Brian E. Wadzinski, Ph.D.

Associate Professor

brian.wadzinski@vanderbilt.edu

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Faculty Appointments
Associate Professor of Pharmacology
Education
Ph.D., Pharmacology, University of Wisconsin, Madison, WisconsinB.Sc., Chemistry/Biochemistry, University of Wisconsin, Madison, Wisconsin
Office Address
424-B Robinson Research Building
23rd Avenue South at Pierce
Nashville, TN 37232-6600
Research Description
The integrated actions of both protein kinases and phosphatases govern the phosphorylation state of key intracellular proteins which are crucial for normal cellular function. Although less attention has been given to the protein serine/threonine phosphatases than to the serine/threonine kinases, it is nonetheless clear that this group of proteins plays an integral role in the control of cell growth and differentiation. Our overall research program is the study of cellular protein serine/threonine phosphatases (PPs); however, our studies have focused on PP2A-like enzymes. This family of holoenzymes has been implicated in many facets of cellular function including regulation of metabolic enzymes, cell-surface receptors, cytosolic protein kinases, and transcription factors. Moreover, PP2A itself is regulated in many ways which include association with regulatory subunits, activation or inactivation by associated proteins, and post-translational modifications. We are using multiple complementary approaches including biochemical, immunological, and molecular biology techniques to determine the structure, function, cellular localization, and regulation of PP2A-like enzymes. Particular emphasis has been placed on studying PP2A multi-protein complexes as a first step towards understanding in molecular detail the way phosphorylation/dephosphorylation reactions control cell signalling pathways. Our studies have revealed the existence of protein kinase/PP2A signaling modules as a new paradigm for the control of various intracellular signaling cascades.
Research Keywords
Structure, function, and regulation of protein serine/threonine phosphatases
Publications
McConnell JL, Wadzinski BE. Targeting protein serine/threonine phosphatases for drug development. Mol. Pharmacol [print-electronic]. 2009 Jun; 75(6): 1249-61. PMID: 19299564, PMCID: PMC2684880, PII: mol.108.053140, DOI: 10.1124/mol.108.053140, ISSN: 1521-0111.

Guo S, Burnette R, Zhao L, Vanderford NL, Poitout V, Hagman DK, Henderson E, Ozcan S, Wadzinski BE, Stein R. The stability and transactivation potential of the mammalian MafA transcription factor are regulated by serine 65 phosphorylation. J. Biol. Chem [print-electronic]. 2009 Jan 1/9/2009; 284(2): 759-65. PMID: 19004825, PMCID: PMC2613637, PII: M806314200, DOI: 10.1074/jbc.M806314200, ISSN: 0021-9258.

Wang N, Leung HT, Pak WL, Carl YT, Wadzinski BE, Shieh BH. Role of protein phosphatase 2A in regulating the visual signaling in Drosophila. J. Neurosci. 2008 Feb 2/6/2008; 28(6): 1444-51. PMID: 18256265, PMCID: PMC2275662, PII: 28/6/1444, DOI: 10.1523/JNEUROSCI.5134-07.2008, ISSN: 1529-2401.

McConnell JL, Gomez RJ, McCorvey LR, Law BK, Wadzinski BE. Identification of a PP2A-interacting protein that functions as a negative regulator of phosphatase activity in the ATM/ATR signaling pathway. Oncogene [print-electronic]. 2007 Sep 9/6/2007; 26(41): 6021-30. PMID: 17384681, PII: 1210406, DOI: 10.1038/sj.onc.1210406, ISSN: 0950-9232.

Adams DG, Wadzinski BE. Isolation and characterization of PP2A holoenzymes containing FLAG-tagged B subunits. Methods Mol. Biol. 2007; 365: 101-11. PMID: 17200557, PII: 1-59745-267-X:101, DOI: 10.1385/1-59745-267-X:101, ISSN: 1064-3745.

Wegner AM, McConnell JL, Blakely RD, Wadzinski BE. An automated fluorescence-based method for continuous assay of PP2A activity. Methods Mol. Biol. 2007; 365: 61-9. PMID: 17200554, PII: 1-59745-267-X:61, DOI: 10.1385/1-59745-267-X:61, ISSN: 1064-3745.

