Joey V. Barnett, Ph.D.

Professor

joey.barnett@vanderbilt.edu

Visit Lab Site


Faculty Appointments
Professor of Pharmacology Professor of MedicineProfessor of Pathology, Microbiology and ImmunologyProfessor of Pediatrics
Education
Ph.D., Pharmacology, Vanderbilt University, Nashville, TennesseeB.S., Biology, University of Southern Indiana, Evansville, Indiana
Office Address
460 PRB
2220 Pierce Avenue
TN 6600
Research Description
The Barnett lab has worked for a number of years on Transforming Growth Factor Beta (TGFß) biology and in the context of cardiac development. The Type III TGFß Receptor (TGFßR3), also known as betaglycan, is a ubiquitously expressed TGFß & BMP receptor that plays an essential role in regulating diverse cellular responses in cardiac development, cancer and angiogenesis. TGFßR3 functions as a rheostat balancing opposing signals downstream of TGFß & BMP receptors, and is down regulated in the lungs of patients with pulmonary arterial hypertension (PAH). PAH is a fatal disease for which no disease modifying therapies have been developed. Clues to the pathogenesis and disease modifying therapeutics for PAH may be gained from the analysis of patients with heritable (HPAH). About 80% of patients with HPAH have mutations in bone morphogenetic protein receptor type 2 (BMPR2), and patients with non-hereditary forms of PAH have decreased BMPR2 expression and signaling. BMPR2 is a member of the BMP and Transforming Growth Factor ß (TGFß) superfamily. It is expressed at high levels in pulmonary endothelial cells (PECs) and conditional deletion of Bmpr2 from PECs results in pulmonary hypertension (PH) in mice. Mutations in other BMP signaling genes that are restricted to the endothelium are also associated with HPAH. This suggests that endothelial cells may be one of the primary sites of cellular dysfunction in patients with HPAH and other forms of PAH. As seen in mice with Bmpr2 mutations, we have shown that Tgfbr3+/- mice develop PH on Western diets. In addition, Tgfbr3+/- cells have the same changes in metabolic reprogramming seen in Bmpr2+/- PECs. We also provide evidence that TGFßR3 over-expression rescues metabolic reprogramming in Bmpr2+/- PECs and that this is associated with increased expression of the master regulator of mitochondrial biogenesis, PGC1a. These findings pose fundamental questions about the role of BMPR2 & TGFßR3 cross talk in PAH pathogenesis, answers to which may lead to better understanding of the mechanisms underlying PAH and identification of novel therapeutic targets.

Research Keywords
Cardiac function, Developmental biology, Heart, Kinase, Knockout, Molecular medicine, Pharmacology, Phosphorylation, Physiology, Receptor, Signal transduction, Vascular Biology, Pulmonary hypertension
Publications
Mignemi NA, Yuasa M, Baker CE, Moore SN, Ihejirika RC, Oelsner WK, Wallace CS, Yoshii T, Okawa A, Revenko AS, MacLeod AF, Bhattacharjee G, Barnett JV, Schwartz HS, Degen JL, Flick MJ, Cates JM, Schoenecker JG. Plasmin Prevents Dystrophic Calcification after Muscle Injury. J. Bone Miner. Res [print-electronic]. 2016 Aug 8/17/2016; PMID: 27530373, DOI: 10.1002/jbmr.2973, ISSN: 1523-4681.

Clark CR, Robinson JY, Sanchez NS, Townsend TA, Arrieta JA, Merryman WD, Trykall DZ, Olivey HE, Hong CC, Barnett JV. Common pathways regulate type III TGFß receptor-dependent cell invasion in epicardial and endocardial cells. Cell. Signal [print-electronic]. 2016 Mar 3/9/2016; PMID: 26970186, PII: S0898-6568(16)30057-2, DOI: 10.1016/j.cellsig.2016.03.004, ISSN: 1873-3913.

McFalls A, Barnett JV, Karpa K. 2015 Meeting of the National Directors of Graduate Studies in Pharmacology and Physiology. Advances in Physiology Education. 2016 Mar 3/1/2016; 40: 134-41.

