Vsevolod V. Gurevich, Ph.D.



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Faculty Appointments
Professor of Pharmacology
Ph.D., Shemyakin Institute, Moscow, RussiaM.S., Moscow State University, Moscow, RussiaB.S., Moscow State University, Moscow, Russia
Office Address
417D PRB
2200 Pierce Avenue
Nashville, TN 37232-6600
Research Description
We are interested in structure, function, and biology of arrestin proteins. Arrestins bind activated phosphorylated G protein-coupled receptors (GPCRs), thereby shutting down their signaling via G proteins (desensitization), targeting receptors for internalization. Free and receptor-bound arrestins are multi-functional signaling adapters, interacting with more than 20 signaling proteins, many of which play key role in "life-or-death" decisions in the cell. We want to understand the molecular mechanisms that arrestins use to "decide" when to bind particular interaction partners and when to dissociate. We have mapped receptor-arrestin interaction interface fairly well and we want to elucidate arrestin binding sites for its other interaction partners with the same precision. We intend to use this information to construct "custom-designed" arrestins that link the receptor of interest to the signaling pathway of our choosing. These tools will allow us to tell the cell what to do and when to do it. For example, "biased" arrestins can enhance pro-survival signaling, preventing cell death characteristic for neurodegenerative diseases (such as Alzheimer's, Parkinson's, or retinal degeneration), or tip the balance toward cell death, which would be useful to prevent uncontrolled proliferation characteristic for cancer.
The solution of the crystal structure of three arrestin proteins and elucidation of the mechanism of arrestin phosphate sensor action allowed us to construct arrestin mutants that bind the active form of their cognate GPCRs regardless of receptor phosphorylation. These "super-arrestins" may prove useful for gene therapy of disorders associated with excessive signaling by various GPCRs that range from night blindness and retinal degeneration to several forms of cancer. We are testing whether transgenic expression of phosphorylation-independent mutants of visual arrestin prevents retinal degeneration in several mouse models.
We believe that the combination of different approaches ranging from hard-core biochemical and biophysical methods and X-ray crystallography to cell culture and transgenic animals is necessary to answer biologically relevant questions concerning various facets of arrestin function and to create novel therapeutic tools based on this information.
Research Keywords
Arrestins Structure-function G protein-coupled receptors Vision Signal transduction Trafficking Neurodevelopment Transgenic animal models Cell survival
Song X, Seo J, Baameur F, Vishnivetskiy SA, Chen Q, Kook S, Kim M, Brooks EK, Altenbach C, Hong Y, Hanson SM, Palazzo MC, Chen J, Hubbell WL, Gurevich EV, Gurevich VV. Rapid degeneration of rod photoreceptors expressing self-association-deficient arrestin-1 mutant. Cell. Signal [print-electronic]. 2013 Dec; 25(12): 2613-24. PMID: 24012956, PMCID: PMC3833262, PII: S0898-6568(13)00257-X, DOI: 10.1016/j.cellsig.2013.08.022, ISSN: 1873-3913.

Vishnivetskiy SA, Ostermaier MK, Singhal A, Panneels V, Homan KT, Glukhova A, Sligar SG, Tesmer JJ, Schertler GF, Standfuss J, Gurevich VV. Constitutively active rhodopsin mutants causing night blindness are effectively phosphorylated by GRKs but differ in arrestin-1 binding. Cell. Signal [print-electronic]. 2013 Nov; 25(11): 2155-62. PMID: 23872075, PMCID: PMC3774132, PII: S0898-6568(13)00207-6, DOI: 10.1016/j.cellsig.2013.07.009, ISSN: 1873-3913.

Zhan X, Kaoud TS, Kook S, Dalby KN, Gurevich VV. JNK3 enzyme binding to arrestin-3 differentially affects the recruitment of upstream mitogen-activated protein (MAP) kinase kinases. J. Biol. Chem [print-electronic]. 2013 Oct 10/4/2013; 288(40): 28535-47. PMID: 23960075, PMCID: PMC3789954, PII: M113.508085, DOI: 10.1074/jbc.M113.508085, ISSN: 1083-351X.

Vishnivetskiy SA, Baameur F, Findley KR, Gurevich VV. Critical role of the central 139-loop in stability and binding selectivity of arrestin-1. J. Biol. Chem [print-electronic]. 2013 Apr 4/26/2013; 288(17): 11741-50. PMID: 23476014, PMCID: PMC3636863, PII: M113.450031, DOI: 10.1074/jbc.M113.450031, ISSN: 1083-351X.

Vishnivetskiy SA, Chen Q, Palazzo MC, Brooks EK, Altenbach C, Iverson TM, Hubbell WL, Gurevich VV. Engineering visual arrestin-1 with special functional characteristics. J. Biol. Chem [print-electronic]. 2013 Feb 2/1/2013; 288(5): 3394-405. PMID: 23250748, PMCID: PMC3561558, PII: M112.445437, DOI: 10.1074/jbc.M112.445437, ISSN: 1083-351X.

Zhuang T, Chen Q, Cho MK, Vishnivetskiy SA, Iverson TM, Gurevich VV, Sanders CR. Involvement of distinct arrestin-1 elements in binding to different functional forms of rhodopsin. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 Jan 1/15/2013; 110(3): 942-7. PMID: 23277586, PMCID: PMC3549108, PII: 1215176110, DOI: 10.1073/pnas.1215176110, ISSN: 1091-6490.

Gurevich VV, Gurevich EV. Structural determinants of arrestin functions. Prog Mol Biol Transl Sci. 2013; 118: 57-92. PMID: 23764050, PMCID: PMC4514030, PII: B978-0-12-394440-5.00003-6, DOI: 10.1016/B978-0-12-394440-5.00003-6, ISSN: 1878-0814.

Gurevich VV, Gurevich EV. Synthetic biology with surgical precision: targeted reengineering of signaling proteins. Cell. Signal [print-electronic]. 2012 Oct; 24(10): 1899-908. PMID: 22664341, PMCID: PMC3404258, PII: S0898-6568(12)00149-0, DOI: 10.1016/j.cellsig.2012.05.012, ISSN: 1873-3913.

Gimenez LE, Vishnivetskiy SA, Baameur F, Gurevich VV. Manipulation of very few receptor discriminator residues greatly enhances receptor specificity of non-visual arrestins. J. Biol. Chem [print-electronic]. 2012 Aug 8/24/2012; 287(35): 29495-505. PMID: 22787152, PMCID: PMC3436164, PII: M112.366674, DOI: 10.1074/jbc.M112.366674, ISSN: 1083-351X.

Breitman M, Kook S, Gimenez LE, Lizama BN, Palazzo MC, Gurevich EV, Gurevich VV. Silent scaffolds: inhibition OF c-Jun N-terminal kinase 3 activity in cell by dominant-negative arrestin-3 mutant. J. Biol. Chem [print-electronic]. 2012 Jun 6/1/2012; 287(23): 19653-64. PMID: 22523077, PMCID: PMC3366000, PII: M112.358192, DOI: 10.1074/jbc.M112.358192, ISSN: 1083-351X.

Gimenez LE, Kook S, Vishnivetskiy SA, Ahmed MR, Gurevich EV, Gurevich VV. Role of receptor-attached phosphates in binding of visual and non-visual arrestins to G protein-coupled receptors. J. Biol. Chem [print-electronic]. 2012 Mar 3/16/2012; 287(12): 9028-40. PMID: 22275358, PMCID: PMC3308753, PII: M111.311803, DOI: 10.1074/jbc.M111.311803, ISSN: 1083-351X.

