P. Anthony Weil, Ph.D.



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Faculty Appointments
Professor of Molecular Physiology and Biophysics
Ph.D., Biochemistry, University of Texas Health Science Center, Houston, TexasB.S., Biology, Northern Illinois University, Dekalb, Illinois
Office Address
746A Robinson Research Building
21st and Garland
Nashville, TN 37232-0615
Research Description
The focus of research in our laboratory is to understand the molecular mechanisms of eukaryotic transcription initiation. For the last ten or so years we have been examining the eukaryotic transcription factors which mediate initiation complex formation and thus represent potential targets for trans-regulation. We have utilized the simple eukaryote, Saccharomyces cerevisiae or Baker's Yeast, for our work. This organism was chosen for our studies because both biochemical and genetic approaches can be taken with yeasts. In our experiments we study the factors required for transcription initiation by RNA polymerase II (RNAP II). RNAP II transcribes the genes which encode mRNAs. We have developed methods for the solubilization, characterization and purification of the complete complement of RNAP II-specific factors, and our current focus is on one of these factors the multisubunit factor termed TFIID. All of the proteins which comprise TFIID have very interesting biochemical properties. One of the factors, known as TBP, or TATA box Binding Protein, is a sequence specific DNA binding protein which interacts with the ubiquitous TATA box promoter element. while others are not. The other subunits of TFIID presumably interact with other promoter elements, RNAPII, positive-acting transcription factors or other general transcription factors such as TFIIA, TFIIB, TFIIE, TFIIF or TFIIF. <p>
Our immediate efforts have been expended towards cloning the yeast genes which encode these genes encoding the TFIID subunits. We have been successful in cloning the genes which encode the multiple (15 distinct genes) subunits of yeast TFIID. Our interest in cloning these genes are several and are summarized here as are the types of studies which will be the focus of our research in the future--each could comprise a student rotation project: 1) The cloned genes give us the wherewithal to overexpress the corresponding gene products. Purified factors prepared from the cloned genes will be used for in vitro mechanistic studies. 2) Using the cloned genes we are examining the structure-function relationships of these important molecules. 3) We are dissecting the genetic control elements which regulate expression of the transcription factor genes themselves. These studies are being performed with an eye towards understanding global control of macromolecular biosynthesis. 4) Finally, we are using the cloned genes, in conditionally lethal forms, to identify via suppressor analyses genes whose products interact with these multi-functional general transcription initiation factors. Specific examples of the types of studies and the results which we have obtained are listed in "Selected Publications."
Research Keywords
Molecular mechanisms of transcriptional regulation
Layer JH, Weil PA. Direct TFIIA-TFIID protein contacts drive budding yeast ribosomal protein gene transcription. J. Biol. Chem [print-electronic]. 2013 Aug 8/9/2013; 288(32): 23273-94. PMID: 23814059, PMCID: PMC3743499, PII: M113.486829, DOI: 10.1074/jbc.M113.486829, ISSN: 1083-351X.

Jian L, Niu X, Xia Z, Samir P, Sumanasekera C, Mu Z, Jennings JL, Hoek KL, Allos T, Howard LM, Edwards KM, Weil PA, Link AJ. A novel algorithm for validating peptide identification from a shotgun proteomics search engine. J. Proteome Res [print-electronic]. 2013 Mar 3/1/2013; 12(3): 1108-19. PMID: 23402659, PMCID: PMC3608465, DOI: 10.1021/pr300631t, ISSN: 1535-3907.

Layer JH, Miller SG, Weil PA. Direct transactivator-transcription factor IID (TFIID) contacts drive yeast ribosomal protein gene transcription. J. Biol. Chem [print-electronic]. 2010 May 5/14/2010; 285(20): 15489-99. PMID: 20189987, PMCID: PMC2865315, PII: M110.104810, DOI: 10.1074/jbc.M110.104810, ISSN: 1083-351X.

Layer JH, Weil PA. Ubiquitous antisense transcription in eukaryotes: novel regulatory mechanism or byproduct of opportunistic RNA polymerase?. F1000 Biol Rep. 2009; 1: 33. PMID: 20948652, PMCID: PMC2924692, DOI: 10.3410/B1-33, ISSN: 1757-594X.

