Jin Chen, M.D., Ph.D.

Professor

jin.chen@vanderbilt.edu

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Faculty Appointments
Professor of Medicine Professor of Cancer BiologyProfessor of Cell and Developmental Biology
Education
Ph.D., Biochemistry and Molecular Pharmacology, Harvard University, Cambridge, MassachusettsM.D., Medicine, Shanghai Medical University, Shanghai
Office Address
T-3207E MCN
Vanderbilt University School of Medicine
1161 21st Ave South
Nashville, TN 37232
Research Description
Our major focus has been to characterize the role of Eph receptor tyrosine kinases and their ligands, ephrins, in tumor metabolism, cancer metastasis, and tumor angiogenesis. Our approach involves a combination of oncogenomics and proteomics, confocal microscopy and imaging, transgenic and knock out animal models, and traditional cell biology and biochemistry techniques.

Eph RTKs and their ligands are dysregulated in tumor tissues and expression of these molecules is associated with clinical outcome of various cancer types. In particular, EphA2 receptor plays critical roles in both tumor cells and tumor blood vessels. Our laboratory demonstrated that epithelial EphA2 is required for cell proliferation and tumor initiation. We also showed that vascular endothelial EphA2 promotes tumor progression through angiogenesis. As EphA2 regulates both tumor cells and host microenvironment, it is a good target for cancer therapy. Several anti-EphA2 agents have been developed and we are testing selective small molecule Eph receptor kinase inhibitors. Current projects in the lab include:

1. Role of ephrin-A1, the ligand for EphA2 RTK, in tumor metabolism in breast cancer.
2. EphA2 in K-Ras and TKI-resistant EGFR mutant lung cancer and cancer stem cells.
3. Dissecting opposing roles of EphA2 and EphA3 in lung cancer.
4. Regulation of angiocrine signaling in tumor progression, metastasis, and stem cell function.



Research Keywords
To investigate the role of Eph receptor tyrosine kinases and their ligands (ephrins) in tumor angiogenesis, tumor metabolism, and cancer stem cells, and to develop inhibitors targeting EphA2 receptor in cancer.
Publications
Amato KR, Wang S, Tan L, Hastings AK, Song W, Lovly CM, Meador CB, Ye F, Lu P, Balko JM, Colvin DC, Cates JM, Pao W, Gray NS, Chen J. EPHA2 Blockade Overcomes Acquired Resistance to EGFR Kinase Inhibitors in Lung Cancer. Cancer Res [print-electronic]. 2016 Jan 1/15/2016; 76(2): 305-18. PMID: 26744526, PMCID: PMC4715957, PII: 0008-5472.CAN-15-0717, DOI: 10.1158/0008-5472.CAN-15-0717, ISSN: 1538-7445.

Wang S, Amato KR, Song W, Youngblood V, Lee K, Boothby M, Brantley-Sieders DM, Chen J. Regulation of endothelial cell proliferation and vascular assembly through distinct mTORC2 signaling pathways. Mol. Cell. Biol [print-electronic]. 2015 Apr; 35(7): 1299-313. PMID: 25582201, PMCID: PMC4355541, PII: MCB.00306-14, DOI: 10.1128/MCB.00306-14, ISSN: 1098-5549.

Youngblood V, Wang S, Song W, Walter D, Hwang Y, Chen J, Brantley-Sieders DM. Elevated Slit2 Activity Impairs VEGF-Induced Angiogenesis and Tumor Neovascularization in EphA2-Deficient Endothelium. Mol. Cancer Res [print-electronic]. 2015 Mar; 13(3): 524-37. PMID: 25504371, PMCID: PMC4416411, PII: 1541-7786.MCR-14-0142, DOI: 10.1158/1541-7786.MCR-14-0142, ISSN: 1557-3125.

Chen J, Song W, Amato K. Eph receptor tyrosine kinases in cancer stem cells. Cytokine Growth Factor Rev [print-electronic]. 2015 Feb; 26(1): 1-6. PMID: 24933439, PMCID: PMC4234705, PII: S1359-6101(14)00043-4, DOI: 10.1016/j.cytogfr.2014.05.001, ISSN: 1879-0305.

