H. Alex Brown, Ph.D.



Visit Lab Site

Faculty Appointments
Professor of Pharmacology Professor of BiochemistryBixler-Johnson-Mayes Chair
Ph.D., Neurobiology/Pharmacology, University of North Carolina, Chapel Hill, North CarolinaM.S., Neuroscience, Syracuse University, Syracuse, New YorkB.S., Florida Institute of Technology, Melbourne, Florida
Office Address
442 RRB
Vanderbilt University Medical Center
Nashville, FL 37232-6600
Research Description
Our research group is focused on understanding the roles of specific lipid molecular species and phospholipases in cellular functions and human disease. The laboratory is highly interdisciplinary with research faculty, postdoctoral fellows, staff and graduate students coming from a variety of backgrounds including chemistry, biochemistry, pharmacology, and mathematics. We combine systems biology approaches with mass spectrometry to profile changes in cellular lipid species (lipidomics). Much of our work is focused on understanding the role of phospholipase D (PLD) in growth factor and G protein coupled receptor (GPCR) signaling networks. With our collaborators we are developing novel chemical inhibitors of PLD and ancillaryproteins in the signaling network to better understand the role of PLD in cancers and tumorigenesis, as well as to explore its therapeutic potential. Areas of current research include: structure and enzymology of PLD; defining the signal transduction networks from the receptor tyrosine kinases and GPCRs to PLD; mechanisms by which phosphatidic acid (PA) regulates cell cycle, growth, and proliferation; roles of PLD in zebrafish; developing novel probes for defining specific lipid binding targets; precursor-product relationships of lipid enzymes using mass spectrometry; lipid peroxidation and antioxidant mechanisms; and advanced methods in lipid mass spectrometry.

A complete listing of our publications can be found at: http://www.ncbi.nlm.nih.gov/sites/myncbi/h..brown.1/bibliography/41176006/public/?sort=date&direction=descending

Research Keywords
Research in Pharmacology, Biochemistry, Bioorganic, and Analytical Chemistry.
Mathews TP, Hill S, Rose KL, Ivanova PT, Lindsley CW, Brown HA. Human phospholipase D activity transiently regulates pyrimidine biosynthesis in malignant gliomas. ACS Chem. Biol [print-electronic]. 2015 May 5/15/2015; 10(5): 1258-68. PMID: 25646564, PMCID: PMC4433586, DOI: 10.1021/cb500772c, ISSN: 1554-8937.

Gorden DL, Myers DS, Ivanova PT, Fahy E, Maurya MR, Gupta S, Min J, Spann NJ, McDonald JG, Kelly SL, Duan J, Sullards MC, Leiker TJ, Barkley RM, Quehenberger O, Armando AM, Milne SB, Mathews TP, Armstrong MD, Li C, Melvin WV, Clements RH, Washington MK, Mendonsa AM, Witztum JL, Guan Z, Glass CK, Murphy RC, Dennis EA, Merrill AH, Russell DW, Subramaniam S, Brown HA. Biomarkers of NAFLD progression: a lipidomics approach to an epidemic. J. Lipid Res [print-electronic]. 2015 Mar; 56(3): 722-36. PMID: 25598080, PMCID: PMC4340319, PII: jlr.P056002, DOI: 10.1194/jlr.P056002, ISSN: 0022-2275.

Scott SA, Spencer CT, O'Reilly MC, Brown KA, Lavieri RR, Cho CH, Jung DI, Larock RC, Brown HA, Lindsley CW. Discovery of desketoraloxifene analogues as inhibitors of mammalian, Pseudomonas aeruginosa, and NAPE phospholipase D enzymes. ACS Chem. Biol [print-electronic]. 2015 Feb 2/20/2015; 10(2): 421-32. PMID: 25384256, PMCID: PMC4336625, DOI: 10.1021/cb500828m, ISSN: 1554-8937.

Spencer C, Brown HA. Biochemical characterization of a Pseudomonas aeruginosa phospholipase D. Biochemistry [print-electronic]. 2015 Feb 2/10/2015; 54(5): 1208-18. PMID: 25565226, PMCID: PMC4337821, DOI: 10.1021/bi501291t, ISSN: 1520-4995.

