic KM, Pusic AD, Chatterjee D, Kraig RP, Balaj L, Dooner M. Potential functional applications of extracellular vesicles: a report by the NIH Common Fund Extracellular RNA Communication Consortium. J Extracell Vesicles. 2015; 4: 27575. PMID: 26320942, PMCID: PMC4553260, PII: 27575, ISSN: 2001-3078.

Patton JG, Franklin JL, Weaver AM, Vickers K, Zhang B, Coffey RJ, Ansel KM, Blelloch R, Goga A, Huang B, L'Etoille N, Raffai RL, Lai CP, Krichevsky AM, Mateescu B, Greiner VJ, Hunter C, Voinnet O, McManus MT. Biogenesis, delivery, and function of extracellular RNA. J Extracell Vesicles. 2015; 4: 27494. PMID: 26320939, PMCID: PMC4553266, PII: 27494, ISSN: 2001-3078.

Cha DJ, Franklin JL, Dou Y, Liu Q, Higginbotham JN, Demory Beckler M, Weaver AM, Vickers K, Prasad N, Levy S, Zhang B, Coffey RJ, Patton JG. KRAS-dependent sorting of miRNA to exosomes. Elife. 2015; 4: e07197. PMID: 26132860, PMCID: PMC4510696, DOI: 10.7554/eLife.07197, ISSN: 2050-084X.

French CL, Ye F, Revetta F, Zhang B, Coffey RJ, Washington MK, Deane NG, Beauchamp RD, Weaver AM. Linking patient outcome to high throughput protein expression data identifies novel regulators of colorectal adenocarcinoma aggressiveness. F1000Res. 2015; 4: 99. PMID: 26097693, PMCID: PMC4457132, DOI: 10.12688/f1000research.6388.1, ISSN: 2046-1402.

Poulin EJ, Powell AE, Wang Y, Li Y, Franklin JL, Coffey RJ. Using a new Lrig1 reporter mouse to assess differences between two Lrig1 antibodies in the intestine. Stem Cell Res [print-electronic]. 2014 Nov; 13(3 Pt A): 422-30. PMID: 25460603, PMCID: PMC4320017, PII: S1873-5061(14)00104-4, DOI: 10.1016/j.scr.2014.09.002, ISSN: 1876-7753.

Zhang B, Wang J, Wang X, Zhu J, Liu Q, Shi Z, Chambers MC, Zimmerman LJ, Shaddox KF, Kim S, Davies SR, Wang S, Wang P, Kinsinger CR, Rivers RC, Rodriguez H, Townsend RR, Ellis MJ, Carr SA, Tabb DL, Coffey RJ, Slebos RJ, Liebler DC, . Proteogenomic characterization of human colon and rectal cancer. Nature [print-electronic]. 2014 Sep 9/18/2014; 513(7518): 382-7. PMID: 25043054, PMCID: PMC4249766, PII: nature13438, DOI: 10.1038/nature13438, ISSN: 1476-4687.

Markham NO, Doll CA, Dohn MR, Miller RK, Yu H, Coffey RJ, McCrea PD, Gamse JT, Reynolds AB. DIPA-family coiled-coils bind conserved isoform-specific head domain of p120-catenin family: potential roles in hydrocephalus and heterotopia. Mol. Biol. Cell [print-electronic]. 2014 Sep 9/1/2014; 25(17): 2592-603. PMID: 25009281, PMCID: PMC4148249, PII: mbc.E13-08-0492, DOI: 10.1091/mbc.E13-08-0492, ISSN: 1939-4586.

Powell AE, Vlacich G, Zhao ZY, McKinley ET, Washington MK, Manning HC, Coffey RJ. Inducible loss of one Apc allele in Lrig1-expressing progenitor cells results in multiple distal colonic tumors with features of familial adenomatous polyposis. Am. J. Physiol. Gastrointest. Liver Physiol [print-electronic]. 2014 Jul 7/1/2014; 307(1): G16-23. PMID: 24833705, PMCID: PMC4080164, PII: ajpgi.00358.2013, DOI: 10.1152/ajpgi.00358.2013, ISSN: 1522-1547.

Kawasaki K, Freimuth J, Meyer DS, Lee MM, Tochimoto-Okamoto A, Benzinou M, Clermont FF, Wu G, Roy R, Letteboer TG, Ploos van Amstel JK, Giraud S, Dupuis-Girod S, Lesca G, Westermann CJ, Coffey RJ, Akhurst RJ. Genetic variants of Adam17 differentially regulate TGFß signaling to modify vascular pathology in mice and humans. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2014 May 5/27/2014; 111(21): 7723-8. PMID: 24812125, PMCID: PMC4040598, PII: 1318761111, DOI: 10.1073/pnas.1318761111, ISSN: 1091-6490.

Li C, Ma H, Wang Y, Cao Z, Graves-Deal R, Powell AE, Starchenko A, Ayers GD, Washington MK, Kamath V, Desai K, Gerdes MJ, Solnica-Krezel L, Coffey RJ. Excess PLAC8 promotes an unconventional ERK2-dependent EMT in colon cancer. J. Clin. Invest [print-electronic]. 2014 May; 124(5): 2172-87. PMID: 24691442, PMCID: PMC4001536, PII: 71103, DOI: 10.1172/JCI71103, ISSN: 1558-8238.

Hight MR, Cheung YY, Nickels ML, Dawson ES, Zhao P, Saleh S, Buck JR, Tang D, Washington MK, Coffey RJ, Manning HC. A peptide-based positron emission tomography probe for in vivo detection of caspase activity in apoptotic cells. Clin. Cancer Res [print-electronic]. 2014 Apr 4/15/2014; 20(8): 2126-35. PMID: 24573549, PMCID: PMC3989451, PII: 1078-0432.CCR-13-2444, DOI: 10.1158/1078-0432.CCR-13-2444, ISSN: 1078-0432.

Singh B, Coffey RJ. From wavy hair to naked proteins: the role of transforming growth factor alpha in health and disease. Semin. Cell Dev. Biol [print-electronic]. 2014 Apr; 28: 12-21. PMID: 24631356, PMCID: PMC4105142, PII: S1084-9521(14)00031-7, DOI: 10.1016/j.semcdb.2014.03.003, ISSN: 1096-3634.

Ray KC, Moss ME, Franklin JL, Weaver CJ, Higginbotham J, Song Y, Revetta FL, Blaine SA, Bridges LR, Guess KE, Coffey RJ, Crawford HC, Washington MK, Means AL. Heparin-binding epidermal growth factor-like growth factor eliminates constraints on activated Kras to promote rapid onset of pancreatic neoplasia. Oncogene [print-electronic]. 2014 Feb 2/13/2014; 33(7): 823-31. PMID: 23376846, PMCID: PMC3929321, PII: onc20133, DOI: 10.1038/onc.2013.3, ISSN: 1476-5594.

Singh B, Coffey RJ. Trafficking of epidermal growth factor receptor ligands in polarized epithelial cells. Annu. Rev. Physiol [print-electronic]. 2014; 76: 275-300. PMID: 24215440, PMCID: PMC4180094, DOI: 10.1146/annurev-physiol-021113-170406, ISSN: 1545-1585.

McKinley ET, Zhao P, Coffey RJ, Washington MK, Manning HC. 3'-Deoxy-3'-[18F]-Fluorothymidine PET imaging reflects PI3K-mTOR-mediated pro-survival response to targeted therapy in colorectal cancer. PLoS ONE. 2014; 9(9): e108193. PMID: 25247710, PMCID: PMC4172755, PII: PONE-D-14-08668, DOI: 10.1371/journal.pone.0108193, ISSN: 1932-6203.

Franklin JL, Rankin CR, Levy S, Snoddy JR, Zhang B, Washington MK, Thomson JM, Whitehead RH, Coffey RJ. Malignant transformation of colonic epithelial cells by a colon-derived long noncoding RNA. Biochem. Biophys. Res. Commun [print-electronic]. 2013 Oct 10/11/2013; 440(1): 99-104. PMID: 24045012, PMCID: PMC3875348, PII: S0006-291X(13)01511-8, DOI: 10.1016/j.bbrc.2013.09.040, ISSN: 1090-2104.

Singh B, Bogatcheva G, Washington MK, Coffey RJ. Transformation of polarized epithelial cells by apical mistrafficking of epiregulin. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 May 5/28/2013; 110(22): 8960-5. PMID: 23671122, PMCID: PMC3670353, PII: 1305508110, DOI: 10.1073/pnas.1305508110, ISSN: 1091-6490.

Wang Y, Poulin EJ, Coffey RJ. LRIG1 is a triple threat: ERBB negative regulator, intestinal stem cell marker and tumour suppressor. Br. J. Cancer [print-electronic]. 2013 May 5/14/2013; 108(9): 1765-70. PMID: 23558895, PMCID: PMC3658528, PII: bjc2013138, DOI: 10.1038/bjc.2013.138, ISSN: 1532-1827.

Shifrin DA, Demory Beckler M, Coffey RJ, Tyska MJ. Extracellular vesicles: communication, coercion, and conditioning. Mol. Biol. Cell. 2013 May; 24(9): 1253-9. PMID: 23630232, PMCID: PMC3639038, PII: 24/9/1253, DOI: 10.1091/mbc.E12-08-0572, ISSN: 1939-4586.

Washington MK, Powell AE, Sullivan R, Sundberg JP, Wright N, Coffey RJ, Dove WF. Pathology of rodent models of intestinal cancer: progress report and recommendations. Gastroenterology [print-electronic]. 2013 Apr; 144(4): 705-17. PMID: 23415801, PMCID: PMC3660997, PII: S0016-5085(13)00166-2, DOI: 10.1053/j.gastro.2013.01.067, ISSN: 1528-0012.

McKinley ET, Smith RA, Zhao P, Fu A, Saleh SA, Uddin MI, Washington MK, Coffey RJ, Manning HC. 3'-Deoxy-3'-18F-fluorothymidine PET predicts response to (V600E)BRAF-targeted therapy in preclinical models of colorectal cancer. J. Nucl. Med [print-electronic]. 2013 Mar; 54(3): 424-30. PMID: 23341544, PMCID: PMC3633462, PII: jnumed.112.108456, DOI: 10.2967/jnumed.112.108456, ISSN: 1535-5667.

Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Mol. Cell Proteomics [print-electronic]. 2013 Feb; 12(2): 343-55. PMID: 23161513, PMCID: PMC3567858, PII: M112.022806, DOI: 10.1074/mcp.M112.022806, ISSN: 1535-9484.

McKinley ET, Ayers GD, Smith RA, Saleh SA, Zhao P, Washington MK, Coffey RJ, Manning HC. Limits of [18F]-FLT PET as a biomarker of proliferation in oncology. PLoS ONE [print-electronic]. 2013; 8(3): e58938. PMID: 23554961, PMCID: PMC3598948, PII: PONE-D-12-24564, DOI: 10.1371/journal.pone.0058938, ISSN: 1932-6203.