Arnett DR, Jennings JL, Tabb DL, Link AJ, Weil PA. A proteomics analysis of yeast Mot1p protein-protein associations: insights into mechanism. Mol. Cell Proteomics [print-electronic]. 2008 Nov; 7(11): 2090-106. PMID: 18596064, PMCID: PMC2577210, PII: M800221-MCP200, DOI: 10.1074/mcp.M800221-MCP200, ISSN: 1535-9484.

Bendjennat M, Weil PA. The transcriptional repressor activator protein Rap1p is a direct regulator of TATA-binding protein. J. Biol. Chem [print-electronic]. 2008 Mar 3/28/2008; 283(13): 8699-710. PMID: 18195009, PMCID: PMC2417159, PII: M709436200, DOI: 10.1074/jbc.M709436200, ISSN: 0021-9258.

Garbett KA, Tripathi MK, Cencki B, Layer JH, Weil PA. Yeast TFIID serves as a coactivator for Rap1p by direct protein-protein interaction. Mol. Cell. Biol [print-electronic]. 2007 Jan; 27(1): 297-311. PMID: 17074814, PMCID: PMC1800639, PII: MCB.01558-06, DOI: 10.1128/MCB.01558-06, ISSN: 0270-7306.

Powell DW, Weaver CM, Jennings JL, McAfee KJ, He Y, Weil PA, Link AJ. Cluster analysis of mass spectrometry data reveals a novel component of SAGA. Mol. Cell. Biol. 2004 Aug; 24(16): 7249-59. PMID: 15282323, PMCID: PMC479721, PII: 24/16/7249, DOI: 10.1128/MCB.24.16.7249-7259.2004, ISSN: 0270-7306.

Singh MV, Bland CE, Weil PA. Molecular and genetic characterization of a Taf1p domain essential for yeast TFIID assembly. Mol. Cell. Biol. 2004 Jun; 24(11): 4929-42. PMID: 15143185, PMCID: PMC416396, PII: 24/11/4929, DOI: 10.1128/MCB.24.11.4929-4942.2004, ISSN: 0270-7306.

Leurent C, Sanders SL, Demény MA, Garbett KA, Ruhlmann C, Weil PA, Tora L, Schultz P. Mapping key functional sites within yeast TFIID. EMBO J [print-electronic]. 2004 Feb 2/25/2004; 23(4): 719-27. PMID: 14765106, PMCID: PMC381015, PII: 7600111, DOI: 10.1038/sj.emboj.7600111, ISSN: 0261-4189.

Gumbs OH, Campbell AM, Weil PA. High-affinity DNA binding by a Mot1p-TBP complex: implications for TAF-independent transcription. EMBO J. 2003 Jun 6/16/2003; 22(12): 3131-41. PMID: 12805227, PMCID: PMC162156, DOI: 10.1093/emboj/cdg304, ISSN: 0261-4189.

Klein J, Nolden M, Sanders SL, Kirchner J, Weil PA, Melcher K. Use of a genetically introduced cross-linker to identify interaction sites of acidic activators within native transcription factor IID and SAGA. J. Biol. Chem [print-electronic]. 2003 Feb 2/28/2003; 278(9): 6779-86. PMID: 12501245, PII: M212514200, DOI: 10.1074/jbc.M212514200, ISSN: 0021-9258.

Thuault S, Gangloff YG, Kirchner J, Sanders S, Werten S, Romier C, Weil PA, Davidson I. Functional analysis of the TFIID-specific yeast TAF4 (yTAF(II)48) reveals an unexpected organization of its histone-fold domain. J. Biol. Chem [print-electronic]. 2002 Nov 11/22/2002; 277(47): 45510-7. PMID: 12237303, PII: M206556200, DOI: 10.1074/jbc.M206556200, ISSN: 0021-9258.

Andrau JC, Van Oevelen CJ, Van Teeffelen HA, Weil PA, Holstege FC, Timmers HT. Mot1p is essential for TBP recruitment to selected promoters during in vivo gene activation. EMBO J. 2002 Oct 10/1/2002; 21(19): 5173-83. PMID: 12356733, PMCID: PMC129025, ISSN: 0261-4189.

Sanders SL, Garbett KA, Weil PA. Molecular characterization of Saccharomyces cerevisiae TFIID. Mol. Cell. Biol. 2002 Aug; 22(16): 6000-13. PMID: 12138208, PMCID: PMC133964, ISSN: 0270-7306.