Warrier M, Shih DM, Burrows AC, Ferguson D, Gromovsky AD, Brown AL, Marshall S, McDaniel A, Schugar RC, Wang Z, Sacks J, Rong X, Vallim TA, Chou J, Ivanova PT, Myers DS, Brown HA, Lee RG, Crooke RM, Graham MJ, Liu X, Parini P, Tontonoz P, Lusis AJ, Hazen SL, Temel RE, Brown JM. The TMAO-Generating Enzyme Flavin Monooxygenase 3 Is a Central Regulator of Cholesterol Balance. Cell Rep [print-electronic]. 2015 Jan 1/14/2015; PMID: 25600868, PMCID: PMC4501903, PII: S2211-1247(14)01065-1, DOI: 10.1016/j.celrep.2014.12.036, ISSN: 2211-1247.

Taylor, HE, Simmons, GE, Mathews, TP, Khatua, AK, Popik, W., Lindsley, CW, D'Aquila, RT, Brown, HA.. Phospholipase D1 couples CD4+T cell activation to c-Myc-dependent deoxyribonucleotide pool expansion and HIV-1 replication. 2015.

McDonald, JG, Ivanova, PT, Brown, HA.. Approaches to lipid analysis. 2015.

Ivanova PT, Myers DS, Milne SB, McClaren JL, Thomas PG, Brown HA. Lipid composition of viral envelope of three strains of influenza virus-not all viruses are created equal. 2015.

O'Reilly MC, Scott SA, Brown HA, Lindsley CW. Further evaluation of novel structural modifications to scaffolds that engender PLD isoform selective inhibition. Bioorg. Med. Chem. Lett [print-electronic]. 2014 Dec 12/15/2014; 24(24): 5553-7. PMID: 25466173, PMCID: PMC4535313, PII: S0960-894X(14)01188-3, DOI: 10.1016/j.bmcl.2014.11.017, ISSN: 1464-3405.

O'Reilly MC, Oguin TH, Scott SA, Thomas PG, Locuson CW, Morrison RD, Daniels JS, Brown HA, Lindsley CW. Discovery of a highly selective PLD2 inhibitor (ML395): a new probe with improved physiochemical properties and broad-spectrum antiviral activity against influenza strains. ChemMedChem [print-electronic]. 2014 Dec; 9(12): 2633-7. PMID: 25210004, PMCID: PMC4535312, DOI: 10.1002/cmdc.201402333, ISSN: 1860-7187.

Bruntz RC, Lindsley CW, Brown HA. Phospholipase D signaling pathways and phosphatidic acid as therapeutic targets in cancer. Pharmacol. Rev. 2014 Oct; 66(4): 1033-79. PMID: 25244928, PMCID: PMC4180337, PII: 66/4/1033, DOI: 10.1124/pr.114.009217, ISSN: 1521-0081.

Oguin TH, Sharma S, Stuart AD, Duan S, Scott SA, Jones CK, Daniels JS, Lindsley CW, Thomas PG, Brown HA. Phospholipase D facilitates efficient entry of influenza virus, allowing escape from innate immune inhibition. J. Biol. Chem [print-electronic]. 2014 Sep 9/12/2014; 289(37): 25405-17. PMID: 25065577, PMCID: PMC4162146, PII: M114.558817, DOI: 10.1074/jbc.M114.558817, ISSN: 1083-351X.

Scott SA, Mathews TP, Ivanova PT, Lindsley CW, Brown HA. Chemical modulation of glycerolipid signaling and metabolic pathways. Biochim. Biophys. Acta [print-electronic]. 2014 Aug; 1841(8): 1060-84. PMID: 24440821, PMCID: PMC4069240, PII: S1388-1981(14)00014-6, DOI: 10.1016/j.bbalip.2014.01.009, ISSN: 0006-3002.

Cipriano R, Bryson BL, Miskimen KL, Bartel CA, Hernandez-Sanchez W, Bruntz RC, Scott SA, Lindsley CW, Brown HA, Jackson MW. Hyperactivation of EGFR and downstream effector phospholipase D1 by oncogenic FAM83B. Oncogene [print-electronic]. 2014 Jun 6/19/2014; 33(25): 3298-306. PMID: 23912460, PMCID: PMC3923847, PII: onc2013293, DOI: 10.1038/onc.2013.293, ISSN: 1476-5594.