DeLaughter DM, Clark CR, Christodoulou DC, Seidman CE, Baldwin HS, Seidman JG, Barnett JV. Transcriptional Profiling of Cultured, Embryonic Epicardial Cells Identifies Novel Genes and Signaling Pathways Regulated by TGFßR3 In Vitro. PLoS ONE. 2016; 11(8): e0159710. PMID: 27505173, PMCID: PMC4978490, PII: PONE-D-15-48496, DOI: 10.1371/journal.pone.0159710, ISSN: 1932-6203.

Yuasa M, Mignemi NA, Nyman JS, Duvall CL, Schwartz HS, Okawa A, Yoshii T, Bhattacharjee G, Zhao C, Bible JE, Obremskey WT, Flick MJ, Degen JL, Barnett JV, Cates JM, Schoenecker JG. Fibrinolysis is essential for fracture repair and prevention of heterotopic ossification. J. Clin. Invest [print-electronic]. 2015 Aug 8/3/2015; 125(8): 3117-31. PMID: 26214526, PMCID: PMC4563750, PII: 80313, DOI: 10.1172/JCI80313, ISSN: 1558-8238.

Allison P, Espiritu D, Barnett JV, Camenisch TD. Type III TGFß receptor and Src direct hyaluronan-mediated invasive cell motility. Cell. Signal [print-electronic]. 2015 Mar; 27(3): 453-9. PMID: 25499979, PII: S0898-6568(14)00400-8, DOI: 10.1016/j.cellsig.2014.11.037, ISSN: 1873-3913.

Hill CR, Jacobs BH, Brown CB, Barnett JV, Goudy SL. Type III transforming growth factor beta receptor regulates vascular and osteoblast development during palatogenesis. Dev. Dyn [print-electronic]. 2015 Feb; 244(2): 122-33. PMID: 25382630, PMCID: PMC4310801, DOI: 10.1002/dvdy.24225, ISSN: 1097-0177.

Sidorova TN, Yermalitskaya LV, Mace LC, Wells KS, Boutaud O, Prinsen JK, Davies SS, Roberts LJ, Dikalov SI, Glabe CG, Amarnath V, Barnett JV, Murray KT. Reactive ¿-ketoaldehydes promote protein misfolding and preamyloid oligomer formation in rapidly-activated atrial cells. J. Mol. Cell. Cardiol [print-electronic]. 2015 Feb; 79: 295-302. PMID: 25463275, PMCID: PMC4302000, PII: S0022-2828(14)00373-3, DOI: 10.1016/j.yjmcc.2014.11.013, ISSN: 1095-8584.

Jacobson R, Mignemi N, Rose K, O'Rear L, Sarilla S, Hamm HE, Barnett JV, Verhamme IM, Schoenecker J. The hyperglycemic byproduct methylglyoxal impairs anticoagulant activity through covalent adduction of antithrombin III. Thromb. Res [print-electronic]. 2014 Dec; 134(6): 1350-7. PMID: 25307422, PMCID: PMC4337957, PII: S0049-3848(14)00523-4, DOI: 10.1016/j.thromres.2014.09.038, ISSN: 1879-2472.

Sidorova TN, Mace LC, Wells KS, Yermalitskaya LV, Su PF, Shyr Y, Atkinson JB, Fogo AB, Prinsen JK, Byrne JG, Petracek MR, Greelish JP, Hoff SJ, Ball SK, Glabe CG, Brown NJ, Barnett JV, Murray KT. Hypertension is associated with preamyloid oligomers in human atrium: a missing link in atrial pathophysiology?. J Am Heart Assoc. 2014 Dec; 3(6): e001384. PMID: 25468655, PMCID: PMC4338732, PII: jah3772, DOI: 10.1161/JAHA.114.001384, ISSN: 2047-9980.

Huang T, Barnett JV, Camenisch TD. Cardiac epithelial-mesenchymal transition is blocked by monomethylarsonous acid (III). Toxicol. Sci [print-electronic]. 2014 Nov; 142(1): 225-38. PMID: 25145660, PMCID: PMC4334812, PII: kfu170, DOI: 10.1093/toxsci/kfu170, ISSN: 1096-0929.