Aguila B, Coulbault L, Davis A, Marie N, Hasbi A, Le bras F, Tóth G, Borsodi A, Gurevich VV, Jauzac P, Allouche S. ßarrestin1-biased agonism at human d-opioid receptor by peptidic and alkaloid ligands. Cell. Signal [print-electronic]. 2012 Mar; 24(3): 699-707. PMID: 22101011, PMCID: PMC3392209, PII: S0898-6568(11)00349-4, DOI: 10.1016/j.cellsig.2011.10.018, ISSN: 1873-3913.

Gurevich EV, Tesmer JJ, Mushegian A, Gurevich VV. G protein-coupled receptor kinases: more than just kinases and not only for GPCRs. Pharmacol. Ther [print-electronic]. 2012 Jan; 133(1): 40-69. PMID: 21903131, PMCID: PMC3241883, PII: S0163-7258(11)00165-3, DOI: 10.1016/j.pharmthera.2011.08.001, ISSN: 1879-016X.

Mushegian A, Gurevich VV, Gurevich EV. The origin and evolution of G protein-coupled receptor kinases. PLoS ONE [print-electronic]. 2012; 7(3): e33806. PMID: 22442725, PMCID: PMC3307776, PII: PONE-D-12-00358, DOI: 10.1371/journal.pone.0033806, ISSN: 1932-6203.

Zhan X, Kaoud TS, Dalby KN, Gurevich VV. Nonvisual arrestins function as simple scaffolds assembling the MKK4-JNK3a2 signaling complex. Biochemistry [print-electronic]. 2011 Dec 12/6/2011; 50(48): 10520-9. PMID: 22047447, PMCID: PMC3227541, DOI: 10.1021/bi201506g, ISSN: 1520-4995.

Gurevich VV, Hanson SM, Song X, Vishnivetskiy SA, Gurevich EV. The functional cycle of visual arrestins in photoreceptor cells. Prog Retin Eye Res [print-electronic]. 2011 Nov; 30(6): 405-30. PMID: 21824527, PMCID: PMC3196764, PII: S1350-9462(11)00052-8, DOI: 10.1016/j.preteyeres.2011.07.002, ISSN: 1873-1635.

Bychkov ER, Ahmed MR, Gurevich VV, Benovic JL, Gurevich EV. Reduced expression of G protein-coupled receptor kinases in schizophrenia but not in schizoaffective disorder. Neurobiol. Dis [print-electronic]. 2011 Nov; 44(2): 248-58. PMID: 21784156, PMCID: PMC3166984, PII: S0969-9961(11)00235-X, DOI: 10.1016/j.nbd.2011.07.009, ISSN: 1095-953X.

Coffa S, Breitman M, Spiller BW, Gurevich VV. A single mutation in arrestin-2 prevents ERK1/2 activation by reducing c-Raf1 binding. Biochemistry [print-electronic]. 2011 Aug 8/16/2011; 50(32): 6951-8. PMID: 21732673, PMCID: PMC3153575, DOI: 10.1021/bi200745k, ISSN: 1520-4995.

Seo J, Tsakem EL, Breitman M, Gurevich VV. Identification of arrestin-3-specific residues necessary for JNK3 kinase activation. J. Biol. Chem [print-electronic]. 2011 Aug 8/12/2011; 286(32): 27894-901. PMID: 21715332, PMCID: PMC3151035, PII: M111.260448, DOI: 10.1074/jbc.M111.260448, ISSN: 1083-351X.

Vishnivetskiy SA, Gimenez LE, Francis DJ, Hanson SM, Hubbell WL, Klug CS, Gurevich VV. Few residues within an extensive binding interface drive receptor interaction and determine the specificity of arrestin proteins. J. Biol. Chem [print-electronic]. 2011 Jul 7/8/2011; 286(27): 24288-99. PMID: 21471193, PMCID: PMC3129209, PII: M110.213835, DOI: 10.1074/jbc.M110.213835, ISSN: 1083-351X.

Caruso G, Bisegna P, Andreucci D, Lenoci L, Gurevich VV, Hamm HE, DiBenedetto E. Identification of key factors that reduce the variability of the single photon response. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2011 May 5/10/2011; 108(19): 7804-7. PMID: 21518901, PMCID: PMC3093507, PII: 1018960108, DOI: 10.1073/pnas.1018960108, ISSN: 1091-6490.

Ahmed MR, Zhan X, Song X, Kook S, Gurevich VV, Gurevich EV. Ubiquitin ligase parkin promotes Mdm2-arrestin interaction but inhibits arrestin ubiquitination. Biochemistry [print-electronic]. 2011 May 5/10/2011; 50(18): 3749-63. PMID: 21466165, PMCID: PMC3091828, DOI: 10.1021/bi200175q, ISSN: 1520-4995.

Kim M, Hanson SM, Vishnivetskiy SA, Song X, Cleghorn WM, Hubbell WL, Gurevich VV. Robust self-association is a common feature of mammalian visual arrestin-1. Biochemistry [print-electronic]. 2011 Mar 3/29/2011; 50(12): 2235-42. PMID: 21288033, PMCID: PMC3062689, DOI: 10.1021/bi1018607, ISSN: 1520-4995.

Zhan X, Gimenez LE, Gurevich VV, Spiller BW. Crystal structure of arrestin-3 reveals the basis of the difference in receptor binding between two non-visual subtypes. J. Mol. Biol [print-electronic]. 2011 Feb 2/25/2011; 406(3): 467-78. PMID: 21215759, PMCID: PMC3034793, PII: S0022-2836(10)01366-5, DOI: 10.1016/j.jmb.2010.12.034, ISSN: 1089-8638.

Song X, Vishnivetskiy SA, Seo J, Chen J, Gurevich EV, Gurevich VV. Arrestin-1 expression level in rods: balancing functional performance and photoreceptor health. Neuroscience [print-electronic]. 2011 Feb 2/3/2011; 174: 37-49. PMID: 21075174, PMCID: PMC3020241, PII: S0306-4522(10)01494-6, DOI: 10.1016/j.neuroscience.2010.11.009, ISSN: 1873-7544.

Bayburt TH, Vishnivetskiy SA, McLean MA, Morizumi T, Huang CC, Tesmer JJ, Ernst OP, Sligar SG, Gurevich VV. Monomeric rhodopsin is sufficient for normal rhodopsin kinase (GRK1) phosphorylation and arrestin-1 binding. J. Biol. Chem [print-electronic]. 2011 Jan 1/14/2011; 286(2): 1420-8. PMID: 20966068, PMCID: PMC3020750, PII: M110.151043, DOI: 10.1074/jbc.M110.151043, ISSN: 1083-351X.

Cleghorn WM, Tsakem EL, Song X, Vishnivetskiy SA, Seo J, Chen J, Gurevich EV, Gurevich VV. Progressive reduction of its expression in rods reveals two pools of arrestin-1 in the outer segment with different roles in photoresponse recovery. PLoS ONE [print-electronic]. 2011; 6(7): e22797. PMID: 21818392, PMCID: PMC3144249, PII: PONE-D-11-08272, DOI: 10.1371/journal.pone.0022797, ISSN: 1932-6203.

