Tina M. Iverson, Ph.D.

Associate Professor

tina.iverson@vanderbilt.edu

Visit Lab Site


Faculty Appointments
Associate Professor of Pharmacology Associate Professor of Biochemistry
Education
Ph.D., Biochemistry, California Institute of Technology, Pasadena, CaliforniaB.S., Chemistry, St. John's University, Jamaica, New York
Office Address
460 RRB; Department of Pharmacology
Vanderbilt University Medical Center
23rd Ave @ Pierce
Nashville, TN 37232-6600
Research Description
In humans, transmembrane signaling coordinates tissue and organ function. Inappropriate signaling is associated with numerous diseases, such as heart disease and cancer. All known transmembrane signaling processes require the formation of transient complexes between a membrane-spanning protein and an intracellular binding partner. These transient complexes are similar in some respects to transition states in chemical reactions, i.e. they are unstable, but essential for signaling to occur. Understanding the molecular details of these complexes is important for a complete view of biological signaling. To reveal how information is transferred across a membrane, we are performing x-ray crystallographic analysis of several model systems.

Research Keywords
Structural basis for molecular recognition
Publications
McCulloch KM, McCranie EK, Smith JA, Sarwar M, Mathieu JL, Gitschlag BL, Du Y, Bachmann BO, Iverson TM. Oxidative cyclizations in orthosomycin biosynthesis expand the known chemistry of an oxygenase superfamily. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2015 Sep 9/15/2015; 112(37): 11547-52. PMID: 26240321, PMCID: PMC4577193, PII: 1500964112, DOI: 10.1073/pnas.1500964112, ISSN: 1091-6490.

Chen Q, Vishnivetskiy SA, Zhuang T, Cho MK, Thaker TM, Sanders CR, Gurevich VV, Iverson TM. The rhodopsin-arrestin-1 interaction in bicelles. Methods Mol. Biol. 2015; 1271: 77-95. PMID: 25697518, PMCID: PMC4520306, DOI: 10.1007/978-1-4939-2330-4_6, ISSN: 1940-6029.

Kaya AI, Iverson TM, Hamm HE. Functional stability of rhodopsin in a bicelle system: evaluating G protein activation by rhodopsin in bicelles. Methods Mol. Biol. 2015; 1271: 67-76. PMID: 25697517, DOI: 10.1007/978-1-4939-2330-4_5, ISSN: 1940-6029.

Kaya AI, Lokits AD, Gilbert JA, Iverson TM, Meiler J, Hamm HE. A conserved phenylalanine as a relay between the a5 helix and the GDP binding region of heterotrimeric Gi protein a subunit. J. Biol. Chem [print-electronic]. 2014 Aug 8/29/2014; 289(35): 24475-87. PMID: 25037222, PMCID: PMC4148873, PII: M114.572875, DOI: 10.1074/jbc.M114.572875, ISSN: 1083-351X.

Birmingham WR, Starbird CA, Panosian TD, Nannemann DP, Iverson TM, Bachmann BO. Bioretrosynthetic construction of a didanosine biosynthetic pathway. Nat. Chem. Biol [print-electronic]. 2014 May; 10(5): 392-9. PMID: 24657930, PMCID: PMC4017637, PII: nchembio.1494, DOI: 10.1038/nchembio.1494, ISSN: 1552-4469.

Thaker TM, Sarwar M, Preininger AM, Hamm HE, Iverson TM. A transient interaction between the phosphate binding loop and switch I contributes to the allosteric network between receptor and nucleotide in Gai1. J. Biol. Chem [print-electronic]. 2014 Apr 4/18/2014; 289(16): 11331-41. PMID: 24596087, PMCID: PMC4036270, PII: M113.539064, DOI: 10.1074/jbc.M113.539064, ISSN: 1083-351X.

Vishnivetskiy SA, Zhan X, Chen Q, Iverson TM, Gurevich VV. Arrestin expression in E. coli and purification. Curr Protoc Pharmacol. 2014; 67: Unit 2.11.. PMID: 25446290, PMCID: PMC4260927, DOI: 10.1002/0471141755.ph0211s67, ISSN: 1934-8290.