Noto JM, Khizanishvili T, Chaturvedi R, Piazuelo MB, Romero-Gallo J, Delgado AG, Khurana SS, Sierra JC, Krishna US, Suarez G, Powell AE, Goldenring JR, Coffey RJ, Yang VW, Correa P, Mills JC, Wilson KT, Peek RM. Helicobacter pylori promotes the expression of Krüppel-like factor 5, a mediator of carcinogenesis, in vitro and in vivo. PLoS ONE [print-electronic]. 2013; 8(1): e54344. PMID: 23372710, PMCID: PMC3553174, PII: PONE-D-12-20127, DOI: 10.1371/journal.pone.0054344, ISSN: 1932-6203.

Tanksley JP, Chen X, Coffey RJ. NEDD4L is downregulated in colorectal cancer and inhibits canonical WNT signaling. PLoS ONE. 2013; 8(11): e81514. PMID: 24312311, PMCID: PMC3842946, PII: PONE-D-13-30141, DOI: 10.1371/journal.pone.0081514, ISSN: 1932-6203.

McKinley ET, Liu H, McDonald WH, Luo W, Zhao P, Coffey RJ, Hanks SK, Manning HC. Global phosphotyrosine proteomics identifies PKCd as a marker of responsiveness to Src inhibition in colorectal cancer. PLoS ONE. 2013; 8(11): e80207. PMID: 24260357, PMCID: PMC3832668, PII: PONE-D-13-29839, DOI: 10.1371/journal.pone.0080207, ISSN: 1932-6203.

Zhu J, Wang J, Shi Z, Franklin JL, Deane NG, Coffey RJ, Beauchamp RD, Zhang B. Deciphering genomic alterations in colorectal cancer through transcriptional subtype-based network analysis. PLoS ONE. 2013; 8(11): e79282. PMID: 24260186, PMCID: PMC3829853, PII: PONE-D-13-34057, DOI: 10.1371/journal.pone.0079282, ISSN: 1932-6203.

Nam KT, O'Neal RL, Coffey RJ, Finke PE, Barker N, Goldenring JR. Spasmolytic polypeptide-expressing metaplasia (SPEM) in the gastric oxyntic mucosa does not arise from Lgr5-expressing cells. Gut [print-electronic]. 2012 Dec; 61(12): 1678-85. PMID: 22198711, PMCID: PMC3767919, PII: gutjnl-2011-301193, DOI: 10.1136/gutjnl-2011-301193, ISSN: 1468-3288.

McKinley ET, Smith RA, Tanksley JP, Washington MK, Walker R, Coffey RJ, Manning HC. [18F]FLT-PET to predict pharmacodynamic and clinical response to cetuximab therapy in Ménétrier's disease. Ann Nucl Med [print-electronic]. 2012 Nov; 26(9): 757-63. PMID: 22821337, PMCID: PMC3543701, DOI: 10.1007/s12149-012-0636-x, ISSN: 1864-6433.

Kim TI, Poulin EJ, Blask E, Bukhalid R, Whitehead RH, Franklin JL, Coffey RJ. Myofibroblast keratinocyte growth factor reduces tight junctional integrity and increases claudin-2 levels in polarized Caco-2 cells. Growth Factors [print-electronic]. 2012 Oct; 30(5): 320-32. PMID: 22946653, PMCID: PMC3594790, DOI: 10.3109/08977194.2012.717076, ISSN: 1029-2292.

Nam KT, O'Neal R, Lee YS, Lee YC, Coffey RJ, Goldenring JR. Gastric tumor development in Smad3-deficient mice initiates from forestomach/glandular transition zone along the lesser curvature. Lab. Invest [print-electronic]. 2012 Jun; 92(6): 883-95. PMID: 22411066, PMCID: PMC3584162, PII: labinvest201247, DOI: 10.1038/labinvest.2012.47, ISSN: 1530-0307.

Shifrin DA, McConnell RE, Nambiar R, Higginbotham JN, Coffey RJ, Tyska MJ. Enterocyte microvillus-derived vesicles detoxify bacterial products and regulate epithelial-microbial interactions. Curr. Biol [print-electronic]. 2012 Apr 4/10/2012; 22(7): 627-31. PMID: 22386311, PMCID: PMC3326206, PII: S0960-9822(12)00171-6, DOI: 10.1016/j.cub.2012.02.022, ISSN: 1879-0445.

Powell AE, Wang Y, Li Y, Poulin EJ, Means AL, Washington MK, Higginbotham JN, Juchheim A, Prasad N, Levy SE, Guo Y, Shyr Y, Aronow BJ, Haigis KM, Franklin JL, Coffey RJ. The pan-ErbB negative regulator Lrig1 is an intestinal stem cell marker that functions as a tumor suppressor. Cell. 2012 Mar 3/30/2012; 149(1): 146-58. PMID: 22464327, PMCID: PMC3563328, PII: S0092-8674(12)00280-2, DOI: 10.1016/j.cell.2012.02.042, ISSN: 1097-4172.

Halvey PJ, Zhang B, Coffey RJ, Liebler DC, Slebos RJ. Proteomic consequences of a single gene mutation in a colorectal cancer model. J. Proteome Res [print-electronic]. 2012 Feb 2/3/2012; 11(2): 1184-95. PMID: 22103262, PMCID: PMC3271737, DOI: 10.1021/pr2009109, ISSN: 1535-3907.

Myers MV, Manning HC, Coffey RJ, Liebler DC. Protein expression signatures for inhibition of epidermal growth factor receptor-mediated signaling. Mol. Cell Proteomics [print-electronic]. 2012 Feb; 11(2): M111.015222. PMID: 22147731, PMCID: PMC3277773, PII: M111.015222, DOI: 10.1074/mcp.M111.015222, ISSN: 1535-9484.

Coffey RJ, Tanksley J. Pierre Ménétrier and his disease. Trans. Am. Clin. Climatol. Assoc. 2012; 123: 126-33; discussion 133. PMID: 23303980, PMCID: PMC3540591, ISSN: 0065-7778.

Gephart JD, Singh B, Higginbotham JN, Franklin JL, Gonzalez A, Fölsch H, Coffey RJ. Identification of a novel mono-leucine basolateral sorting motif within the cytoplasmic domain of amphiregulin. Traffic [print-electronic]. 2011 Dec; 12(12): 1793-804. PMID: 21917092, PMCID: PMC3886124, DOI: 10.1111/j.1600-0854.2011.01282.x, ISSN: 1600-0854.

Vlacich G, Coffey RJ. Resistance to EGFR-targeted therapy: a family affair. Cancer Cell. 2011 Oct 10/18/2011; 20(4): 423-5. PMID: 22014569, PMCID: PMC3616495, PII: S1535-6108(11)00365-5, DOI: 10.1016/j.ccr.2011.10.006, ISSN: 1878-3686.

Higginbotham JN, Demory Beckler M, Gephart JD, Franklin JL, Bogatcheva G, Kremers GJ, Piston DW, Ayers GD, McConnell RE, Tyska MJ, Coffey RJ. Amphiregulin exosomes increase cancer cell invasion. Curr. Biol [print-electronic]. 2011 May 5/10/2011; 21(9): 779-86. PMID: 21514161, PMCID: PMC3417320, PII: S0960-9822(11)00347-2, DOI: 10.1016/j.cub.2011.03.043, ISSN: 1879-0445.

Hu T, Li C, Cao Z, Van Raay TJ, Smith JG, Willert K, Solnica-Krezel L, Coffey RJ. Myristoylated Naked2 antagonizes Wnt-beta-catenin activity by degrading Dishevelled-1 at the plasma membrane. J. Biol. Chem [print-electronic]. 2010 Apr 4/30/2010; 285(18): 13561-8. PMID: 20177058, PMCID: PMC2859517, PII: M109.075945, DOI: 10.1074/jbc.M109.075945, ISSN: 1083-351X.

Wang Y, Coffey RJ, Osheroff N, Neufeld KL. Topoisomerase IIalpha binding domains of adenomatous polyposis coli influence cell cycle progression and aneuploidy. PLoS ONE. 2010; 5(4): e9994. PMID: 20368985, PMCID: PMC2848841, DOI: 10.1371/journal.pone.0009994, ISSN: 1932-6203.

Fiske WH, Tanksley J, Nam KT, Goldenring JR, Slebos RJC, Liebler DC, Abtahi AM, La Fleur B, Ayers JD, Lind CD, Washington MK and Coffey RJ. Efficacy of cetuximab in the treatment of Menetrier's disease. 2009.

Manning HC, Merchant NB, Foutch AC, Virostko JM, Wyatt SK, Shah C, McKinley ET, Xie J, Mutic NJ, Washington MK, LaFleur B, Tantawy MN, Peterson TE, Ansari MS, Baldwin RM, Rothenberg ML, Bornhop DJ, Gore JC, Coffey RJ. Molecular imaging of therapeutic response to epidermal growth factor receptor blockade in colorectal cancer. Clin. Cancer Res. 2008 Nov 11/15/2008; 14(22): 7413-22. PMID: 19010858, PMCID: PMC2657180, PII: 14/22/7413, DOI: 10.1158/1078-0432.CCR-08-0239, ISSN: 1078-0432.

Rodland KD, Bollinger N, Ippolito D, Opresko LK, Coffey RJ, Zangar R, Wiley HS. Multiple mechanisms are responsible for transactivation of the epidermal growth factor receptor in mammary epithelial cells. J. Biol. Chem [print-electronic]. 2008 Nov 11/14/2008; 283(46): 31477-87. PMID: 18782770, PMCID: PMC2581561, PII: M800456200, DOI: 10.1074/jbc.M800456200, ISSN: 0021-9258.

Cao Z, Li C, Higginbotham JN, Franklin JL, Tabb DL, Graves-Deal R, Hill S, Cheek K, Jerome WG, Lapierre LA, Goldenring JR, Ham AJ, Coffey RJ. Use of fluorescence-activated vesicle sorting for isolation of Naked2-associated, basolaterally targeted exocytic vesicles for proteomics analysis. Mol. Cell Proteomics [print-electronic]. 2008 Sep; 7(9): 1651-67. PMID: 18504258, PMCID: PMC2528074, PII: M700155-MCP200, DOI: 10.1074/mcp.M700155-MCP200, ISSN: 1535-9484.

Kim I, Fu Y, Hui K, Moeckel G, Mai W, Li C, Liang D, Zhao P, Ma J, Chen XZ, George AL, Coffey RJ, Feng ZP, Wu G. Fibrocystin/polyductin modulates renal tubular formation by regulating polycystin-2 expression and function. J. Am. Soc. Nephrol [print-electronic]. 2008 Mar; 19(3): 455-68. PMID: 18235088, PMCID: PMC2391052, PII: ASN.2007070770, DOI: 10.1681/ASN.2007070770, ISSN: 1533-3450.

Merchant NB, Voskresensky I, Rogers CM, Lafleur B, Dempsey PJ, Graves-Deal R, Revetta F, Foutch AC, Rothenberg ML, Washington MK, Coffey RJ. TACE/ADAM-17: a component of the epidermal growth factor receptor axis and a promising therapeutic target in colorectal cancer. Clin. Cancer Res. 2008 Feb 2/15/2008; 14(4): 1182-91. PMID: 18281553, PMCID: PMC3718282, PII: 14/4/1182, DOI: 10.1158/1078-0432.CCR-07-1216, ISSN: 1078-0432.

Kim I, Li C, Liang D, Chen XZ, Coffey RJ, Ma J, Zhao P, Wu G. Polycystin-2 expression is regulated by a PC2-binding domain in the intracellular portion of fibrocystin. 2008.