Coffa S, Breitman M, Hanson SM, Callaway K, Kook S, Dalby KN, Gurevich VV. The effect of arrestin conformation on the recruitment of c-Raf1, MEK1, and ERK1/2 activation. PLoS ONE [print-electronic]. 2011; 6(12): e28723. PMID: 22174878, PMCID: PMC3236217, PII: PONE-D-11-18971, DOI: 10.1371/journal.pone.0028723, ISSN: 1932-6203.

Walther C, Nagel S, Gimenez LE, Mörl K, Gurevich VV, Beck-Sickinger AG. Ligand-induced internalization and recycling of the human neuropeptide Y2 receptor is regulated by its carboxyl-terminal tail. J. Biol. Chem [print-electronic]. 2010 Dec 12/31/2010; 285(53): 41578-90. PMID: 20959467, PMCID: PMC3009885, PII: M110.162156, DOI: 10.1074/jbc.M110.162156, ISSN: 1083-351X.

Zhuang T, Vishnivetskiy SA, Gurevich VV, Sanders CR. Elucidation of inositol hexaphosphate and heparin interaction sites and conformational changes in arrestin-1 by solution nuclear magnetic resonance. Biochemistry [print-electronic]. 2010 Dec 12/14/2010; 49(49): 10473-85. PMID: 21050017, PMCID: PMC3074303, DOI: 10.1021/bi101596g, ISSN: 1520-4995.

Ahmed MR, Berthet A, Bychkov E, Porras G, Li Q, Bioulac BH, Carl YT, Bloch B, Kook S, Aubert I, Dovero S, Doudnikoff E, Gurevich VV, Gurevich EV, Bezard E. Lentiviral overexpression of GRK6 alleviates L-dopa-induced dyskinesia in experimental Parkinson's disease. Sci Transl Med. 2010 Apr 4/21/2010; 2(28): 28ra28. PMID: 20410529, PMCID: PMC2933751, PII: 2/28/28ra28, DOI: 10.1126/scitranslmed.3000664, ISSN: 1946-6242.

Shankar H, Michal A, Kern RC, Kang DS, Gurevich VV, Benovic JL. Non-visual arrestins are constitutively associated with the centrosome and regulate centrosome function. J. Biol. Chem [print-electronic]. 2010 Mar 3/12/2010; 285(11): 8316-29. PMID: 20056609, PMCID: PMC2832982, PII: M109.062521, DOI: 10.1074/jbc.M109.062521, ISSN: 1083-351X.

Vishnivetskiy SA, Francis D, Van Eps N, Kim M, Hanson SM, Klug CS, Hubbell WL, Gurevich VV. The role of arrestin alpha-helix I in receptor binding. J. Mol. Biol [print-electronic]. 2010 Jan 1/8/2010; 395(1): 42-54. PMID: 19883657, PMCID: PMC2787876, PII: S0022-2836(09)01317-5, DOI: 10.1016/j.jmb.2009.10.058, ISSN: 1089-8638.

Caruso G, Bisegna P, Lenoci L, Andreucci D, Gurevich VV, Hamm HE, DiBenedetto E. Kinetics of rhodopsin deactivation and its role in regulating recovery and reproducibility of rod photoresponse. PLoS Comput. Biol. 2010; 6(12): e1001031. PMID: 21200415, PMCID: PMC3002991, DOI: 10.1371/journal.pcbi.1001031, ISSN: 1553-7358.

Gurevich VV, Gurevich EV. Custom-designed proteins as novel therapeutic tools? The case of arrestins. Expert Rev Mol Med. 2010; 12: e13. PMID: 20412604, PMCID: PMC2933791, PII: S1462399410001444, DOI: 10.1017/S1462399410001444, ISSN: 1462-3994.

Shen L, Caruso G, Bisegna P, Andreucci D, Gurevich VV, Hamm HE, DiBenedetto E. Dynamics of mouse rod phototransduction and its sensitivity to variation of key parameters. IET Syst Biol. 2010 Jan; 4(1): 12-32. PMID: 20001089, PMCID: PMC3833298, DOI: 10.1049/iet-syb.2008.0154, ISSN: 1751-8849.

Song X, Vishnivetskiy SA, Gross OP, Emelianoff K, Mendez A, Chen J, Gurevich EV, Burns ME, Gurevich VV. Enhanced arrestin facilitates recovery and protects rods lacking rhodopsin phosphorylation. Curr. Biol [print-electronic]. 2009 Apr 4/28/2009; 19(8): 700-5. PMID: 19361994, PMCID: PMC2768495, PII: S0960-9822(09)00891-4, DOI: 10.1016/j.cub.2009.02.065, ISSN: 1879-0445.

Dinieri JA, Nemeth CL, Parsegian A, Carle T, Gurevich VV, Gurevich E, Neve RL, Nestler EJ, Carlezon WA. Altered sensitivity to rewarding and aversive drugs in mice with inducible disruption of cAMP response element-binding protein function within the nucleus accumbens. J. Neurosci. 2009 Feb 2/11/2009; 29(6): 1855-9. PMID: 19211892, PMCID: PMC2666984, PII: 29/6/1855, DOI: 10.1523/JNEUROSCI.5104-08.2009, ISSN: 1529-2401.

Wen XH, Shen L, Brush RS, Michaud N, Al-Ubaidi MR, Gurevich VV, Hamm HE, Lem J, Dibenedetto E, Anderson RE, Makino CL. Overexpression of rhodopsin alters the structure and photoresponse of rod photoreceptors. Biophys. J. 2009 Feb; 96(3): 939-50. PMID: 19186132, PMCID: PMC2716671, PII: S0006-3495(08)00054-4, DOI: 10.1016/j.bpj.2008.10.016, ISSN: 1542-0086.

Song X, Coffa S, Fu H, Gurevich VV. How does arrestin assemble MAPKs into a signaling complex?. J. Biol. Chem [print-electronic]. 2009 Jan 1/2/2009; 284(1): 685-95. PMID: 19001375, PMCID: PMC2610502, PII: M806124200, DOI: 10.1074/jbc.M806124200, ISSN: 0021-9258.

Lan H, Teeter MM, Gurevich VV, Neve KA. An intracellular loop 2 amino acid residue determines differential binding of arrestin to the dopamine D2 and D3 receptors. Mol. Pharmacol [print-electronic]. 2009 Jan; 75(1): 19-26. PMID: 18820126, PMCID: PMC2606909, PII: mol.108.050542, DOI: 10.1124/mol.108.050542, ISSN: 1521-0111.

Lan H, Liu Y, Bell MI, Gurevich VV, Neve KA. A dopamine D2 receptor mutant capable of G protein-mediated signaling but deficient in arrestin binding. Mol. Pharmacol [print-electronic]. 2009 Jan; 75(1): 113-23. PMID: 18809670, PMCID: PMC2606918, PII: mol.108.050534, DOI: 10.1124/mol.108.050534, ISSN: 1521-0111.

Wen XH, Shen L, Brush RS, Michaud N, Al-Ubaidi MR, Gurevich VV, Hamm HE, Lem J, Dibenedetto E, Anderson RE, Makino CL. Overexpression of rhodopsin alters the structure and photoresponse of rod photoreceptors. Biophys J. 2009; 96((3)): 93-50.

Gurevich VV, Gurevich EV. Rich tapestry of G protein-coupled receptor signaling and regulatory mechanisms. Mol. Pharmacol [print-electronic]. 2008 Aug; 74(2): 312-6. PMID: 18515421, PMCID: PMC2865845, PII: mol.108.049015, DOI: 10.1124/mol.108.049015, ISSN: 1521-0111.