Seo HS, Minasov G, Seepersaud R, Doran KS, Dubrovska I, Shuvalova L, Anderson WF, Iverson TM, Sullam PM. Characterization of fibrinogen binding by glycoproteins Srr1 and Srr2 of Streptococcus agalactiae. J. Biol. Chem [print-electronic]. 2013 Dec 12/13/2013; 288(50): 35982-96. PMID: 24165132, PMCID: PMC3861647, PII: M113.513358, DOI: 10.1074/jbc.M113.513358, ISSN: 1083-351X.

Singh PK, Sarwar M, Maklashina E, Kotlyar V, Rajagukguk S, Tomasiak TM, Cecchini G, Iverson TM. Plasticity of the quinone-binding site of the complex II homolog quinol:fumarate reductase. J. Biol. Chem [print-electronic]. 2013 Aug 8/23/2013; 288(34): 24293-301. PMID: 23836905, PMCID: PMC3750132, PII: M113.487082, DOI: 10.1074/jbc.M113.487082, ISSN: 1083-351X.

Iverson TM. Catalytic mechanisms of complex II enzymes: a structural perspective. Biochim. Biophys. Acta [print-electronic]. 2013 May; 1827(5): 648-57. PMID: 22995215, PMCID: PMC3537904, PII: S0005-2728(12)01031-6, DOI: 10.1016/j.bbabio.2012.09.008, ISSN: 0006-3002.

Vishnivetskiy SA, Chen Q, Palazzo MC, Brooks EK, Altenbach C, Iverson TM, Hubbell WL, Gurevich VV. Engineering visual arrestin-1 with special functional characteristics. J. Biol. Chem [print-electronic]. 2013 Feb 2/1/2013; 288(5): 3394-405. PMID: 23250748, PMCID: PMC3561558, PII: M112.445437, DOI: 10.1074/jbc.M112.445437, ISSN: 1083-351X.

Thaker TM, Tanabe M, Fowler ML, Preininger AM, Ingram-Smith C, Smith KS, Iverson TM. Crystal structures of acetate kinases from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans. J. Struct. Biol [print-electronic]. 2013 Feb; 181(2): 185-9. PMID: 23159802, PMCID: PMC3565045, PII: S1047-8477(12)00313-9, DOI: 10.1016/j.jsb.2012.11.001, ISSN: 1095-8657.

Zhuang T, Chen Q, Cho MK, Vishnivetskiy SA, Iverson TM, Gurevich VV, Sanders CR. Involvement of distinct arrestin-1 elements in binding to different functional forms of rhodopsin. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 Jan 1/15/2013; 110(3): 942-7. PMID: 23277586, PMCID: PMC3549108, PII: 1215176110, DOI: 10.1073/pnas.1215176110, ISSN: 1091-6490.

Iverson TM, Maklashina E, Cecchini G. Structural basis for malfunction in complex II. J. Biol. Chem [print-electronic]. 2012 Oct 10/12/2012; 287(42): 35430-8. PMID: 22904323, PMCID: PMC3471735, PII: R112.408419, DOI: 10.1074/jbc.R112.408419, ISSN: 1083-351X.

Iverson TM, Panosian TD, Birmingham WR, Nannemann DP, Bachmann BO. Molecular differences between a mutase and a phosphatase: investigations of the activation step in Bacillus cereus phosphopentomutase. Biochemistry [print-electronic]. 2012 Mar 3/6/2012; 51(9): 1964-75. PMID: 22329805, PMCID: PMC3302354, DOI: 10.1021/bi201761h, ISSN: 1520-4995.

Thaker TM, Kaya AI, Preininger AM, Hamm HE, Iverson TM. Allosteric mechanisms of G protein-Coupled Receptor signaling: a structural perspective. Methods Mol. Biol. 2012; 796: 133-74. PMID: 22052489, PMCID: PMC3549666, DOI: 10.1007/978-1-61779-334-9_8, ISSN: 1940-6029.

