Eric J. Hustedt, Ph.D.

Research Associate Professor

eric.hustedt@vanderbilt.edu

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Faculty Appointments
Research Associate Professor of Molecular Physiology and Biophysics
Education
Ph.D., Physical Chemistry, University of Washington, Seattle, WashingtonB.A., Chemistry, Reed College, Portland, Oregon
Office Address
735B Light Hall
TN 0615
Publications
Dixit M, Kim S, Matthews GF, Erreger K, Galli A, Cobb CE, Hustedt EJ, Beth AH. Structural arrangement of the intracellular Ca2+ binding domains of the cardiac Na+/Ca2+ exchanger (NCX1.1): effects of Ca2+ binding. J. Biol. Chem [print-electronic]. 2013 Feb 2/8/2013; 288(6): 4194-207. PMID: 23233681, PMCID: PMC3567668, PII: M112.423293, DOI: 10.1074/jbc.M112.423293, ISSN: 1083-351X.

Barrett PJ, Song Y, Van Horn WD, Hustedt EJ, Schafer JM, Hadziselimovic A, Beel AJ, Sanders CR. The amyloid precursor protein has a flexible transmembrane domain and binds cholesterol. Science. 2012 Jun 6/1/2012; 336(6085): 1168-71. PMID: 22654059, PMCID: PMC3528355, PII: 336/6085/1168, DOI: 10.1126/science.1219988, ISSN: 1095-9203.

Brandon S, Beth AH, Hustedt EJ. The global analysis of DEER data. J. Magn. Reson [print-electronic]. 2012 May; 218: 93-104. PMID: 22578560, PMCID: PMC3608411, PII: S1090-7807(12)00106-1, DOI: 10.1016/j.jmr.2012.03.006, ISSN: 1096-0856.

Kim S, Brandon S, Zhou Z, Cobb CE, Edwards SJ, Moth CW, Parry CS, Smith JA, Lybrand TP, Hustedt EJ, Beth AH. Determination of structural models of the complex between the cytoplasmic domain of erythrocyte band 3 and ankyrin-R repeats 13-24. J. Biol. Chem [print-electronic]. 2011 Jun 6/10/2011; 286(23): 20746-57. PMID: 21493712, PMCID: PMC3121456, PII: M111.230326, DOI: 10.1074/jbc.M111.230326, ISSN: 1083-351X.

DeSensi SC, Rangel DP, Beth AH, Lybrand TP, Hustedt EJ. Simulation of nitroxide electron paramagnetic resonance spectra from brownian trajectories and molecular dynamics simulations. Biophys. J [print-electronic]. 2008 May 5/15/2008; 94(10): 3798-809. PMID: 18234808, PMCID: PMC2367180, PII: S0006-3495(08)70383-7, DOI: 10.1529/biophysj.107.125419, ISSN: 1542-0086.

Zhou Z, DeSensi SC, Stein RA, Brandon S, Song L, Cobb CE, Hustedt EJ, Beth AH. Structure of the cytoplasmic domain of erythrocyte band 3 hereditary spherocytosis variant P327R: band 3 Tuscaloosa. Biochemistry [print-electronic]. 2007 Sep 9/11/2007; 46(36): 10248-57. PMID: 17696498, DOI: 10.1021/bi700948p, ISSN: 0006-2960.

Hustedt EJ, Stein RA, Sethaphong L, Brandon S, Zhou Z, Desensi SC. Dipolar coupling between nitroxide spin labels: the development and application of a tether-in-a-cone model. Biophys. J [print-electronic]. 2006 Jan 1/1/2006; 90(1): 340-56. PMID: 16214868, PMCID: PMC1367032, PII: S0006-3495(06)72215-9, DOI: 10.1529/biophysj.105.068544, ISSN: 0006-3495.

Zhou Z, DeSensi SC, Stein RA, Brandon S, Dixit M, McArdle EJ, Warren EM, Kroh HK, Song L, Cobb CE, Hustedt EJ, Beth AH. Solution structure of the cytoplasmic domain of erythrocyte membrane band 3 determined by site-directed spin labeling. Biochemistry. 2005 Nov 11/22/2005; 44(46): 15115-28. PMID: 16285715, DOI: 10.1021/bi050931t, ISSN: 0006-2960.