Ding W, Li C, Hu T, Graves-Deal R, Fotia AB, Weissman AM and Coffey RJ. EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation. 2008.

Tahinci E, Thorne CA, Franklin JL, Salic A, Christian KM, Lee LA, Coffey RJ, Lee E. Lrp6 is required for convergent extension during Xenopus gastrulation. Development. 2007 Nov; 134(22): 4095-106. PMID: 17965054, PMCID: PMC4428168, PII: 134/22/4095, DOI: 10.1242/dev.010272, ISSN: 0950-1991.

Van Raay TJ, Coffey RJ, Solnica-Krezel L. Zebrafish Naked1 and Naked2 antagonize both canonical and non-canonical Wnt signaling. Dev. Biol [print-electronic]. 2007 Sep 9/15/2007; 309(2): 151-68. PMID: 17689523, PMCID: PMC2729589, PII: S0012-1606(07)00792-0, DOI: 10.1016/j.ydbio.2007.04.018, ISSN: 0012-1606.

Li C, Hao M, Cao Z, Ding W, Graves-Deal R, Hu J, Piston DW, Coffey RJ. Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha containing exocytic vesicles at the lower lateral membrane of polarized MDCK cells. Mol. Biol. Cell [print-electronic]. 2007 Aug; 18(8): 3081-93. PMID: 17553928, PMCID: PMC1949375, PII: E07-02-0172, DOI: 10.1091/mbc.E07-02-0172, ISSN: 1059-1524.

Li JQ, Xu BJ, Shakhtour B, Deane N, Merchant N, Heslin MJ, Washington K, Coffey RJ, Beauchamp RD, Shyr Y, Billheimer D. Variability of in situ proteomic profiling and implications for study design in colorectal tumors. Int. J. Oncol. 2007 Jul; 31(1): 103-11. PMID: 17549410, ISSN: 1019-6439.

Keller JW, Haigis KM, Franklin JL, Whitehead RH, Jacks T, Coffey RJ. Oncogenic K-RAS subverts the antiapoptotic role of N-RAS and alters modulation of the N-RAS:gelsolin complex. Oncogene [print-electronic]. 2007 May 5/10/2007; 26(21): 3051-9. PMID: 17130841, PII: 1210103, DOI: 10.1038/sj.onc.1210103, ISSN: 0950-9232.

Nam KT, Varro A, Coffey RJ, Goldenring JR. Potentiation of oxyntic atrophy-induced gastric metaplasia in amphiregulin-deficient mice. Gastroenterology [print-electronic]. 2007 May; 132(5): 1804-19. PMID: 17484876, PII: S0016-5085(07)00564-1, DOI: 10.1053/j.gastro.2007.03.040, ISSN: 0016-5085.

Deane NG, Manning HC, Foutch AC, Washington MK, Aronow BJ, Bornhop DJ, Coffey RJ. Targeted imaging of colonic tumors in smad3-/- mice discriminates cancer and inflammation. Mol. Cancer Res. 2007 Apr; 5(4): 341-9. PMID: 17426249, PII: 5/4/341, DOI: 10.1158/1541-7786.MCR-06-0225, ISSN: 1541-7786.

Keller JW, Franklin JL, Graves-Deal R, Friedman DB, Whitwell CW, Coffey RJ. Oncogenic KRAS provides a uniquely powerful and variable oncogenic contribution among RAS family members in the colonic epithelium. J. Cell. Physiol. 2007 Mar; 210(3): 740-9. PMID: 17133351, DOI: 10.1002/jcp.20898, ISSN: 0021-9541.

Coffey RJ, Washington MK, Corless CL, Heinrich MC. Ménétrier disease and gastrointestinal stromal tumors: hyperproliferative disorders of the stomach. J. Clin. Invest. 2007 Jan; 117(1): 70-80. PMID: 17200708, PMCID: PMC1716220, DOI: 10.1172/JCI30491, ISSN: 0021-9738.

Kaiser S, Park YK, Franklin JL, Halberg RB, Yu M, Jessen WJ, Freudenberg J, Chen X, Haigis K, Jegga AG, Kong S, Sakthivel B, Xu H, Reichling T, Azhar M, Boivin GP, Roberts RB, Bissahoyo AC, Gonzales F, Bloom GC, Eschrich S, Carter SL, Aronow JE, Kleimeyer J, Kleimeyer M, Ramaswamy V, Settle SH, Boone B, Levy S, Graff JM, Doetschman T, Groden J, Dove WF, Threadgill DW, Yeatman TJ, Coffey RJ, Aronow BJ. Transcriptional recapitulation and subversion of embryonic colon development by mouse colon tumor models and human colon cancer. Genome Biol. 2007; 8(7): R131. PMID: 17615082, PMCID: PMC2323222, PII: gb-2007-8-7-r131, DOI: 10.1186/gb-2007-8-7-r131, ISSN: 1474-760X.

Hu T, Krezel AM, Li C, Coffey RJ. Structural studies of human Naked2: a biologically active intrinsically unstructured protein. Biochem. Biophys. Res. Commun [print-electronic]. 2006 Dec 12/1/2006; 350(4): 911-5. PMID: 17045239, PMCID: PMC1661664, PII: S0006-291X(06)02166-8, DOI: 10.1016/j.bbrc.2006.09.121, ISSN: 0006-291X.

Johnson JC, Schmidt CR, Shrubsole MJ, Billheimer DD, Joshi PR, Morrow JD, Heslin MJ, Washington MK, Ness RM, Zheng W, Schwartz DA, Coffey RJ, Beauchamp RD, Merchant NB. Urine PGE-M: A metabolite of prostaglandin E2 as a potential biomarker of advanced colorectal neoplasia. Clin. Gastroenterol. Hepatol [print-electronic]. 2006 Nov; 4(11): 1358-65. PMID: 16996805, PII: S1542-3565(06)00772-5, DOI: 10.1016/j.cgh.2006.07.015, ISSN: 1542-3565.

Rothenberg ML, LaFleur B, Levy DE, Washington MK, Morgan-Meadows SL, Ramanathan RK, Berlin JD, Benson AB, Coffey RJ. Randomized phase II trial of the clinical and biological effects of two dose levels of gefitinib in patients with recurrent colorectal adenocarcinoma. J. Clin. Oncol. 2005 Dec 12/20/2005; 23(36): 9265-74. PMID: 16361624, PII: 23/36/9265, DOI: 10.1200/JCO.2005.03.0536, ISSN: 0732-183X.

Amann JM, Chyla BJ, Ellis TC, Martinez A, Moore AC, Franklin JL, McGhee L, Meyers S, Ohm JE, Luce KS, Ouelette AJ, Washington MK, Thompson MA, King D, Gautam S, Coffey RJ, Whitehead RH, Hiebert SW. Mtgr1 is a transcriptional corepressor that is required for maintenance of the secretory cell lineage in the small intestine. Mol. Cell. Biol. 2005 Nov; 25(21): 9576-85. PMID: 16227606, PMCID: PMC1265807, PII: 25/21/9576, DOI: 10.1128/MCB.25.21.9576-9585.2005, ISSN: 0270-7306.

Chung E, Graves-Deal R, Franklin JL, Coffey RJ. Differential effects of amphiregulin and TGF-alpha on the morphology of MDCK cells. Exp. Cell Res. 2005 Sep 9/10/2005; 309(1): 149-60. PMID: 15979068, PII: S0014-4827(05)00243-0, DOI: 10.1016/j.yexcr.2005.05.012, ISSN: 0014-4827.

Merchant NB, Rogers CM, Trivedi B, Morrow J, Coffey RJ. Ligand-dependent activation of the epidermal growth factor receptor by secondary bile acids in polarizing colon cancer cells. Surgery. 2005 Sep; 138(3): 415-21. PMID: 16213893, PII: S0039-6060(05)00364-8, DOI: 10.1016/j.surg.2005.06.030, ISSN: 0039-6060.

Means AL, Meszoely IM, Suzuki K, Miyamoto Y, Rustgi AK, Coffey RJ, Wright CV, Stoffers DA, Leach SD. Pancreatic epithelial plasticity mediated by acinar cell transdifferentiation and generation of nestin-positive intermediates. Development [print-electronic]. 2005 Aug; 132(16): 3767-76. PMID: 16020518, PII: dev.01925, DOI: 10.1242/dev.01925, ISSN: 0950-1991.

Schmidt CR, Gi YJ, Patel TA, Coffey RJ, Beauchamp RD, Pearson AS. E-cadherin is regulated by the transcriptional repressor SLUG during Ras-mediated transformation of intestinal epithelial cells. Surgery. 2005 Aug; 138(2): 306-12. PMID: 16153441, PII: S0039-6060(05)00277-1, DOI: 10.1016/j.surg.2005.06.007, ISSN: 0039-6060.

Settle SH, Washington K, Lind C, Itzkowitz S, Fiske WH, Burdick JS, Jerome WG, Ray M, Weinstein W, Coffey RJ. Chronic treatment of Ménétrier's disease with Erbitux: clinical efficacy and insight into pathophysiology. Clin. Gastroenterol. Hepatol. 2005 Jul; 3(7): 654-9. PMID: 16206497, ISSN: 1542-3565.

Braun AH, Coffey RJ. Lysophosphatidic acid, a disintegrin and metalloprotease-17 and heparin-binding epidermal growth factor-like growth factor in ovarian cancer: the first word, not the last. Clin. Cancer Res. 2005 Jul 7/1/2005; 11(13): 4639-43. PMID: 16000554, PII: 11/13/4639, DOI: 10.1158/1078-0432.CCR-05-0973, ISSN: 1078-0432.

Chung E, Cook PW, Parkos CA, Park YK, Pittelkow MR, Coffey RJ. Amphiregulin causes functional downregulation of adherens junctions in psoriasis. J. Invest. Dermatol. 2005 Jun; 124(6): 1134-40. PMID: 15955087, PII: JID23762, DOI: 10.1111/j.0022-202X.2005.23762.x, ISSN: 0022-202X.

Nomura S, Settle SH, Leys CM, Means AL, Peek RM, Leach SD, Wright CV, Coffey RJ, Goldenring JR. Evidence for repatterning of the gastric fundic epithelium associated with Ménétrier's disease and TGFalpha overexpression. Gastroenterology. 2005 May; 128(5): 1292-305. PMID: 15887112, PII: S0016508505004038, ISSN: 0016-5085.

Means AL, Chytil A, Moses HL, Coffey RJ, Wright CV, Taketo MM, Grady WM. Keratin 19 gene drives Cre recombinase expression throughout the early postimplantation mouse embryo. Genesis. 2005 May; 42(1): 23-7. PMID: 15828001, DOI: 10.1002/gene.20119, ISSN: 1526-954X.

Franklin JL, Yoshiura K, Dempsey PJ, Bogatcheva G, Jeyakumar L, Meise KS, Pearsall RS, Threadgill D, Coffey RJ. Identification of MAGI-3 as a transforming growth factor-alpha tail binding protein. Exp. Cell Res. 2005 Feb 2/15/2005; 303(2): 457-70. PMID: 15652357, PII: S0014-4827(04)00612-3, DOI: 10.1016/j.yexcr.2004.10.007, ISSN: 0014-4827.