Singh MV, Weil PA. A method for plasmid purification directly from yeast. Anal. Biochem. 2002 Aug 8/1/2002; 307(1): 13-7. PMID: 12137773, PII: S0003269702000180, ISSN: 0003-2697.

Sanders SL, Jennings J, Canutescu A, Link AJ, Weil PA. Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry. Mol. Cell. Biol. 2002 Jul; 22(13): 4723-38. PMID: 12052880, PMCID: PMC133885, ISSN: 0270-7306.

Leurent C, Sanders S, Ruhlmann C, Mallouh V, Weil PA, Kirschner DB, Tora L, Schultz P. Mapping histone fold TAFs within yeast TFIID. EMBO J. 2002 Jul 7/1/2002; 21(13): 3424-33. PMID: 12093743, PMCID: PMC126091, DOI: 10.1093/emboj/cdf342, ISSN: 0261-4189.

Kirschner DB, vom Baur E, Thibault C, Sanders SL, Gangloff YG, Davidson I, Weil PA, Tora L. Distinct mutations in yeast TAF(II)25 differentially affect the composition of TFIID and SAGA complexes as well as global gene expression patterns. Mol. Cell. Biol. 2002 May; 22(9): 3178-93. PMID: 11940675, PMCID: PMC133751, ISSN: 0270-7306.

Banik U, Beechem JM, Klebanow E, Schroeder S, Weil PA. Fluorescence-based analyses of the effects of full-length recombinant TAF130p on the interaction of TATA box-binding protein with TATA box DNA. J. Biol. Chem [print-electronic]. 2001 Dec 12/28/2001; 276(52): 49100-9. PMID: 11677244, PII: M109246200, DOI: 10.1074/jbc.M109246200, ISSN: 0021-9258.

Kirchner J, Sanders SL, Klebanow E, Weil PA. Molecular genetic dissection of TAF25, an essential yeast gene encoding a subunit shared by TFIID and SAGA multiprotein transcription factors. Mol. Cell. Biol. 2001 Oct; 21(19): 6668-80. PMID: 11533254, PMCID: PMC99812, ISSN: 0270-7306.

Gangloff YG, Sanders SL, Romier C, Kirschner D, Weil PA, Tora L, Davidson I. Histone folds mediate selective heterodimerization of yeast TAF(II)25 with TFIID components yTAF(II)47 and yTAF(II)65 and with SAGA component ySPT7. Mol. Cell. Biol. 2001 Mar; 21(5): 1841-53. PMID: 11238921, PMCID: PMC86751, DOI: 10.1128/MCB.21.5.1841-1853.2001, ISSN: 0270-7306.

Sanders SL, Weil PA. Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex. J. Biol. Chem. 2000 May 5/5/2000; 275(18): 13895-900. PMID: 10788514, PII: 275/18/13895, ISSN: 0021-9258.

Sanders SL, Klebanow ER, Weil PA. TAF25p, a non-histone-like subunit of TFIID and SAGA complexes, is essential for total mRNA gene transcription in vivo. J. Biol. Chem. 1999 Jul 7/2/1999; 274(27): 18847-50. PMID: 10383379, ISSN: 0021-9258.

Klebanow ER, Weil PA. A rapid technique for the determination of unknown plasmid library insert DNA sequence directly from intact yeast cells. Yeast. 1999 Apr; 15(6): 527-31. PMID: 10234790, PII: 10.1002/(SICI)1097-0061(199904)15:6<527::AID-YEA374>3.0.CO;2-0, DOI: 10.1002/(SICI)1097-0061(199904)15:6<527::AID-YEA374>3.0.CO;2-0, ISSN: 0749-503X.

Muldrow TA, Campbell AM, Weil PA, Auble DT. MOT1 can activate basal transcription in vitro by regulating the distribution of TATA binding protein between promoter and nonpromoter sites. Mol. Cell. Biol. 1999 Apr; 19(4): 2835-45. PMID: 10082549, PMCID: PMC84076, ISSN: 0270-7306.

Reuter, T.A., Campbell, A.M., Weil P.A. and Auble, D.T. Mot1p Can Activate Basal Transcription In Vitro by Regulating the Distribution of TBP Between Promoter and Nonpromoter Sites. 1999.

1999; Available from: .

Komarnitsky PB, Klebanow ER, Weil PA, Denis CL. ADR1-mediated transcriptional activation requires the presence of an intact TFIID complex. Mol. Cell. Biol. 1998 Oct; 18(10): 5861-7. PMID: 9742103, PMCID: PMC109172, ISSN: 0270-7306.