McCormack DR, Walsh AJ, Sit W, Arteaga CL, Chen J, Cook RS, Skala MC. In vivo hyperspectral imaging of microvessel response to trastuzumab treatment in breast cancer xenografts. Biomed Opt Express. 2014 Jul 7/1/2014; 5(7): 2247-61. PMID: 25071962, PMCID: PMC4102362, PII: 210394, DOI: 10.1364/BOE.5.002247, ISSN: 2156-7085.

Song W, Ma Y, Wang J, Brantley-Sieders D, Chen J. JNK signaling mediates EPHA2-dependent tumor cell proliferation, motility, and cancer stem cell-like properties in non-small cell lung cancer. Cancer Res [print-electronic]. 2014 May 5/1/2014; 74(9): 2444-54. PMID: 24607842, PMCID: PMC4008716, PII: 0008-5472.CAN-13-2136, DOI: 10.1158/0008-5472.CAN-13-2136, ISSN: 1538-7445.

Amato KR, Wang S, Hastings AK, Youngblood VM, Santapuram PR, Chen H, Cates JM, Colvin DC, Ye F, Brantley-Sieders DM, Cook RS, Tan L, Gray NS, Chen J. Genetic and pharmacologic inhibition of EPHA2 promotes apoptosis in NSCLC. J. Clin. Invest [print-electronic]. 2014 May; 124(5): 2037-49. PMID: 24713656, PMCID: PMC4001547, PII: 72522, DOI: 10.1172/JCI72522, ISSN: 1558-8238.

Takahashi K, Matafonov A, Sumarriva K, Ito H, Lauhan C, Zemel D, Tsuboi N, Chen J, Reynolds A, Takahashi T. CD148 tyrosine phosphatase promotes cadherin cell adhesion. PLoS ONE. 2014; 9(11): e112753. PMID: 25386896, PMCID: PMC4227875, PII: PONE-D-14-33624, DOI: 10.1371/journal.pone.0112753, ISSN: 1932-6203.

Boissier P, Chen J, Huynh-Do U. EphA2 signaling following endocytosis: role of Tiam1. Traffic [print-electronic]. 2013 Dec; 14(12): 1255-71. PMID: 24112471, PMCID: PMC3859973, DOI: 10.1111/tra.12123, ISSN: 1600-0854.

Zhuang G, Song W, Amato K, Hwang Y, Lee K, Boothby M, Ye F, Guo Y, Shyr Y, Lin L, Carbone DP, Brantley-Sieders DM, Chen J. Effects of cancer-associated EPHA3 mutations on lung cancer. J. Natl. Cancer Inst [print-electronic]. 2012 Aug 8/8/2012; 104(15): 1182-97. PMID: 22829656, PMCID: PMC3611812, PII: djs297, DOI: 10.1093/jnci/djs297, ISSN: 1460-2105.

Hahn AS, Kaufmann JK, Wies E, Naschberger E, Panteleev-Ivlev J, Schmidt K, Holzer A, Schmidt M, Chen J, König S, Ensser A, Myoung J, Brockmeyer NH, Stürzl M, Fleckenstein B, Neipel F. The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma–associated herpesvirus. Nat. Med. 2012 Jun; 18(6): 961-6. PMID: 22635007, PMCID: PMC3645317, PII: nm.2805, DOI: 10.1038/nm.2805, ISSN: 1546-170X.

Lee K, Nam KT, Cho SH, Gudapati P, Hwang Y, Park DS, Potter R, Chen J, Volanakis E, Boothby M. Vital roles of mTOR complex 2 in Notch-driven thymocyte differentiation and leukemia. J. Exp. Med [print-electronic]. 2012 Apr 4/9/2012; 209(4): 713-28. PMID: 22473959, PMCID: PMC3328370, PII: jem.20111470, DOI: 10.1084/jem.20111470, ISSN: 1540-9538.

Funk SD, Yurdagul A, Albert P, Traylor JG, Jin L, Chen J, Orr AW. EphA2 activation promotes the endothelial cell inflammatory response: a potential role in atherosclerosis. Arterioscler. Thromb. Vasc. Biol [print-electronic]. 2012 Mar; 32(3): 686-95. PMID: 22247258, PMCID: PMC3325141, PII: ATVBAHA.111.242792, DOI: 10.1161/ATVBAHA.111.242792, ISSN: 1524-4636.