Leamy AK, Egnatchik RA, Shiota M, Ivanova PT, Myers DS, Brown HA, Young JD. Enhanced synthesis of saturated phospholipids is associated with ER stress and lipotoxicity in palmitate treated hepatic cells. J. Lipid Res [print-electronic]. 2014 May 5/23/2014; 55(7): 1478-88. PMID: 24859739, PMCID: PMC4076085, PII: jlr.M050237, DOI: 10.1194/jlr.M050237, ISSN: 0022-2275.

Ren J, Pei-Chen Lin C, Pathak MC, Temple BR, Nile AH, Mousley CJ, Duncan MC, Eckert DM, Leiker TJ, Ivanova PT, Myers DS, Murphy RC, Brown HA, Verdaasdonk J, Bloom KS, Ortlund EA, Neiman AM, Bankaitis VA. A phosphatidylinositol transfer protein integrates phosphoinositide signaling with lipid droplet metabolism to regulate a developmental program of nutrient stress-induced membrane biogenesis. Mol. Biol. Cell [print-electronic]. 2014 Mar; 25(5): 712-27. PMID: 24403601, PMCID: PMC3937096, PII: mbc.E13-11-0634, DOI: 10.1091/mbc.E13-11-0634, ISSN: 1939-4586.

Bruntz RC, Taylor HE, Lindsley CW, Brown HA. Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells. J. Biol. Chem [print-electronic]. 2014 Jan 1/10/2014; 289(2): 600-16. PMID: 24257753, PMCID: PMC3887188, PII: M113.532978, DOI: 10.1074/jbc.M113.532978, ISSN: 1083-351X.

Faust JE, Manisundaram A, Ivanova PT, Milne SB, Summerville JB, Brown HA, Wangler M, Stern M, McNew JA. Peroxisomes are required for lipid metabolism and muscle function in Drosophila melanogaster. PLoS ONE. 2014; 9(6): e100213. PMID: 24945818, PMCID: PMC4063865, PII: PONE-D-14-09238, DOI: 10.1371/journal.pone.0100213, ISSN: 1932-6203.

Shibata N, Carlin AF, Spann NJ, Saijo K, Morello CS, McDonald JG, Romanoski CE, Maurya MR, Kaikkonen MU, Lam MT, Crotti A, Reichart D, Fox JN, Quehenberger O, Raetz CR, Sullards MC, Murphy RC, Merrill AH, Brown HA, Dennis EA, Fahy E, Subramaniam S, Cavener DR, Spector DH, Russell DW, Glass CK. 25-Hydroxycholesterol activates the integrated stress response to reprogram transcription and translation in macrophages. J. Biol. Chem [print-electronic]. 2013 Dec 12/13/2013; 288(50): 35812-23. PMID: 24189069, PMCID: PMC3861632, PII: M113.519637, DOI: 10.1074/jbc.M113.519637, ISSN: 1083-351X.

Shulga YV, Loukov D, Ivanova PT, Milne SB, Myers DS, Hatch GM, Umeh G, Jalan D, Fullerton MD, Steinberg GR, Topham MK, Brown HA, Epand RM. Diacylglycerol kinase delta promotes lipogenesis. Biochemistry [print-electronic]. 2013 Nov 11/5/2013; 52(44): 7766-76. PMID: 24090246, PMCID: PMC3864584, DOI: 10.1021/bi401178y, ISSN: 1520-4995.

Thomas G, Betters JL, Lord CC, Brown AL, Marshall S, Ferguson D, Sawyer J, Davis MA, Melchior JT, Blume LC, Howlett AC, Ivanova PT, Milne SB, Myers DS, Mrak I, Leber V, Heier C, Taschler U, Blankman JL, Cravatt BF, Lee RG, Crooke RM, Graham MJ, Zimmermann R, Brown HA, Brown JM. The serine hydrolase ABHD6 Is a critical regulator of the metabolic syndrome. Cell Rep [print-electronic]. 2013 Oct 10/31/2013; 5(2): 508-20. PMID: 24095738, PMCID: PMC3833083, PII: S2211-1247(13)00507-X, DOI: 10.1016/j.celrep.2013.08.047, ISSN: 2211-1247.

Maurya MR, Gupta S, Li X, Fahy E, Dinasarapu AR, Sud M, Brown HA, Glass CK, Murphy RC, Russell DW, Dennis EA, Subramaniam S. Analysis of inflammatory and lipid metabolic networks across RAW264.7 and thioglycolate-elicited macrophages. J. Lipid Res [print-electronic]. 2013 Sep; 54(9): 2525-42. PMID: 23776196, PMCID: PMC3735949, PII: jlr.M040212, DOI: 10.1194/jlr.M040212, ISSN: 0022-2275.