Pyburn TM, Bensing BA, Xiong YQ, Melancon BJ, Tomasiak TM, Ward NJ, Yankovskaya V, Oliver KM, Cecchini G, Sulikowski GA, Tyska MJ, Sullam PM, Iverson TM. A structural model for binding of the serine-rich repeat adhesin GspB to host carbohydrate receptors. PLoS Pathog [print-electronic]. 2011 Jul; 7(7): e1002112. PMID: 21765814, PMCID: PMC3131266, PII: PPATHOGENS-D-11-00123, DOI: 10.1371/journal.ppat.1002112, ISSN: 1553-7374.

Kaya AI, Thaker TM, Preininger AM, Iverson TM, Hamm HE. Coupling efficiency of rhodopsin and transducin in bicelles. Biochemistry [print-electronic]. 2011 Apr 4/19/2011; 50(15): 3193-203. PMID: 21375271, PMCID: PMC3119548, DOI: 10.1021/bi200037j, ISSN: 1520-4995.

Panosian TD, Nannemann DP, Watkins GR, Phelan VV, McDonald WH, Wadzinski BE, Bachmann BO, Iverson TM. Bacillus cereus phosphopentomutase is an alkaline phosphatase family member that exhibits an altered entry point into the catalytic cycle. J. Biol. Chem [print-electronic]. 2011 Mar 3/11/2011; 286(10): 8043-54. PMID: 21193409, PMCID: PMC3048691, PII: M110.201350, DOI: 10.1074/jbc.M110.201350, ISSN: 1083-351X.

Kuchtey J, Olson LM, Rinkoski T, Mackay EO, Iverson TM, Gelatt KN, Haines JL, Kuchtey RW. Mapping of the disease locus and identification of ADAMTS10 as a candidate gene in a canine model of primary open angle glaucoma. PLoS Genet [print-electronic]. 2011 Feb; 7(2): e1001306. PMID: 21379321, PMCID: PMC3040645, DOI: 10.1371/journal.pgen.1001306, ISSN: 1553-7404.

Tomasiak TM, Archuleta TL, Andréll J, Luna-Chávez C, Davis TA, Sarwar M, Ham AJ, McDonald WH, Yankovskaya V, Stern HA, Johnston JN, Maklashina E, Cecchini G, Iverson TM. Geometric restraint drives on- and off-pathway catalysis by the Escherichia coli menaquinol:fumarate reductase. J. Biol. Chem [print-electronic]. 2011 Jan 1/28/2011; 286(4): 3047-56. PMID: 21098488, PMCID: PMC3024798, PII: M110.192849, DOI: 10.1074/jbc.M110.192849, ISSN: 1083-351X.

Vey JL, Al-Mestarihi A, Hu Y, Funk MA, Bachmann BO, Iverson TM. Structure and mechanism of ORF36, an amino sugar oxidizing enzyme in everninomicin biosynthesis. Biochemistry. 2010 Nov 11/2/2010; 49(43): 9306-17. PMID: 20866105, PMCID: PMC2964426, DOI: 10.1021/bi101336u, ISSN: 1520-4995.

Pyburn TM, Yankovskaya V, Bensing BA, Cecchini G, Sullam PM, Iverson TM. Purification, crystallization and preliminary X-ray diffraction analysis of the carbohydrate-binding region of the Streptococcus gordonii adhesin GspB. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun [print-electronic]. 2010 Nov 11/1/2010; 66(Pt 11): 1503-7. PMID: 21045307, PMCID: PMC3001660, PII: S1744309110036535, DOI: 10.1107/S1744309110036535, ISSN: 1744-3091.

Panosian TD, Nannemann DP, Bachmann BO, Iverson TM. Crystallization and preliminary X-ray analysis of a phosphopentomutase from Bacillus cereus. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun [print-electronic]. 2010 Jul 7/1/2010; 66(Pt 7): 811-4. PMID: 20606280, PMCID: PMC2898468, PII: S1744309110017549, DOI: 10.1107/S1744309110017549, ISSN: 1744-3091.