Hanson SM, Dawson ES, Francis DJ, Van Eps N, Klug CS, Hubbell WL, Meiler J, Gurevich VV. A model for the solution structure of the rod arrestin tetramer. Structure. 2008 Jun; 16(6): 924-34. PMID: 18547524, PMCID: PMC2464289, PII: S0969-2126(08)00143-3, DOI: 10.1016/j.str.2008.03.006, ISSN: 0969-2126.

Gurevich VV, Gurevich EV. How and why do GPCRs dimerize?. Trends Pharmacol. Sci [print-electronic]. 2008 May; 29(5): 234-40. PMID: 18384890, PMCID: PMC2652501, PII: S0165-6147(08)00069-2, DOI: 10.1016/j.tips.2008.02.004, ISSN: 0165-6147.

Bisegna P, Caruso G, Andreucci D, Shen L, Gurevich VV, Hamm HE, DiBenedetto E. Diffusion of the second messengers in the cytoplasm acts as a variability suppressor of the single photon response in vertebrate phototransduction. Biophys. J. 2008 May 5/1/2008; 94(9): 3363-83. PMID: 18400950, PMCID: PMC2292384, PII: 94/9/3363, DOI: 10.1529/biophysj.107.114058, ISSN: 1542-0086.

Ahmed MR, Gurevich VV, Dalby KN, Benovic JL, Gurevich EV. Haloperidol and clozapine differentially affect the expression of arrestins, receptor kinases, and extracellular signal-regulated kinase activation. J. Pharmacol. Exp. Ther [print-electronic]. 2008 Apr; 325(1): 276-83. PMID: 18178904, PMCID: PMC2629350, PII: jpet.107.131987, DOI: 10.1124/jpet.107.131987, ISSN: 1521-0103.

Ahmed MR, Bychkov E, Gurevich VV, Benovic JL, Gurevich EV. Altered expression and subcellular distribution of GRK subtypes in the dopamine-depleted rat basal ganglia is not normalized by l-DOPA treatment. J. Neurochem [print-electronic]. 2008 Mar; 104(6): 1622-36. PMID: 17996024, PMCID: PMC2628845, PII: JNC5104, DOI: 10.1111/j.1471-4159.2007.05104.x, ISSN: 1471-4159.

Bychkov ER, Gurevich VV, Joyce JN, Benovic JL, Gurevich EV. Arrestins and two receptor kinases are upregulated in Parkinson's disease with dementia. Neurobiol. Aging [print-electronic]. 2008 Mar; 29(3): 379-96. PMID: 17125886, PMCID: PMC2275668, PII: S0197-4580(06)00389-7, DOI: 10.1016/j.neurobiolaging.2006.10.012, ISSN: 1558-1497.

Gurevich VV, Gurevich EV. GPCR monomers and oligomers: it takes all kinds. Trends Neurosci [print-electronic]. 2008 Feb; 31(2): 74-81. PMID: 18199492, PMCID: PMC2366802, PII: S0166-2236(07)00302-5, DOI: 10.1016/j.tins.2007.11.007, ISSN: 0166-2236.

Hanson SM, Vishnivetskiy SA, Hubbell WL, Gurevich VV. Opposing effects of inositol hexakisphosphate on rod arrestin and arrestin2 self-association. Biochemistry [print-electronic]. 2008 Jan 1/22/2008; 47(3): 1070-5. PMID: 18161994, PMCID: PMC2562240, DOI: 10.1021/bi7021359, ISSN: 0006-2960.

Gurevich VV, Gurevich EV, Cleghorn WM. Arrestins as multi-functional signaling adaptors. Handb Exp Pharmacol. 2008; (186): 15-37. PMID: 18491047, DOI: 10.1007/978-3-540-72843-6_2, ISSN: 0171-2004.

Vishnivetskiy SA, Raman D, Wei J, Kennedy MJ, Hurley JB, Gurevich VV. Regulation of arrestin binding by rhodopsin phosphorylation level. J. Biol. Chem [print-electronic]. 2007 Nov 11/2/2007; 282(44): 32075-83. PMID: 17848565, PMCID: PMC2638115, PII: M706057200, DOI: 10.1074/jbc.M706057200, ISSN: 0021-9258.

Song X, Gurevich EV, Gurevich VV. Cone arrestin binding to JNK3 and Mdm2: conformational preference and localization of interaction sites. J. Neurochem [print-electronic]. 2007 Nov; 103(3): 1053-62. PMID: 17680991, PMCID: PMC2430867, PII: JNC4842, DOI: 10.1111/j.1471-4159.2007.04842.x, ISSN: 0022-3042.

Chan S, Rubin WW, Mendez A, Liu X, Song X, Hanson SM, Craft CM, Gurevich VV, Burns ME, Chen J. Functional comparisons of visual arrestins in rod photoreceptors of transgenic mice. Invest. Ophthalmol. Vis. Sci. 2007 May; 48(5): 1968-75. PMID: 17460248, PMCID: PMC2933836, PII: 48/5/1968, DOI: 10.1167/iovs.06-1287, ISSN: 0146-0404.

Hanson SM, Cleghorn WM, Francis DJ, Vishnivetskiy SA, Raman D, Song X, Nair KS, Slepak VZ, Klug CS, Gurevich VV. Arrestin mobilizes signaling proteins to the cytoskeleton and redirects their activity. J. Mol. Biol [print-electronic]. 2007 Apr 4/27/2007; 368(2): 375-87. PMID: 17359998, PMCID: PMC1904837, PII: S0022-2836(07)00234-3, DOI: 10.1016/j.jmb.2007.02.053, ISSN: 0022-2836.

Hanson SM, Van Eps N, Francis DJ, Altenbach C, Vishnivetskiy SA, Arshavsky VY, Klug CS, Hubbell WL, Gurevich VV. Structure and function of the visual arrestin oligomer. EMBO J [print-electronic]. 2007 Mar 3/21/2007; 26(6): 1726-36. PMID: 17332750, PMCID: PMC1829381, PII: 7601614, DOI: 10.1038/sj.emboj.7601614, ISSN: 0261-4189.

Hanson SM, Gurevich EV, Vishnivetskiy SA, Ahmed MR, Song X, Gurevich VV. Each rhodopsin molecule binds its own arrestin. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2007 Feb 2/27/2007; 104(9): 3125-8. PMID: 17360618, PMCID: PMC1805568, PII: 0610886104, DOI: 10.1073/pnas.0610886104, ISSN: 0027-8424.

Wu N, Hanson SM, Francis DJ, Vishnivetskiy SA, Thibonnier M, Klug CS, Shoham M, Gurevich VV. Arrestin binding to calmodulin: a direct interaction between two ubiquitous signaling proteins. J. Mol. Biol [print-electronic]. 2006 Dec 12/15/2006; 364(5): 955-63. PMID: 17054984, PMCID: PMC1783800, PII: S0022-2836(06)01299-X, DOI: 10.1016/j.jmb.2006.09.075, ISSN: 0022-2836.