Scott SA, Xiang Y, Mathews TP, Cho HP, Myers DS, Armstrong MD, Tallman KA, O'Reilly MC, Lindsley CW, Brown HA. Regulation of phospholipase D activity and phosphatidic acid production after purinergic (P2Y6) receptor stimulation. J. Biol. Chem [print-electronic]. 2013 Jul 7/12/2013; 288(28): 20477-87. PMID: 23723068, PMCID: PMC3711313, PII: M113.451708, DOI: 10.1074/jbc.M113.451708, ISSN: 1083-351X.

Milne SB, Mathews TP, Myers DS, Ivanova PT, Brown HA. Sum of the parts: mass spectrometry-based metabolomics. Biochemistry [print-electronic]. 2013 Jun 6/4/2013; 52(22): 3829-40. PMID: 23442130, PMCID: PMC3674197, DOI: 10.1021/bi400060e, ISSN: 1520-4995.

Kim SH, Scott SA, Bennett MJ, Carson RP, Fessel J, Brown HA, Ess KC. Multi-organ abnormalities and mTORC1 activation in zebrafish model of multiple acyl-CoA dehydrogenase deficiency. PLoS Genet [print-electronic]. 2013 Jun; 9(6): e1003563. PMID: 23785301, PMCID: PMC3681725, PII: PGENETICS-D-12-02748, DOI: 10.1371/journal.pgen.1003563, ISSN: 1553-7404.

Yang MY, Hilton MB, Seaman S, Haines DC, Nagashima K, Burks CM, Tessarollo L, Ivanova PT, Brown HA, Umstead TM, Floros J, Chroneos ZC, St Croix B. Essential regulation of lung surfactant homeostasis by the orphan G protein-coupled receptor GPR116. Cell Rep [print-electronic]. 2013 May 5/30/2013; 3(5): 1457-64. PMID: 23684610, PMCID: PMC3695742, PII: S2211-1247(13)00201-5, DOI: 10.1016/j.celrep.2013.04.019, ISSN: 2211-1247.

O'Reilly MC, Scott SA, Brown KA, Oguin TH, Thomas PG, Daniels JS, Morrison R, Brown HA, Lindsley CW. Development of dual PLD1/2 and PLD2 selective inhibitors from a common 1,3,8-Triazaspiro[4.5]decane Core: discovery of Ml298 and Ml299 that decrease invasive migration in U87-MG glioblastoma cells. J. Med. Chem [print-electronic]. 2013 Mar 3/28/2013; 56(6): 2695-9. PMID: 23445448, PMCID: PMC3632306, DOI: 10.1021/jm301782e, ISSN: 1520-4804.

Tallman KA, Armstrong MD, Milne SB, Marnett LJ, Brown HA, Porter NA. Cobalt carbonyl complexes as probes for alkyne-tagged lipids. J. Lipid Res [print-electronic]. 2013 Mar; 54(3): 859-68. PMID: 23307946, PMCID: PMC3617960, PII: jlr.D033332, DOI: 10.1194/jlr.D033332, ISSN: 0022-2275.

Meszaros P, Klappe K, van Dam A, Ivanova PT, Milne SB, Myers DS, Brown HA, Permentier H, Hoekstra D, Kok JW. Long term myriocin treatment increases MRP1 transport activity. Int. J. Biochem. Cell Biol [print-electronic]. 2013 Feb; 45(2): 326-34. PMID: 23178537, PMCID: PMC3826170, PII: S1357-2725(12)00372-X, DOI: 10.1016/j.biocel.2012.11.009, ISSN: 1878-5875.

Moore,J., Li,S., Myers, DS, Milne, SB, Brown, HA, and Shaw, W.. Standardization and quantification of lysophosphatidic acid compounds by normal-phase and reverse-phase chromatography-tandem mass spectrometry. 2013.

Li JZ, Huang Y, Karaman R, Ivanova PT, Brown HA, Roddy T, Castro-Perez J, Cohen JC, Hobbs HH. Chronic overexpression of PNPLA3I148M in mouse liver causes hepatic steatosis. J. Clin. Invest. 2012 Nov; 122(11): 4130-44. PMID: 23023705, PMCID: PMC3484461, PII: 65179, DOI: 10.1172/JCI65179, ISSN: 1558-8238.