Park YK, Franklin JL, Settle SH, Levy SE, Chung E, Jeyakumar LH, Shyr Y, Washington MK, Whitehead RH, Aronow BJ, Coffey RJ. Gene expression profile analysis of mouse colon embryonic development. Genesis. 2005 Jan; 41(1): 1-12. PMID: 15645444, DOI: 10.1002/gene.20088, ISSN: 1526-954X.

Schmidt CR, Gi YJ, Coffey RJ, Beauchamp RD, Pearson AS. Oncogenic Ras dominates overexpression of E-cadherin in malignant transformation of intestinal epithelial cells. Surgery. 2004 Aug; 136(2): 303-9. PMID: 15300195, PII: S0039606004002442, DOI: 10.1016/j.surg.2004.05.004, ISSN: 0039-6060.

Organ EL, Nalbantyan CD, Nanney LB, Woodward SC, Sheng J, Dubois RN, Price J, Sutcliffe M, Coffey RJ, Rubin DH. Effects of transforming growth factor-alpha (TGF-alpha) in vitro and in vivo on reovirus replication. DNA Cell Biol. 2004 Jul; 23(7): 430-41. PMID: 15294092, DOI: 10.1089/1044549041474751, ISSN: 1044-5498.

Murphy SJ, Doré JJ, Edens M, Coffey RJ, Barnard JA, Mitchell H, Wilkes M, Leof EB. Differential trafficking of transforming growth factor-beta receptors and ligand in polarized epithelial cells. Mol. Biol. Cell [print-electronic]. 2004 Jun; 15(6): 2853-62. PMID: 15075369, PMCID: PMC420108, PII: E04-02-0097, DOI: 10.1091/mbc.E04-02-0097, ISSN: 1059-1524.

Li C, Franklin JL, Graves-Deal R, Jerome WG, Cao Z, Coffey RJ. Myristoylated Naked2 escorts transforming growth factor alpha to the basolateral plasma membrane of polarized epithelial cells. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2004 Apr 4/13/2004; 101(15): 5571-6. PMID: 15064403, PMCID: PMC397424, PII: 0401294101, DOI: 10.1073/pnas.0401294101, ISSN: 0027-8424.

Du J, Jiang B, Coffey RJ, Barnard J. Raf and RhoA cooperate to transform intestinal epithelial cells and induce growth resistance to transforming growth factor beta. Mol. Cancer Res. 2004 Apr; 2(4): 233-41. PMID: 15140945, ISSN: 1541-7786.

Kuwada SK, Scaife CL, Kuang J, Li X, Wong RF, Florell SR, Coffey RJ, Gray PD. Effects of trastuzumab on epidermal growth factor receptor-dependent and -independent human colon cancer cells. Int. J. Cancer. 2004 Mar 3/20/2004; 109(2): 291-301. PMID: 14750183, DOI: 10.1002/ijc.11686, ISSN: 0020-7136.

Friedman DB, Hill S, Keller JW, Merchant NB, Levy SE, Coffey RJ, Caprioli RM. Proteome analysis of human colon cancer by two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics. 2004 Mar; 4(3): 793-811. PMID: 14997500, DOI: 10.1002/pmic.200300635, ISSN: 1615-9853.

Ohashi M, Sakurai M, Higuchi M, Mori N, Fukushi M, Oie M, Coffey RJ, Yoshiura K, Tanaka Y, Uchiyama M, Hatanaka M, Fujii M. Human T-cell leukemia virus type 1 Tax oncoprotein induces and interacts with a multi-PDZ domain protein, MAGI-3. Virology. 2004 Mar 3/1/2004; 320(1): 52-62. PMID: 15003862, PII: S0042682203008444, DOI: 10.1016/j.virol.2003.11.014, ISSN: 0042-6822.

Zhang MZ, Mai W, Li C, Cho SY, Hao C, Moeckel G, Zhao R, Kim I, Wang J, Xiong H, Wang H, Sato Y, Wu Y, Nakanuma Y, Lilova M, Pei Y, Harris RC, Li S, Coffey RJ, Sun L, Wu D, Chen XZ, Breyer MD, Zhao ZJ, McKanna JA, Wu G. PKHD1 protein encoded by the gene for autosomal recessive polycystic kidney disease associates with basal bodies and primary cilia in renal epithelial cells. Proc. Natl. Acad. Sci. U.S.A. 2004 Feb 2/24/2004; 101(8): 2311-6. PMID: 14983006, PMCID: PMC356947, PII: 101/8/2311, ISSN: 0027-8424.

Schmidt CR, Washington MK, Gi YJ, Coffey RJ, Beauchamp RD, Pearson AS. Dysregulation of E-cadherin by oncogenic Ras in intestinal epithelial cells is blocked by inhibiting MAP kinase. Am. J. Surg. 2003 Nov; 186(5): 426-30. PMID: 14599601, PII: S0002961003003271, ISSN: 0002-9610.

Dempsey PJ, Meise KS, Coffey RJ. Basolateral sorting of transforming growth factor-alpha precursor in polarized epithelial cells: characterization of cytoplasmic domain determinants. Exp. Cell Res. 2003 May 5/1/2003; 285(2): 159-74. PMID: 12706112, PII: S0014482703000351, ISSN: 0014-4827.

Cutler NS, Graves-Deal R, LaFleur BJ, Gao Z, Boman BM, Whitehead RH, Terry E, Morrow JD, Coffey RJ. Stromal production of prostacyclin confers an antiapoptotic effect to colonic epithelial cells. Cancer Res. 2003 Apr 4/15/2003; 63(8): 1748-51. PMID: 12702555, ISSN: 0008-5472.

Means AL, Ray KC, Singh AB, Washington MK, Whitehead RH, Harris RC, Wright CV, Coffey RJ, Leach SD. Overexpression of heparin-binding EGF-like growth factor in mouse pancreas results in fibrosis and epithelial metaplasia. Gastroenterology. 2003 Apr; 124(4): 1020-36. PMID: 12671899, PII: S0016508503000647, DOI: 10.1053/gast.2003.50150, ISSN: 0016-5085.

Harris RC, Chung E, Coffey RJ. EGF receptor ligands. Exp. Cell Res. 2003 Mar 3/10/2003; 284(1): 2-13. PMID: 12648462, PII: S0014482702001052, ISSN: 0014-4827.

Boivin GP, Washington K, Yang K, Ward JM, Pretlow TP, Russell R, Besselsen DG, Godfrey VL, Doetschman T, Dove WF, Pitot HC, Halberg RB, Itzkowitz SH, Groden J, Coffey RJ. Pathology of mouse models of intestinal cancer: consensus report and recommendations. Gastroenterology. 2003 Mar; 124(3): 762-77. PMID: 12612914, PII: S0016508502159725, DOI: 10.1053/gast.2003.50094, ISSN: 0016-5085.

McCole DF, Keely SJ, Coffey RJ, Barrett KE. Transactivation of the epidermal growth factor receptor in colonic epithelial cells by carbachol requires extracellular release of transforming growth factor-alpha. J. Biol. Chem [print-electronic]. 2002 Nov 11/8/2002; 277(45): 42603-12. PMID: 12202486, PII: M206487200, DOI: 10.1074/jbc.M206487200, ISSN: 0021-9258.

Cox B, Murphey LJ, Zackert WE, Chinery R, Graves-Deal R, Boutaud O, Oates JA, Coffey RJ, Morrow JD. Human colorectal cancer cells efficiently conjugate the cyclopentenone prostaglandin, prostaglandin J(2), to glutathione. Biochim. Biophys. Acta. 2002 Sep 9/5/2002; 1584(1): 37-45. PMID: 12213491, PII: S1388198102002676, ISSN: 0006-3002.

Roberts RB, Min L, Washington MK, Olsen SJ, Settle SH, Coffey RJ, Threadgill DW. Importance of epidermal growth factor receptor signaling in establishment of adenomas and maintenance of carcinomas during intestinal tumorigenesis. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2002 Feb 2/5/2002; 99(3): 1521-6. PMID: 11818567, PMCID: PMC122223, PII: 032678499, DOI: 10.1073/pnas.032678499, ISSN: 0027-8424.

Brown CL, Coffey RJ, Dempsey PJ. The proamphiregulin cytoplasmic domain is required for basolateral sorting, but is not essential for constitutive or stimulus-induced processing in polarized Madin-Darby canine kidney cells. J. Biol. Chem [print-electronic]. 2001 Aug 8/3/2001; 276(31): 29538-49. PMID: 11382759, PII: M102114200, DOI: 10.1074/jbc.M102114200, ISSN: 0021-9258.

Pianetti S, Arsura M, Romieu-Mourez R, Coffey RJ, Sonenshein GE. Her-2/neu overexpression induces NF-kappaB via a PI3-kinase/Akt pathway involving calpain-mediated degradation of IkappaB-alpha that can be inhibited by the tumor suppressor PTEN. Oncogene. 2001 Mar 3/15/2001; 20(11): 1287-99. PMID: 11313873, DOI: 10.1038/sj.onc.1204257, ISSN: 0950-9232.

Neuzil J, Weber T, Schröder A, Lu M, Ostermann G, Gellert N, Mayne GC, Olejnicka B, Nègre-Salvayre A, Stícha M, Coffey RJ, Weber C. Induction of cancer cell apoptosis by alpha-tocopheryl succinate: molecular pathways and structural requirements. FASEB J. 2001 Feb; 15(2): 403-15. PMID: 11156956, PII: 15/2/403, DOI: 10.1096/fj.00-0251com, ISSN: 0892-6638.

Blanke CD, Stipanov M, Morrow J, Rothenberg M, Chinery R, Shyr Y, Coffey R, Johnson DH, Leach SD, Beauchamp RD. A phase I study of vitamin E, 5-fluorouracil and leucovorin for advanced malignancies. 2001.

Burdick JS, Chung E, Tanner G, Sun M, Paciga JE, Cheng JQ, Washington K, Goldenring JR, Coffey RJ. Treatment of Ménétrier's disease with a monoclonal antibody against the epidermal growth factor receptor. N. Engl. J. Med. 2000 Dec 12/7/2000; 343(23): 1697-701. PMID: 11106719, DOI: 10.1056/NEJM200012073432305, ISSN: 0028-4793.

Lee MP, Ravenel JD, Hu RJ, Lustig LR, Tomaselli G, Berger RD, Brandenburg SA, Litzi TJ, Bunton TE, Limb C, Francis H, Gorelikow M, Gu H, Washington K, Argani P, Goldenring JR, Coffey RJ, Feinberg AP. Targeted disruption of the Kvlqt1 gene causes deafness and gastric hyperplasia in mice. J. Clin. Invest. 2000 Dec; 106(12): 1447-55. PMID: 11120752, PMCID: PMC387258, DOI: 10.1172/JCI10897, ISSN: 0021-9738.

Lenferink AE, Simpson JF, Shawver LK, Coffey RJ, Forbes JT, Arteaga CL. Blockade of the epidermal growth factor receptor tyrosine kinase suppresses tumorigenesis in MMTV/Neu + MMTV/TGF-alpha bigenic mice. Proc. Natl. Acad. Sci. U.S.A. 2000 Aug 8/15/2000; 97(17): 9609-14. PMID: 10931950, PMCID: PMC16912, PII: 160564197, DOI: 10.1073/pnas.160564197, ISSN: 0027-8424.