Song X, Raman D, Gurevich EV, Vishnivetskiy SA, Gurevich VV. Visual and both non-visual arrestins in their "inactive" conformation bind JNK3 and Mdm2 and relocalize them from the nucleus to the cytoplasm. J. Biol. Chem [print-electronic]. 2006 Jul 7/28/2006; 281(30): 21491-9. PMID: 16737965, PMCID: PMC2430869, PII: M603659200, DOI: 10.1074/jbc.M603659200, ISSN: 0021-9258.

Wu N, Macion-Dazard R, Nithianantham S, Xu Z, Hanson SM, Vishnivetskiy SA, Gurevich VV, Thibonnier M, Shoham M. Soluble mimics of the cytoplasmic face of the human V1-vascular vasopressin receptor bind arrestin2 and calmodulin. Mol. Pharmacol [print-electronic]. 2006 Jul; 70(1): 249-58. PMID: 16574744, PII: mol.105.018804, DOI: 10.1124/mol.105.018804, ISSN: 0026-895X.

Gurevich VV, Gurevich EV. The structural basis of arrestin-mediated regulation of G-protein-coupled receptors. Pharmacol. Ther [print-electronic]. 2006 Jun; 110(3): 465-502. PMID: 16460808, PMCID: PMC2562282, PII: S0163-7258(05)00211-1, DOI: 10.1016/j.pharmthera.2005.09.008, ISSN: 0163-7258.

Hanson SM, Francis DJ, Vishnivetskiy SA, Klug CS, Gurevich VV. Visual arrestin binding to microtubules involves a distinct conformational change. J. Biol. Chem [print-electronic]. 2006 Apr 4/7/2006; 281(14): 9765-72. PMID: 16461350, PMCID: PMC2430877, PII: M510738200, DOI: 10.1074/jbc.M510738200, ISSN: 0021-9258.

Hanson SM, Francis DJ, Vishnivetskiy SA, Kolobova EA, Hubbell WL, Klug CS, Gurevich VV. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2006 Mar 3/28/2006; 103(13): 4900-5. PMID: 16547131, PMCID: PMC1458767, PII: 0600733103, DOI: 10.1073/pnas.0600733103, ISSN: 0027-8424.

Hanson SM, Gurevich VV. The differential engagement of arrestin surface charges by the various functional forms of the receptor. J. Biol. Chem [print-electronic]. 2006 Feb 2/10/2006; 281(6): 3458-62. PMID: 16339758, PMCID: PMC2440687, PII: M512148200, DOI: 10.1074/jbc.M512148200, ISSN: 0021-9258.

Gurevich EV, Gurevich VV. Arrestins: ubiquitous regulators of cellular signaling pathways. Genome Biol. 2006; 7(9): 236. PMID: 17020596, PMCID: PMC1794542, PII: gb-2006-7-9-236, DOI: 10.1186/gb-2006-7-9-236, ISSN: 1474-760X.

Sutton RB, Vishnivetskiy SA, Robert J, Hanson SM, Raman D, Knox BE, Kono M, Navarro J, Gurevich VV. Crystal structure of cone arrestin at 2.3A: evolution of receptor specificity. J. Mol. Biol [print-electronic]. 2005 Dec 12/16/2005; 354(5): 1069-80. PMID: 16289201, PII: S0022-2836(05)01254-4, DOI: 10.1016/j.jmb.2005.10.023, ISSN: 0022-2836.

Carter JM, Gurevich VV, Prossnitz ER, Engen JR. Conformational differences between arrestin2 and pre-activated mutants as revealed by hydrogen exchange mass spectrometry. J. Mol. Biol. 2005 Aug 8/26/2005; 351(4): 865-78. PMID: 16045931, PII: S0022-2836(05)00720-5, DOI: 10.1016/j.jmb.2005.06.048, ISSN: 0022-2836.

Guigoni C, Aubert I, Li Q, Gurevich VV, Benovic JL, Ferry S, Mach U, Stark H, Leriche L, Håkansson K, Bioulac BH, Gross CE, Sokoloff P, Fisone G, Gurevich EV, Bloch B, Bezard E. Pathogenesis of levodopa-induced dyskinesia: focus on D1 and D3 dopamine receptors. Parkinsonism Relat. Disord. 2005 Jun; 11 Suppl 1: S25-9. PMID: 15885624, PII: S1353-8020(05)00038-6, DOI: 10.1016/j.parkreldis.2004.11.005, ISSN: 1353-8020.

Nair KS, Hanson SM, Mendez A, Gurevich EV, Kennedy MJ, Shestopalov VI, Vishnivetskiy SA, Chen J, Hurley JB, Gurevich VV, Slepak VZ. Light-dependent redistribution of arrestin in vertebrate rods is an energy-independent process governed by protein-protein interactions. Neuron. 2005 May 5/19/2005; 46(4): 555-67. PMID: 15944125, PMCID: PMC2752952, PII: S0896-6273(05)00279-5, DOI: 10.1016/j.neuron.2005.03.023, ISSN: 0896-6273.

Zhang R, Khoo MS, Wu Y, Yang Y, Grueter CE, Ni G, Price EE, Thiel W, Guatimosim S, Song LS, Madu EC, Shah AN, Vishnivetskaya TA, Atkinson JB, Gurevich VV, Salama G, Lederer WJ, Colbran RJ, Anderson ME. Calmodulin kinase II inhibition protects against structural heart disease. Nat. Med [print-electronic]. 2005 Apr; 11(4): 409-17. PMID: 15793582, PII: nm1215, DOI: 10.1038/nm1215, ISSN: 1078-8956.

Macey TA, Liu Y, Gurevich VV, Neve KA. Dopamine D1 receptor interaction with arrestin3 in neostriatal neurons. J. Neurochem. 2005 Apr; 93(1): 128-34. PMID: 15773912, PII: JNC2998, DOI: 10.1111/j.1471-4159.2004.02998.x, ISSN: 0022-3042.

Bezard E, Gross CE, Qin L, Gurevich VV, Benovic JL, Gurevich EV. L-DOPA reverses the MPTP-induced elevation of the arrestin2 and GRK6 expression and enhanced ERK activation in monkey brain. Neurobiol. Dis. 2005 Mar; 18(2): 323-35. PMID: 15686961, PII: S0969-9961(04)00240-2, DOI: 10.1016/j.nbd.2004.10.005, ISSN: 0969-9961.

Key TA, Vines CM, Wagener BM, Gurevich VV, Sklar LA, Prossnitz ER. Inhibition of chemoattractant N-formyl peptide receptor trafficking by active arrestins. Traffic. 2005 Feb; 6(2): 87-99. PMID: 15634210, PII: TRA248, DOI: 10.1111/j.1600-0854.2004.00248.x, ISSN: 1398-9219.

Gurevich EV, Benovic JL, Gurevich VV. Arrestin2 expression selectively increases during neural differentiation. J. Neurochem. 2004 Dec; 91(6): 1404-16. PMID: 15584917, PII: JNC2830, DOI: 10.1111/j.1471-4159.2004.02830.x, ISSN: 0022-3042.

Macey TA, Gurevich VV, Neve KA. Preferential Interaction between the dopamine D2 receptor and Arrestin2 in neostriatal neurons. Mol. Pharmacol [print-electronic]. 2004 Dec; 66(6): 1635-42. PMID: 15361545, PII: mol.104.001495, DOI: 10.1124/mol.104.001495, ISSN: 0026-895X.