Spann NJ, Garmire LX, McDonald JG, Myers DS, Milne SB, Shibata N, Reichart D, Fox JN, Shaked I, Heudobler D, Raetz CR, Wang EW, Kelly SL, Sullards MC, Murphy RC, Merrill AH, Brown HA, Dennis EA, Li AC, Ley K, Tsimikas S, Fahy E, Subramaniam S, Quehenberger O, Russell DW, Glass CK. Regulated accumulation of desmosterol integrates macrophage lipid metabolism and inflammatory responses. Cell. 2012 Sep 9/28/2012; 151(1): 138-52. PMID: 23021221, PMCID: PMC3464914, PII: S0092-8674(12)01059-8, DOI: 10.1016/j.cell.2012.06.054, ISSN: 1097-4172.

Cipriano R, Graham J, Miskimen KL, Bryson BL, Bruntz RC, Scott SA, Brown HA, Stark GR, Jackson MW. FAM83B mediates EGFR- and RAS-driven oncogenic transformation. J. Clin. Invest [print-electronic]. 2012 Sep; 122(9): 3197-210. PMID: 22886302, PMCID: PMC3428078, PII: 60517, DOI: 10.1172/JCI60517, ISSN: 1558-8238.

Kumari M, Schoiswohl G, Chitraju C, Paar M, Cornaciu I, Rangrez AY, Wongsiriroj N, Nagy HM, Ivanova PT, Scott SA, Knittelfelder O, Rechberger GN, Birner-Gruenberger R, Eder S, Brown HA, Haemmerle G, Oberer M, Lass A, Kershaw EE, Zimmermann R, Zechner R. Adiponutrin functions as a nutritionally regulated lysophosphatidic acid acyltransferase. Cell Metab. 2012 May 5/2/2012; 15(5): 691-702. PMID: 22560221, PMCID: PMC3361708, PII: S1550-4131(12)00148-9, DOI: 10.1016/j.cmet.2012.04.008, ISSN: 1932-7420.

Lord CC, Betters JL, Ivanova PT, Milne SB, Myers DS, Madenspacher J, Thomas G, Chung S, Liu M, Davis MA, Lee RG, Crooke RM, Graham MJ, Parks JS, Brasaemle DL, Fessler MB, Brown HA, Brown JM. CGI-58/ABHD5-derived signaling lipids regulate systemic inflammation and insulin action. Diabetes [print-electronic]. 2012 Feb; 61(2): 355-63. PMID: 22228714, PMCID: PMC3266405, PII: db11-0994, DOI: 10.2337/db11-0994, ISSN: 1939-327X.

Lindsley CW, Brown HA. Phospholipase D as a therapeutic target in brain disorders. Neuropsychopharmacology. 2012 Jan; 37(1): 301-2. PMID: 22157867, PMCID: PMC3238067, PII: npp2011178, DOI: 10.1038/npp.2011.178, ISSN: 1740-634X.

Brown HA. Lipidomics: When apocrypha becomes canonical. Current Opin Chem Biology. 2012; 16((1-2)): 221-6.

Myers DS, Ivanova PT, Milne SB, Brown HA. Quantitative analysis of glycerophospholipids by LC-MS: acquisition, data handling, and interpretation. Biochim. Biophys. Acta [print-electronic]. 2011 Nov; 1811(11): 748-57. PMID: 21683157, PMCID: PMC3292045, PII: S1388-1981(11)00082-5, DOI: 10.1016/j.bbalip.2011.05.015, ISSN: 0006-3002.

Selvy PE, Lavieri RR, Lindsley CW, Brown HA. Phospholipase D: enzymology, functionality, and chemical modulation. Chem. Rev [print-electronic]. 2011 Oct 10/12/2011; 111(10): 6064-119. PMID: 21936578, PMCID: PMC3233269, DOI: 10.1021/cr200296t, ISSN: 1520-6890.

Brown HA, Marnett LJ. Introduction to lipid biochemistry, metabolism, and signaling [editorial]. Chem. Rev [print-electronic]. 2011 Oct 10/12/2011; 111(10): 5817-20. PMID: 21951202, DOI: 10.1021/cr200363s, ISSN: 1520-6890.