Bulus NM, Sheng HM, Sizemore N, Oldham SM, Barnett JV, Coffey RJ, Beauchamp DR, Barnard JA. Ras-mediated suppression of TGFbetaRII expression in intestinal epithelial cells involves Raf-independent signaling. Neoplasia. 2000 Jul; 2(4): 357-64. PMID: 11005570, PMCID: PMC1550294, ISSN: 1522-8002.

Denning MF, Dlugosz AA, Cheng C, Dempsey PJ, Coffey RJ, Threadgill DW, Magnuson T, Yuspa SH. Cross-talk between epidermal growth factor receptor and protein kinase C during calcium-induced differentiation of keratinocytes. Exp. Dermatol. 2000 Jun; 9(3): 192-9. PMID: 10839717, ISSN: 0906-6705.

Goldenring JR, Ray GS, Coffey RJ, Meunier PC, Haley PJ, Barnes TB, Car BD. Reversible drug-induced oxyntic atrophy in rats. Gastroenterology. 2000 Jun; 118(6): 1080-93. PMID: 10833483, PII: S0016508500251962, ISSN: 0016-5085.

Song SY, Meszoely IM, Coffey RJ, Pietenpol JA, Leach SD. K-Ras-independent effects of the farnesyl transferase inhibitor L-744,832 on cyclin B1/Cdc2 kinase activity, G2/M cell cycle progression and apoptosis in human pancreatic ductal adenocarcinoma cells. Neoplasia. 2000 May; 2(3): 261-72. PMID: 10935512, PMCID: PMC1507570, ISSN: 1522-8002.

Donaldson JC, Dempsey PJ, Reddy S, Bouton AH, Coffey RJ, Hanks SK. Crk-associated substrate p130(Cas) interacts with nephrocystin and both proteins localize to cell-cell contacts of polarized epithelial cells. Exp. Cell Res. 2000 Apr 4/10/2000; 256(1): 168-78. PMID: 10739664, PII: S0014-4827(00)94822-5, DOI: 10.1006/excr.2000.4822, ISSN: 0014-4827.

Bach SP, Chinery R, O'Dwyer ST, Potten CS, Coffey RJ, Watson AJ. Pyrrolidinedithiocarbamate increases the therapeutic index of 5-fluorouracil in a mouse model. Gastroenterology. 2000 Jan; 118(1): 81-9. PMID: 10611156, PII: S0016508500145214, ISSN: 0016-5085.

Wang TC, Dangler CA, Chen D, Goldenring JR, Koh T, Raychowdhury R, Coffey RJ, Ito S, Varro A, Dockray GJ, Fox JG. Synergistic interaction between hypergastrinemia and Helicobacter infection in a mouse model of gastric cancer. Gastroenterology. 2000 Jan; 118(1): 36-47. PMID: 10611152, PII: S0016508500790391, ISSN: 0016-5085.

Coffey RJ, Threadgill D. Microarray foray [editorial]. Breast Cancer Res [print-electronic]. 2000; 2(1): 8-9. PMID: 11250685, PMCID: PMC521206, ISSN: 1465-5411.

Song SY, Gannon M, Washington MK, Scoggins CR, Meszoely IM, Goldenring JR, Marino CR, Sandgren EP, Coffey RJ, Wright CV, Leach SD. Expansion of Pdx1-expressing pancreatic epithelium and islet neogenesis in transgenic mice overexpressing transforming growth factor alpha. Gastroenterology. 1999 Dec; 117(6): 1416-26. PMID: 10579983, PII: S0016508599005648, ISSN: 0016-5085.

Sizemore N, Cox AD, Barnard JA, Oldham SM, Reynolds ER, Der CJ, Coffey RJ. Pharmacological inhibition of Ras-transformed epithelial cell growth is linked to down-regulation of epidermal growth factor-related peptides. Gastroenterology. 1999 Sep; 117(3): 567-76. PMID: 10464132, PII: S0016508599002413, ISSN: 0016-5085.

Damstrup L, Kuwada SK, Dempsey PJ, Brown CL, Hawkey CJ, Poulsen HS, Wiley HS, Coffey RJ. Amphiregulin acts as an autocrine growth factor in two human polarizing colon cancer lines that exhibit domain selective EGF receptor mitogenesis. Br. J. Cancer. 1999 Jun; 80(7): 1012-9. PMID: 10362109, PMCID: PMC2363033, DOI: 10.1038/sj.bjc.6690456, ISSN: 0007-0920.

Chinery R, Coffey RJ, Graves-Deal R, Kirkland SC, Sanchez SC, Zackert WE, Oates JA, Morrow JD. Prostaglandin J2 and 15-deoxy-delta12,14-prostaglandin J2 induce proliferation of cyclooxygenase-depleted colorectal cancer cells. Cancer Res. 1999 Jun 6/1/1999; 59(11): 2739-46. PMID: 10364000, ISSN: 0008-5472.

Dong J, Opresko LK, Dempsey PJ, Lauffenburger DA, Coffey RJ, Wiley HS. Metalloprotease-mediated ligand release regulates autocrine signaling through the epidermal growth factor receptor. Proc. Natl. Acad. Sci. U.S.A. 1999 May 5/25/1999; 96(11): 6235-40. PMID: 10339571, PMCID: PMC26865, ISSN: 0027-8424.

Lee HM, Udupi V, Englander EW, Rajaraman S, Coffey RJ, Jr., Greeley GH, Jr.. Stimulatory actions of insulin-like growth factor-I and transforming growth factor-alpha on intestinal neurotensin and peptide YY. 1999.

Nørgaard P, Law B, Joseph H, Page DL, Shyr Y, Mays D, Pietenpol JA, Kohl NE, Oliff A, Coffey RJ, Poulsen HS, Moses HL. Treatment with farnesyl-protein transferase inhibitor induces regression of mammary tumors in transforming growth factor (TGF) alpha and TGF alpha/neu transgenic mice by inhibition of mitogenic activity and induction of apoptosis. Clin. Cancer Res. 1999 Jan; 5(1): 35-42. PMID: 9918200, ISSN: 1078-0432.

Kuwada SK, Li XF, Damstrup L, Dempsey PJ, Coffey RJ, Wiley HS. The dynamic expression of the epidermal growth factor receptor and epidermal growth factor ligand family in a differentiating intestinal epithelial cell line. Growth Factors. 1999; 17(2): 139-53. PMID: 10595313, ISSN: 0897-7194.

Auer KL, Park JS, Seth P, Coffey RJ, Darlington G, Abo A, McMahon M, Depinho RA, Fisher PB, Dent P. Prolonged activation of the mitogen-activated protein kinase pathway promotes DNA synthesis in primary hepatocytes from p21Cip-1/WAF1-null mice, but not in hepatocytes from p16INK4a-null mice. Biochem. J. 1998 Dec 12/15/1998; 336 ( Pt 3): 551-60. PMID: 9841865, PMCID: PMC1219904, ISSN: 0264-6021.

DiGiovanna MP, Lerman MA, Coffey RJ, Muller WJ, Cardiff RD, Stern DF. Active signaling by Neu in transgenic mice. Oncogene. 1998 Oct 10/8/1998; 17(14): 1877-84. PMID: 9778054, DOI: 10.1038/sj.onc.1202091, ISSN: 0950-9232.

Zhang R, Averboukh L, Zhu W, Zhang H, Jo H, Dempsey PJ, Coffey RJ, Pardee AB, Liang P. Identification of rCop-1, a new member of the CCN protein family, as a negative regulator for cell transformation. Mol. Cell. Biol. 1998 Oct; 18(10): 6131-41. PMID: 9742130, PMCID: PMC109199, ISSN: 0270-7306.

Brown CL, Meise KS, Plowman GD, Coffey RJ, Dempsey PJ. Cell surface ectodomain cleavage of human amphiregulin precursor is sensitive to a metalloprotease inhibitor. Release of a predominant N-glycosylated 43-kDa soluble form. J. Biol. Chem. 1998 Jul 7/3/1998; 273(27): 17258-68. PMID: 9642297, ISSN: 0021-9258.

Acra SA, Bulus N, Bogatcheva G, Coffey RJ, Barnard JA. Increased intestinal epithelial proliferation in metallothioneine-transforming growth factor alpha transgenic mice. Regul. Pept. 1998 Jun 6/30/1998; 74(2-3): 105-12. PMID: 9712170, PII: S0167-0115(98)00029-9, ISSN: 0167-0115.

Chinery R, Beauchamp RD, Shyr Y, Kirkland SC, Coffey RJ, Morrow JD. Antioxidants reduce cyclooxygenase-2 expression, prostaglandin production, and proliferation in colorectal cancer cells. Cancer Res. 1998 Jun 6/1/1998; 58(11): 2323-7. PMID: 9622066, ISSN: 0008-5472.

Oldham SM, Cox AD, Reynolds ER, Sizemore NS, Coffey RJ, Der CJ. Ras, but not Src, transformation of RIE-1 epithelial cells is dependent on activation of the mitogen-activated protein kinase cascade. Oncogene. 1998 May; 16(20): 2565-73. PMID: 9632133, DOI: 10.1038/sj.onc.1201784, ISSN: 0950-9232.

Romano M, Ricci V, Di Popolo A, Sommi P, Del Vecchio Blanco C, Bruni CB, Ventura U, Cover TL, Blaser MJ, Coffey RJ, Zarrilli R. Helicobacter pylori upregulates expression of epidermal growth factor-related peptides, but inhibits their proliferative effect in MKN 28 gastric mucosal cells. J. Clin. Invest. 1998 Apr 4/15/1998; 101(8): 1604-13. PMID: 9541490, PMCID: PMC508741, DOI: 10.1172/JCI1174, ISSN: 0021-9738.

Sheng GG, Shao J, Sheng H, Hooton EB, Isakson PC, Morrow JD, Coffey RJ, DuBois RN, Beauchamp RD. A selective cyclooxygenase 2 inhibitor suppresses the growth of H-ras-transformed rat intestinal epithelial cells. Gastroenterology. 1997 Dec; 113(6): 1883-91. PMID: 9394727, PII: S0016508597005556, ISSN: 0016-5085.

Chinery R, Brockman JA, Dransfield DT, Coffey RJ. Antioxidant-induced nuclear translocation of CCAAT/enhancer-binding protein beta. A critical role for protein kinase A-mediated phosphorylation of Ser299. J. Biol. Chem. 1997 Nov 11/28/1997; 272(48): 30356-61. PMID: 9374525, ISSN: 0021-9258.

Chinery R, Brockman JA, Peeler MO, Shyr Y, Beauchamp RD, Coffey RJ. Antioxidants enhance the cytotoxicity of chemotherapeutic agents in colorectal cancer: a p53-independent induction of p21WAF1/CIP1 via C/EBPbeta. Nat. Med. 1997 Nov; 3(11): 1233-41. PMID: 9359698, ISSN: 1078-8956.

Dempsey PJ, Meise KS, Yoshitake Y, Nishikawa K, Coffey RJ. Apical enrichment of human EGF precursor in Madin-Darby canine kidney cells involves preferential basolateral ectodomain cleavage sensitive to a metalloprotease inhibitor. J. Cell Biol. 1997 Aug 8/25/1997; 138(4): 747-58. PMID: 9265643, PMCID: PMC2138042, ISSN: 0021-9525.