Khoo MS, Li J, Singh MV, Yang Y, Kannankeril P, Wu Y, Grueter CE, Guan X, Oddis CV, Zhang R, Mendes L, Ni G, Madu EC, Yang J, Bass M, Gomez RJ, Wadzinski BE, Olson EN, Colbran RJ, Anderson ME. Death, cardiac dysfunction, and arrhythmias are increased by calmodulin kinase II in calcineurin cardiomyopathy. Circulation [print-electronic]. 2006 Sep 9/26/2006; 114(13): 1352-9. PMID: 16982937, PII: CIRCULATIONAHA.106.644583, DOI: 10.1161/CIRCULATIONAHA.106.644583, ISSN: 1524-4539.

Codreanu SG, Adams DG, Dawson ES, Wadzinski BE, Liebler DC. Inhibition of protein phosphatase 2A activity by selective electrophile alkylation damage. Biochemistry. 2006 Aug 8/22/2006; 45(33): 10020-9. PMID: 16906760, DOI: 10.1021/bi060551n, ISSN: 0006-2960.

Veluthakal R, Wadzinski BE, Kowluru A. Localization of a nuclear serine/threonine protein phosphatase in insulin-secreting INS-1 cells: potential regulation by IL-1beta. Apoptosis. 2006 Aug; 11(8): 1401-11. PMID: 16830232, DOI: 10.1007/s10495-006-8371-1, ISSN: 1360-8185.

Bridges D, MacDonald JA, Wadzinski B, Moorhead GB. Identification and characterization of D-AKAP1 as a major adipocyte PKA and PP1 binding protein. Biochem. Biophys. Res. Commun [print-electronic]. 2006 Jul 7/21/2006; 346(1): 351-7. PMID: 16756943, PII: S0006-291X(06)01210-1, DOI: 10.1016/j.bbrc.2006.05.138, ISSN: 0006-291X.

Fritz A, Brayer KJ, McCormick N, Adams DG, Wadzinski BE, Vaillancourt RR. Phosphorylation of serine 526 is required for MEKK3 activity, and association with 14-3-3 blocks dephosphorylation. J. Biol. Chem [print-electronic]. 2006 Mar 3/10/2006; 281(10): 6236-45. PMID: 16407301, PII: M509249200, DOI: 10.1074/jbc.M509249200, ISSN: 0021-9258.

Adams DG, Coffee RL, Zhang H, Pelech S, Strack S, Wadzinski BE. Positive regulation of Raf1-MEK1/2-ERK1/2 signaling by protein serine/threonine phosphatase 2A holoenzymes. J. Biol. Chem [print-electronic]. 2005 Dec 12/30/2005; 280(52): 42644-54. PMID: 16239230, PII: M502464200, DOI: 10.1074/jbc.M502464200, ISSN: 0021-9258.

Kray AE, Carter RS, Pennington KN, Gomez RJ, Sanders LE, Llanes JM, Khan WN, Ballard DW, Wadzinski BE. Positive regulation of IkappaB kinase signaling by protein serine/threonine phosphatase 2A. J. Biol. Chem [print-electronic]. 2005 Oct 10/28/2005; 280(43): 35974-82. PMID: 16126728, PII: M506093200, DOI: 10.1074/jbc.M506093200, ISSN: 0021-9258.

Van Kanegan MJ, Adams DG, Wadzinski BE, Strack S. Distinct protein phosphatase 2A heterotrimers modulate growth factor signaling to extracellular signal-regulated kinases and Akt. J. Biol. Chem [print-electronic]. 2005 Oct 10/28/2005; 280(43): 36029-36. PMID: 16129692, PII: M506986200, DOI: 10.1074/jbc.M506986200, ISSN: 0021-9258.

Zhang X, Ozawa Y, Lee H, Wen YD, Tan TH, Wadzinski BE, Seto E. Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4. Genes Dev. 2005 Apr 4/1/2005; 19(7): 827-39. PMID: 15805470, PMCID: PMC1074320, PII: 19/7/827, DOI: 10.1101/gad.1286005, ISSN: 0890-9369.

Garriga J, Jayaraman AL, Limón A, Jayadeva G, Sotillo E, Truongcao M, Patsialou A, Wadzinski BE, Graña X. A dynamic equilibrium between CDKs and PP2A modulates phosphorylation of pRB, p107 and p130. Cell Cycle [print-electronic]. 2004 Oct; 3(10): 1320-30. PMID: 15467457, PII: 1183, ISSN: 1551-4005.