Nair KS, Hanson SM, Kennedy MJ, Hurley JB, Gurevich VV, Slepak VZ. Direct binding of visual arrestin to microtubules determines the differential subcellular localization of its splice variants in rod photoreceptors. J. Biol. Chem [print-electronic]. 2004 Sep 9/24/2004; 279(39): 41240-8. PMID: 15272005, PII: M406768200, DOI: 10.1074/jbc.M406768200, ISSN: 0021-9258.

Gurevich VV, Gurevich EV. The molecular acrobatics of arrestin activation. Trends Pharmacol. Sci. 2004 Feb; 25(2): 105-11. PMID: 15102497, PII: S0165-6147(03)00387-0, DOI: 10.1016/j.tips.2003.12.008, ISSN: 0165-6147.

Vishnivetskiy SA, Hosey MM, Benovic JL, Gurevich VV. Mapping the arrestin-receptor interface. Structural elements responsible for receptor specificity of arrestin proteins. J. Biol. Chem [print-electronic]. 2004 Jan 1/9/2004; 279(2): 1262-8. PMID: 14530255, PII: M308834200, DOI: 10.1074/jbc.M308834200, ISSN: 0021-9258.

Gurevich VV, Gurevich EV. The new face of active receptor bound arrestin attracts new partners. Structure. 2003 Sep; 11(9): 1037-42. PMID: 12962621, PII: S0969212603001849, ISSN: 0969-2126.

Shi M, Bennett TA, Cimino DF, Maestas DC, Foutz TD, Gurevich VV, Sklar LA, Prossnitz ER. Functional capabilities of an N-formyl peptide receptor-G(alpha)(i)(2) fusion protein: assemblies with G proteins and arrestins. Biochemistry. 2003 Jun 6/24/2003; 42(24): 7283-93. PMID: 12809484, DOI: 10.1021/bi0341657, ISSN: 0006-2960.

Gray JA, Bhatnagar A, Gurevich VV, Roth BL. The interaction of a constitutively active arrestin with the arrestin-insensitive 5-HT(2A) receptor induces agonist-independent internalization. Mol. Pharmacol. 2003 May; 63(5): 961-72. PMID: 12695524, ISSN: 0026-895X.

Pan L, Gurevich EV, Gurevich VV. The nature of the arrestin x receptor complex determines the ultimate fate of the internalized receptor. J. Biol. Chem [print-electronic]. 2003 Mar 3/28/2003; 278(13): 11623-32. PMID: 12525498, PII: M209532200, DOI: 10.1074/jbc.M209532200, ISSN: 0021-9258.

Raman D, Osawa S, Gurevich VV, Weiss ER. The interaction with the cytoplasmic loops of rhodopsin plays a crucial role in arrestin activation and binding. J. Neurochem. 2003 Mar; 84(5): 1040-50. PMID: 12603828, PII: 1598, ISSN: 0022-3042.

Key TA, Foutz TD, Gurevich VV, Sklar LA, Prossnitz ER. N-formyl peptide receptor phosphorylation domains differentially regulate arrestin and agonist affinity. J. Biol. Chem [print-electronic]. 2003 Feb 2/7/2003; 278(6): 4041-7. PMID: 12424254, PII: M204687200, DOI: 10.1074/jbc.M204687200, ISSN: 0021-9258.

Vishnivetskiy SA, Hirsch JA, Velez MG, Gurevich YV, Gurevich VV. Transition of arrestin into the active receptor-binding state requires an extended interdomain hinge. J. Biol. Chem [print-electronic]. 2002 Nov 11/15/2002; 277(46): 43961-7. PMID: 12215448, PII: M206951200, DOI: 10.1074/jbc.M206951200, ISSN: 0021-9258.

DeGraff JL, Gurevich VV, Benovic JL. The third intracellular loop of alpha 2-adrenergic receptors determines subtype specificity of arrestin interaction. J. Biol. Chem [print-electronic]. 2002 Nov 11/8/2002; 277(45): 43247-52. PMID: 12205092, PII: M207495200, DOI: 10.1074/jbc.M207495200, ISSN: 0021-9258.

Hunzicker-Dunn M, Gurevich VV, Casanova JE, Mukherjee S. ARF6: a newly appreciated player in G protein-coupled receptor desensitization. FEBS Lett. 2002 Jun 6/19/2002; 521(1-3): 3-8. PMID: 12067715, PII: S0014579302028223, ISSN: 0014-5793.

Mukherjee S, Gurevich VV, Preninger A, Hamm HE, Bader MF, Fazleabas AT, Birnbaumer L, Hunzicker-Dunn M. Aspartic acid 564 in the third cytoplasmic loop of the luteinizing hormone/choriogonadotropin receptor is crucial for phosphorylation-independent interaction with arrestin2. J. Biol. Chem [print-electronic]. 2002 May 5/17/2002; 277(20): 17916-27. PMID: 11867621, PII: M110479200, DOI: 10.1074/jbc.M110479200, ISSN: 0021-9258.

Lowe JD, Celver JP, Gurevich VV, Chavkin C. Mu-Opioid receptors desensitize less rapidly than delta-opioid receptors due to less efficient activation of arrestin. J. Biol. Chem [print-electronic]. 2002 May 5/3/2002; 277(18): 15729-35. PMID: 11861651, PII: M200612200, DOI: 10.1074/jbc.M200612200, ISSN: 0021-9258.

Celver J, Vishnivetskiy SA, Chavkin C, Gurevich VV. Conservation of the phosphate-sensitive elements in the arrestin family of proteins. J. Biol. Chem [print-electronic]. 2002 Mar 3/15/2002; 277(11): 9043-8. PMID: 11782458, PII: M107400200, DOI: 10.1074/jbc.M107400200, ISSN: 0021-9258.

Potter RM, Key TA, Gurevich VV, Sklar LA, Prossnitz ER. Arrestin variants display differential binding characteristics for the phosphorylated N-formyl peptide receptor carboxyl terminus. J. Biol. Chem [print-electronic]. 2002 Mar 3/15/2002; 277(11): 8970-8. PMID: 11777932, PII: M111086200, DOI: 10.1074/jbc.M111086200, ISSN: 0021-9258.

Gurevich EV, Benovic JL, Gurevich VV. Arrestin2 and arrestin3 are differentially expressed in the rat brain during postnatal development. Neuroscience. 2002; 109(3): 421-36. PMID: 11823056, PII: S0306452201005115, ISSN: 0306-4522.

Bennett TA, Foutz TD, Gurevich VV, Sklar LA, Prossnitz ER. Partial phosphorylation of the N-formyl peptide receptor inhibits G protein association independent of arrestin binding. J. Biol. Chem [print-electronic]. 2001 Dec 12/28/2001; 276(52): 49195-203. PMID: 11602585, PII: M106414200, DOI: 10.1074/jbc.M106414200, ISSN: 0021-9258.

Key TA, Bennett TA, Foutz TD, Gurevich VV, Sklar LA, Prossnitz ER. Regulation of formyl peptide receptor agonist affinity by reconstitution with arrestins and heterotrimeric G proteins. J. Biol. Chem [print-electronic]. 2001 Dec 12/28/2001; 276(52): 49204-12. PMID: 11598142, PII: M109475200, DOI: 10.1074/jbc.M109475200, ISSN: 0021-9258.

Han M, Gurevich VV, Vishnivetskiy SA, Sigler PB, Schubert C. Crystal structure of beta-arrestin at 1.9 A: possible mechanism of receptor binding and membrane Translocation. Structure. 2001 Sep; 9(9): 869-80. PMID: 11566136, PII: S096921260100644X, ISSN: 0969-2126.