Yuasa M, Mignemi NA, Barnett JV, Cates JM, Nyman JS, Okawa A, Yoshii T, Schwartz HS, Stutz CM, Schoenecker JG. The temporal and spatial development of vascularity in a healing displaced fracture. Bone [print-electronic]. 2014 Oct; 67: 208-21. PMID: 25016962, PII: S8756-3282(14)00253-1, DOI: 10.1016/j.bone.2014.07.002, ISSN: 1873-2763.

Sidorova TN, Mace LC, Wells KS, Yermalitskaya LV, Su PF, Shyr Y, Byrne JG, Petracek MR, Greelish JP, Hoff SJ, Ball SK, Glabe CG, Brown NJ, Barnett JV, Murray KT. Quantitative Imaging of Preamyloid Oligomers, a Novel Structural Abnormality, in Human Atrial Samples. J. Histochem. Cytochem [print-electronic]. 2014 Apr 4/30/2014; 62(7): 479-87. PMID: 24789805, PMCID: PMC4072180, PII: 0022155414535782, DOI: 10.1369/0022155414535782, ISSN: 1551-5044.

Sewell-Loftin MK, DeLaughter DM, Peacock JR, Brown CB, Baldwin HS, Barnett JV, Merryman WD. Myocardial contraction and hyaluronic acid mechanotransduction in epithelial-to-mesenchymal transformation of endocardial cells. Biomaterials [print-electronic]. 2014 Mar; 35(9): 2809-15. PMID: 24433835, PMCID: PMC3950274, PII: S0142-9612(13)01535-4, DOI: 10.1016/j.biomaterials.2013.12.051, ISSN: 1878-5905.

Kain KH, Miller JW, Jones-Paris CR, Thomason RT, Lewis JD, Bader DM, Barnett JV, Zijlstra A. The chick embryo as an expanding experimental model for cancer and cardiovascular research. Dev. Dyn [print-electronic]. 2014 Feb; 243(2): 216-28. PMID: 24357262, PMCID: PMC4164046, DOI: 10.1002/dvdy.24093, ISSN: 1097-0177.

Allison P, Huang T, Broka D, Parker P, Barnett JV, Camenisch TD. Disruption of canonical TGFß-signaling in murine coronary progenitor cells by low level arsenic. Toxicol. Appl. Pharmacol [print-electronic]. 2013 Oct 10/1/2013; 272(1): 147-53. PMID: 23732083, PMCID: PMC3972124, PII: S0041-008X(13)00219-6, DOI: 10.1016/j.taap.2013.04.035, ISSN: 1096-0333.

DeLaughter DM, Christodoulou DC, Robinson JY, Seidman CE, Baldwin HS, Seidman JG, Barnett JV. Spatial transcriptional profile of the chick and mouse endocardial cushions identify novel regulators of endocardial EMT in vitro. J. Mol. Cell. Cardiol [print-electronic]. 2013 Jun; 59: 196-204. PMID: 23557753, PMCID: PMC3659811, PII: S0022-2828(13)00097-7, DOI: 10.1016/j.yjmcc.2013.03.016, ISSN: 1095-8584.

Zhang HF, Lin XH, Yang H, Zhou LC, Guo YL, Barnett JV, Guo ZM. Regulation of the activity and expression of aryl hydrocarbon receptor by ethanol in mouse hepatic stellate cells. Alcohol. Clin. Exp. Res [print-electronic]. 2012 Nov; 36(11): 1873-81. PMID: 22486318, PMCID: PMC3462894, DOI: 10.1111/j.1530-0277.2012.01787.x, ISSN: 1530-0277.

Hill CR, Sanchez NS, Love JD, Arrieta JA, Hong CC, Brown CB, Austin AF, Barnett JV. BMP2 signals loss of epithelial character in epicardial cells but requires the Type III TGFß receptor to promote invasion. Cell. Signal [print-electronic]. 2012 May; 24(5): 1012-22. PMID: 22237159, PMCID: PMC3288519, PII: S0898-6568(11)00414-1, DOI: 10.1016/j.cellsig.2011.12.022, ISSN: 1873-3913.

