Kevin P. M. Currie, Ph.D.

Associate Professor
Faculty Appointments
Associate Professor of Anesthesiology Associate Professor of Pharmacology
Ph.D., Physiology, London University, London, United KingdomB.Sc., Pharmacology, University of Edinburgh, Edinburgh, United Kingdom
Office Address
Department of Anesthesiology 1161 21st Ave. S T-4202 MCN
Nashville, TN 37232-2520
Research Description
Research in the Currie lab is directed toward understanding the function and regulation of voltage-gated calcium channels and calcium-dependent exocytosis, the fundamental cellular mechanism that underlies synaptic transmission and neuroendocrine hormone release. One current focus is the release of catecholamines and other neuroendocrine hormones from adrenal chromaffin cells, central mediators of the sympatho-adrenal stress response (the fight-or-flight response). Chromaffin cells are also a widely used model that enables detailed analyses of stimulus-secretion coupling. G protein coupled receptors (GPCRs) orchestrate precise autocrine / paracrine control of calcium entry and secretion. We are investigating the GPCRs and downstream pathways that are recruited by inflammatory mediators and other paracrine or systemic signals to control transmitter release. In another project (in collaboration with Randy Blakely) we are investigating novel roles for the serotonin transporter (SERT), an important target for antidepressants, in controlling catecholamine secretion. We are also interested in glial regulation of dorsal root ganglion neurons and how this relates to itch / pain signaling (in collaboration with Bruce Carter). We use wild-type and transgenic mice along with a combination of techniques including patch-clamp electrophysiology, carbon fiber amperometry, and fluorescent calcium imaging. Our overall goal is to understand the regulation of calcium channels and transmitter release under physiological conditions, and identify potential therapeutic targets for treatment of nervous and endocrine system disorders in which these finely tuned processes are disrupted
Research Keywords
calcium signaling, ion channels, neurotransmitter release, exocytosis, synaptic function, G-protein, electrophysiology, patch clamp, amperometry, neurophysiology, signal transduction
Currie KP. Focus on "Activation of MEK/ERK signaling contributes to the PACAP-induced increase in guinea pig cardiac neuron excitability". Am. J. Physiol., Cell Physiol [print-electronic]. 2016 Sep 9/21/2016; ajpcell.00270.2016. PMID: 27653986, PII: ajpcell.00270.2016, DOI: 10.1152/ajpcell.00270.2016, ISSN: 1522-1563.

Brindley RL, Bauer MB, Blakely RD, Currie KP. An interplay between the serotonin transporter (SERT) and 5-HT receptors controls stimulus-secretion coupling in sympathoadrenal chromaffin cells. Neuropharmacology [print-electronic]. 2016 Aug 8/17/2016; PMID: 27544824, PII: S0028-3908(16)30350-1, DOI: 10.1016/j.neuropharm.2016.08.015, ISSN: 1873-7064.

Zhu L, McDavid S, Currie KP. "Slow" Voltage-Dependent Inactivation of CaV2.2 Calcium Channels Is Modulated by the PKC Activator Phorbol 12-Myristate 13-Acetate (PMA). PLoS ONE. 2015; 10(7): e0134117. PMID: 26222492, PMCID: PMC4519294, PII: PONE-D-15-18856, DOI: 10.1371/journal.pone.0134117, ISSN: 1932-6203.

McDavid S, Bauer MB, Brindley RL, Jewell ML, Currie KP. Butanol isomers exert distinct effects on voltage-gated calcium channel currents and thus catecholamine secretion in adrenal chromaffin cells. PLoS ONE. 2014; 9(10): e109203. PMID: 25275439, PMCID: PMC4183593, PII: PONE-D-14-30134, DOI: 10.1371/journal.pone.0109203, ISSN: 1932-6203.

Ges IA, Brindley RL, Currie KP, Baudenbacher FJ. A microfluidic platform for chemical stimulation and real time analysis of catecholamine secretion from neuroendocrine cells. Lab Chip. 2013 Dec 12/7/2013; 13(23): 4663-73. PMID: 24126415, PMCID: PMC3892771, DOI: 10.1039/c3lc50779c, ISSN: 1473-0189.

Zamponi GW, Currie KP. Regulation of Ca(V)2 calcium channels by G protein coupled receptors. Biochim. Biophys. Acta [print-electronic]. 2013 Jul; 1828(7): 1629-43. PMID: 23063655, PMCID: PMC3556207, PII: S0005-2736(12)00353-7, DOI: 10.1016/j.bbamem.2012.10.004, ISSN: 0006-3002.

