Jamey D. Young, Ph.D.

Associate Professor


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Faculty Appointments
Director of Graduate Recruiting for Chemical and Biomolecular Engineering Associate Professor of Chemical and Biomolecular Engineering Chancellor Faculty Fellow
Ph.D., Chemical Engineering, Purdue University, West Lafayette, IndianaB.S., Chemical Engineering, University of Kentucky, Lexington, Kentucky
Office Address
VU Station B #351604
2400 Highland Ave; 107 Olin Hall
2301 Vanderbilt Place
Nashville, TN 37235-1304
Research Description
The central theme of our research is the application of engineering, biochemistry, and molecular biology to quantitatively analyze and redirect cellular metabolism. In particular, we use an approach called metabolic flux analysis (MFA) to quantify the rates, or "fluxes", of intracellular metabolic pathways in a variety of cell models of relevance to medicine and biotechnology. In addition to tool-oriented research aimed at Enhancing MFA Methodologies, we are actively pursuing research in four specific application areas: Diabetes & Obesity, Cancer Metabolism, Cell Culture Engineering, and Photosynthesis Research. Although diverse in their biological scope, all projects rely on our lab's core expertise to integrate numerous metabolic measurements into a quantitative map of cell metabolism using rigorous mathematical models, with the ultimate goal of deciphering how metabolic networks are natively regulated and how they can be further engineered to achieve a desired outcome.
Research Keywords
Metabolic engineering and systems biology
Rahman SM, Ji X, Zimmerman LJ, Li M, Harris BK, Hoeksema MD, Trenary IA, Zou Y, Qian J, Slebos RJ, Beane J, Spira A, Shyr Y, Eisenberg R, Liebler DC, Young JD, Massion PP. The airway epithelium undergoes metabolic reprogramming in individuals at high risk for lung cancer. JCI Insight. 2016 Nov 11/17/2016; 1(19): e88814. PMID: 27882349, PMCID: PMC5111517, PII: 88814, DOI: 10.1172/jci.insight.88814.

Leamy AK, Hasenour CM, Egnatchik RA, Trenary IA, Yao CH, Patti GJ, Shiota M, Young JD. Knockdown of triglyceride synthesis does not enhance palmitate lipotoxicity or prevent oleate-mediated rescue in rat hepatocytes. Biochim. Biophys. Acta [print-electronic]. 2016 Sep; 1861(9 Pt A): 1005-14. PMID: 27249207, PMCID: PMC4947436, PII: S1388-1981(16)30142-1, DOI: 10.1016/j.bbalip.2016.05.013, ISSN: 0006-3002.

Hutton JE, Wang X, Zimmerman LJ, Slebos RJ, Trenary IA, Young JD, Li M, Liebler DC. Oncogenic KRAS and BRAF drive metabolic reprogramming in colorectal cancer. Mol. Cell Proteomics [print-electronic]. 2016 Jun 6/23/2016; PMID: 27340238, PII: M116.058925, DOI: 10.1074/mcp.M116.058925, ISSN: 1535-9484.

McCloskey D, Young JD, Xu S, Palsson BO, Feist AM. Modeling Method for Increased Precision and Scope of Directly Measurable Fluxes at a Genome-Scale. Anal. Chem [print-electronic]. 2016 Apr 4/5/2016; 88(7): 3844-52. PMID: 26981784, DOI: 10.1021/acs.analchem.5b04914, ISSN: 1520-6882.

Whang YM, Park SI, Trenary IA, Egnatchik RA, Fessel JP, Kaufman JM, Carbone DP, Young JD. LKB1 deficiency enhances sensitivity to energetic stress induced by erlotinib treatment in non-small-cell lung cancer (NSCLC) cells. Oncogene [print-electronic]. 2016 Feb 2/18/2016; 35(7): 856-66. PMID: 26119936, PMCID: PMC4486321, PII: onc2015140, DOI: 10.1038/onc.2015.140, ISSN: 1476-5594.

McCloskey D, Young JD, Xu S, Palsson BO, Feist AM. MID Max: LC-MS/MS Method for Measuring the Precursor and Product Mass Isotopomer Distributions of Metabolic Intermediates and Cofactors for Metabolic Flux Analysis Applications. Anal. Chem [print-electronic]. 2016 Jan 1/19/2016; 88(2): 1362-70. PMID: 26666286, DOI: 10.1021/acs.analchem.5b03887, ISSN: 1520-6882.

McAtee AG, Jazmin LJ, Young JD. Application of isotope labeling experiments and (13)C flux analysis to enable rational pathway engineering. Curr. Opin. Biotechnol [print-electronic]. 2015 Dec; 36: 50-6. PMID: 26319894, PII: S0958-1669(15)00103-2, DOI: 10.1016/j.copbio.2015.08.004, ISSN: 1879-0429.