Sánchez NS, Barnett JV. TGFß and BMP-2 regulate epicardial cell invasion via TGFßR3 activation of the Par6/Smurf1/RhoA pathway. Cell. Signal [print-electronic]. 2012 Feb; 24(2): 539-48. PMID: 22033038, PMCID: PMC3237859, PII: S0898-6568(11)00334-2, DOI: 10.1016/j.cellsig.2011.10.006, ISSN: 1873-3913.

Townsend TA, Robinson JY, How T, DeLaughter DM, Blobe GC, Barnett JV. Endocardial cell epithelial-mesenchymal transformation requires Type III TGFß receptor interaction with GIPC. Cell. Signal [print-electronic]. 2012 Jan; 24(1): 247-56. PMID: 21945156, PMCID: PMC3208316, PII: S0898-6568(11)00285-3, DOI: 10.1016/j.cellsig.2011.09.006, ISSN: 1873-3913.

Doetschman T, Barnett JV, Runyan RB, Camenisch TD, Heimark RL, Granzier HL, Conway SJ, Azhar M. Transforming growth factor beta signaling in adult cardiovascular diseases and repair. Cell Tissue Res [print-electronic]. 2012 Jan; 347(1): 203-23. PMID: 21953136, PMCID: PMC3328790, DOI: 10.1007/s00441-011-1241-3, ISSN: 1432-0878.

Lencinas A, Tavares AL, Barnett JV, Runyan RB. Collagen gel analysis of epithelial-mesenchymal transition in the embryo heart: an in vitro model system for the analysis of tissue interaction, signal transduction, and environmental effects. Birth Defects Res. C Embryo Today. 2011 Dec; 93(4): 298-311. PMID: 22271679, DOI: 10.1002/bdrc.20222, ISSN: 1542-9768.

Sánchez NS, Hill CR, Love JD, Soslow JH, Craig E, Austin AF, Brown CB, Czirok A, Camenisch TD, Barnett JV. The cytoplasmic domain of TGFßR3 through its interaction with the scaffolding protein, GIPC, directs epicardial cell behavior. Dev. Biol [print-electronic]. 2011 Oct 10/15/2011; 358(2): 331-43. PMID: 21871877, PMCID: PMC3183347, PII: S0012-1606(11)01195-X, DOI: 10.1016/j.ydbio.2011.08.008, ISSN: 1095-564X.

DeLaughter DM, Saint-Jean L, Baldwin HS, Barnett JV. What chick and mouse models have taught us about the role of the endocardium in congenital heart disease. Birth Defects Res. Part A Clin. Mol. Teratol [print-electronic]. 2011 Jun; 91(6): 511-25. PMID: 21538818, DOI: 10.1002/bdra.20809, ISSN: 1542-0760.

Angel PM, Nusinow D, Brown CB, Violette K, Barnett JV, Zhang B, Baldwin HS, Caprioli RM. Networked-based characterization of extracellular matrix proteins from adult mouse pulmonary and aortic valves. J. Proteome Res [print-electronic]. 2011 Feb 2/4/2011; 10(2): 812-23. PMID: 21133377, PMCID: PMC3139330, DOI: 10.1021/pr1009806, ISSN: 1535-3907.

Townsend TA, Robinson JY, Deig CR, Hill CR, Misfeldt A, Blobe GC, Barnett JV. BMP-2 and TGFß2 shared pathways regulate endocardial cell transformation. Cells Tissues Organs (Print) [print-electronic]. 2011; 194(1): 1-12. PMID: 21212630, PMCID: PMC3128155, PII: 000322035, DOI: 10.1159/000322035, ISSN: 1422-6421.

Barnett, JV. . Ph.D. education for biomedical scientists in the United States. Proceedings, of the 6th ORPHEUS Conference. Turkish Journal of Biochemistry. 2011; ((36)): 9-12.

Craig EA, Austin AF, Vaillancourt RR, Barnett JV, Camenisch TD. TGFß2-mediated production of hyaluronan is important for the induction of epicardial cell differentiation and invasion. Exp. Cell Res [print-electronic]. 2010 Dec 12/10/2010; 316(20): 3397-405. PMID: 20633555, PMCID: PMC3397912, PII: S0014-4827(10)00368-X, DOI: 10.1016/j.yexcr.2010.07.006, ISSN: 1090-2422.