Dlugosz AA, Hansen L, Cheng C, Alexander N, Denning MF, Threadgill DW, Magnuson T, Coffey RJ, Yuspa SH. Targeted disruption of the epidermal growth factor receptor impairs growth of squamous papillomas expressing the v-ras(Ha) oncogene but does not block in vitro keratinocyte responses to oncogenic ras. Cancer Res. 1997 Aug 8/1/1997; 57(15): 3180-8. PMID: 9242447, ISSN: 0008-5472.

Gangarosa LM, Sizemore N, Graves-Deal R, Oldham SM, Der CJ, Coffey RJ. A raf-independent epidermal growth factor receptor autocrine loop is necessary for Ras transformation of rat intestinal epithelial cells. J. Biol. Chem. 1997 Jul 7/25/1997; 272(30): 18926-31. PMID: 9228072, ISSN: 0021-9258.

Sheng H, Shao J, Kirkland SC, Isakson P, Coffey RJ, Morrow J, Beauchamp RD, DuBois RN. Inhibition of human colon cancer cell growth by selective inhibition of cyclooxygenase-2. J. Clin. Invest. 1997 May 5/1/1997; 99(9): 2254-9. PMID: 9151799, PMCID: PMC508057, DOI: 10.1172/JCI119400, ISSN: 0021-9738.

Zhang R, Zhang H, Zhu W, Pardee AB, Coffey RJ, Liang P. Mob-1, a Ras target gene, is overexpressed in colorectal cancer. Oncogene. 1997 Apr 4/3/1997; 14(13): 1607-10. PMID: 9129152, DOI: 10.1038/sj.onc.1200957, ISSN: 0950-9232.

Scheiman JM, Meise KS, Greenson JK, Coffey RJ. Transforming growth factor-alpha (TGF-alpha) levels in human proximal gastrointestinal epithelium. Effect of mucosal injury and acid inhibition. Dig. Dis. Sci. 1997 Feb; 42(2): 333-41. PMID: 9052515, ISSN: 0163-2116.

Coffey RJ, Hawkey CJ, Damstrup L, Graves-Deal R, Daniel VC, Dempsey PJ, Chinery R, Kirkland SC, DuBois RN, Jetton TL, Morrow JD. Epidermal growth factor receptor activation induces nuclear targeting of cyclooxygenase-2, basolateral release of prostaglandins, and mitogenesis in polarizing colon cancer cells. Proc. Natl. Acad. Sci. U.S.A. 1997 Jan 1/21/1997; 94(2): 657-62. PMID: 9012840, PMCID: PMC19569, ISSN: 0027-8424.

Deckard-Janatpour K, Muller WJ, Chodosh L, Gardner H, Marquis S, Coffey R, Cardiff RD. Differential expression of the neu transgene in murine mammary tissues. 1997.

Rage F, Hill DF, Sena-Esteves M, Breakefield XO, Coffey RJ, Costa ME, McCann SM, Ojeda SR. Targeting transforming growth factor alpha expression to discrete loci of the neuroendocrine brain induces female sexual precocity. 1997.

Chinery R, Coffey RJ. Trefoil peptides: less clandestine in the intestine. Science. 1996 Oct 10/11/1996; 274(5285): 204. PMID: 8927980, ISSN: 0036-8075.

Muller WJ, Arteaga CL, Muthuswamy SK, Siegel PM, Webster MA, Cardiff RD, Meise KS, Li F, Halter SA, Coffey RJ. Synergistic interaction of the Neu proto-oncogene product and transforming growth factor alpha in the mammary epithelium of transgenic mice. Mol. Cell. Biol. 1996 Oct; 16(10): 5726-36. PMID: 8816486, PMCID: PMC231573, ISSN: 0270-7306.

Oldham SM, Clark GJ, Gangarosa LM, Coffey RJ, Der CJ. Activation of the Raf-1/MAP kinase cascade is not sufficient for Ras transformation of RIE-1 epithelial cells. Proc. Natl. Acad. Sci. U.S.A. 1996 Jul 7/9/1996; 93(14): 6924-8. PMID: 8692920, PMCID: PMC38910, ISSN: 0027-8424.

Romano M, Lesch CA, Meise KS, Veljaca M, Sanchez B, Kraus ER, Boland CR, Guglietta A, Coffey RJ. Increased gastroduodenal concentrations of transforming growth factor alpha in adaptation to aspirin in monkeys and rats. Gastroenterology. 1996 May; 110(5): 1448-55. PMID: 8613050, PII: S0016508596002168, ISSN: 0016-5085.

Goldenring JR, Ray GS, Soroka CJ, Smith J, Modlin IM, Meise KS, Coffey RJ. Overexpression of transforming growth factor-alpha alters differentiation of gastric cell lineages. Dig. Dis. Sci. 1996 Apr; 41(4): 773-84. PMID: 8674399, ISSN: 0163-2116.

Rutten MJ, Dempsey PJ, Luttropp CA, Hawkey MA, Sheppard BC, Crass RA, Deveney CW, Coffey RJ. Identification of an EGF/TGF-alpha receptor in primary cultures of guinea pig gastric mucous epithelial cells. Am. J. Physiol. 1996 Apr; 270(4 Pt 1): G604-12. PMID: 8928790, ISSN: 0002-9513.

Gangarosa LM, Dempsey PJ, Damstrup L, Barnard JA, Coffey RJ. Transforming growth factor-alpha. Baillieres Clin. Gastroenterol. 1996 Mar; 10(1): 49-63. PMID: 8732300, ISSN: 0950-3528.

Goldenring JR, Poulsom R, Ray GS, Wright N, Meise KS, Coffey RJ. Expression of trefoil peptides in the gastric mucosa of transgenic mice overexpressing transforming growth factor-alpha. Growth Factors. 1996; 13(1-2): 111-9. PMID: 8962716, ISSN: 0897-7194.

Bluth RF, Carpenter HA, Pittelkow MR, Page DL, Coffey RJ. Immunolocalization of transforming growth factor-alpha in normal and diseased human gastric mucosa. Hum. Pathol. 1995 Dec; 26(12): 1333-40. PMID: 8522306, ISSN: 0046-8177.

Coffey RJ, Gangarosa LM, Damstrup L, Dempsey PJ. Basic actions of transforming growth factor-alpha and related peptides. Eur J Gastroenterol Hepatol. 1995 Oct; 7(10): 923-7. PMID: 8590135, ISSN: 0954-691X.

Homma T, Sakai M, Cheng HF, Yasuda T, Coffey RJ, Harris RC. Induction of heparin-binding epidermal growth factor-like growth factor mRNA in rat kidney after acute injury. J. Clin. Invest. 1995 Aug; 96(2): 1018-25. PMID: 7635938, PMCID: PMC185290, DOI: 10.1172/JCI118087, ISSN: 0021-9738.

Pierce DF, Gorska AE, Chytil A, Meise KS, Page DL, Coffey RJ, Moses HL. Mammary tumor suppression by transforming growth factor beta 1 transgene expression. Proc. Natl. Acad. Sci. U.S.A. 1995 May 5/9/1995; 92(10): 4254-8. PMID: 7753792, PMCID: PMC41922, ISSN: 0027-8424.

DuBois RN, Bishop PR, Graves-Deal R, Coffey RJ. Transforming growth factor alpha regulation of two zinc finger-containing immediate early response genes in intestine. Cell Growth Differ. 1995 May; 6(5): 523-9. PMID: 7647035, ISSN: 1044-9523.

Lin CQ, Dempsey PJ, Coffey RJ, Bissell MJ. Extracellular matrix regulates whey acidic protein gene expression by suppression of TGF-alpha in mouse mammary epithelial cells: studies in culture and in transgenic mice. J. Cell Biol. 1995 May; 129(4): 1115-26. PMID: 7744960, PMCID: PMC2120484, ISSN: 0021-9525.

Dlugosz AA, Cheng C, Williams EK, Darwiche N, Dempsey PJ, Mann B, Dunn AR, Coffey RJ, Yuspa SH. Autocrine transforming growth factor alpha is dispensible for v-rasHa-induced epidermal neoplasia: potential involvement of alternate epidermal growth factor receptor ligands. Cancer Res. 1995 May 5/1/1995; 55(9): 1883-93. PMID: 7728756, ISSN: 0008-5472.

Witty JP, Lempka T, Coffey RJ, Matrisian LM. Decreased tumor formation in 7,12-dimethylbenzanthracene-treated stromelysin-1 transgenic mice is associated with alterations in mammary epithelial cell apoptosis. Cancer Res. 1995 Apr 4/1/1995; 55(7): 1401-6. PMID: 7882342, ISSN: 0008-5472.

Barnard JA, Beauchamp RD, Russell WE, Dubois RN, Coffey RJ. Epidermal growth factor-related peptides and their relevance to gastrointestinal pathophysiology. Gastroenterology. 1995 Feb; 108(2): 564-80. PMID: 7835600, ISSN: 0016-5085.

Romano M, Meise KS, Suozzo R, Sessa G, Persico M, Coffey RJ. Regional distribution of transforming growth factor-alpha and epidermal growth factor in normal and portal hypertensive gastric mucosa in humans. Dig. Dis. Sci. 1995 Feb; 40(2): 263-7. PMID: 7851187, ISSN: 0163-2116.

Threadgill DW, Dlugosz AA, Hansen LA, Tennenbaum T, Lichti U, Yee D, LaMantia C, Mourton T, Herrup K, Harris RC, Barnard JA, Yuspa SH, Coffey RJ, Magnuson T. Targeted disruption of mouse EGF receptor: effect of genetic background on mutant phenotype. 1995.

Coffey RJ, Romano M, Goldenring J. Roles for transforming growth factor-alpha in the stomach. J. Clin. Gastroenterol. 1995; 21 Suppl 1: S36-9. PMID: 8774988, ISSN: 0192-0790.

Dlugosz AA, Cheng C, Denning MF, Dempsey PJ, Coffey RJ, Yuspa SH. Keratinocyte growth factor receptor ligands induce transforming growth factor alpha expression and activate the epidermal growth factor receptor signaling pathway in cultured epidermal keratinocytes. Cell Growth Differ. 1994 Dec; 5(12): 1283-92. PMID: 7535082, ISSN: 1044-9523.

Reddy KB, Yee D, Hilsenbeck SG, Coffey RJ, Osborne CK. Inhibition of estrogen-induced breast cancer cell proliferation by reduction in autocrine transforming growth factor alpha expression. Cell Growth Differ. 1994 Dec; 5(12): 1275-82. PMID: 7696176, ISSN: 1044-9523.

Nanney LB, Coffey RJ, Ellis DL. Expression and distribution of transforming growth factor-alpha within melanocytic lesions. J. Invest. Dermatol. 1994 Nov; 103(5): 707-14. PMID: 7963661, ISSN: 0022-202X.

Eberhart CE, Coffey RJ, Radhika A, Giardiello FM, Ferrenbach S, DuBois RN. Up-regulation of cyclooxygenase 2 gene expression in human colorectal adenomas and adenocarcinomas. Gastroenterology. 1994 Oct; 107(4): 1183-8. PMID: 7926468, PII: S0016508594003033, ISSN: 0016-5085.