Wells L, Kreppel LK, Comer FI, Wadzinski BE, Hart GW. O-GlcNAc transferase is in a functional complex with protein phosphatase 1 catalytic subunits. J. Biol. Chem [print-electronic]. 2004 Sep 9/10/2004; 279(37): 38466-70. PMID: 15247246, PII: M406481200, DOI: 10.1074/jbc.M406481200, ISSN: 0021-9258.

Anderson KA, Noeldner PK, Reece K, Wadzinski BE, Means AR. Regulation and function of the calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2A signaling complex. J. Biol. Chem [print-electronic]. 2004 Jul 7/23/2004; 279(30): 31708-16. PMID: 15143065, PII: M404523200, DOI: 10.1074/jbc.M404523200, ISSN: 0021-9258.

Anderson, K.A., Noeldner, P.K., Reece, K., Wadzinski, B.E., Means, A.R.. Regulation and function of the calcium/calmodulin-dependent protein kinase IV/protein serinethreonine phosphatase 2A signaling complex. J.Biol. Chem. 2004; 279: 31708-16.

Wells, L., Kreppel, L.K., Comer, F.I., Wadzinski, B.E., Hart, G.W.. O-GlcNAc transferase is in a functional complex with protein phosphatase 1 catalytic subunits. J. Biol. Chem. 2004.

Garriga, J., Jayaraman, A.L., Limon, A., Jayadeva, G., Sotillo, E., Truongcao, M., Patsialou, A., Wadzinski, B.E., Grana, X.. Cell Cycle [ in press ]. 2004.

Kloeker S, Reed R, McConnell JL, Chang D, Tran K, Westphal RS, Law BK, Colbran RJ, Kamoun M, Campbell KS, Wadzinski BE. Parallel purification of three catalytic subunits of the protein serine/threonine phosphatase 2A family (PP2A(C), PP4(C), and PP6(C)) and analysis of the interaction of PP2A(C) with alpha4 protein. Protein Expr. Purif. 2003 Sep; 31(1): 19-33. PMID: 12963337, PII: S1046592803001414, ISSN: 1046-5928.

Dagda RK, Zaucha JA, Wadzinski BE, Strack S. A developmentally regulated, neuron-specific splice variant of the variable subunit Bbeta targets protein phosphatase 2A to mitochondria and modulates apoptosis. J. Biol. Chem [print-electronic]. 2003 Jul 7/4/2003; 278(27): 24976-85. PMID: 12716901, PII: M302832200, DOI: 10.1074/jbc.M302832200, ISSN: 0021-9258.

Kabashima T, Kawaguchi T, Wadzinski BE, Uyeda K. Xylulose 5-phosphate mediates glucose-induced lipogenesis by xylulose 5-phosphate-activated protein phosphatase in rat liver. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2003 Apr 4/29/2003; 100(9): 5107-12. PMID: 12684532, PMCID: PMC154306, PII: 0730817100, DOI: 10.1073/pnas.0730817100, ISSN: 0027-8424.

Colbran RJ, Carmody LC, Bauman PA, Wadzinski BE, Bass MA. Analysis of specific interactions of native protein phosphatase 1 isoforms with targeting subunits. Meth. Enzymol. 2003; 366: 156-75. PMID: 14674248, ISSN: 0076-6879.

Strayhorn WD, Wadzinski BE. A novel in vitro assay for deubiquitination of I kappa B alpha. Arch. Biochem. Biophys. 2002 Apr 4/1/2002; 400(1): 76-84. PMID: 11913973, PII: S0003986102927608, DOI: 10.1006/abbi.2002.2760, ISSN: 0003-9861.

Strayhorn, W.D., Wadzinski, B.E.. A novel in vitro assay for deubiquitination of IkB??. Arch. Biochem. Biophys. 2002; 400: 76-84.

Yang J, Fan GH, Wadzinski BE, Sakurai H, Richmond A. Protein phosphatase 2A interacts with and directly dephosphorylates RelA. J. Biol. Chem [print-electronic]. 2001 Dec 12/21/2001; 276(51): 47828-33. PMID: 11591705, PMCID: PMC2665282, PII: M106103200, DOI: 10.1074/jbc.M106103200, ISSN: 0021-9258.

Wada T, Miyata T, Inagi R, Nangaku M, Wagatsuma M, Suzuki D, Wadzinski BE, Okubo K, Kurokawa K. Cloning and characterization of a novel subunit of protein serine/threonine phosphatase 4 from mesangial cells. J. Am. Soc. Nephrol. 2001 Dec; 12(12): 2601-8. PMID: 11729228, ISSN: 1046-6673.