Schroeder SC, Weil PA. Biochemical and genetic characterization of the dominant positive element driving transcription ofthe yeast TBP-encoding gene, SPT15. Nucleic Acids Res. 1998 Sep 9/15/1998; 26(18): 4186-95. PMID: 9722639, PMCID: PMC147844, PII: gkb683, ISSN: 0305-1048.

Schroeder SC, Weil PA. Genetic tests of the role of Abf1p in driving transcription of the yeast TATA box bindng protein-encoding gene, SPT15. J. Biol. Chem. 1998 Jul 7/31/1998; 273(31): 19884-91. PMID: 9677425, ISSN: 0021-9258.

Patterson GH, Schroeder SC, Bai Y, Weil A, Piston DW. Quantitative imaging of TATA-binding protein in living yeast cells. Yeast. 1998 Jun 6/30/1998; 14(9): 813-25. PMID: 9818719, PII: 10.1002/(SICI)1097-0061(19980630)14:9<813::AID-YEA280>3.0.CO;2-2, DOI: 10.1002/(SICI)1097-0061(19980630)14:9<813::AID-YEA280>3.0.CO;2-2, ISSN: 0749-503X.

Drysdale CM, Jackson BM, McVeigh R, Klebanow ER, Bai Y, Kokubo T, Swanson M, Nakatani Y, Weil PA, Hinnebusch AG. The Gcn4p activation domain interacts specifically in vitro with RNA polymerase II holoenzyme, TFIID, and the Adap-Gcn5p coactivator complex. Mol. Cell. Biol. 1998 Mar; 18(3): 1711-24. PMID: 9488488, PMCID: PMC108886, ISSN: 0270-7306.

Patterson, G.H., Schroeder, S.C., Bai, Y., P. A. Weil and David W. Piston. Quantitative Imaging of Green Fluorescent Protein-TBP fusion Proteins: Asymmetric Distribution of TBP between Yeast Mothers and Daughters. 1998.

Schroeder, S. and Weil, P.A. Genetic Tests of the Role of Abf1p in Driving Transcription of the Yeast TBP- encoding Gene. 1998.

1998; Available from: .

Bai Y, Perez GM, Beechem JM, Weil PA. Structure-function analysis of TAF130: identification and characterization of a high-affinity TATA-binding protein interaction domain in the N terminus of yeast TAF(II)130. Mol. Cell. Biol. 1997 Jun; 17(6): 3081-93. PMID: 9154807, PMCID: PMC232161, ISSN: 0270-7306.

Klebanow ER, Poon D, Zhou S, Weil PA. Cloning and characterization of an essential Saccharomyces cerevisiae gene, TAF40, which encodes yTAFII40, an RNA polymerase II-specific TATA-binding protein-associated factor. J. Biol. Chem. 1997 Apr 4/4/1997; 272(14): 9436-42. PMID: 9083082, ISSN: 0021-9258.

Yamamoto T, Poon D, Weil PA, Horikoshi M. Molecular genetic elucidation of the tripartite structure of the Schizosaccharomyces pombe 72 kDa TFIID subunit which contains a WD40 structural motif. Genes Cells. 1997 Apr; 2(4): 245-54. PMID: 9224658, ISSN: 1356-9597.

Bai, Y. , Perez, G.M., Beechem, J.M. and Weil, P.A. Structure-Function Analysis of TAF130: Identification and Characterization of a High Affinity TBP Interaction Domain in the N-terminus of yTAFII130. 1997.

Moqtaderi Z, Bai Y, Poon D, Weil PA, Struhl K. TBP-associated factors are not generally required for transcriptional activation in yeast. Nature. 1996 Sep 9/12/1996; 383(6596): 188-91. PMID: 8774887, DOI: 10.1038/383188a0, ISSN: 0028-0836.

Klebanow ER, Poon D, Zhou S, Weil PA. Isolation and characterization of TAF25, an essential yeast gene that encodes an RNA polymerase II-specific TATA-binding protein-associated factor. J. Biol. Chem. 1996 Jun 6/7/1996; 271(23): 13706-15. PMID: 8662725, ISSN: 0021-9258.