Tanabe M, Nimigean CM, Iverson TM. Structural basis for solute transport, nucleotide regulation, and immunological recognition of Neisseria meningitidis PorB. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2010 Apr 4/13/2010; 107(15): 6811-6. PMID: 20351243, PMCID: PMC2872391, PII: 0912115107, DOI: 10.1073/pnas.0912115107, ISSN: 1091-6490.

Thompson AN, Kim I, Panosian TD, Iverson TM, Allen TW, Nimigean CM. Mechanism of potassium-channel selectivity revealed by Na(+) and Li(+) binding sites within the KcsA pore. Nat. Struct. Mol. Biol [print-electronic]. 2009 Dec; 16(12): 1317-24. PMID: 19946269, PMCID: PMC2825899, PII: nsmb.1703, DOI: 10.1038/nsmb.1703, ISSN: 1545-9985.

Tanabe M, Iverson TM. Expression, purification and preliminary X-ray analysis of the Neisseria meningitidis outer membrane protein PorB. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun [print-electronic]. 2009 Oct 10/1/2009; 65(Pt 10): 996-1000. PMID: 19851005, PMCID: PMC2765884, PII: S1744309109032333, DOI: 10.1107/S1744309109032333, ISSN: 1744-3091.

Preininger AM, Funk MA, Oldham WM, Meier SM, Johnston CA, Adhikary S, Kimple AJ, Siderovski DP, Hamm HE, Iverson TM. Helix dipole movement and conformational variability contribute to allosteric GDP release in Galphai subunits. Biochemistry. 2009 Mar 3/31/2009; 48(12): 2630-42. PMID: 19222191, PMCID: PMC2736342, PII: 10.1021/bi801853a, DOI: 10.1021/bi801853a, ISSN: 1520-4995.

Adler DH, Phillips JA, Cogan JD, Iverson TM, Schnetz-Boutaud N, Stein JA, Brenner DA, Milne GL, Morrow JD, Boutaud O, Oates JA. The enteropathy of prostaglandin deficiency. J. Gastroenterol [print-electronic]. 2009; 44 Suppl 19: 1-7. PMID: 19148786, PMCID: PMC2799331, DOI: 10.1007/s00535-008-2253-y, ISSN: 0944-1174.

Adler DH, Cogan JD, Phillips JA, Schnetz-Boutaud N, Milne GL, Iverson T, Stein JA, Brenner DA, Morrow JD, Boutaud O, Oates JA. Inherited human cPLA(2alpha) deficiency is associated with impaired eicosanoid biosynthesis, small intestinal ulceration, and platelet dysfunction. J. Clin. Invest. 2008 Jun; 118(6): 2121-31. PMID: 18451993, PMCID: PMC2350426, DOI: 10.1172/JCI30473, ISSN: 0021-9738.

Tomasiak TM, Maklashina E, Cecchini G, Iverson TM. A threonine on the active site loop controls transition state formation in Escherichia coli respiratory complex II. J. Biol. Chem [print-electronic]. 2008 May 5/30/2008; 283(22): 15460-8. PMID: 18385138, PMCID: PMC2397489, PII: M801372200, DOI: 10.1074/jbc.M801372200, ISSN: 0021-9258.

Maklashina E, Iverson TM, Sher Y, Kotlyar V, Andréll J, Mirza O, Hudson JM, Armstrong FA, Rothery RA, Weiner JH, Cecchini G. Fumarate reductase and succinate oxidase activity of Escherichia coli complex II homologs are perturbed differently by mutation of the flavin binding domain. J. Biol. Chem [print-electronic]. 2006 Apr 4/21/2006; 281(16): 11357-65. PMID: 16484232, PII: M512544200, DOI: 10.1074/jbc.M512544200, ISSN: 0021-9258.

Iverson TM. Evolution and unique bioenergetic mechanisms in oxygenic photosynthesis. Curr Opin Chem Biol [print-electronic]. 2006 Apr; 10(2): 91-100. PMID: 16504567, PII: S1367-5931(06)00031-7, DOI: 10.1016/j.cbpa.2006.02.013, ISSN: 1367-5931.