Chiang CW, Harris G, Ellig C, Masters SC, Subramanian R, Shenolikar S, Wadzinski BE, Yang E. Protein phosphatase 2A activates the proapoptotic function of BAD in interleukin- 3-dependent lymphoid cells by a mechanism requiring 14-3-3 dissociation. Blood. 2001 Mar 3/1/2001; 97(5): 1289-97. PMID: 11222372, ISSN: 0006-4971.

Chiang, C.-W., Harris, G., Ellig, C., Masters, S.C., Subramanian, R., Shenolikar, S., Wadzinski, B.E., and Yang, E. Protein phosphatase 2A activates the proapoptotic function of BAD in interleukin-3-dependent lymphoid cells by a mechanism requiring 14-3-3 dissociation. Blood. 2001; 97: 1289-97.

Wada, T., Miyata, T., Inagi, R., Nangaku, M., Wagatsuma, M., Suzuki, D., Wadzinski, B.E., Okubo, K., and Kurokawa, K. Cloning and characterization of a novel subunit of protein serine/threonine phosphatase 4 from mesangial cells. J. Am. Soc. Nephrol. 2001.

Bauman AL, Apparsundaram S, Ramamoorthy S, Wadzinski BE, Vaughan RA, Blakely RD. Cocaine and antidepressant-sensitive biogenic amine transporters exist in regulated complexes with protein phosphatase 2A. J. Neurosci. 2000 Oct 10/15/2000; 20(20): 7571-8. PMID: 11027216, PII: 20/20/7571, ISSN: 1529-2401.

Liu M, Parker LL, Wadzinski BE, Shieh BH. Reversible phosphorylation of the signal transduction complex in Drosophila photoreceptors. J. Biol. Chem. 2000 Apr 4/21/2000; 275(16): 12194-9. PMID: 10766855, ISSN: 0021-9258.

Liu M, Parker LL, Wadzinski BE and Shieh BH. Reversible phosphorylation of the signal transduction complex in Drosophila photoreceptors. J Biol Chem. 2000; 275((16)): 12194-9.

Bauman, A.L., Apparsundaram, S., Ramamoorthy, S., Wadzinski, B.E., Vaughan, R.A., and Blakely, R.D. Cocaine and antidepressant-sensitive biogenic amine transporters exist in regulated complexes with protein phosphatase 2A. J. Neurosci. 2000; 20: 7571-8.

MacMillan LB, Bass MA, Cheng N, Howard EF, Tamura M, Strack S, Wadzinski BE, Colbran RJ. Brain actin-associated protein phosphatase 1 holoenzymes containing spinophilin, neurabin, and selected catalytic subunit isoforms. J. Biol. Chem. 1999 Dec 12/10/1999; 274(50): 35845-54. PMID: 10585469, ISSN: 0021-9258.

Strack S, Chang D, Zaucha JA, Colbran RJ, Wadzinski BE. Cloning and characterization of B delta, a novel regulatory subunit of protein phosphatase 2A. FEBS Lett. 1999 Nov 11/5/1999; 460(3): 462-6. PMID: 10556517, PII: S0014-5793(99)01377-0, ISSN: 0014-5793.

Strack S, Kini S, Ebner FF, Wadzinski BE, Colbran RJ. Differential cellular and subcellular localization of protein phosphatase 1 isoforms in brain. J. Comp. Neurol. 1999 Oct 10/25/1999; 413(3): 373-84. PMID: 10502246, PII: 10.1002/(SICI)1096-9861(19991025)413:3<373::AID-CNE2>3.0.CO;2-Z, ISSN: 0021-9967.

Bryant JC, Westphal RS, Wadzinski BE. Methylated C-terminal leucine residue of PP2A catalytic subunit is important for binding of regulatory Balpha subunit. Biochem. J. 1999 Apr 4/15/1999; 339 ( Pt 2): 241-6. PMID: 10191253, PMCID: PMC1220151, ISSN: 0264-6021.

Kloeker S, Wadzinski BE. Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. J. Biol. Chem. 1999 Feb 2/26/1999; 274(9): 5339-47. PMID: 10026142, ISSN: 0021-9258.