Frieden LA, Townsend TA, Vaught DB, Delaughter DM, Hwang Y, Barnett JV, Chen J. Regulation of heart valve morphogenesis by Eph receptor ligand, ephrin-A1. Dev. Dyn [print-electronic]. 2010 Dec; 239(12): 3226-34. PMID: 20960543, PMCID: PMC3023820, DOI: 10.1002/dvdy.22458, ISSN: 1097-0177.

Craig EA, Parker P, Austin AF, Barnett JV, Camenisch TD. Involvement of the MEKK1 signaling pathway in the regulation of epicardial cell behavior by hyaluronan. Cell. Signal [print-electronic]. 2010 Jun; 22(6): 968-76. PMID: 20159036, PMCID: PMC2846756, PII: S0898-6568(10)00057-4, DOI: 10.1016/j.cellsig.2010.02.004, ISSN: 1873-3913.

Karunamuni G, Yang K, Doughman YQ, Wikenheiser J, Bader D, Barnett J, Austin A, Parsons-Wingerter P, Watanabe M. Expression of lymphatic markers during avian and mouse cardiogenesis. Anat Rec (Hoboken). 2010 Feb; 293(2): 259-70. PMID: 19938109, PMCID: PMC3607319, DOI: 10.1002/ar.21043, ISSN: 1932-8494.

Criswell TL, Dumont N, Barnett JV, Arteaga CL. Knockdown of the transforming growth factor-beta type III receptor impairs motility and invasion of metastatic cancer cells. Cancer Res. 2008 Sep 9/15/2008; 68(18): 7304-12. PMID: 18794117, PII: 68/18/7304, DOI: 10.1158/0008-5472.CAN-07-6777, ISSN: 1538-7445.

Townsend TA, Wrana JL, Davis GE, Barnett JV. Transforming growth factor-beta-stimulated endocardial cell transformation is dependent on Par6c regulation of RhoA. J. Biol. Chem [print-electronic]. 2008 May 5/16/2008; 283(20): 13834-41. PMID: 18343818, PMCID: PMC2376225, PII: M710607200, DOI: 10.1074/jbc.M710607200, ISSN: 0021-9258.

Kirkbride KC, Townsend TA, Bruinsma MW, Barnett JV, Blobe GC. Bone morphogenetic proteins signal through the transforming growth factor-beta type III receptor. J. Biol. Chem [print-electronic]. 2008 Mar 3/21/2008; 283(12): 7628-37. PMID: 18184661, PII: M704883200, DOI: 10.1074/jbc.M704883200, ISSN: 0021-9258.

Austin AF, Compton LA, Love JD, Brown CB, Barnett JV. Primary and immortalized mouse epicardial cells undergo differentiation in response to TGFbeta. Dev. Dyn. 2008 Feb; 237(2): 366-76. PMID: 18213583, DOI: 10.1002/dvdy.21421, ISSN: 1058-8388.

James FD, Hietala KA, Eldar D, Guess TE, Cone C, Mundell NA, Barnett JV, Raju R. Efficient replication, and evolution of Sindbis virus genomes with non-canonical 3'A/U-rich elements (NC3ARE) in neonatal mice. Virus Genes [print-electronic]. 2007 Dec; 35(3): 651-62. PMID: 17616797, DOI: 10.1007/s11262-007-0130-z, ISSN: 0920-8569.

Compton LA, Potash DA, Brown CB, Barnett JV. Coronary vessel development is dependent on the type III transforming growth factor beta receptor. Circ. Res [print-electronic]. 2007 Oct 10/12/2007; 101(8): 784-91. PMID: 17704211, PII: CIRCRESAHA.107.152082, DOI: 10.1161/CIRCRESAHA.107.152082, ISSN: 1524-4571.

Park HJ, Ward SM, Desgrosellier JS, Georgescu SP, Papageorge AG, Zhuang X, Barnett JV, Galper JB. Transforming growth factor beta regulates the expression of the M2 muscarinic receptor in atrial myocytes via an effect on RhoA and p190RhoGAP. J. Biol. Chem [print-electronic]. 2006 Jul 7/21/2006; 281(29): 19995-20002. PMID: 16707504, PII: M513095200, DOI: 10.1074/jbc.M513095200, ISSN: 0021-9258.