Barnard JA, Graves-Deal R, Pittelkow MR, DuBois R, Cook P, Ramsey GW, Bishop PR, Damstrup L, Coffey RJ. Auto- and cross-induction within the mammalian epidermal growth factor-related peptide family. J. Biol. Chem. 1994 Sep 9/9/1994; 269(36): 22817-22. PMID: 8077234, ISSN: 0021-9258.

Dempsey PJ, Coffey RJ. Basolateral targeting and efficient consumption of transforming growth factor-alpha when expressed in Madin-Darby canine kidney cells. J. Biol. Chem. 1994 Jun 6/17/1994; 269(24): 16878-89. PMID: 8207010, ISSN: 0021-9258.

Romano M, Kraus ER, Boland CR, Coffey RJ. Comparison between transforming growth factor alpha and epidermal growth factor in the protection of rat gastric mucosa against drug-induced injury. Ital J Gastroenterol. 1994 Jun; 26(5): 223-8. PMID: 7522648, ISSN: 0392-0623.

Coffey RJ, Meise KS, Matsui Y, Hogan BL, Dempsey PJ, Halter SA. Acceleration of mammary neoplasia in transforming growth factor alpha transgenic mice by 7,12-dimethylbenzanthracene. Cancer Res. 1994 Apr 4/1/1994; 54(7): 1678-83. PMID: 8137281, ISSN: 0008-5472.

Sakai S, Mizuno M, Harigaya T, Yamamoto K, Mori T, Coffey RJ, Nagasawa H. Cause of failure of lactation in mouse mammary tumor virus/human transforming growth factor alpha transgenic mice. Proc. Soc. Exp. Biol. Med. 1994 Mar; 205(3): 236-42. PMID: 8171044, ISSN: 0037-9727.

Pierce DF, Coffey RJ. Therapeutic manipulation of cytokines: transforming growth factor beta-1 protects mice treated with lethal doses of cytarabine. Am Surg. 1994 Jan; 60(1): 18-25. PMID: 8273969, ISSN: 0003-1348.

Guglietta A, Lesch CA, Romano M, McClure RW, Coffey RJ. Effect of transforming growth factor-alpha on gastric acid secretion in rats and monkeys. Dig. Dis. Sci. 1994 Jan; 39(1): 177-82. PMID: 8281854, ISSN: 0163-2116.

Russell WE, Dempsey PJ, Sitaric S, Peck AJ, Coffey RJ. Transforming growth factor-alpha (TGF alpha) concentrations increase in regenerating rat liver: evidence for a delayed accumulation of mature TGF alpha. Endocrinology. 1993 Oct; 133(4): 1731-8. PMID: 8404616, DOI: 10.1210/endo.133.4.8404616, ISSN: 0013-7227.

Rutten MJ, Dempsey PJ, Solomon TE, Coffey RJ. TGF-alpha is a potent mitogen for primary cultures of guinea pig gastric mucous epithelial cells. Am. J. Physiol. 1993 Aug; 265(2 Pt 1): G361-9. PMID: 8368318, ISSN: 0002-9513.

Chuncharunee S, Carter CD, Studtmann KE, Caro J, Coffey RJ, Dessypris EN. Chronic administration of transforming growth factor-beta suppresses erythropoietin-dependent erythropoiesis and induces tumour necrosis factor in vivo. Br. J. Haematol. 1993 Jul; 84(3): 374-80. PMID: 8217788, ISSN: 0007-1048.

Pittelkow MR, Cook PW, Shipley GD, Derynck R, Coffey RJ. Autonomous growth of human keratinocytes requires epidermal growth factor receptor occupancy. Cell Growth Differ. 1993 Jun; 4(6): 513-21. PMID: 8373735, ISSN: 1044-9523.

Cheng C, Tennenbaum T, Dempsey PJ, Coffey RJ, Yuspa SH, Dlugosz AA. Epidermal growth factor receptor ligands regulate keratin 8 expression in keratinocytes, and transforming growth factor alpha mediates the induction of keratin 8 by the v-rasHa oncogene. Cell Growth Differ. 1993 Apr; 4(4): 317-27. PMID: 7684248, ISSN: 1044-9523.

Untawale S, Zorbas MA, Hodgson CP, Coffey RJ, Gallick GE, North SM, Wildrick DM, Olive M, Blick M, Yeoman LC. Transforming growth factor-alpha production and autoinduction in a colorectal carcinoma cell line (DiFi) with an amplified epidermal growth factor receptor gene. Cancer Res. 1993 Apr 4/1/1993; 53(7): 1630-6. PMID: 8453634, ISSN: 0008-5472.

Olive M, Untawale S, Coffey RJ, Siciliano MJ, Wildrick DM, Fritsche H, Pathak S, Cherry LM, Blick M, Lointier P. Characterization of the DiFi rectal carcinoma cell line derived from a familial adenomatous polyposis patient. In Vitro Cell. Dev. Biol. 1993 Mar; 29A(3 Pt 1): 239-48. PMID: 8385096, ISSN: 0883-8364.

Goldenring JR, Tsunoda Y, Stoch SA, Coffey RJ, Modlin IM. Transforming growth factor-alpha (TGF alpha) inhibition of parietal cell secretion: structural requirements for activity. Regul. Pept. 1993 Jan 1/22/1993; 43(1-2): 37-47. PMID: 8426908, ISSN: 0167-0115.

Romano M, Polk WH, Awad JA, Arteaga CL, Nanney LB, Wargovich MJ, Kraus ER, Boland CR, Coffey RJ. Transforming growth factor alpha protection against drug-induced injury to the rat gastric mucosa in vivo. J. Clin. Invest. 1992 Dec; 90(6): 2409-21. PMID: 1281834, PMCID: PMC443397, DOI: 10.1172/JCI116132, ISSN: 0021-9738.

Coffey RJ, Romano M, Polk WH, Dempsey PJ. Roles for transforming growth factor-alpha in gastric physiology and pathophysiology. Yale J Biol Med. 1992 Nov; 65(6): 693-704; discussion 621. PMID: 1341072, PMCID: PMC2589761, ISSN: 0044-0086.

Coffey RJ, Graves-Deal R, Dempsey PJ, Whitehead RH, Pittelkow MR. Differential regulation of transforming growth factor alpha autoinduction in a nontransformed and transformed epithelial cell. Cell Growth Differ. 1992 Jun; 3(6): 347-54. PMID: 1419897, ISSN: 1044-9523.

Cook PW, Pittelkow MR, Keeble WW, Graves-Deal R, Coffey RJ, Shipley GD. Amphiregulin messenger RNA is elevated in psoriatic epidermis and gastrointestinal carcinomas. Cancer Res. 1992 Jun 6/1/1992; 52(11): 3224-7. PMID: 1591731, ISSN: 0008-5472.

Polk WH, Dempsey PJ, Russell WE, Brown PI, Beauchamp RD, Barnard JA, Coffey RJ. Increased production of transforming growth factor alpha following acute gastric injury. Gastroenterology. 1992 May; 102(5): 1467-74. PMID: 1568557, PII: S0016508592001859, ISSN: 0016-5085.

Halter SA, Dempsey P, Matsui Y, Stokes MK, Graves-Deal R, Hogan BL, Coffey RJ. Distinctive patterns of hyperplasia in transgenic mice with mouse mammary tumor virus transforming growth factor-alpha. Characterization of mammary gland and skin proliferations. Am. J. Pathol. 1992 May; 140(5): 1131-46. PMID: 1316084, PMCID: PMC1886506, ISSN: 0002-9440.

Dempsey PJ, Goldenring JR, Soroka CJ, Modlin IM, McClure RW, Lind CD, Ahlquist DA, Pittelkow MR, Lee DC, Sandgren EP,et al.. Possible role of transforming growth factor alpha in the pathogenesis of M¿¿n¿¿trier¿¿¿s disease: supportive evidence form humans and transgenic mice. 1992.

Coffey RJ, McCutchen CM, Graves-Deal R, Polk WH. Transforming growth factors and related peptides in gastrointestinal neoplasia. J. Cell. Biochem. Suppl. 1992; 16G: 111-8. PMID: 1335097, ISSN: 0733-1959.

Arteaga CL, Coffey RJ. Transforming growth factor-beta isoforms in mammary neoplasia: more questions than answers [editorial]. Hum. Pathol. 1992 Jan; 23(1): 1-3. PMID: 1544663, ISSN: 0046-8177.

Barnard JA, Polk WH, Moses HL, Coffey RJ. Production of transforming growth factor-alpha by normal rat small intestine. Am. J. Physiol. 1991 Dec; 261(6 Pt 1): C994-1000. PMID: 1767826, ISSN: 0002-9513.

Arteaga CL, Johnson MD, Todderud G, Coffey RJ, Carpenter G, Page DL. Elevated content of the tyrosine kinase substrate phospholipase C-gamma 1 in primary human breast carcinomas. Proc. Natl. Acad. Sci. U.S.A. 1991 Dec 12/1/1991; 88(23): 10435-9. PMID: 1683701, PMCID: PMC52943, ISSN: 0027-8424.

Beauchamp RD, Coffey RJ, Lyons RM, Perkett EA, Townsend CM, Moses HL. Human carcinoid cell production of paracrine growth factors that can stimulate fibroblast and endothelial cell growth. Cancer Res. 1991 Oct 10/1/1991; 51(19): 5253-60. PMID: 1913648, ISSN: 0008-5472.

Coffey RJ, Barnard JA. Understanding autocrine growth regulation [editorial]. West. J. Med. 1991 Aug; 155(2): 189-90. PMID: 1926859, PMCID: PMC1002963, ISSN: 0093-0415.

McDonnell S, Navre M, Coffey RJ, Matrisian LM. Expression and localization of the matrix metalloproteinase pump-1 (MMP-7) in human gastric and colon carcinomas. Mol. Carcinog. 1991; 4(6): 527-33. PMID: 1793490, ISSN: 0899-1987.

Rosen CB, Nagorney DM, Wiesner RH, Coffey RJ, LaRusso NF. Cholangiocarcinoma complicating primary sclerosing cholangitis. Ann. Surg. 1991 Jan; 213(1): 21-5. PMID: 1845927, PMCID: PMC1358305, ISSN: 0003-4932.

Cook PW, Coffey RJ, Magun BE, Pittelkow MR, Shipley GD. Expression and regulation of mRNA coding for acidic and basic fibroblast growth factor and transforming growth factor alpha in cells derived from human skin. Mol. Endocrinol. 1990 Sep; 4(9): 1377-85. PMID: 1700283, DOI: 10.1210/mend-4-9-1377, ISSN: 0888-8809.

Arteaga CL, Coffey RJ, Dugger TC, McCutchen CM, Moses HL, Lyons RM. Growth stimulation of human breast cancer cells with anti-transforming growth factor beta antibodies: evidence for negative autocrine regulation by transforming growth factor beta. Cell Growth Differ. 1990 Aug; 1(8): 367-74. PMID: 2177634, ISSN: 1044-9523.

Lewis JJ, Goldenring JR, Modlin IM, Coffey RJ. Inhibition of parietal cell H+ secretion by transforming growth factor alpha: a possible autocrine regulatory mechanism. Surgery. 1990 Aug; 108(2): 220-6; discussion 226. PMID: 2382222, ISSN: 0039-6060.