Westphal RS, Coffee RL, Marotta A, Pelech SL, Wadzinski BE. Identification of kinase-phosphatase signaling modules composed of p70 S6 kinase-protein phosphatase 2A (PP2A) and p21-activated kinase-PP2A. J. Biol. Chem. 1999 Jan 1/8/1999; 274(2): 687-92. PMID: 9873003, ISSN: 0021-9258.

Bryant JC, Westphal RS and Wadzinski BE. Methylated C- terminal leucine residue of PP2A catalytic subunit is important for binding of regulatory B-alpha subunit. Biochem J. 1999; 339((2)): 241-6.

Strack S, Kini S, Ebner FF, Wadzinski BE and Colbran RJ. Differential cellular and subcellular localization of protein phosphatase 1 isoforms in brain. J Comp Neurol. 1999; 413((3)): 373-84.

Westphal, R.S., Coffee, R.L., Jr., Morratta, T., Pelech, S., and Wadzinski, B.E. Identification of Kinase/Phosphatase Signaling Modules Composed of p70S6 Kinase-Protein Phosphatase 2A (PP2A) and p21-Activated Kinase-PP2A. J. Biol.Chem. 1999; 274: 687-92.

Kloeker, S. and Wadzinski, B.E. Purification and Identification of a Novel Subunit of Protein Serine/Threonine Phosphatase 4. J. Biol. Chem. 1999; 274: 5339-47.

Filali, M., Li, S., Kim, H.W., Wadzinski, B.E., and Kamoun, M. Identification of a Type 6 Protein Ser/Thr Phosphatase Regulated by Interleukin-2 Stimulation. J. Cell. Biochem. 1999; 73: 153-63.

Strack, S., Chang, D., Zaucha, J.A., Colbran, R.J., and Wadzinski, B.E. Cloning and Characterization of Ba Novel Regulatory Subunit of Protein Phosphatase 2A. FEBS Lett. 1999; 460: 462-6.

Price, N.E., Wadzinski, B.E., and Mumby, M.C. An Anchoring Factor Targets Protein Phosphatase 2A to Brain Microtubules. Mol. Brain Res. 1999; 73: 68.

MacMillan, L.B., Bass, M.A., Cheng, N., Howard, E.F., Tamura, M., Strack, S., Wadzinski, B.E., and Colbran, R.J. Brain Actin-Associated Protein Phosphatase 1 Holoenzyme Containing Spinophilin, Neurabin and Selected Catalytic Subunit Isoforms. J. Biol. Chem. 1999; 274: 35845-54.

Filali, M., li, S., Kim, H.W., Wadzinski, B.E., Kamoun, M.. Identification of a type 6 protein ser/thr phosphatase regulated by interleukin-2 stimulation. J Cell Biochem. 1999; 73: 153-63.

Price, N.E, Wadzinski, B.E., Mumby, M.C.. An anchoring factor targets protein phosphatase 2A to brain microtubules. Mol. Brain Res. 1999; 73: 68-77.

Westphal RS, Anderson KA, Means AR, Wadzinski BE. A signaling complex of Ca2+-calmodulin-dependent protein kinase IV and protein phosphatase 2A. Science. 1998 May 5/22/1998; 280(5367): 1258-61. PMID: 9596578, ISSN: 0036-8075.

Strack S, Zaucha JA, Ebner FF, Colbran RJ, Wadzinski BE. Brain protein phosphatase 2A: developmental regulation and distinct cellular and subcellular localization by B subunits. J. Comp. Neurol. 1998 Mar 3/23/1998; 392(4): 515-27. PMID: 9514514, PII: 10.1002/(SICI)1096-9861(19980323)392:4<515::AID-CNE8>3.0.CO;2-3, ISSN: 0021-9967.

Zaucha JA, Westphal RS, Wadzinski BE. Protein phosphatase 2A regulatory subunits. cDNA cloning and analysis of mRNA expression. Methods Mol. Biol. 1998; 93: 279-91. PMID: 9664545, DOI: 10.1385/0-89603-468-2:279, ISSN: 1064-3745.

Strack S, Zaucha JA, Ebner FF, Colbran RJ and Wadzinski BE. Brain protein phosphatase 2A: developmental regulation and distinct cellular and subcellular localization by B subunits. J Comp Neurol. 1998; 392: 515-27.

Zaucha JA, Westphal RS and Wadzinski BE. Protein Phosphatase 2A Regulatory Subunits: cDNA Cloning and Analysis of mRNA Expression. Methods in Molecular Biology. 1998; 93: 279-91.