Adebiyi AO, Jazmin LJ, Young JD. 13 C flux analysis of cyanobacterial metabolism. Photosyn. Res [print-electronic]. 2015 Oct; 126(1): 19-32. PMID: 25280933, PII: 10.1007/s11120-014-0045-1, DOI: 10.1007/s11120-014-0045-1, ISSN: 1573-5079.

Buescher JM, Antoniewicz MR, Boros LG, Burgess SC, Brunengraber H, Clish CB, DeBerardinis RJ, Feron O, Frezza C, Ghesquiere B, Gottlieb E, Hiller K, Jones RG, Kamphorst JJ, Kibbey RG, Kimmelman AC, Locasale JW, Lunt SY, Maddocks OD, Malloy C, Metallo CM, Meuillet EJ, Munger J, Nöh K, Rabinowitz JD, Ralser M, Sauer U, Stephanopoulos G, St-Pierre J, Tennant DA, Wittmann C, Vander Heiden MG, Vazquez A, Vousden K, Young JD, Zamboni N, Fendt SM. A roadmap for interpreting (13)C metabolite labeling patterns from cells. Curr. Opin. Biotechnol [print-electronic]. 2015 Aug; 34: 189-201. PMID: 25731751, PMCID: PMC4552607, PII: S0958-1669(15)00022-1, DOI: 10.1016/j.copbio.2015.02.003, ISSN: 1879-0429.

Hasenour CM, Wall ML, Ridley DE, Hughey CC, James FD, Wasserman DH, Young JD. Mass spectrometry-based microassay of (2)H and (13)C plasma glucose labeling to quantify liver metabolic fluxes in vivo. Am. J. Physiol. Endocrinol. Metab [print-electronic]. 2015 Jul 7/15/2015; 309(2): E191-203. PMID: 25991647, PMCID: PMC4504936, PII: ajpendo.00003.2015, DOI: 10.1152/ajpendo.00003.2015, ISSN: 1522-1555.

Wall ML, Pound LD, Trenary I, O'Brien RM, Young JD. Novel stable isotope analyses demonstrate significant rates of glucose cycling in mouse pancreatic islets. Diabetes [print-electronic]. 2015 Jun; 64(6): 2129-37. PMID: 25552595, PMCID: PMC4439557, PII: db14-0745, DOI: 10.2337/db14-0745, ISSN: 1939-327X.

Young JD. (13)C metabolic flux analysis of recombinant expression hosts. Curr. Opin. Biotechnol [print-electronic]. 2014 Dec; 30: 238-45. PMID: 25456032, PII: S0958-1669(14)00172-4, DOI: 10.1016/j.copbio.2014.10.004, ISSN: 1879-0429.

Junker BH, Young JD. Editorial overview: pharmaceutical biotechnology: engineering cells for high quality biopharmaceuticals production [editorial]. Curr. Opin. Biotechnol. 2014 Dec; 30: viii-x. PMID: 25459164, PII: S0958-1669(14)00170-0, DOI: 10.1016/j.copbio.2014.10.002, ISSN: 1879-0429.

Ma F, Jazmin LJ, Young JD, Allen DK. Isotopically nonstationary 13C flux analysis of changes in Arabidopsis thaliana leaf metabolism due to high light acclimation. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2014 Nov 11/25/2014; 111(47): 16967-72. PMID: 25368168, PMCID: PMC4250135, PII: 1319485111, DOI: 10.1073/pnas.1319485111, ISSN: 1091-6490.

Templeton N, Lewis A, Dorai H, Qian EA, Campbell MP, Smith KD, Lang SE, Betenbaugh MJ, Young JD. The impact of anti-apoptotic gene Bcl-2¿ expression on CHO central metabolism. Metab. Eng [print-electronic]. 2014 Sep; 25: 92-102. PMID: 25014175, PII: S1096-7176(14)00091-3, DOI: 10.1016/j.ymben.2014.06.010, ISSN: 1096-7184.

Egnatchik RA, Leamy AK, Jacobson DA, Shiota M, Young JD. ER calcium release promotes mitochondrial dysfunction and hepatic cell lipotoxicity in response to palmitate overload. Mol Metab. 2014 Aug; 3(5): 544-53. PMID: 25061559, PMCID: PMC4099508, PII: S2212-8778(14)00092-1, DOI: 10.1016/j.molmet.2014.05.004, ISSN: 2212-8778.

Leamy AK, Egnatchik RA, Shiota M, Ivanova PT, Myers DS, Brown HA, Young JD. Enhanced synthesis of saturated phospholipids is associated with ER stress and lipotoxicity in palmitate treated hepatic cells. J. Lipid Res [print-electronic]. 2014 May 5/23/2014; 55(7): 1478-88. PMID: 24859739, PMCID: PMC4076085, PII: jlr.M050237, DOI: 10.1194/jlr.M050237, ISSN: 0022-2275.