Hustedt EJ, Beth AH. High field/high frequency saturation transfer electron paramagnetic resonance spectroscopy: increased sensitivity to very slow rotational motions. Biophys. J. 2004 Jun; 86(6): 3940-50. PMID: 15189890, PMCID: PMC1304295, PII: S0006-3495(04)74434-3, DOI: 10.1529/biophysj.103.035048, ISSN: 0006-3495.

Stein RA, Hustedt EJ, Staros JV, Beth AH. Rotational dynamics of the epidermal growth factor receptor. Biochemistry. 2002 Feb 2/12/2002; 41(6): 1957-64. PMID: 11827542, PII: bi015733q, ISSN: 0006-2960.

Blackman SM, Hustedt EJ, Cobb CE, Beth AH. Flexibility of the cytoplasmic domain of the anion exchange protein, band 3, in human erythrocytes. Biophys. J. 2001 Dec; 81(6): 3363-76. PMID: 11720999, PMCID: PMC1301793, PII: S0006-3495(01)75969-3, DOI: 10.1016/S0006-3495(01)75969-3, ISSN: 0006-3495.

Hustedt EJ, Beth AH. The sensitivity of saturation transfer electron paramagnetic resonance spectra to restricted amplitude uniaxial rotational diffusion. Biophys. J. 2001 Dec; 81(6): 3156-65. PMID: 11720982, PMCID: PMC1301776, PII: S0006-3495(01)75952-8, DOI: 10.1016/S0006-3495(01)75952-8, ISSN: 0006-3495.

Hustedt EJ, Beth AH. Nitroxide spin-spin interactions: applications to protein structure and dynamics. Annu Rev Biophys Biomol Struct. 1999; 28: 129-53. PMID: 10410798, DOI: 10.1146/annurev.biophys.28.1.129, ISSN: 1056-8700.

E.J. Hustedt, and Beth, A.H. Nitroxide spin-spin interactions: Applications to protein structure and dynamics. Annu. Rev. Biophys. Biomol. Struct. 1999; 28: 129-53.

Hustedt EJ, Smirnov AI, Laub CF, Cobb CE, Beth AH. Molecular distances from dipolar coupled spin-labels: the global analysis of multifrequency continuous wave electron paramagnetic resonance data. Biophys. J. 1997 Apr; 72(4): 1861-77. PMID: 9083690, PMCID: PMC1184380, PII: S0006-3495(97)78832-5, DOI: 10.1016/S0006-3495(97)78832-5, ISSN: 0006-3495.

E.J. Hustedt, Smirnov, A.I., Laub, C.F., and Beth, A.H. Measurement of molecular distances from dipolar coupled EPR spectra: The global analysis of multifrequency continuous wave electron paramagnetic resonance data. Biophys. J. 1997; 74: 1861-77.

P.L. Fischhaber, Reese, A.W., Ngiuem, T., Kirchner, J.J., Hustedt, E.J., Robinson, B.H., and Hopkins, P.B. Synthesis of duplex DNA containing a spin labeled analog of 2'-deoxycytidine. Nucleosides & Nucleotides. 1997; 16: 365-77.

Hustedt EJ, Beth AH. Determination of the orientation of a band 3 affinity spin-label relative to the membrane normal axis of the human erythrocyte. Biochemistry. 1996 May 5/28/1996; 35(21): 6944-54. PMID: 8639646, PII: bi9601518, DOI: 10.1021/bi9601518, ISSN: 0006-2960.

Scothorn DJ, Wojcicki WE, Hustedt EJ, Beth AH, Cobb CE. Synthesis and characterization of a novel spin-labeled affinity probe of human erythrocyte band 3: characteristics of the stilbenedisulfonate binding site. Biochemistry. 1996 May 5/28/1996; 35(21): 6931-43. PMID: 8639645, PII: bi960150f, DOI: 10.1021/bi960150f, ISSN: 0006-2960.

D.J. Scothorn, E.J. Hustedt, W.E. Wojcicki, A.H. Beth, and C.E. Cobb. Synthesis and characterization of a novel spin-labeled affinity probe of human erythrocyte band 3: properties of the stilbenedisulfonate binding site. Biochemistry. 1996; 35: 6944-54.

E.J. Hustedt, and A.H. Beth. The determination of the orientation of a band 3 affinity spin-label relative to the membrane normal axis of the human erythrocyte. Biochemistry. 1996; 35: 6955-62.