Westphal RS, Anderson KA, Means AR and Wadzinski BE. A signaling complex of Ca2+/calmodulin-dependent protein kinase IV and protein phosphatase 2A. Science. 1998; 280: 1258-60.

Kloeker S, Bryant JC, Strack S, Colbran RJ, Wadzinski BE. Carboxymethylation of nuclear protein serine/threonine phosphatase X. Biochem. J. 1997 Oct 10/15/1997; 327 ( Pt 2): 481-6. PMID: 9359419, PMCID: PMC1218819, ISSN: 0264-6021.

Strack S, Westphal RS, Colbran RJ, Ebner FF, Wadzinski BE. Protein serine/threonine phosphatase 1 and 2A associate with and dephosphorylate neurofilaments. Brain Res. Mol. Brain Res. 1997 Oct 10/3/1997; 49(1-2): 15-28. PMID: 9387859, ISSN: 0169-328X.

Colbran RJ, Bass MA, McNeill RB, Bollen M, Zhao S, Wadzinski BE, Strack S. Association of brain protein phosphatase 1 with cytoskeletal targeting/regulatory subunits. J. Neurochem. 1997 Sep; 69(3): 920-9. PMID: 9282913, ISSN: 0022-3042.

Strack S, Barban MA, Wadzinski BE, Colbran RJ. Differential inactivation of postsynaptic density-associated and soluble Ca2+/calmodulin-dependent protein kinase II by protein phosphatases 1 and 2A. J. Neurochem. 1997 May; 68(5): 2119-28. PMID: 9109540, ISSN: 0022-3042.

Strack S, Westphal RS, Colbran RJ, Ebner FF and Wadzinski BE. Protein serine/threonine phosphatase 1 and 2A associate with and dephosphorylate neurofilaments. Mol Brain Res. 1997; 49: 15-28.

Strack, S., Barban, M.A., Wadzinski, B.E., and Colbran, R.J. Differential Inactivation of Postsynaptic Density-Associated and Soluble Ca+2/Calmodulin-Dependent Protein Kinase II by Protein Phosphatases 1 and 2A. J. Neurochem. 1997; 68: 2119-28.

Colbran, R.J., Bass, M.A., McNeill, R.B., Bollen, M., Zhao, S., Wadzinski, B.E., and Strack, S. Association of Brain Protein Phosphatase 1 with Cytoskeletal Targeting/Regulatory Subunits. J. Neurochem. 1997; 69: 920-9.

Kloeker, S., Bryant, J.C., Strack, S., Colbran, R.J., and Wadzinski, B.E. Carboxymethylation of Nuclear Protein Serine/Threonine Phosphatase X. Biochem. J. 1997; 327: 481-6.

Strack S, Wadzinski BE, Ebner FF. Localization of the calcium/calmodulin-dependent protein phosphatase, calcineurin, in the hindbrain and spinal cord of the rat. J. Comp. Neurol. 1996 Nov 11/4/1996; 375(1): 66-76. PMID: 8913893, PII: 10.1002/(SICI)1096-9861(19961104)375:1<66::AID-CNE4>3.0.CO;2-M, DOI: 10.1002/(SICI)1096-9861(19961104)375:1<66::AID-CNE4>3.0.CO;2-M, ISSN: 0021-9967.

Strack, S., Wadzinski, B.E., and Ebner, F.F. Localization of Calcium/Calmodulin-Dependent Protein Phosphatase, Calcineurin, in the Hindbrain and Spinal Cord of the Rat. J. Comp. Neurol. 1996; 375: 66-76.

Wadzinski BE, Wheat WH, Jaspers S, Peruski LF, Lickteig RL, Johnson GL, Klemm DJ. Nuclear protein phosphatase 2A dephosphorylates protein kinase A-phosphorylated CREB and regulates CREB transcriptional stimulation. Mol. Cell. Biol. 1993 May; 13(5): 2822-34. PMID: 8386317, PMCID: PMC359667, ISSN: 0270-7306.

Wadzinski, B.E., Wheat, W.H., Jasper, S., Peruski, L.F., Jr., Lickteig, R.L., Johnson, G.L., and Klemm, D.J. Nuclear Protein Phosphatase 2A Dephosphorylates Protein Kinase A-Phosphorylated CREB and Regulates CREB Transcriptional Stimulation. Mol. Cell. Biol. 1993; 13: 2822-34.