Young JD. INCA: a computational platform for isotopically non-stationary metabolic flux analysis. Bioinformatics [print-electronic]. 2014 May 5/1/2014; 30(9): 1333-5. PMID: 24413674, PMCID: PMC3998137, PII: btu015, DOI: 10.1093/bioinformatics/btu015, ISSN: 1367-4811.

Egnatchik RA, Leamy AK, Noguchi Y, Shiota M, Young JD. Palmitate-induced activation of mitochondrial metabolism promotes oxidative stress and apoptosis in H4IIEC3 rat hepatocytes. Metab. Clin. Exp [print-electronic]. 2014 Feb; 63(2): 283-95. PMID: 24286856, PMCID: PMC3946971, PII: S0026-0495(13)00341-7, DOI: 10.1016/j.metabol.2013.10.009, ISSN: 1532-8600.

Jazmin LJ, O'Grady JP, Ma F, Allen DK, Morgan JA, Young JD. Isotopically nonstationary MFA (INST-MFA) of autotrophic metabolism. Methods Mol. Biol. 2014; 1090: 181-210. PMID: 24222417, DOI: 10.1007/978-1-62703-688-7_12, ISSN: 1940-6029.

Young JD, Allen DK, Morgan JA. Isotopomer measurement techniques in metabolic flux analysis II: mass spectrometry. Methods Mol. Biol. 2014; 1083: 85-108. PMID: 24218212, DOI: 10.1007/978-1-62703-661-0_7, ISSN: 1940-6029.

Young JD. Metabolic flux rewiring in mammalian cell cultures. Curr. Opin. Biotechnol [print-electronic]. 2013 Dec; 24(6): 1108-15. PMID: 23726154, PMCID: PMC3775942, PII: S0958-1669(13)00114-6, DOI: 10.1016/j.copbio.2013.04.016, ISSN: 1879-0429.

Templeton N, Dean J, Reddy P, Young JD. Peak antibody production is associated with increased oxidative metabolism in an industrially relevant fed-batch CHO cell culture. Biotechnol. Bioeng [print-electronic]. 2013 Jul; 110(7): 2013-24. PMID: 23381838, DOI: 10.1002/bit.24858, ISSN: 1097-0290.

Murphy TA, Young JD. ETA: robust software for determination of cell specific rates from extracellular time courses. Biotechnol. Bioeng [print-electronic]. 2013 Jun; 110(6): 1748-58. PMID: 23296385, PMCID: PMC3863648, DOI: 10.1002/bit.24836, ISSN: 1097-0290.

Allen DK, Young JD. Carbon and nitrogen provisions alter the metabolic flux in developing soybean embryos. Plant Physiol [print-electronic]. 2013 Mar; 161(3): 1458-75. PMID: 23314943, PMCID: PMC3585609, PII: pp.112.203299, DOI: 10.1104/pp.112.203299, ISSN: 1532-2548.

Murphy TA, Dang CV, Young JD. Isotopically nonstationary 13C flux analysis of Myc-induced metabolic reprogramming in B-cells. Metab. Eng [print-electronic]. 2013 Jan; 15: 206-17. PMID: 22898717, PMCID: PMC3535509, PII: S1096-7176(12)00078-X, DOI: 10.1016/j.ymben.2012.07.008, ISSN: 1096-7184.

Duckwall CS, Murphy TA, Young JD. Mapping cancer cell metabolism with(13)C flux analysis: Recent progress and future challenges. J Carcinog. 2013; 12: 13. PMID: 23961260, PMCID: PMC3746411, PII: JC-12-13, DOI: 10.4103/1477-3163.115422, ISSN: 1477-3163.

Jazmin LJ, Young JD. Isotopically nonstationary 13C metabolic flux analysis. Methods Mol. Biol. 2013; 985: 367-90. PMID: 23417813, DOI: 10.1007/978-1-62703-299-5_18, ISSN: 1940-6029.

Leamy AK, Egnatchik RA, Young JD. Molecular mechanisms and the role of saturated fatty acids in the progression of non-alcoholic fatty liver disease. Prog. Lipid Res [print-electronic]. 2013 Jan; 52(1): 165-74. PMID: 23178552, PMCID: PMC3868987, PII: S0163-7827(12)00058-6, DOI: 10.1016/j.plipres.2012.10.004, ISSN: 1873-2194.