Barnett J. Directors of pharmacology graduate programs: Pharm Phorum. Mol. Interv. 2006 Feb; 6(1): 4-7. PMID: 16507743, PII: 6/1/4, DOI: 10.1124/mi.6.1.1, ISSN: 1534-0384.

Compton LA, Potash DA, Mundell NA, Barnett JV. Transforming growth factor-beta induces loss of epithelial character and smooth muscle cell differentiation in epicardial cells. Dev. Dyn. 2006 Jan; 235(1): 82-93. PMID: 16258965, DOI: 10.1002/dvdy.20629, ISSN: 1058-8388.

Olivey HE, Mundell NA, Austin AF, Barnett JV. Transforming growth factor-beta stimulates epithelial-mesenchymal transformation in the proepicardium. Dev. Dyn. 2006 Jan; 235(1): 50-9. PMID: 16245329, PMCID: PMC3160345, DOI: 10.1002/dvdy.20593, ISSN: 1058-8388.

Desgrosellier JS, Mundell NA, McDonnell MA, Moses HL, Barnett JV. Activin receptor-like kinase 2 and Smad6 regulate epithelial-mesenchymal transformation during cardiac valve formation. Dev. Biol. 2005 Apr 4/1/2005; 280(1): 201-10. PMID: 15766759, PII: S0012-1606(05)00048-5, DOI: 10.1016/j.ydbio.2004.12.037, ISSN: 0012-1606.

Olivey HE, Compton LA, Barnett JV. Coronary vessel development: the epicardium delivers. Trends Cardiovasc. Med. 2004 Aug; 14(6): 247-51. PMID: 15451517, PII: S1050-1738(04)00110-0, DOI: 10.1016/j.tcm.2004.07.001, ISSN: 1050-1738.

Olivey HE, Barnett JV, Ridley BD. Expression of the type III TGFbeta receptor during chick organogenesis. Anat Rec A Discov Mol Cell Evol Biol. 2003 May; 272(1): 383-7. PMID: 12704694, DOI: 10.1002/ar.a.10049, ISSN: 1552-4884.

Marlow MS, Brown CB, Barnett JV, Krezel AM. Solution structure of the chick TGFbeta type II receptor ligand-binding domain. J. Mol. Biol. 2003 Feb 2/28/2003; 326(4): 989-97. PMID: 12589747, PII: S0022283603000238, ISSN: 0022-2836.

Barnett JV, Desgrosellier JS. Early events in valvulogenesis: a signaling perspective. Birth Defects Res. C Embryo Today. 2003 Feb; 69(1): 58-72. PMID: 12768658, DOI: 10.1002/bdrc.10006, ISSN: 1542-975X.

Ward SM, Desgrosellier JS, Zhuang X, Barnett JV, Galper JB. Transforming growth factor beta (TGFbeta ) signaling via differential activation of activin receptor-like kinases 2 and 5 during cardiac development. Role in regulating parasympathetic responsiveness. J. Biol. Chem [print-electronic]. 2002 Dec 12/20/2002; 277(51): 50183-9. PMID: 12393881, PII: M209668200, DOI: 10.1074/jbc.M209668200, ISSN: 0021-9258.

Ward SM, Gadbut AP, Tang D, Papageorge AG, Wu L, Li G, Barnett JV, Galper JB. TGFbeta regulates the expression of G alpha(i2) via an effect on the localization of ras. J. Mol. Cell. Cardiol. 2002 Sep; 34(9): 1217-26. PMID: 12392895, PII: S0022282802920730, ISSN: 0022-2828.

Jeyakumar LH, Gleaves LA, Ridley BD, Chang P, Atkinson J, Barnett JV, Fleischer S. The skeletal muscle ryanodine receptor isoform 1 is found at the intercalated discs in human and mouse hearts. J. Muscle Res. Cell. Motil. 2002; 23(4): 285-92. PMID: 12630702, ISSN: 0142-4319.