Liu ST, Sharon-Friling R, Ivanova P, Milne SB, Myers DS, Rabinowitz JD, Brown HA, Shenk T. Synaptic vesicle-like lipidome of human cytomegalovirus virions reveals a role for SNARE machinery in virion egress. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2011 Aug 8/2/2011; 108(31): 12869-74. PMID: 21768361, PMCID: PMC3150939, PII: 1109796108, DOI: 10.1073/pnas.1109796108, ISSN: 1091-6490.

Norton LJ, Zhang Q, Saqib KM, Schrewe H, Macura K, Anderson KE, Lindsley CW, Brown HA, Rudge SA, Wakelam MJ. PLD1 rather than PLD2 regulates phorbol-ester-, adhesion-dependent and Fc{gamma}-receptor-stimulated ROS production in neutrophils. J. Cell. Sci [print-electronic]. 2011 Jun 6/15/2011; 124(Pt 12): 1973-83. PMID: 21610093, PMCID: PMC3104032, PII: jcs.082008, DOI: 10.1242/jcs.082008, ISSN: 1477-9137.

Kang DW, Park MH, Lee YJ, Kim HS, Lindsley CW, Brown HA, Min DS. Autoregulation of phospholipase D activity is coupled to selective induction of phospholipase D1 expression to promote invasion of breast cancer cells. 2011.

Gorden DL, Ivanova PT, Myers DS, McIntyre JO, VanSaun MN, Wright JK, Matrisian LM, Brown HA. Increased diacylglycerols characterize hepatic lipid changes in progression of human nonalcoholic fatty liver disease; comparison to a murine model. PLoS ONE [print-electronic]. 2011; 6(8): e22775. PMID: 21857953, PMCID: PMC3153459, PII: PONE-D-11-04361, DOI: 10.1371/journal.pone.0022775, ISSN: 1932-6203.

Dennis EA, Deems RA, Harkewicz R, Quehenberger O, Brown HA, Milne SB, Myers DS, Glass CK, Hardiman G, Reichart D, Merrill AH, Sullards MC, Wang E, Murphy RC, Raetz CR, Garrett TA, Guan Z, Ryan AC, Russell DW, McDonald JG, Thompson BM, Shaw WA, Sud M, Zhao Y, Gupta S, Maurya MR, Fahy E, Subramaniam S. A mouse macrophage lipidome. J. Biol. Chem [print-electronic]. 2010 Dec 12/17/2010; 285(51): 39976-85. PMID: 20923771, PMCID: PMC3000979, PII: M110.182915, DOI: 10.1074/jbc.M110.182915, ISSN: 1083-351X.

Lavieri RR, Scott SA, Selvy PE, Kim K, Jadhav S, Morrison RD, Daniels JS, Brown HA, Lindsley CW. Design, synthesis, and biological evaluation of halogenated N-(2-(4-oxo-1-phenyl-1,3,8-triazaspiro[4.5]decan-8-yl)ethyl)benzamides: discovery of an isoform-selective small molecule phospholipase D2 inhibitor. J. Med. Chem. 2010 Sep 9/23/2010; 53(18): 6706-19. PMID: 20735042, PMCID: PMC3179181, DOI: 10.1021/jm100814g, ISSN: 1520-4804.

Klappe K, Dijkhuis AJ, Hummel I, van Dam A, Ivanova PT, Milne SB, Myers DS, Brown HA, Permentier H, Kok JW. Extensive sphingolipid depletion does not affect lipid raft integrity or lipid raft localization and efflux function of the ABC transporter MRP1. Biochem. J. 2010 Sep 9/15/2010; 430(3): 519-29. PMID: 20604746, PMCID: PMC3791605, PII: BJ20091882, DOI: 10.1042/BJ20091882, ISSN: 1470-8728.

Andreyev AY, Fahy E, Guan Z, Kelly S, Li X, McDonald JG, Milne S, Myers D, Park H, Ryan A, Thompson BM, Wang E, Zhao Y, Brown HA, Merrill AH, Raetz CR, Russell DW, Subramaniam S, Dennis EA. Subcellular organelle lipidomics in TLR-4-activated macrophages. J. Lipid Res [print-electronic]. 2010 Sep; 51(9): 2785-97. PMID: 20574076, PMCID: PMC2918461, PII: jlr.M008748, DOI: 10.1194/jlr.M008748, ISSN: 0022-2275.