Chen J. Regulation of tumor initiation and metastatic progression by Eph receptor tyrosine kinases. Adv. Cancer Res. 2012; 114: 1-20. PMID: 22588054, PMCID: PMC3626259, PII: B978-0-12-386503-8.00001-6, DOI: 10.1016/B978-0-12-386503-8.00001-6, ISSN: 0065-230X.

Rao M, Song W, Jiang A, Shyr Y, Lev S, Greenstein D, Brantley-Sieders D, Chen J. VAMP-associated protein B (VAPB) promotes breast tumor growth by modulation of Akt activity. PLoS ONE [print-electronic]. 2012; 7(10): e46281. PMID: 23049696, PMCID: PMC3462209, PII: PONE-D-12-16139, DOI: 10.1371/journal.pone.0046281, ISSN: 1932-6203.

Brantley-Sieders DM, Dunaway CM, Rao M, Short S, Hwang Y, Gao Y, Li D, Jiang A, Shyr Y, Wu JY, Chen J. Angiocrine factors modulate tumor proliferation and motility through EphA2 repression of Slit2 tumor suppressor function in endothelium. Cancer Res [print-electronic]. 2011 Feb 2/1/2011; 71(3): 976-87. PMID: 21148069, PMCID: PMC3032824, PII: 0008-5472.CAN-10-3396, DOI: 10.1158/0008-5472.CAN-10-3396, ISSN: 1538-7445.

Dunaway CM, Hwang Y, Lindsley CW, Cook RS, Wu JY, Boothby M, Chen J, Brantley-Sieders DM. Cooperative signaling between Slit2 and Ephrin-A1 regulates a balance between angiogenesis and angiostasis. Mol. Cell. Biol [print-electronic]. 2011 Feb; 31(3): 404-16. PMID: 21135133, PMCID: PMC3028625, PII: MCB.00667-10, DOI: 10.1128/MCB.00667-10, ISSN: 1098-5549.

Brantley-Sieders DM, Jiang A, Sarma K, Badu-Nkansah A, Walter DL, Shyr Y, Chen J. Eph/ephrin profiling in human breast cancer reveals significant associations between expression level and clinical outcome. PLoS ONE [print-electronic]. 2011; 6(9): e24426. PMID: 21935409, PMCID: PMC3174170, PII: PONE-D-11-14906, DOI: 10.1371/journal.pone.0024426, ISSN: 1932-6203.

Frieden LA, Townsend TA, Vaught DB, Delaughter DM, Hwang Y, Barnett JV, Chen J. Regulation of heart valve morphogenesis by Eph receptor ligand, ephrin-A1. Dev. Dyn [print-electronic]. 2010 Dec; 239(12): 3226-34. PMID: 20960543, PMCID: PMC3023820, DOI: 10.1002/dvdy.22458, ISSN: 1097-0177.

Min Y, Ren X, Vaught DB, Chen J, Donnelly E, Lynch CC, Lin PC. Tie2 signaling regulates osteoclastogenesis and osteolytic bone invasion of breast cancer. Cancer Res [print-electronic]. 2010 Apr 4/1/2010; 70(7): 2819-28. PMID: 20233869, PMCID: PMC2848896, PII: 0008-5472.CAN-09-1915, DOI: 10.1158/0008-5472.CAN-09-1915, ISSN: 1538-7445.

Zhuang G, Brantley-Sieders DM, Vaught D, Yu J, Xie L, Wells S, Jackson D, Muraoka-Cook R, Arteaga C, Chen J. Elevation of receptor tyrosine kinase EphA2 mediates resistance to trastuzumab therapy. Cancer Res [print-electronic]. 2010 Jan 1/1/2010; 70(1): 299-308. PMID: 20028874, PMCID: PMC3859619, PII: 0008-5472.CAN-09-1845, DOI: 10.1158/0008-5472.CAN-09-1845, ISSN: 1538-7445.