Peruski, L.F., Jr., Wadzinski, B.E., and Johnson, G.L. Analysis of the Multiplicity, Structure, and Function of Protein Serine/Threonine Phosphatases. Advances in Protein Phosphatases. 1993; 7: 9-30.

Wadzinski BE, Eisfelder BJ, Peruski LF, Mumby MC, Johnson GL. NH2-terminal modification of the phosphatase 2A catalytic subunit allows functional expression in mammalian cells. J. Biol. Chem. 1992 Aug 8/25/1992; 267(24): 16883-8. PMID: 1380955, ISSN: 0021-9258.

Wadzinski, B.E., Eisfelder, B.J., Peruski, L.F., Jr., Mumby, M.C., and Johnson, G.L. NH2-terminal Modification of the Phosphatase 2A Catalytic Subunit Allows Functional Expression in Mammalian Cells. J. Biol. Chem. 1992; 267: 16883-8.

Devaskar S, Zahm DS, Holtzclaw L, Chundu K, Wadzinski BE. Developmental regulation of the distribution of rat brain insulin-insensitive (Glut 1) glucose transporter. Endocrinology. 1991 Sep; 129(3): 1530-40. PMID: 1874186, DOI: 10.1210/endo-129-3-1530, ISSN: 0013-7227.

Devaskar, S., Zahm, D.S., Holtzclaw, L., Chundu, K., and Wadzinski, B.E. Developmental Regulation of the Distribution of Rat Brain Insulin-Insensitive (Glut1) Glucose Transporter. Endocrinology. 1991; 129: 1530-40.

Wadzinski BE, Heasley LE, Johnson GL. Multiplicity of protein serine-threonine phosphatases in PC12 pheochromocytoma and FTO-2B hepatoma cells. J. Biol. Chem. 1990 Dec 12/15/1990; 265(35): 21504-8. PMID: 2174876, ISSN: 0021-9258.

Wadzinski BE, Shanahan MF, Seamon KB, Ruoho AE. Localization of the forskolin photolabelling site within the monosaccharide transporter of human erythrocytes. Biochem. J. 1990 Nov 11/15/1990; 272(1): 151-8. PMID: 2264820, PMCID: PMC1149670, ISSN: 0264-6021.

Wadzinski, B.E., Shanahan, M.F., Seamon, K.B., and Ruoho, A.E. Localization of the Forskolin Photolabelling Site Within the Monosaccharide Transporter of Human Erythrocytes. Biochem. J. 1990; 272: 151-8.

Wadzinski, B.E., Heasley, L.E., and Johnson, G.L. Multiplicity of Protein Serine-Threonine Phosphatases in PC12 Pheochromocytoma and FTO-2B Hepatoma Cells. J. Biol. Chem. 1990; 265: 21504-8.

Wadzinski BE, Shanahan MF, Clark RB, Ruoho AE. Identification of the glucose transporter in mammalian cell membranes with a 125I-forskolin photoaffinity label. Biochem. J. 1988 Nov 11/1/1988; 255(3): 983-90. PMID: 3063259, PMCID: PMC1135338, ISSN: 0264-6021.

Wadzinski, B.E., Shanahan, M.F., Clark, R.B., and Ruoho, A.E. Identification of the Glucose Transporter in Mammalian Cell Membranes with a 125I-Forskolin Photoaffinity Label. Biochem. J. 1988; 255: 983-90.

Wadzinski BE, Shanahan MF, Ruoho AE. Derivatization of the human erythrocyte glucose transporter using a novel forskolin photoaffinity label. J. Biol. Chem. 1987 Dec 12/25/1987; 262(36): 17683-9. PMID: 3693367, ISSN: 0021-9258.

Shanahan MF, Wadzinski BE, Lowndes JM, Ruoho AE. Photoaffinity labeling of the human erythrocyte monosaccharide transporter with an aryl azide derivative of D-glucose. J. Biol. Chem. 1985 Sep 9/15/1985; 260(20): 10897-900. PMID: 4040910, ISSN: 0021-9258.

Zorzano, A., Wilkinson, W., Kotliar, N., Thoidis, G., Wadzinski, B.E., Ruoho, A.E., and Pilch, P.F. Insulin-regulated Glucose Uptake in Rat Adipocytes is Mediated by Two Transporter Isoforms Present in at Least Two Vesicle Populations. J. Biol. Chem. 264: 12358-63.