Ivanova PT, Milne SB, Brown HA. Identification of atypical ether-linked glycerophospholipid species in macrophages by mass spectrometry. J. Lipid Res [print-electronic]. 2010 Jun; 51(6): 1581-90. PMID: 19965583, PMCID: PMC3035522, PII: jlr.D003715, DOI: 10.1194/jlr.D003715, ISSN: 0022-2275.

Milne SB, Tallman KA, Serwa R, Rouzer CA, Armstrong MD, Marnett LJ, Lukehart CM, Porter NA, Brown HA. Capture and release of alkyne-derivatized glycerophospholipids using cobalt chemistry. Nat. Chem. Biol [print-electronic]. 2010 Mar; 6(3): 205-7. PMID: 20098428, PMCID: PMC2822082, PII: nchembio.311, DOI: 10.1038/nchembio.311, ISSN: 1552-4469.

Shulga YV, Myers DS, Ivanova PT, Milne SB, Brown HA, Topham MK, Epand RM. Molecular species of phosphatidylinositol-cycle intermediates in the endoplasmic reticulum and plasma membrane. Biochemistry. 2010 Jan 1/19/2010; 49(2): 312-7. PMID: 20000336, PMCID: PMC2805767, DOI: 10.1021/bi901551e, ISSN: 1520-4995.

Callender HL, Horn MA, DeCamp DL, Sternweis PC, Brown HA. Modeling species-specific diacylglycerol dynamics in the RAW 264.7 macrophage. 2010.

Quehenberger O, Armando AM, Brown HA, Milne SB, Myers DS, Merrill AH, Bandyopadhyay S, Jones KN, Kelly S, Shaner RL, Sullards CM, Wang E, Murphy RC et al.. Lipidomics reveals remarkable diversity of lipids in human plasma. 2010.

Ivanova PT, Milne SB, Myers DS, Brown HA. Lipidomics: a mass spectrometry based systems level analysis of cellular lipids. Curr Opin Chem Biol [print-electronic]. 2009 Dec; 13(5-6): 526-31. PMID: 19744877, PMCID: PMC2787871, PII: S1367-5931(09)00109-4, DOI: 10.1016/j.cbpa.2009.08.011, ISSN: 1879-0402.

Lung M, Shulga YV, Ivanova PT, Myers DS, Milne SB, Brown HA, Topham MK, Epand RM. Diacylglycerol kinase epsilon is selective for both acyl chains of phosphatidic acid or diacylglycerol. J. Biol. Chem [print-electronic]. 2009 Nov 11/6/2009; 284(45): 31062-73. PMID: 19744926, PMCID: PMC2781506, PII: M109.050617, DOI: 10.1074/jbc.M109.050617, ISSN: 1083-351X.

Rappley I, Myers DS, Milne SB, Ivanova PT, Lavoie MJ, Brown HA, Selkoe DJ. Lipidomic profiling in mouse brain reveals differences between ages and genders, with smaller changes associated with alpha-synuclein genotype. J. Neurochem [print-electronic]. 2009 Oct; 111(1): 15-25. PMID: 19627450, PMCID: PMC2752313, PII: JNC6290, DOI: 10.1111/j.1471-4159.2009.06290.x, ISSN: 1471-4159.

Brown HA, Murphy RC. Working towards an exegesis for lipids in biology. Nat. Chem. Biol. 2009 Sep; 5(9): 602-6. PMID: 19690530, PMCID: PMC3785062, PII: nchembio0909-602, DOI: 10.1038/nchembio0909-602, ISSN: 1552-4469.

Holinstat M, Preininger AM, Milne SB, Hudson WJ, Brown HA, Hamm HE. Irreversible platelet activation requires protease-activated receptor 1-mediated signaling to phosphatidylinositol phosphates. Mol. Pharmacol [print-electronic]. 2009 Aug; 76(2): 301-13. PMID: 19483102, PMCID: PMC2713123, PII: mol.109.056622, DOI: 10.1124/mol.109.056622, ISSN: 1521-0111.

Zeng XX, Zheng X, Xiang Y, Cho HP, Jessen JR, Zhong TP, Solnica-Krezel L, Brown HA. Phospholipase D1 is required for angiogenesis of intersegmental blood vessels in zebrafish. Dev. Biol [print-electronic]. 2009 Apr 4/15/2009; 328(2): 363-76. PMID: 19389349, PMCID: PMC2688691, PII: S0012-1606(09)00089-X, DOI: 10.1016/j.ydbio.2009.01.035, ISSN: 1095-564X.