Bushdid PB, Chen CL, Brantley DM, Yull F, Raghow R, Kerr LD, Barnett JV. NF-kappaB mediates FGF signal regulation of msx-1 expression. Dev. Biol. 2001 Sep 9/1/2001; 237(1): 107-15. PMID: 11518509, PII: S0012-1606(01)90356-2, DOI: 10.1006/dbio.2001.0356, ISSN: 0012-1606.

Jeyakumar LH, Ballester L, Cheng DS, McIntyre JO, Chang P, Olivey HE, Rollins-Smith L, Barnett JV, Murray K, Xin HB, Fleischer S. FKBP binding characteristics of cardiac microsomes from diverse vertebrates. Biochem. Biophys. Res. Commun. 2001 Mar 3/9/2001; 281(4): 979-86. PMID: 11237759, PII: S0006-291X(01)94444-4, DOI: 10.1006/bbrc.2001.4444, ISSN: 0006-291X.

Shin HG, Barnett JV, Chang P, Reddy S, Drinkwater DC, Pierson RN, Wiley RG, Murray KT. Molecular heterogeneity of protein kinase C expression in human ventricle. Cardiovasc. Res. 2000 Nov; 48(2): 285-99. PMID: 11054475, PII: S0008-6363(00)00185-1, ISSN: 0008-6363.

Marlow MS, Chim N, Brown CB, Barnett JV, Krezel AM. 1H, 13C, and 15N backbone assignments of the ligand binding domain of TGFbeta type II receptor. J. Biomol. NMR. 2000 Aug; 17(4): 349-50. PMID: 11014599, ISSN: 0925-2738.

Bulus NM, Sheng HM, Sizemore N, Oldham SM, Barnett JV, Coffey RJ, Beauchamp DR, Barnard JA. Ras-mediated suppression of TGFbetaRII expression in intestinal epithelial cells involves Raf-independent signaling. Neoplasia. 2000 Jul; 2(4): 357-64. PMID: 11005570, PMCID: PMC1550294, ISSN: 1522-8002.

Lai YT, Beason KB, Brames GP, Desgrosellier JS, Cleggett MC, Shaw MV, Brown CB, Barnett JV. Activin receptor-like kinase 2 can mediate atrioventricular cushion transformation. Dev. Biol. 2000 Jun 6/1/2000; 222(1): 1-11. PMID: 10885742, PII: S0012-1606(00)99698-2, DOI: 10.1006/dbio.2000.9698, ISSN: 0012-1606.

Brown CB, Drake CJ, Barnett JV. Antibodies directed against the chicken type II TGFbeta receptor identify endothelial cells in the developing chicken and quail. Dev. Dyn. 1999 May; 215(1): 79-85. PMID: 10340759, PII: 10.1002/(SICI)1097-0177(199905)215:1<79::AID-DVDY9>3.0.CO;2-H, DOI: 10.1002/(SICI)1097-0177(199905)215:1<79::AID-DVDY9>3.0.CO;2-H, ISSN: 1058-8388.

Brown CB, Boyer AS, Runyan RB, Barnett JV. Requirement of type III TGF-beta receptor for endocardial cell transformation in the heart. Science. 1999 Mar 3/26/1999; 283(5410): 2080-2. PMID: 10092230, ISSN: 0036-8075.

Brown CB, Boyer AS, Runyan RB, Barnett JV. Antibodies to the Type II TGFbeta receptor block cell activation and migration during atrioventricular cushion transformation in the heart. Dev. Biol. 1996 Mar 3/15/1996; 174(2): 248-57. PMID: 8631497, PII: S0012-1606(96)90070-6, DOI: 10.1006/dbio.1996.0070, ISSN: 0012-1606.

Loh E, Barnett JV, Feldman AM, Couper GS, Vatner DE, Colucci WS, Galper JB. Decreased adenylate cyclase activity and expression of Gs alpha in human myocardium after orthotopic cardiac transplantation. Circ. Res. 1995 May; 76(5): 852-60. PMID: 7729002, ISSN: 0009-7330.

Barnett JV, Moustakas A, Lin W, Wang XF, Lin HY, Galper JB, Maas RL. Cloning and developmental expression of the chick type II and type III TGF beta receptors. Dev. Dyn. 1994 Jan; 199(1): 12-27. PMID: 8167376, DOI: 10.1002/aja.1001990103, ISSN: 1058-8388.