Brantley-Sieders DM, Zhuang G, Vaught D, Freeman T, Hwang Y, Hicks D, Chen J. Host deficiency in Vav2/3 guanine nucleotide exchange factors impairs tumor growth, survival, and angiogenesis in vivo. Mol. Cancer Res [print-electronic]. 2009 May; 7(5): 615-23. PMID: 19435813, PMCID: PMC2739740, PII: 1541-7786.MCR-08-0401, DOI: 10.1158/1541-7786.MCR-08-0401, ISSN: 1541-7786.

Vaught D, Chen J, Brantley-Sieders DM. Regulation of mammary gland branching morphogenesis by EphA2 receptor tyrosine kinase. Mol. Biol. Cell [print-electronic]. 2009 May; 20(10): 2572-81. PMID: 19321667, PMCID: PMC2682598, PII: E08-04-0378, DOI: 10.1091/mbc.E08-04-0378, ISSN: 1939-4586.

Bogan C, Chen J, O'Sullivan MG, Cormier RT. Loss of EphA2 receptor tyrosine kinase reduces ApcMin/+ tumorigenesis. Int. J. Cancer. 2009 Mar 3/15/2009; 124(6): 1366-71. PMID: 19089910, DOI: 10.1002/ijc.24083, ISSN: 1097-0215.

Chen J, Zhuang G, Frieden L, Debinski W. Eph receptors and Ephrins in cancer: common themes and controversies. Cancer Res. 2008 Dec 12/15/2008; 68(24): 10031-3. PMID: 19074866, PMCID: PMC2605675, PII: 68/24/10031, DOI: 10.1158/0008-5472.CAN-08-3010, ISSN: 1538-7445.

Fang WB, Brantley-Sieders DM, Hwang Y, Ham AJ, Chen J. Identification and functional analysis of phosphorylated tyrosine residues within EphA2 receptor tyrosine kinase. J. Biol. Chem [print-electronic]. 2008 Jun 6/6/2008; 283(23): 16017-26. PMID: 18387945, PMCID: PMC2414276, PII: M709934200, DOI: 10.1074/jbc.M709934200, ISSN: 0021-9258.

Fang WB, Ireton RC, Zhuang G, Takahashi T, Reynolds A, Chen J. Overexpression of EPHA2 receptor destabilizes adherens junctions via a RhoA-dependent mechanism. J. Cell. Sci [print-electronic]. 2008 Feb 2/1/2008; 121(Pt 3): 358-68. PMID: 18198190, PII: jcs.017145, DOI: 10.1242/jcs.017145, ISSN: 0021-9533.

Brantley-Sieders DM, Zhuang G, Hicks D, Fang WB, Hwang Y, Cates JM, Coffman K, Jackson D, Bruckheimer E, Muraoka-Cook RS, Chen J. The receptor tyrosine kinase EphA2 promotes mammary adenocarcinoma tumorigenesis and metastatic progression in mice by amplifying ErbB2 signaling. J. Clin. Invest. 2008 Jan; 118(1): 64-78. PMID: 18079969, PMCID: PMC2129239, DOI: 10.1172/JCI33154, ISSN: 0021-9738.

Vaught D, Brantley-Sieders DM, Chen J. Eph receptors in breast cancer: roles in tumor promotion and tumor suppression. Breast Cancer Res [print-electronic]. 2008; 10(6): 217. PMID: 19144211, PMCID: PMC2656900, PII: bcr2207, DOI: 10.1186/bcr2207, ISSN: 1465-542X.

Zhuang G, Hunter S, Hwang Y, Chen J. Regulation of EphA2 receptor endocytosis by SHIP2 lipid phosphatase via phosphatidylinositol 3-Kinase-dependent Rac1 activation. J. Biol. Chem [print-electronic]. 2007 Jan 1/26/2007; 282(4): 2683-94. PMID: 17135240, PII: M608509200, DOI: 10.1074/jbc.M608509200, ISSN: 0021-9258.

Brantley-Sieders DM, Fang WB, Hwang Y, Hicks D, Chen J. Ephrin-A1 facilitates mammary tumor metastasis through an angiogenesis-dependent mechanism mediated by EphA receptor and vascular endothelial growth factor in mice. Cancer Res. 2006 Nov 11/1/2006; 66(21): 10315-24. PMID: 17079451, PII: 66/21/10315, DOI: 10.1158/0008-5472.CAN-06-1560, ISSN: 0008-5472.