Beauchamp RD, Lyons RM, Yang EY, Coffey RJ, Moses HL. Expression of and response to growth regulatory peptides by two human pancreatic carcinoma cell lines. Pancreas. 1990 Jul; 5(4): 369-80. PMID: 2199964, ISSN: 0885-3177.

Matsui Y, Halter SA, Holt JT, Hogan BL, Coffey RJ. Development of mammary hyperplasia and neoplasia in MMTV-TGF alpha transgenic mice. Cell. 1990 Jun 6/15/1990; 61(6): 1147-55. PMID: 2161707, PII: 0092-8674(90)90077-R, ISSN: 0092-8674.

Schroy P, Rifkin J, Coffey RJ, Winawer S, Friedman E. Role of transforming growth factor beta 1 in induction of colon carcinoma differentiation by hexamethylene bisacetamide. Cancer Res. 1990 Jan 1/15/1990; 50(2): 261-5. PMID: 2295065, ISSN: 0008-5472.

Coffey RJ, Russell WE, Barnard JA. Pharmacokinetics of TGF beta with emphasis on effects in liver and gut. Ann. N. Y. Acad. Sci. 1990; 593: 285-91. PMID: 2197961, ISSN: 0077-8923.

Bascom CC, Wolfshohl JR, Coffey RJ, Madisen L, Webb NR, Purchio AR, Derynck R, Moses HL. Complex regulation of transforming growth factor beta 1, beta 2, and beta 3 mRNA expression in mouse fibroblasts and keratinocytes by transforming growth factors beta 1 and beta 2. Mol. Cell. Biol. 1989 Dec; 9(12): 5508-15. PMID: 2586525, PMCID: PMC363721, ISSN: 0270-7306.

Beauchamp RD, Barnard JA, McCutchen CM, Cherner JA, Coffey RJ. Localization of transforming growth factor alpha and its receptor in gastric mucosal cells. Implications for a regulatory role in acid secretion and mucosal renewal. J. Clin. Invest. 1989 Sep; 84(3): 1017-23. PMID: 2760208, PMCID: PMC329750, DOI: 10.1172/JCI114223, ISSN: 0021-9738.

Pittelkow MR, Lindquist PB, Abraham RT, Graves-Deal R, Derynck R, Coffey RJ. Induction of transforming growth factor-alpha expression in human keratinocytes by phorbol esters. J. Biol. Chem. 1989 Mar 3/25/1989; 264(9): 5164-71. PMID: 2925687, ISSN: 0021-9258.

Barnard JA, Beauchamp RD, Coffey RJ, Moses HL. Regulation of intestinal epithelial cell growth by transforming growth factor type beta. Proc. Natl. Acad. Sci. U.S.A. 1989 Mar; 86(5): 1578-82. PMID: 2466294, PMCID: PMC286741, ISSN: 0027-8424.

Shipley GD, Keeble WW, Hendrickson JE, Coffey RJ, Pittelkow MR. Growth of normal human keratinocytes and fibroblasts in serum-free medium is stimulated by acidic and basic fibroblast growth factor. J. Cell. Physiol. 1989 Mar; 138(3): 511-8. PMID: 2466852, DOI: 10.1002/jcp.1041380310, ISSN: 0021-9541.

Elder JT, Fisher GJ, Lindquist PB, Bennett GL, Pittelkow MR, Coffey RJ, Ellingsworth L, Derynck R, Voorhees JJ. Overexpression of transforming growth factor alpha in psoriatic epidermis. Science. 1989 Feb 2/10/1989; 243(4892): 811-4. PMID: 2916128, ISSN: 0036-8075.

Skinner MK, Takacs K, Coffey RJ. Transforming growth factor-alpha gene expression and action in the seminiferous tubule: peritubular cell-Sertoli cell interactions. Endocrinology. 1989 Feb; 124(2): 845-54. PMID: 2463904, DOI: 10.1210/endo-124-2-845, ISSN: 0013-7227.

Bascom CC, Sipes NJ, Coffey RJ, Moses HL. Regulation of epithelial cell proliferation by transforming growth factors. J. Cell. Biochem. 1989 Jan; 39(1): 25-32. PMID: 2654145, DOI: 10.1002/jcb.240390104, ISSN: 0730-2312.

Skinner MK, Coffey RJ. Regulation of ovarian cell growth through the local production of transforming growth factor-alpha by theca cells. Endocrinology. 1988 Dec; 123(6): 2632-8. PMID: 3264238, DOI: 10.1210/endo-123-6-2632, ISSN: 0013-7227.

Derynck R, Lindquist PB, Lee A, Wen D, Tamm J, Graycar JL, Rhee L, Mason AJ, Miller DA, Coffey RJ. A new type of transforming growth factor-beta, TGF-beta 3. EMBO J. 1988 Dec 12/1/1988; 7(12): 3737-43. PMID: 3208746, PMCID: PMC454948, ISSN: 0261-4189.

Coffey RJ, Bascom CC, Sipes NJ, Graves-Deal R, Weissman BE, Moses HL. Selective inhibition of growth-related gene expression in murine keratinocytes by transforming growth factor beta. Mol. Cell. Biol. 1988 Aug; 8(8): 3088-93. PMID: 2463471, PMCID: PMC363535, ISSN: 0270-7306.

Russell WE, Coffey RJ, Ouellette AJ, Moses HL. Type beta transforming growth factor reversibly inhibits the early proliferative response to partial hepatectomy in the rat. Proc. Natl. Acad. Sci. U.S.A. 1988 Jul; 85(14): 5126-30. PMID: 3164865, PMCID: PMC281701, ISSN: 0027-8424.

Coffey RJ, Sipes NJ, Bascom CC, Graves-Deal R, Pennington CY, Weissman BE, Moses HL. Growth modulation of mouse keratinocytes by transforming growth factors. Cancer Res. 1988 Mar 3/15/1988; 48(6): 1596-602. PMID: 2449957, ISSN: 0008-5472.

Pittelkow MR, Coffey RJ, Moses HJ. Keratinocytes produce and are regulated by transforming growth factors. Ann. N. Y. Acad. Sci. 1988; 548: 211-24. PMID: 2470296, ISSN: 0077-8923.

Griffin MR, Bergstralh EJ, Coffey RJ, Beart RW, Melton LJ. Predictors of survival after curative resection of carcinoma of the colon and rectum. Cancer. 1987 Nov 11/1/1987; 60(9): 2318-24. PMID: 3440238, ISSN: 0008-543X.

Coffey RJ, Kost LJ, Lyons RM, Moses HL, LaRusso NF. Hepatic processing of transforming growth factor beta in the rat. Uptake, metabolism, and biliary excretion. J. Clin. Invest. 1987 Sep; 80(3): 750-7. PMID: 3476497, PMCID: PMC442299, DOI: 10.1172/JCI113130, ISSN: 0021-9738.

Coffey RJ, Goustin AS, Soderquist AM, Shipley GD, Wolfshohl J, Carpenter G, Moses HL. Transforming growth factor alpha and beta expression in human colon cancer lines: implications for an autocrine model. Cancer Res. 1987 Sep 9/1/1987; 47(17): 4590-4. PMID: 2887281, ISSN: 0008-5472.

Coffey RJ, Derynck R, Wilcox JN, Bringman TS, Goustin AS, Moses HL, Pittelkow MR. Production and auto-induction of transforming growth factor-alpha in human keratinocytes. Nature. 1987 Aug 8/27/1987; 328(6133): 817-20. PMID: 2442615, DOI: 10.1038/328817a0, ISSN: 0028-0836.

Coffey RJ, Leof EB, Shipley GD, Moses HL. Suramin inhibition of growth factor receptor binding and mitogenicity in AKR-2B cells. J. Cell. Physiol. 1987 Jul; 132(1): 143-8. PMID: 3496343, DOI: 10.1002/jcp.1041320120, ISSN: 0021-9541.

Keski-Oja J, Leof EB, Lyons RM, Coffey RJ, Moses HL. Transforming growth factors and control of neoplastic cell growth. J. Cell. Biochem. 1987 Feb; 33(2): 95-107. PMID: 3553215, DOI: 10.1002/jcb.240330204, ISSN: 0730-2312.

Moses HL, Coffey RJ, Leof EB, Lyons RM, Keski-Oja J. Transforming growth factor beta regulation of cell proliferation. J Cell Physiol Suppl. 1987; Suppl 5: 1-7. PMID: 3316252, ISSN: 0737-1462.

Coffey RJ, Shipley GD, Moses HL. Production of transforming growth factors by human colon cancer lines. Cancer Res. 1986 Mar; 46(3): 1164-9. PMID: 3002610, ISSN: 0008-5472.

Coffey RJ, Addison T, Lie J, Cockerill FI. Acquired Immune Deficiency Syndrome (AIDS): Liver Involvement with Cryptococcus neoformans as Presenting Manifestation of Disseminated Cryptococcosis, and a Review of Hepatic Disease in Male Homosexuals. 1986.

Coffey RJ, Go VL, Zinsmeister AR, DiMagno EP. The acute effects of coffee and caffeine on human interdigestive exocrine pancreatic secretion. Pancreas. 1986; 1(1): 55-61. PMID: 3575300, ISSN: 0885-3177.

Moses HL, Tucker RF, Leof EB, Coffey RJ, Halper J and Shipley GD. Type B transforming growth factor is a growth stimulator and a growth inhibitor. 1985.

MacCarty RL, LaRusso NF, May GR, Bender CE, Wiesner RH, King JE and Coffey RJ. Cholangiocarcinoma complicating primary sclerosing cholangitis: cholangiographic appearances. 1985.

Coffey RJ, Jr., Knight CD, Jr., van Heerden JA and Weiland LH. Gastric adenocarcinoma complicating Gardner's syndrome in a North American woman. 1985.

Wee A, Ludwig J, Coffey RJ, Jr., LaRusso NF, Wiesner RH. Hepatobiliary carcinoma associated with primary sclerosing cholangitis and chronic ulcerative colitis. 1985.

Zimmerman SE, Smith FP, Phillips TM, Coffey RJ, Schein PS. Gastric carcinoma and thrombotic thrombocytopenic purpura: association with plasma immune complex concentrations. Br Med J (Clin Res Ed). 1982 May 5/15/1982; 284(6327): 1432-4. PMID: 6805553, PMCID: PMC1498348, ISSN: 0267-0623.

Available Postdoctoral Position Details
Posted: 11/4/2015
Postdoctoral Fellowship Positions are immediately available within the Vanderbilt University Epithelial Biology Center. The Center is dedicated to the multi-disciplinary study of intra- and extra-cellular trafficking and signaling, establishment and maintenance of epithelial polarity, and role of intestinal stem cells in health and disease. Positions are immediately available in NIH-funded research programs to

characterize gastrointestinal stem cells and their role in mouse and human neoplasia. Mouse work will be facilitated using newly developed compartment-selective Cre drivers;

advance the recent discovery that EGF receptor ligands signal via exosomes and that mutant KRAS affects the protein and RNA composition and behavior of exosomes.

Individuals with prior experience in exosomal trafficking and function, mouse modeling, trafficking in polarized epithelial cells, and/or high resolution microscopy are especially encouraged to apply.

Salaries commensurate with previous training. Send CVs to Robert J. Coffey, MD, at