Moqtaderi, Z., Bai, Y., Poon, D., Weil, P.A. and Struhl, K. TBP-associated factors are not generally required for transcriptional activation in yeast. 1996.

Poon D, Bai Y, Campbell AM, Bjorklund S, Kim YJ, Zhou S, Kornberg RD, Weil PA. Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 1995 Aug 8/29/1995; 92(18): 8224-8. PMID: 7667272, PMCID: PMC41129, ISSN: 0027-8424.

Perez-Howard GM, Weil PA, Beechem JM. Yeast TATA binding protein interaction with DNA: fluorescence determination of oligomeric state, equilibrium binding, on-rate, and dissociation kinetics. Biochemistry. 1995 Jun 6/27/1995; 34(25): 8005-17. PMID: 7794913, PMCID: PMC2891535, ISSN: 0006-2960.

Henry NL, Campbell AM, Feaver WJ, Poon D, Weil PA, Kornberg RD. TFIIF-TAF-RNA polymerase II connection. Genes Dev. 1994 Dec 12/1/1994; 8(23): 2868-78. PMID: 7995524, ISSN: 0890-9369.

Schroeder SC, Wang CK, Weil PA. Identification of the cis-acting DNA sequence elements regulating the transcription of the Saccharomyces cerevisiae gene encoding TBP, the TATA box binding protein. J. Biol. Chem. 1994 Nov 11/11/1994; 269(45): 28335-46. PMID: 7961772, ISSN: 0021-9258.

Robinson GL, Cordle SR, Henderson E, Weil PA, Teitelman G, Stein R. Isolation and characterization of a novel transcription factor that binds to and activates insulin control element-mediated expression. Mol. Cell. Biol. 1994 Oct; 14(10): 6704-14. PMID: 7935390, PMCID: PMC359201, ISSN: 0270-7306.

Poon D, Campbell AM, Bai Y, Weil PA. Yeast Taf170 is encoded by MOT1 and exists in a TATA box-binding protein (TBP)-TBP-associated factor complex distinct from transcription factor IID. J. Biol. Chem. 1994 Sep 9/16/1994; 269(37): 23135-40. PMID: 8083216, ISSN: 0021-9258.

Leibham D, Wong MW, Cheng TC, Schroeder S, Weil PA, Olson EN, Perry M. Binding of TFIID and MEF2 to the TATA element activates transcription of the Xenopus MyoDa promoter. Mol. Cell. Biol. 1994 Jan; 14(1): 686-99. PMID: 8264638, PMCID: PMC358418, ISSN: 0270-7306.

Poon D, Weil PA. Immunopurification of yeast TATA-binding protein and associated factors. Presence of transcription factor IIIB transcriptional activity. J. Biol. Chem. 1993 Jul 7/25/1993; 268(21): 15325-8. PMID: 8340360, ISSN: 0021-9258.

Poon D, Knittle RA, Sabelko KA, Yamamoto T, Horikoshi M, Roeder RG, Weil PA. Genetic and biochemical analyses of yeast TATA-binding protein mutants. J. Biol. Chem. 1993 Mar 3/5/1993; 268(7): 5005-13. PMID: 8444878, ISSN: 0021-9258.

Yamamoto T, Horikoshi M, Wang J, Hasegawa S, Weil PA, Roeder RG. A bipartite DNA binding domain composed of direct repeats in the TATA box binding factor TFIID. Proc. Natl. Acad. Sci. U.S.A. 1992 Apr 4/1/1992; 89(7): 2844-8. PMID: 1557391, PMCID: PMC48759, ISSN: 0027-8424.

Parsons MC, Weil PA. Cloning of TFC1, the Saccharomyces cerevisiae gene encoding the 95-kDa subunit of transcription factor TFIIIC. J. Biol. Chem. 1992 Feb 2/15/1992; 267(5): 2894-901. PMID: 1737746, ISSN: 0021-9258.

Poon D, Schroeder S, Wang CK, Yamamoto T, Horikoshi M, Roeder RG, Weil PA. The conserved carboxy-terminal domain of Saccharomyces cerevisiae TFIID is sufficient to support normal cell growth. Mol. Cell. Biol. 1991 Oct; 11(10): 4809-21. PMID: 1922021, PMCID: PMC361446, ISSN: 0270-7306.