Brissova M, Shostak A, Shiota M, Wiebe PO, Poffenberger G, Kantz J, Chen Z, Carr C, Jerome WG, Chen J, Baldwin HS, Nicholson W, Bader DM, Jetton T, Gannon M, Powers AC. Pancreatic islet production of vascular endothelial growth factor--a is essential for islet vascularization, revascularization, and function. Diabetes. 2006 Nov; 55(11): 2974-85. PMID: 17065333, PII: 55/11/2974, DOI: 10.2337/db06-0690, ISSN: 0012-1797.

Hunter SG, Zhuang G, Brantley-Sieders D, Swat W, Cowan CW, Chen J. Essential role of Vav family guanine nucleotide exchange factors in EphA receptor-mediated angiogenesis. Mol. Cell. Biol. 2006 Jul; 26(13): 4830-42. PMID: 16782872, PMCID: PMC1489141, PII: 26/13/4830, DOI: 10.1128/MCB.02215-05, ISSN: 0270-7306.

Chen J, Hicks D, Brantley-Sieders D, Cheng N, McCollum GW, Qi-Werdich X, Penn J. Inhibition of retinal neovascularization by soluble EphA2 receptor. Exp. Eye Res [print-electronic]. 2006 Apr; 82(4): 664-73. PMID: 16359662, PII: S0014-4835(05)00293-9, DOI: 10.1016/j.exer.2005.09.004, ISSN: 0014-4835.

Fang WB, Brantley-Sieders DM, Parker MA, Reith AD, Chen J. A kinase-dependent role for EphA2 receptor in promoting tumor growth and metastasis. Oncogene. 2005 Nov 11/24/2005; 24(53): 7859-68. PMID: 16103880, PII: 1208937, DOI: 10.1038/sj.onc.1208937, ISSN: 0950-9232.

Brantley-Sieders DM, Fang WB, Hicks DJ, Zhuang G, Shyr Y, Chen J. Impaired tumor microenvironment in EphA2-deficient mice inhibits tumor angiogenesis and metastatic progression. FASEB J [print-electronic]. 2005 Nov; 19(13): 1884-6. PMID: 16166198, PII: 05-4038fje, DOI: 10.1096/fj.05-4038fje, ISSN: 1530-6860.

Ireton RC, Chen J. EphA2 receptor tyrosine kinase as a promising target for cancer therapeutics. Curr Cancer Drug Targets. 2005 May; 5(3): 149-57. PMID: 15892616, ISSN: 1568-0096.

Parker M, Roberts R, Enriquez M, Zhao X, Takahashi T, Pat Cerretti D, Daniel T, Chen J. Reverse endocytosis of transmembrane ephrin-B ligands via a clathrin-mediated pathway. Biochem. Biophys. Res. Commun. 2004 Oct 10/8/2004; 323(1): 17-23. PMID: 15351694, PII: S0006-291X(04)01590-6, DOI: 10.1016/j.bbrc.2004.07.209, ISSN: 0006-291X.

Brantley-Sieders DM, Caughron J, Hicks D, Pozzi A, Ruiz JC, Chen J. EphA2 receptor tyrosine kinase regulates endothelial cell migration and vascular assembly through phosphoinositide 3-kinase-mediated Rac1 GTPase activation. J. Cell. Sci [print-electronic]. 2004 Apr 4/15/2004; 117(Pt 10): 2037-49. PMID: 15054110, PII: jcs.01061, DOI: 10.1242/jcs.01061, ISSN: 0021-9533.

Brantley-Sieders DM, Chen J. Eph receptor tyrosine kinases in angiogenesis: from development to disease. Angiogenesis. 2004; 7(1): 17-28. PMID: 15302992, PII: 5272546, DOI: 10.1023/B:AGEN.0000037340.33788.87, ISSN: 0969-6970.

Brantley-Sieders D, Schmidt S, Parker M, Chen J. Eph receptor tyrosine kinases in tumor and tumor microenvironment. Curr. Pharm. Des. 2004; 10(27): 3431-42. PMID: 15544526, ISSN: 1381-6128.