Strand DW, Jiang M, Murphy TA, Yi Y, Konvinse KC, Franco OE, Wang Y, Young JD, Hayward SW. PPAR-gamma isoforms differentially regulate metabolic networks to mediate mouse prostatic epithelial differentiation. Cell Death Dis. 2012; 3: e361. PMID: 22874998, PMCID: PMC3434663, PII: cddis201299, DOI: 10.1038/cddis.2012.99, ISSN: 2041-4889.

Young JD, Shastri AA, Stephanopoulos G, Morgan JA. Mapping photoautotrophic metabolism with isotopically nonstationary (13)C flux analysis. Metab. Eng [print-electronic]. 2011 Nov; 13(6): 656-65. PMID: 21907300, PMCID: PMC3210925, PII: S1096-7176(11)00088-7, DOI: 10.1016/j.ymben.2011.08.002, ISSN: 1096-7184.

Noguchi Y, Young JD, Aleman JO, Hansen ME, Kelleher JK, Stephanopoulos G. Tracking cellular metabolomics in lipoapoptosis- and steatosis-developing liver cells. Mol Biosyst [print-electronic]. 2011 May; 7(5): 1409-19. PMID: 21327189, DOI: 10.1039/c0mb00309c, ISSN: 1742-2051.

Srour O, Young JD, Eldar YC. Fluxomers: a new approach for 13C metabolic flux analysis. BMC Syst Biol. 2011; 5: 129. PMID: 21846358, PMCID: PMC3750106, PII: 1752-0509-5-129, DOI: 10.1186/1752-0509-5-129, ISSN: 1752-0509.

Noguchi Y, Nishikata N, Shikata N, Kimura Y, Aleman JO, Young JD, Koyama N, Kelleher JK, Takahashi M, Stephanopoulos G. Ketogenic essential amino acids modulate lipid synthetic pathways and prevent hepatic steatosis in mice. PLoS ONE. 2010; 5(8): e12057. PMID: 20706589, PMCID: PMC2919399, DOI: 10.1371/journal.pone.0012057, ISSN: 1932-6203.

Reed JL, Senger RS, Antoniewicz MR, Young JD. Computational approaches in metabolic engineering [editorial]. J. Biomed. Biotechnol [print-electronic]. 2010; 2010: 207414. PMID: 21584279, PMCID: PMC3092504, DOI: 10.1155/2010/207414, ISSN: 1110-7251.

Noguchi Y, Young JD, Aleman JO, Hansen ME, Kelleher JK, Stephanopoulos G. Effect of anaplerotic fluxes and amino acid availability on hepatic lipoapoptosis. J. Biol. Chem [print-electronic]. 2009 Nov 11/27/2009; 284(48): 33425-36. PMID: 19758988, PMCID: PMC2785187, PII: M109.049478, DOI: 10.1074/jbc.M109.049478, ISSN: 1083-351X.

Young JD, Henne KL, Morgan JA, Konopka AE, Ramkrishna D. Integrating cybernetic modeling with pathway analysis provides a dynamic, systems-level description of metabolic control. Biotechnol. Bioeng. 2008 Jun 6/15/2008; 100(3): 542-59. PMID: 18438875, DOI: 10.1002/bit.21780, ISSN: 1097-0290.

Young JD, Walther JL, Antoniewicz MR, Yoo H, Stephanopoulos G. An elementary metabolite unit (EMU) based method of isotopically nonstationary flux analysis. Biotechnol. Bioeng. 2008 Feb 2/15/2008; 99(3): 686-99. PMID: 17787013, DOI: 10.1002/bit.21632, ISSN: 1097-0290.

Young JD, Ramkrishna D. On the matching and proportional laws of cybernetic models. Biotechnol. Prog. 2007 Jan; 23(1): 83-99. PMID: 17269675, DOI: 10.1021/bp060176q, ISSN: 1520-6033.

J. D. Young, K. L. Henne, J. A. Morgan, A. E. Konopka and D. Ramkrishna.. Cybernetic modeling of metabolism: towards a framework for rational design of recombinant organisms. Chemical Engineering Science. 2004; 59: 5041-9.

Available Postdoctoral Position Details
Posted: 12/1/2016

Postdoctoral Position in Diabetes and Obesity Research
Jamey Young’s Lab at Vanderbilt University

A postdoctoral position is available immediately to study the pathogenesis of fatty liver disease using in vivo mouse models and novel isotope tracer technologies. The successful candidate will apply adenoviral gene delivery to test the effects of specific proteins in altering disease progression in mice. 2H/13C flux analysis will be applied to assess the effects of transgene expression on hepatic metabolism. Motivation and experience will be important factors in the recruitment process. For more information about the Young laboratory, see our website: http://www.vanderbilt.edu/younglab. Interested applicants should send a current CV including the names of three references to: Dr. Jamey Young at j.d.young@vanderbilt.edu.