Tan W, Barnett JV, Pietrobon D, Hehn G, Greiser C, Marsh JD, Galper JB. Effect of low-density lipoproteins, mevinolin, and G proteins on Ca2+ response in cultured chick atrial cells. Am. J. Physiol. 1993 Jul; 265(1 Pt 2): H191-7. PMID: 7688189, ISSN: 0002-9513.

Barnett JV, Taniuchi M, Yang MB, Galper JB. Co-culture of embryonic chick heart cells and ciliary ganglia induces parasympathetic responsiveness in embryonic chick heart cells. Biochem. J. 1993 Jun 6/1/1993; 292 ( Pt 2): 395-9. PMID: 8503875, PMCID: PMC1134222, ISSN: 0264-6021.

Ogawa S, Barnett JV, Sen L, Galper JB, Smith TW, Marsh JD. Direct contact between sympathetic neurons and rat cardiac myocytes in vitro increases expression of functional calcium channels. J. Clin. Invest. 1992 Apr; 89(4): 1085-93. PMID: 1313444, PMCID: PMC442964, DOI: 10.1172/JCI115688, ISSN: 0021-9738.

Tan W, Barnett JV, Hehn GM, Quay SC, Galper JB. Effect of manganese (II) bis(glycinate)dichloride on Ca2+ channel function in cultured chick atrial cells. Toxicology. 1991; 68(1): 63-73. PMID: 1714640, ISSN: 0300-483X.

Barnett JV, Shamah SM, Lassegue B, Griendling KK, Galper JB. Development of muscarinic-cholinergic stimulation of inositol phosphate production in cultured embryonic chick atrial cells. Evidence for a switch in guanine-nucleotide-binding protein coupling. Biochem. J. 1990 Oct 10/15/1990; 271(2): 443-8. PMID: 2122888, PMCID: PMC1149574, ISSN: 0264-6021.

Barnett JV, Shamah SM, Lassegue B, Griendling KK, Galper JB. Muscarinic cholinergic stimulation of inositol phosphate production in cultured embryonic chick atrial cells. Evidence for a role of two guanine-nucleotide-binding proteins. Biochem. J. 1990 Oct 10/15/1990; 271(2): 437-42. PMID: 2122887, PMCID: PMC1149573, ISSN: 0264-6021.

Barnett JV, Shamah SM, Galper JB. The development of physiologic responsiveness to muscarinic stimulation in embryonic chick heart. Relationship to increased levels of pertussis toxin substrates. Ann. N. Y. Acad. Sci. 1990; 588: 145-54. PMID: 2113368, ISSN: 0077-8923.

Barnett JV, Haigh LS, Marsh JD, Galper JB. Effects of low density lipoproteins and mevinolin on sympathetic responsiveness in cultured chick atrial cells. Regulation of beta-adrenergic receptors and alpha s. J. Biol. Chem. 1989 Jun 6/25/1989; 264(18): 10779-86. PMID: 2471706, ISSN: 0021-9258.

Chen CJ, Barnett JV, Congo DA, Brown EM. Divalent cations suppress 3',5'-adenosine monophosphate accumulation by stimulating a pertussis toxin-sensitive guanine nucleotide-binding protein in cultured bovine parathyroid cells. Endocrinology. 1989 Jan; 124(1): 233-9. PMID: 2462488, DOI: 10.1210/endo-124-1-233, ISSN: 0013-7227.

Barnett JV, Segal DS, Kuczenski R. Repeated amphetamine pretreatment alters the responsiveness of striatal dopamine-stimulated adenylate cyclase to amphetamine-induced desensitization. J. Pharmacol. Exp. Ther. 1987 Jul; 242(1): 40-7. PMID: 2956412, ISSN: 0022-3565.

Barnett JV, Kuczenski R. Desensitization of rat striatal dopamine-stimulated adenylate cyclase after acute amphetamine administration. J. Pharmacol. Exp. Ther. 1986 Jun; 237(3): 820-5. PMID: 2940359, ISSN: 0022-3565.