Cordle SR, Whelan J, Henderson E, Masuoka H, Weil PA, Stein R. Insulin gene expression in nonexpressing cells appears to be regulated by multiple distinct negative-acting control elements. Mol. Cell. Biol. 1991 May; 11(5): 2881-6. PMID: 2017182, PMCID: PMC360077, ISSN: 0270-7306.

Cordle SR, Henderson E, Masuoka H, Weil PA, Stein R. Pancreatic beta-cell-type-specific transcription of the insulin gene is mediated by basic helix-loop-helix DNA-binding proteins. Mol. Cell. Biol. 1991 Mar; 11(3): 1734-8. PMID: 1996119, PMCID: PMC369485, ISSN: 0270-7306.

Hoffmann A, Horikoshi M, Wang CK, Schroeder S, Weil PA, Roeder RG. Cloning of the Schizosaccharomyces pombe TFIID gene reveals a strong conservation of functional domains present in Saccharomyces cerevisiae TFIID. Genes Dev. 1990 Jul; 4(7): 1141-8. PMID: 2210373, ISSN: 0890-9369.

Horikoshi M, Yamamoto T, Ohkuma Y, Weil PA, Roeder RG. Analysis of structure-function relationships of yeast TATA box binding factor TFIID. Cell. 1990 Jun 6/29/1990; 61(7): 1171-8. PMID: 2194665, PII: 0092-8674(90)90681-4, ISSN: 0092-8674.

Felts SJ, Weil PA, Chalkley R. Transcription factor requirements for in vitro formation of transcriptionally competent 5S rRNA gene chromatin. Mol. Cell. Biol. 1990 May; 10(5): 2390-401. PMID: 2183033, PMCID: PMC360587, ISSN: 0270-7306.

Karnitz L, Poon D, Weil PA, Chalkley R. Identification and purification of a yeast transcriptional trans-activator. The yeast homolog of the Rous sarcoma virus internal enhancer binding factor. J. Biol. Chem. 1990 Apr 4/15/1990; 265(11): 6131-8. PMID: 2156843, ISSN: 0021-9258.

Whelan J, Cordle SR, Henderson E, Weil PA, Stein R. Identification of a pancreatic beta-cell insulin gene transcription factor that binds to and appears to activate cell-type-specific expression: its possible relationship to other cellular factors that bind to a common insulin gene sequence. Mol. Cell. Biol. 1990 Apr; 10(4): 1564-72. PMID: 2181278, PMCID: PMC362261, ISSN: 0270-7306.

Parsons MC, Weil PA. Purification and characterization of Saccharomyces cerevisiae transcription factor TFIIIC. Polypeptide composition defined with polyclonal antibodies. J. Biol. Chem. 1990 Mar 3/25/1990; 265(9): 5095-103. PMID: 2180956, ISSN: 0021-9258.

Nichols M, Bell J, Klekamp MS, Weil PA, Söll D. Multiple mutations of the first gene of a dimeric tRNA gene abolish in vitro tRNA gene transcription. J. Biol. Chem. 1989 Oct 10/15/1989; 264(29): 17084-90. PMID: 2676999, ISSN: 0021-9258.

Horikoshi M, Wang CK, Fujii H, Cromlish JA, Weil PA, Roeder RG. Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box. Nature. 1989 Sep 9/28/1989; 341(6240): 299-303. PMID: 2677740, DOI: 10.1038/341299a0, ISSN: 0028-0836.

Whelan J, Poon D, Weil PA, Stein R. Pancreatic beta-cell-type-specific expression of the rat insulin II gene is controlled by positive and negative cellular transcriptional elements. Mol. Cell. Biol. 1989 Aug; 9(8): 3253-9. PMID: 2552288, PMCID: PMC362369, ISSN: 0270-7306.

Horikoshi M, Wang CK, Fujii H, Cromlish JA, Weil PA, Roeder RG. Purification of a yeast TATA box-binding protein that exhibits human transcription factor IID activity. Proc. Natl. Acad. Sci. U.S.A. 1989 Jul; 86(13): 4843-7. PMID: 2662184, PMCID: PMC297511, ISSN: 0027-8424.

Karnitz L, Poon D, Weil PA, Chalkley R. Purification and properties of the Rous sarcoma virus internal enhancer binding factor. Mol. Cell. Biol. 1989 May; 9(5): 1929-39. PMID: 2546054, PMCID: PMC362984, ISSN: 0270-7306.