Cheng N, Brantley D, Fang WB, Liu H, Fanslow W, Cerretti DP, Bussell KN, Reith A, Jackson D, Chen J. Inhibition of VEGF-dependent multistage carcinogenesis by soluble EphA receptors. Neoplasia. 2003 Sep; 5(5): 445-56. PMID: 14670182, PMCID: PMC1502614, ISSN: 1522-8002.

DeBusk LM, Chen Y, Nishishita T, Chen J, Thomas JW, Lin PC. Tie2 receptor tyrosine kinase, a major mediator of tumor necrosis factor alpha-induced angiogenesis in rheumatoid arthritis. Arthritis Rheum. 2003 Sep; 48(9): 2461-71. PMID: 13130465, DOI: 10.1002/art.11213, ISSN: 0004-3591.

Cheng N, Brantley DM, Liu H, Lin Q, Enriquez M, Gale N, Yancopoulos G, Cerretti DP, Daniel TO, Chen J. Blockade of EphA receptor tyrosine kinase activation inhibits vascular endothelial cell growth factor-induced angiogenesis. Mol. Cancer Res. 2002 Nov; 1(1): 2-11. PMID: 12496364, ISSN: 1541-7786.

Brantley DM, Cheng N, Thompson EJ, Lin Q, Brekken RA, Thorpe PE, Muraoka RS, Cerretti DP, Pozzi A, Jackson D, Lin C, Chen J. Soluble Eph A receptors inhibit tumor angiogenesis and progression in vivo. Oncogene. 2002 Oct 10/10/2002; 21(46): 7011-26. PMID: 12370823, DOI: 10.1038/sj.onc.1205679, ISSN: 0950-9232.

Huynh-Do U, Vindis C, Liu H, Cerretti DP, McGrew JT, Enriquez M, Chen J, Daniel TO. Ephrin-B1 transduces signals to activate integrin-mediated migration, attachment and angiogenesis. J. Cell. Sci. 2002 Aug 8/1/2002; 115(Pt 15): 3073-81. PMID: 12118063, ISSN: 0021-9533.

Muraoka RS, Dumont N, Ritter CA, Dugger TC, Brantley DM, Chen J, Easterly E, Roebuck LR, Ryan S, Gotwals PJ, Koteliansky V, Arteaga CL. Blockade of TGF-beta inhibits mammary tumor cell viability, migration, and metastases. J. Clin. Invest. 2002 Jun; 109(12): 1551-9. PMID: 12070302, PMCID: PMC151012, DOI: 10.1172/JCI15234, ISSN: 0021-9738.

Chen Y, Rosloniec E, Price J, Boothby M, Chen J. Constitutive expression of BCL-X(L) in the T lineage attenuates collagen-induced arthritis in Bcl-X(L) transgenic mice. Arthritis Rheum. 2002 Feb; 46(2): 514-21. PMID: 11840455, PII: 10.1002/art.10128, ISSN: 0004-3591.

Cheng N, Brantley DM, Chen J. The ephrins and Eph receptors in angiogenesis. Cytokine Growth Factor Rev. 2002 Feb; 13(1): 75-85. PMID: 11750881, PII: S1359610101000314, ISSN: 1359-6101.

Jo D, Nashabi A, Doxsee C, Lin Q, Unutmaz D, Chen J, Ruley HE. Epigenetic regulation of gene structure and function with a cell-permeable Cre recombinase. Nat. Biotechnol. 2001 Oct; 19(10): 929-33. PMID: 11581657, PII: nbt1001-929, DOI: 10.1038/nbt1001-929, ISSN: 1087-0156.

Cheng N, Chen J. Tumor necrosis factor-alpha induction of endothelial ephrin A1 expression is mediated by a p38 MAPK- and SAPK/JNK-dependent but nuclear factor-kappa B-independent mechanism. J. Biol. Chem [print-electronic]. 2001 Apr 4/27/2001; 276(17): 13771-7. PMID: 11278471, PII: M009147200, DOI: 10.1074/jbc.M009147200, ISSN: 0021-9258.

Chen Y, Rosloniec E, Goral MI, Boothby M, Chen J. Redirection of T cell effector function in vivo and enhanced collagen-induced arthritis mediated by an IL-2R beta/IL-4R alpha chimeric cytokine receptor transgene. J. Immunol. 2001 Mar 3/15/2001; 166(6): 4163-9. PMID: 11238667, ISSN: 0022-1767.