A.C., Rybicki, Hustedt, E.J., Schwartz, R.S., and Cobb, C.E. Increased rotational mobility and extractability of band 3 from protein 4.2-deficient erythrocyte membranes: Evidence for a role for protein 4.2 in strengthening the band 3-cytoskeleton linkage. Blood. 1996; 88: 2745-53.

Hustedt EJ, Beth AH. Analysis of saturation transfer electron paramagnetic resonance spectra of a spin-labeled integral membrane protein, band 3, in terms of the uniaxial rotational diffusion model. Biophys. J. 1995 Oct; 69(4): 1409-23. PMID: 8534811, PMCID: PMC1236371, PII: S0006-3495(95)80010-X, DOI: 10.1016/S0006-3495(95)80010-X, ISSN: 0006-3495.

E.J. Hustedt, J.J. Kirchner, A. Spaltenstein, P.B. Hopkins, and B.H. Robinson. Monitoring DNA dynamics using spin labels with different independent mobilities. Biochemistry. 1995; 34: 4369.

E.J. Hustedt and A.H. Beth. The analysis of saturation transfer electron paramagnetic resonance spectra of a spin-labeled integral membrane protein, band 3, in terms of the uniaxial rotational diffsuion model. Biophys. J. 1995; 69: 1409-23.

Hustedt EJ, Cobb CE, Beth AH, Beechem JM. Measurement of rotational dynamics by the simultaneous nonlinear analysis of optical and EPR data. Biophys. J. 1993 Mar; 64(3): 614-21. PMID: 7682452, PMCID: PMC1262373, PII: S0006-3495(93)81420-6, DOI: 10.1016/S0006-3495(93)81420-6, ISSN: 0006-3495.

Cobb CE, Hustedt EJ, Beechem JM, Beth AH. Protein rotational dynamics investigated with a dual EPR/optical molecular probe. Spin-labeled eosin. Biophys. J. 1993 Mar; 64(3): 605-13. PMID: 7682451, PMCID: PMC1262372, PII: S0006-3495(93)81419-X, DOI: 10.1016/S0006-3495(93)81419-X, ISSN: 0006-3495.

E.J. Hustedt, C.E. Cobb, A.H. Beth, and J.M. Beechem. Measurement of rotational dynamics by the simultaneous non-linear analysis of optical and EPR data. Biophys. J. 1993; 64: 614-21.

C.E. Cobb, E.J. Hustedt, J.M. Beechem, and A.H. Beth. Protein rotational dynamics investigated with a dual EPR/optical probe: Spin-labeled eosin. Biopyhs. J. 1993; 64: 605-13.

E.J. Hustedt, A. Schweiger, and R.R. Ernst. Stimulated Soft Electron Spin Echo Envelope Modulation Spectroscopy. J Chem Phys. 1992; 96: 4954.

E.J. Hustedt, A. Spaltenstein, J.J. Kirchner, P.B. Hopkins, and B.H. Robinson. Motions of Short Duplex DNA: An Analysis of DNA Dynamics Using an EPR Active Probe. Biochemistry. 1992; 32: 1774.

C. Mailer, D.A. Haas, E.J. Hustedt, J.G. Gladden, and B.H. Robinson. Low-Power Electron Paramagnetic Resonance Spin-Echo Spectroscopy. J Mag Res. 1991; 91: 475.

E.J. Hustedt, H. Thomann, and B.H. Robinson. A Theoretical Treatment of Multiple Quantum Nuclear Spin Coherence in Electron Spin Echo Studies of Polyacetylene. J Chem Phys. 1990; 92: 978.

R. St. Denis, E.J. Hustedt, C. Mailer, and B.H. Robinson. Spin-lattice Relaxation of the Solitonic Defect in Isotopically Substituted Trans-polyacetylene. J. Phys. Chem. 1990; 94: 4716.

J.J. Kirchner, E.J. Hustedt, B.H. Robinson, and P.B. Hopkins. DNA Dynamics from a Spin Probe: Dependence of Probe Motion on Tether Length. Tet. Lett. 1990; 31: 593.

C. Mailer, R. St. Denis, E.J. Hustedt, and B.H. Robinson. Spin Dynamics of Polyacetylene Using Magnetic Resonance. ┬┐Electron Paramagnetic Resonance of the Solid State┬┐, J. Weil editor, Canandian Institute of Chemistry, Saskatoon, Saskatchewan, Canada. 1987.