Wang CK, Weil PA. Purification and characterization of Saccharomyces cerevisiae transcription factor IIIA. J. Biol. Chem. 1989 Jan 1/15/1989; 264(2): 1092-9. PMID: 2642897, ISSN: 0021-9258.

Klekamp MS, Weil PA. Properties of yeast class III gene transcription factor TFIIIB. Implications regarding mechanism of action. J. Biol. Chem. 1987 Jun 6/5/1987; 262(16): 7878-83. PMID: 3584145, ISSN: 0021-9258.

Felts SJ, Weil PA, Chalkley R. Novobiocin inhibits interactions required for yeast TFIIIB sequestration during stable transcription complex formation in vitro. Nucleic Acids Res. 1987 Feb 2/25/1987; 15(4): 1493-506. PMID: 3547336, PMCID: PMC340563, ISSN: 0305-1048.

Klekamp MS, Weil PA. Yeast class III gene transcription factors and homologous RNA polymerase III form ternary transcription complexes stable to disruption by N-lauroyl-sarcosine (sarcosyl). Arch. Biochem. Biophys. 1986 May 5/1/1986; 246(2): 783-800. PMID: 2423033, ISSN: 0003-9861.

Klekamp MS, Weil PA. Partial purification and characterization of the Saccharomyces cerevisiae transcription factor TFIIIB. J. Biol. Chem. 1986 Feb 2/25/1986; 261(6): 2819-27. PMID: 3512543, ISSN: 0021-9258.

Klekamp MS, Weil PA. Specific transcription of homologous class III genes in yeast-soluble cell-free extracts. J. Biol. Chem. 1982 Jul 7/25/1982; 257(14): 8432-41. PMID: 7045122, ISSN: 0021-9258.

Matsui T, Segall J, Weil PA, Roeder RG. Multiple factors required for accurate initiation of transcription by purified RNA polymerase II. J. Biol. Chem. 1980 Dec 12/25/1980; 255(24): 11992-6. PMID: 7440580, ISSN: 0021-9258.

Weil PA, Luse DS, Segall J, Roeder RG. Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA. Cell. 1979 Oct; 18(2): 469-84. PMID: 498279, PII: 0092-8674(79)90065-5, ISSN: 0092-8674.

Weil PA, Segall J, Harris B, Ng SY, Roeder RG. Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates. J. Biol. Chem. 1979 Jul 7/10/1979; 254(13): 6163-73. PMID: 447704, ISSN: 0021-9258.

Weil PA, Sidikaro J, Stancel GM, Blatti SP. Hormonal control of transcription in the rat uterus. Stimulation of deoxyribonucleic acid-dependent RNA polymerase III by estradiol. J. Biol. Chem. 1977 Feb 2/10/1977; 252(3): 1092-8. PMID: 838697, ISSN: 0021-9258.

Weil PA, Blatti SP. HeLa cell deoxyribonucleic acid dependent RNA polymerases: function and properties of the class III enzymes. Biochemistry. 1976 Apr 4/6/1976; 15(7): 1500-9. PMID: 1259952, ISSN: 0006-2960.

Weil PA, Blatti SP. Partial purification and properties of calf thymus deoxyribonucleic acid dependent RNA polymerase III. Biochemistry. 1975 Apr 4/22/1975; 14(8): 1636-42. PMID: 1125192, ISSN: 0006-2960.

Weil PA, Hampel A. Preparative agarose gel electrophoresis of ribonucleic acid. Biochemistry. 1973 Oct 10/23/1973; 12(22): 4361-7. PMID: 4750249, ISSN: 0006-2960.

Available Postdoctoral Position Details
Posted: 9/17/2010

Two postdoctoral positions available to examine the role of TBP, the TATA box Binding Protein and its associated protein factors in RNA Polymerase II-mediated transcription initiation using the Bakers yeast Saccharomyces cerevisiae system. We currently utilize a combination of biochemical, genetic, biophysical and proteomics methods to study the regulation and interactions of these proteins with each other, DNA and other transcription factors; details can be obtained at: www.mc.vanderbilt.edu/vumcdept/mpb/weil/weil_lab.html.Interested parties should send a CV and names of references to: Dr. Tony Weil, Department of Molecular Physiology & Biophysics, Vanderbilt University, School of Medicine, Nashville, TN 37232-0615. Phone: 615-322-7007, FAX: 615-322-7236; Email: tony.weil@mcmail.vanderbilt.edu.