Seetharaman R, Mora AL, Nabozny G, Boothby M, Chen J. Essential role of T cell NF-kappa B activation in collagen-induced arthritis. J. Immunol. 1999 Aug 8/1/1999; 163(3): 1577-83. PMID: 10415062, PII: ji_v163n3p1577, ISSN: 0022-1767.

Youn J, Chen J, Goenka S, Aronica MA, Mora AL, Correa V, Sheller JR, Boothby M. In vivo function of an interleukin 2 receptor beta chain (IL-2Rbeta)/IL-4Ralpha cytokine receptor chimera potentiates allergic airway disease. J. Exp. Med. 1998 Nov 11/16/1998; 188(10): 1803-16. PMID: 9815258, PMCID: PMC2212401, ISSN: 0022-1007.

Chen J, Ruley HE. An enhancer element in the EphA2 (Eck) gene sufficient for rhombomere-specific expression is activated by HOXA1 and HOXB1 homeobox proteins. J. Biol. Chem. 1998 Sep 9/18/1998; 273(38): 24670-5. PMID: 9733765, ISSN: 0021-9258.

G. Hicks, J. Chen, and H.E. Ruley. Production and use of Retroviruses. Culture of Specialised Cells Series. 1998; 4.

Chen J, Nachabah A, Scherer C, Ganju P, Reith A, Bronson R, Ruley HE. Germ-line inactivation of the murine Eck receptor tyrosine kinase by gene trap retroviral insertion. Oncogene. 1996 Mar 3/7/1996; 12(5): 979-88. PMID: 8649815, ISSN: 0950-9232.

C. Scherer, J. Chen, A. Nachebeh, N. Hopkins and E. Ruley (First two authors contributed equally.). Transcriptional specificity of totipotent embryonic stem cell. Cell Growth and Differentiation. 1996; 7: 1393-401.

Hicks GG, Shi EG, Chen J, Roshon M, Williamson D, Scherer C, Ruley HE. Retrovirus gene traps. Meth. Enzymol. 1995; 254: 263-75. PMID: 8531691, ISSN: 0076-6879.

J. Chen, J. DeGregori, G. Hicks, M. Roshon, E-G Shi, C. Scherer and E. Ruley. Gene Trap Retrovirus. Meth. Mol. Genet. 1994; 4: 123-40.

S. Lin, W. Long, J. Chen, and N. Hopkins. Production of germ line chimeras in zebrafish by cell transplants from genetically pigmented to albino embryos. Proc. Natl. Acad. Sci. USA. 1992; 89: 4519-23.

J. Chen, G. W. Rebeck, B. Derfler, and L. Samson. The expression of bacterial DNA repair genes in eukaryotic cells; and vice versa. Anticarcinogenesis and Radiation Protection (ed. O. F. Nygaard and A.C. Upton) Plenum Press, New York. 1991; 47-56.

J. Chen, and L. Samson. Induction of S. cerevisiae MAG 3MeA DNA glycosylase transcript levels in response to DNA damage. Nucleic Acids Res. 1991; 19: 6427-32.

W. Xiao, B. Derfler, J. Chen, and L. Samson. Primary sequence and biological functions of a S. cerevisiae 06-methylguanine/04-methylthymine DNA repair methyltransferase gene. EMBO J. 1991; 10: 2719-2186.

J. Chen, B. Derfler, and L. Samson. Saccharomyces cerevisiae 3-methyladenine DNA glycosylase has homology to the AlkA glycosylase of E. coli and is induced in response to DNA alkylation damage. EMBO J. 1990; 9: 4569-75.

J. Chen, B. Derfler, A. Maskati, and L. Samson. Cloning a eukaryotic DNA glycosylase repair gene by the suppression of a DNA repair defect in E. coli. Proc. Natl. Acad. Sci. USA. 1989; 86: 7961-5.

Available Postdoctoral Position Details
A postdoctoral position is open for candidates that are eligible for training grant (i.e., a recent Ph.D. who is citizen or permanent resident graduated within 1-2 years). Please send CV and three references to jin.chen@vanderbilt.edu.