Todd RD, McDavid SM, Brindley RL, Jewell ML, Currie KP. Gabapentin inhibits catecholamine release from adrenal chromaffin cells. Anesthesiology. 2012 May; 116(5): 1013-24. PMID: 22417967, PMCID: PMC3341086, DOI: 10.1097/ALN.0b013e31825153ea, ISSN: 1528-1175.

Ges IA, Currie KP, Baudenbacher F. Electrochemical detection of catecholamine release using planar iridium oxide electrodes in nanoliter microfluidic cell culture volumes. Biosens Bioelectron [print-electronic]. 2012 Apr 4/15/2012; 34(1): 30-6. PMID: 22398270, PMCID: PMC3793634, PII: S0956-5663(11)00794-9, DOI: 10.1016/j.bios.2011.11.050, ISSN: 1873-4235.

Jewell ML, Breyer RM, Currie KP. Regulation of calcium channels and exocytosis in mouse adrenal chromaffin cells by prostaglandin EP3 receptors. Mol. Pharmacol [print-electronic]. 2011 Jun; 79(6): 987-96. PMID: 21383044, PMCID: PMC3102550, PII: mol.110.068569, DOI: 10.1124/mol.110.068569, ISSN: 1521-0111.

Currie KP. G protein modulation of CaV2 voltage-gated calcium channels. Channels (Austin) [print-electronic]. 2010 Nov; 4(6): 497-509. PMID: 21150298, PMCID: PMC3052249, PII: 12871, ISSN: 1933-6969.

Currie KP. Inhibition of Ca2+ channels and adrenal catecholamine release by G protein coupled receptors. Cell. Mol. Neurobiol. 2010 Nov; 30(8): 1201-8. PMID: 21061161, PMCID: PMC3028936, DOI: 10.1007/s10571-010-9596-7, ISSN: 1573-6830.

Yoon EJ, Hamm HE, Currie KP. G protein betagamma subunits modulate the number and nature of exocytotic fusion events in adrenal chromaffin cells independent of calcium entry. J. Neurophysiol [print-electronic]. 2008 Nov; 100(5): 2929-39. PMID: 18815342, PMCID: PMC2585407, PII: 90839.2008, DOI: 10.1152/jn.90839.2008, ISSN: 0022-3077.

Fox AP, Cahill AL, Currie KP, Grabner C, Harkins AB, Herring B, Hurley JH, Xie Z. N- and P/Q-type Ca2+ channels in adrenal chromaffin cells. Acta Physiol (Oxf) [print-electronic]. 2008 Feb; 192(2): 247-61. PMID: 18021320, PII: APS1817, DOI: 10.1111/j.1748-1716.2007.01817.x, ISSN: 1748-1716.

McDavid S, Currie KP. G-proteins modulate cumulative inactivation of N-type (Cav2.2) calcium channels. J. Neurosci. 2006 Dec 12/20/2006; 26(51): 13373-83. PMID: 17182788, PII: 26/51/13373, DOI: 10.1523/JNEUROSCI.3332-06.2006, ISSN: 1529-2401.

Dzhura EV, He W, Currie KP. Linopirdine modulates calcium signaling and stimulus-secretion coupling in adrenal chromaffin cells by targeting M-type K+ channels and nicotinic acetylcholine receptors. J. Pharmacol. Exp. Ther [print-electronic]. 2006 Mar; 316(3): 1165-74. PMID: 16280412, PII: jpet.105.095570, DOI: 10.1124/jpet.105.095570, ISSN: 0022-3565.

Xie Z, Currie KP, Fox AP. Etomidate elevates intracellular calcium levels and promotes catecholamine secretion in bovine chromaffin cells. J. Physiol. (Lond.) [print-electronic]. 2004 Nov 11/1/2004; 560(Pt 3): 677-90. PMID: 15331676, PMCID: PMC1665276, PII: jphysiol.2004.070664, DOI: 10.1113/jphysiol.2004.070664, ISSN: 0022-3751.

Xie Z, Currie KP, Cahill AL, Fox AP. Role of Cl- co-transporters in the excitation produced by GABAA receptors in juvenile bovine adrenal chromaffin cells. J. Neurophysiol [print-electronic]. 2003 Dec; 90(6): 3828-37. PMID: 12968012, PII: 00617.2003, DOI: 10.1152/jn.00617.2003, ISSN: 0022-3077.

Zhou YP, Sreenan S, Pan CY, Currie KP, Bindokas VP, Horikawa Y, Lee JP, Ostrega D, Ahmed N, Baldwin AC, Cox NJ, Fox AP, Miller RJ, Bell GI, Polonsky KS. A 48-hour exposure of pancreatic islets to calpain inhibitors impairs mitochondrial fuel metabolism and the exocytosis of insulin. Metab. Clin. Exp. 2003 May; 52(5): 528-34. PMID: 12759879, PII: S0026049503000040, DOI: 10.1053/meta.2003.50091, ISSN: 0026-0495.