Bennett TA, Key TA, Gurevich VV, Neubig R, Prossnitz ER, Sklar LA. Real-time analysis of G protein-coupled receptor reconstitution in a solubilized system. J. Biol. Chem [print-electronic]. 2001 Jun 6/22/2001; 276(25): 22453-60. PMID: 11309376, PII: M009679200, DOI: 10.1074/jbc.M009679200, ISSN: 0021-9258.

Celver JP, Lowe J, Kovoor A, Gurevich VV, Chavkin C. Threonine 180 is required for G-protein-coupled receptor kinase 3- and beta-arrestin 2-mediated desensitization of the mu-opioid receptor in Xenopus oocytes. J. Biol. Chem [print-electronic]. 2001 Feb 2/16/2001; 276(7): 4894-900. PMID: 11060299, PII: M007437200, DOI: 10.1074/jbc.M007437200, ISSN: 0021-9258.

Vishnivetskiy SA, Schubert C, Climaco GC, Gurevich YV, Velez MG, Gurevich VV. An additional phosphate-binding element in arrestin molecule. Implications for the mechanism of arrestin activation. J. Biol. Chem. 2000 Dec 12/29/2000; 275(52): 41049-57. PMID: 11024026, PII: M007159200, DOI: 10.1074/jbc.M007159200, ISSN: 0021-9258.

Smith WC, Gurevich EV, Dugger DR, Vishnivetskiy SA, Shelamer CL, McDowell JH, Gurevich VV. Cloning and functional characterization of salamander rod and cone arrestins. Invest. Ophthalmol. Vis. Sci. 2000 Aug; 41(9): 2445-55. PMID: 10937552, ISSN: 0146-0404.

Mushegian AR, Vishnivetskiy SA, Gurevich VV. Conserved phosphoprotein interaction motif is functionally interchangeable between ataxin-7 and arrestins. Biochemistry. 2000 Jun 6/13/2000; 39(23): 6809-13. PMID: 10841760, PII: bi992694y, ISSN: 0006-2960.

Mukherjee S, Gurevich VV, Jones JC, Casanova JE, Frank SR, Maizels ET, Bader MF, Kahn RA, Palczewski K, Aktories K, Hunzicker-Dunn M. The ADP ribosylation factor nucleotide exchange factor ARNO promotes beta-arrestin release necessary for luteinizing hormone/choriogonadotropin receptor desensitization. Proc. Natl. Acad. Sci. U.S.A. 2000 May 5/23/2000; 97(11): 5901-6. PMID: 10811902, PMCID: PMC18531, PII: 100127097, DOI: 10.1073/pnas.100127097, ISSN: 0027-8424.

Lee KB, Ptasienski JA, Pals-Rylaarsdam R, Gurevich VV, Hosey MM. Arrestin binding to the M(2) muscarinic acetylcholine receptor is precluded by an inhibitory element in the third intracellular loop of the receptor. J. Biol. Chem. 2000 Mar 3/31/2000; 275(13): 9284-9. PMID: 10734068, ISSN: 0021-9258.

Gurevich VV, Benovic JL. Arrestin: mutagenesis, expression, purification, and functional characterization. Meth. Enzymol. 2000; 315: 422-37. PMID: 10736718, ISSN: 0076-6879.

Schubert C, Hirsch JA, Gurevich VV, Engelman DM, Sigler PB, Fleming KG. Visual arrestin activity may be regulated by self-association. J. Biol. Chem. 1999 Jul 7/23/1999; 274(30): 21186-90. PMID: 10409673, ISSN: 0021-9258.

Mukherjee S, Palczewski K, Gurevich VV, Hunzicker-Dunn M. Beta-arrestin-dependent desensitization of luteinizing hormone/choriogonadotropin receptor is prevented by a synthetic peptide corresponding to the third intracellular loop of the receptor. J. Biol. Chem. 1999 May 5/7/1999; 274(19): 12984-9. PMID: 10224047, ISSN: 0021-9258.

Vishnivetskiy SA, Paz CL, Schubert C, Hirsch JA, Sigler PB, Gurevich VV. How does arrestin respond to the phosphorylated state of rhodopsin?. J. Biol. Chem. 1999 Apr 4/23/1999; 274(17): 11451-4. PMID: 10206946, ISSN: 0021-9258.

Hirsch JA, Schubert C, Gurevich VV, Sigler PB. The 2.8 A crystal structure of visual arrestin: a model for arrestin's regulation. Cell. 1999 Apr 4/16/1999; 97(2): 257-69. PMID: 10219246, PII: S0092-8674(00)80735-7, ISSN: 0092-8674.

Kovoor A, Celver J, Abdryashitov RI, Chavkin C, Gurevich VV. Targeted construction of phosphorylation-independent beta-arrestin mutants with constitutive activity in cells. J. Biol. Chem. 1999 Mar 3/12/1999; 274(11): 6831-4. PMID: 10066734, ISSN: 0021-9258.

Hosey MM, Pals-Rylaarsdam R, Lee KB, Roseberry AG, Benovic JL, Gurevich VV, Bünemann M. Molecular events associated with the regulation of signaling by M2 muscarinic receptors. Life Sci. 1999; 64(6-7): 363-8. PMID: 10069497, ISSN: 0024-3205.

Gurevich VV. The selectivity of visual arrestin for light-activated phosphorhodopsin is controlled by multiple nonredundant mechanisms. J. Biol. Chem. 1998 Jun 6/19/1998; 273(25): 15501-6. PMID: 9624137, ISSN: 0021-9258.

Goodman OB, Krupnick JG, Santini F, Gurevich VV, Penn RB, Gagnon AW, Keen JH, Benovic JL. Role of arrestins in G-protein-coupled receptor endocytosis. Adv. Pharmacol. 1998; 42: 429-33. PMID: 9327931, ISSN: 1054-3589.

Gurevich VV, Pals-Rylaarsdam R, Benovic JL, Hosey MM, Onorato JJ. Agonist-receptor-arrestin, an alternative ternary complex with high agonist affinity. J. Biol. Chem. 1997 Nov 11/14/1997; 272(46): 28849-52. PMID: 9360951, ISSN: 0021-9258.

Pals-Rylaarsdam R, Gurevich VV, Lee KB, Ptasienski JA, Benovic JL, Hosey MM. Internalization of the m2 muscarinic acetylcholine receptor. Arrestin-independent and -dependent pathways. J. Biol. Chem. 1997 Sep 9/19/1997; 272(38): 23682-9. PMID: 9295310, ISSN: 0021-9258.

Krupnick JG, Gurevich VV, Benovic JL. Mechanism of quenching of phototransduction. Binding competition between arrestin and transducin for phosphorhodopsin. J. Biol. Chem. 1997 Jul 7/18/1997; 272(29): 18125-31. PMID: 9218446, ISSN: 0021-9258.

Gray-Keller MP, Detwiler PB, Benovic JL, Gurevich VV. Arrestin with a single amino acid substitution quenches light-activated rhodopsin in a phosphorylation-independent fashion. Biochemistry. 1997 Jun 6/10/1997; 36(23): 7058-63. PMID: 9188704, PII: bi963110k, DOI: 10.1021/bi963110k, ISSN: 0006-2960.