Ferreira KN, Iverson TM, Maghlaoui K, Barber J, Iwata S. Architecture of the photosynthetic oxygen-evolving center. Science [print-electronic]. 2004 Mar 3/19/2004; 303(5665): 1831-8. PMID: 14764885, PII: 1093087, DOI: 10.1126/science.1093087, ISSN: 1095-9203.

Cecchini G, Maklashina E, Yankovskaya V, Iverson TM, Iwata S. Variation in proton donor/acceptor pathways in succinate:quinone oxidoreductases. FEBS Lett. 2003 Jun 6/12/2003; 545(1): 31-8. PMID: 12788489, PII: S0014579303003909, ISSN: 0014-5793.

Doukov TI, Iverson TM, Seravalli J, Ragsdale SW, Drennan CL. A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase. Science. 2002 Oct 10/18/2002; 298(5593): 567-72. PMID: 12386327, PII: 298/5593/567, DOI: 10.1126/science.1075843, ISSN: 1095-9203.

Iyer R, Iverson TM, Accardi A, Miller C. A biological role for prokaryotic ClC chloride channels. Nature. 2002 Oct 10/17/2002; 419(6908): 715-8. PMID: 12384697, PII: nature01000, DOI: 10.1038/nature01000, ISSN: 0028-0836.

Iverson TM, Luna-Chavez C, Croal LR, Cecchini G, Rees DC. Crystallographic studies of the Escherichia coli quinol-fumarate reductase with inhibitors bound to the quinol-binding site. J. Biol. Chem [print-electronic]. 2002 May 5/3/2002; 277(18): 16124-30. PMID: 11850430, PII: M200815200, DOI: 10.1074/jbc.M200815200, ISSN: 0021-9258.

Iverson TM, Arciero DM, Hooper AB, Rees DC. High-resolution structures of the oxidized and reduced states of cytochrome c554 from Nitrosomonas europaea. J. Biol. Inorg. Chem. 2001 Apr; 6(4): 390-7. PMID: 11372197, ISSN: 0949-8257.

Strop P, Smith KS, Iverson TM, Ferry JG, Rees DC. Crystal structure of the "cab"-type beta class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicum. J. Biol. Chem [print-electronic]. 2001 Mar 3/30/2001; 276(13): 10299-305. PMID: 11096105, PII: M009182200, DOI: 10.1074/jbc.M009182200, ISSN: 0021-9258.

Iverson TM, Alber BE, Kisker C, Ferry JG, Rees DC. A closer look at the active site of gamma-class carbonic anhydrases: high-resolution crystallographic studies of the carbonic anhydrase from Methanosarcina thermophila. Biochemistry. 2000 Aug 8/8/2000; 39(31): 9222-31. PMID: 10924115, PII: bi000204s, ISSN: 0006-2960.

Iverson TM, Luna-Chavez C, Schröder I, Cecchini G, Rees DC. Analyzing your complexes: structure of the quinol-fumarate reductase respiratory complex. Curr. Opin. Struct. Biol. 2000 Aug; 10(4): 448-55. PMID: 10981634, PII: S0959-440X(00)00113-5, ISSN: 0959-440X.

Luna-Chavez C, Iverson TM, Rees DC, Cecchini G. Overexpression, purification, and crystallization of the membrane-bound fumarate reductase from Escherichia coli. Protein Expr. Purif. 2000 Jun; 19(1): 188-96. PMID: 10833406, PII: S1046-5928(00)91238-5, DOI: 10.1006/prep.2000.1238, ISSN: 1046-5928.

Iverson TM, Luna-Chavez C, Cecchini G, Rees DC. Structure of the Escherichia coli fumarate reductase respiratory complex. Science. 1999 Jun 6/18/1999; 284(5422): 1961-6. PMID: 10373108, PII: 7600, ISSN: 0036-8075.

Iverson TM, Arciero DM, Hsu BT, Logan MS, Hooper AB, Rees DC. Heme packing motifs revealed by the crystal structure of the tetra-heme cytochrome c554 from Nitrosomonas europaea. Nat. Struct. Biol. 1998 Nov; 5(11): 1005-12. PMID: 9808046, DOI: 10.1038/2975, ISSN: 1072-8368.