Currie KP, Fox AP. Cause for excite-M-ent in adrenal chromaffin cells. J. Physiol. (Lond.). 2002 May 5/1/2002; 540(Pt 3): 729. PMID: 11986363, PMCID: PMC2290266, PII: PHY_19653, ISSN: 0022-3751.

Currie KP, Fox AP. Differential facilitation of N- and P/Q-type calcium channels during trains of action potential-like waveforms. J. Physiol. (Lond.). 2002 Mar 3/1/2002; 539(Pt 2): 419-31. PMID: 11882675, PMCID: PMC2290166, PII: PHY_13206, ISSN: 0022-3751.

Sreenan SK, Zhou YP, Otani K, Hansen PA, Currie KP, Pan CY, Lee JP, Ostrega DM, Pugh W, Horikawa Y, Cox NJ, Hanis CL, Burant CF, Fox AP, Bell GI, Polonsky KS. Calpains play a role in insulin secretion and action. Diabetes. 2001 Sep; 50(9): 2013-20. PMID: 11522666, ISSN: 0012-1797.

Hurley JH, Cahill AL, Currie KP, Fox AP. The role of dynamic palmitoylation in Ca2+ channel inactivation. Proc. Natl. Acad. Sci. U.S.A. 2000 Aug 8/1/2000; 97(16): 9293-8. PMID: 10900273, PMCID: PMC16861, PII: 160589697, DOI: 10.1073/pnas.160589697, ISSN: 0027-8424.

Currie KP, Fox AP. Voltage-dependent, pertussis toxin insensitive inhibition of calcium currents by histamine in bovine adrenal chromaffin cells. J. Neurophysiol. 2000 Mar; 83(3): 1435-42. PMID: 10712470, ISSN: 0022-3077.

Currie KP, Zhou Z, Fox AP. Evidence for paracrine signaling between macrophages and bovine adrenal chromaffin cell Ca(2+) channels. J. Neurophysiol. 2000 Jan; 83(1): 280-7. PMID: 10634871, ISSN: 0022-3077.

Currie KP, Fox AP. Comparison of N- and P/Q-type voltage-gated calcium channel current inhibition. J. Neurosci. 1997 Jun 6/15/1997; 17(12): 4570-9. PMID: 9169518, ISSN: 0270-6474.

Currie KP, Fox AP. ATP serves as a negative feedback inhibitor of voltage-gated Ca2+ channel currents in cultured bovine adrenal chromaffin cells. Neuron. 1996 May; 16(5): 1027-36. PMID: 8630241, PII: S0896-6273(00)80126-9, ISSN: 0896-6273.

Scott RH, Sutton KG, Griffin A, Stapleton SR, Currie KP. Aspects of calcium-activated chloride currents: a neuronal perspective. Pharmacol. Ther. 1995 Jun; 66(3): 535-65. PMID: 7494858, PII: 0163-7258(95)00018-C, ISSN: 0163-7258.

Currie KP, Wootton JF, Scott RH. Activation of Ca(2+)-dependent Cl- currents in cultured rat sensory neurones by flash photolysis of DM-nitrophen. J. Physiol. (Lond.). 1995 Jan 1/15/1995; 482 ( Pt 2): 291-307. PMID: 7714823, PMCID: PMC1157729, ISSN: 0022-3751.

Stapleton SR, Currie KP, Scott RH, Bell BA. Palmitoyl-DL-carnitine has calcium-dependent effects on cultured neurones from rat dorsal root ganglia. Br. J. Pharmacol. 1992 Dec; 107(4): 1192-7. PMID: 1334752, PMCID: PMC1907908, ISSN: 0007-1188.

Currie KP, Swann K, Galione A, Scott RH. Activation of Ca(2+)-dependent currents in cultured rat dorsal root ganglion neurones by a sperm factor and cyclic ADP-ribose. Mol. Biol. Cell. 1992 Dec; 3(12): 1415-25. PMID: 1283541, PMCID: PMC275709, ISSN: 1059-1524.

Currie KP, Scott RH. Calcium-activated currents in cultured neurones from rat dorsal root ganglia. Br. J. Pharmacol. 1992 Jul; 106(3): 593-602. PMID: 1324075, PMCID: PMC1907550, ISSN: 0007-1188.

Scott RH, Currie KP, Sutton KG, Dolphin AC. Modulation of neuronal Ca(2+)-dependent currents by neurotransmitters, G-proteins and toxins. Biochem. Soc. Trans. 1992 May; 20(2): 443-9. PMID: 1383061, ISSN: 0300-5127.