Goodman OB, Krupnick JG, Gurevich VV, Benovic JL, Keen JH. Arrestin/clathrin interaction. Localization of the arrestin binding locus to the clathrin terminal domain. J. Biol. Chem. 1997 Jun 6/6/1997; 272(23): 15017-22. PMID: 9169477, ISSN: 0021-9258.

Gurevich VV, Benovic JL. Mechanism of phosphorylation-recognition by visual arrestin and the transition of arrestin into a high affinity binding state. Mol. Pharmacol. 1997 Jan; 51(1): 161-9. PMID: 9016359, ISSN: 0026-895X.

Goodman OB, Krupnick JG, Santini F, Gurevich VV, Penn RB, Gagnon AW, Keen JH, Benovic JL. Beta-arrestin acts as a clathrin adaptor in endocytosis of the beta2-adrenergic receptor. Nature. 1996 Oct 10/3/1996; 383(6599): 447-50. PMID: 8837779, DOI: 10.1038/383447a0, ISSN: 0028-0836.

Gurevich VV. Use of bacteriophage RNA polymerase in RNA synthesis. Meth. Enzymol. 1996; 275: 382-97. PMID: 9026651, ISSN: 0076-6879.

Gurevich VV, Benovic JL. Visual arrestin binding to rhodopsin. Diverse functional roles of positively charged residues within the phosphorylation-recognition region of arrestin. J. Biol. Chem. 1995 Mar 3/17/1995; 270(11): 6010-6. PMID: 7890732, ISSN: 0021-9258.

Gurevich VV, Dion SB, Onorato JJ, Ptasienski J, Kim CM, Sterne-Marr R, Hosey MM, Benovic JL. Arrestin interactions with G protein-coupled receptors. Direct binding studies of wild type and mutant arrestins with rhodopsin, beta 2-adrenergic, and m2 muscarinic cholinergic receptors. J. Biol. Chem. 1995 Jan 1/13/1995; 270(2): 720-31. PMID: 7822302, ISSN: 0021-9258.

Pokrovskaya ID, Gurevich VV. In vitro transcription: preparative RNA yields in analytical scale reactions. Anal. Biochem. 1994 Aug 8/1/1994; 220(2): 420-3. PMID: 7526740, PII: S0003-2697(84)71360-1, DOI: 10.1006/abio.1994.1360, ISSN: 0003-2697.

Kunapuli P, Gurevich VV, Benovic JL. Phospholipid-stimulated autophosphorylation activates the G protein-coupled receptor kinase GRK5. J. Biol. Chem. 1994 Apr 4/8/1994; 269(14): 10209-12. PMID: 8144599, ISSN: 0021-9258.

Gurevich VV, Chen CY, Kim CM, Benovic JL. Visual arrestin binding to rhodopsin. Intramolecular interaction between the basic N terminus and acidic C terminus of arrestin may regulate binding selectivity. J. Biol. Chem. 1994 Mar 3/25/1994; 269(12): 8721-7. PMID: 8132602, ISSN: 0021-9258.

Krupnick JG, Gurevich VV, Schepers T, Hamm HE, Benovic JL. Arrestin-rhodopsin interaction. Multi-site binding delineated by peptide inhibition. J. Biol. Chem. 1994 Feb 2/4/1994; 269(5): 3226-32. PMID: 8106358, ISSN: 0021-9258.

Gurevich VV, Richardson RM, Kim CM, Hosey MM, Benovic JL. Binding of wild type and chimeric arrestins to the m2 muscarinic cholinergic receptor. J. Biol. Chem. 1993 Aug 8/15/1993; 268(23): 16879-82. PMID: 8349577, ISSN: 0021-9258.

Sterne-Marr R, Gurevich VV, Goldsmith P, Bodine RC, Sanders C, Donoso LA, Benovic JL. Polypeptide variants of beta-arrestin and arrestin3. J. Biol. Chem. 1993 Jul 7/25/1993; 268(21): 15640-8. PMID: 8340388, ISSN: 0021-9258.

Gurevich VV, Benovic JL. Visual arrestin interaction with rhodopsin. Sequential multisite binding ensures strict selectivity toward light-activated phosphorylated rhodopsin. J. Biol. Chem. 1993 Jun 6/5/1993; 268(16): 11628-38. PMID: 8505295, ISSN: 0021-9258.

Gurevich VV, Benovic JL. Cell-free expression of visual arrestin. Truncation mutagenesis identifies multiple domains involved in rhodopsin interaction. J. Biol. Chem. 1992 Oct 10/25/1992; 267(30): 21919-23. PMID: 1400502, ISSN: 0021-9258.

Gurevich VV, Pokrovskaya ID, Obukhova TA, Zozulya SA. Preparative in vitro mRNA synthesis using SP6 and T7 RNA polymerases. Anal. Biochem. 1991 Jun; 195(2): 207-13. PMID: 1750668, ISSN: 0003-2697.

Gurevich VV, Pokrovskaya ID, Garnovskaya MN, Dumler IL, Zozulya SA. Proper cotranslational insertion of visual rhodopsin into the lipid bilayer occurs in the absence of protein translocation machinery. Biomed. Sci. 1991; 2(2): 187-92. PMID: 1663398, ISSN: 0955-9701.

Zozulya SA, Gurevich VV, Zvyaga TA, Shirokova EP, Dumler IL, Garnovskaya MN, Natochin MYu, Shmukler BE, Badalov PR. Functional expression in vitro of bovine visual rhodopsin. Protein Eng. 1990 Apr; 3(5): 453-8. PMID: 2140896, ISSN: 0269-2139.

Gurevich VV, Zozulia SA, Shirokova EP, Zviaga TA, Garnovskaia MN, Dumler IL, Badalov PR, Natochin MIu, Pokrovskaia ID, Shmukler BE. [Synthesis of visual rhodopsin in a cell-free translation system. II. Functional properties of recombinant rhodopsin and its mutant forms]. Bioorg. Khim. 1990 Mar; 16(3): 303-8. PMID: 2357235, ISSN: 0132-3423.

Gurevich VV, Zozulya SA, Zerf EP, Pokrovskaya ID, Obukhova TA, Garnovskaya MN, Dumler IL, Rychkova MP. Bovine rhodopsin: amino acid substitutions Asp-83----Asn and Glu-134----Gln prevent activation of cyclic GMP phosphodiesterase. Biomed. Sci. 1990; 1(5): 527-30. PMID: 1966786, ISSN: 0955-9701.

Zozulya SA, Gurevich VV, Zvyaga TA, Dumler IL, Garnovskaya MN, Shmukler BE, Natochin MYu, Shirokova EP, Badalov PR. In vitro synthesis of visual rhodopsin for a protein engineering study. J. Protein Chem. 1989 Jun; 8(3): 380-2. PMID: 2528964, ISSN: 0277-8033.

Zozulia SA, Gurevich VV, Shmukler BE, Namochin MIu, Zviaga TA. [Synthesis of visual rhodopsin in a cell-free translation system. I. Influence of the structure of the synthetic bovine visual opsin mRNA on its translational efficiency]. Bioorg. Khim. 1988 Dec; 14(12): 1663-70. PMID: 2978006, ISSN: 0132-3423.

Voeikov VL, Gurevich VV. [Separation of [3H]dihydroalprenolol, free or bound to solubilized beta-adrenergic receptors, on a cation-exchange resin]. Bioorg. Khim. 1983 May; 9(5): 628-32. PMID: 6091694, ISSN: 0132-3423.

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