Lewis JA, Scott SA, Lavieri R, Buck JR, Selvy PE, Stoops SL, Armstrong MD, Brown HA, Lindsley CW. Design and synthesis of isoform-selective phospholipase D (PLD) inhibitors. Part I: Impact of alternative halogenated privileged structures for PLD1 specificity. Bioorg. Med. Chem. Lett [print-electronic]. 2009 Apr 4/1/2009; 19(7): 1916-20. PMID: 19268584, PMCID: PMC3791604, PII: S0960-894X(09)00211-X, DOI: 10.1016/j.bmcl.2009.02.057, ISSN: 1464-3405.

Marlo JE, Niswender CM, Days EL, Bridges TM, Xiang Y, Rodriguez AL, Shirey JK, Brady AE, Nalywajko T, Luo Q, Austin CA, Williams MB, Kim K, Williams R, Orton D, Brown HA, Lindsley CW, Weaver CD, Conn PJ. Discovery and characterization of novel allosteric potentiators of M1 muscarinic receptors reveals multiple modes of activity. Mol. Pharmacol [print-electronic]. 2009 Mar; 75(3): 577-88. PMID: 19047481, PMCID: PMC2684909, PII: mol.108.052886, DOI: 10.1124/mol.108.052886, ISSN: 1521-0111.

Scott SA, Selvy PE, Buck JR, Cho HP, Criswell TL, Thomas AL, Armstrong MD, Arteaga CL, Lindsley CW, Brown HA. Design of isoform-selective phospholipase D inhibitors that modulate cancer cell invasiveness. Nat. Chem. Biol [print-electronic]. 2009 Feb; 5(2): 108-17. PMID: 19136975, PMCID: PMC3798018, PII: nchembio.140, DOI: 10.1038/nchembio.140, ISSN: 1552-4469.

Lavieri R, Scott SA, Lewis JA, Selvy PE, Armstrong MD, Brown HA, Lindsley CW. Design and synthesis of isoform-selective phospholipase D (PLD) inhibitors. Part II. Identification of the 1,3,8-triazaspiro[4,5]-decan-4-one privileged structure that engenders PLD2 selectivity. 2009.

Burnum KE, Cornett DS, Puolitaival SM, Milne SB, Myers DS, Tranguch S, Brown HA, Dey SK, Caprioli RM. Spatial and temporal alterations of phospholipids determined by mass spectrometry during mouse embryo implantation. 2009.

Rappley I, Myers DS, Milne SB, Ivanova PT, LaVoie J, Brown HA, Selkoe DJ. Lipidomics profiling in mouse brain reveals differences between ages and genders with smaller changes associated with alfa-synuclein genotype. 2009.

Brown HA. New mechanism for a classic folate inhibitor. Nat. Chem. Biol. 2008 Oct; 4(10): 581-2. PMID: 18800042, PII: nchembio1008-581, DOI: 10.1038/nchembio1008-581, ISSN: 1552-4469.

Milne SB, Ivanova PT, Armstrong MD, Myers DS, Lubarda J, Shulga YV, Topham MK, Brown HA, Epand RM. Dramatic differences in the roles in lipid metabolism of two isoforms of diacylglycerol kinase. Biochemistry [print-electronic]. 2008 Sep 9/9/2008; 47(36): 9372-9. PMID: 18702510, PMCID: PMC2744458, DOI: 10.1021/bi800492c, ISSN: 1520-4995.

Garcia A, Zheng Y, Zhao C, Toschi A, Fan J, Shraibman N, Brown HA, Bar-Sagi D, Foster DA, Arbiser JL. Honokiol suppresses survival signals mediated by Ras-dependent phospholipase D activity in human cancer cells. Clin. Cancer Res. 2008 Jul 7/1/2008; 14(13): 4267-74. PMID: 18594009, PMCID: PMC2759181, PII: 14/13/4267, DOI: 10.1158/1078-0432.CCR-08-0102, ISSN: 1078-0432.

Sun Y, Fang Y, Yoon MS, Zhang C, Roccio M, Zwartkruis FJ, Armstrong M, Brown HA, Chen J. Phospholipase D1 is an effector of Rheb in the mTOR pathway. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2008 Jun 6/17/2008; 105(24): 8286-91. PMID: 18550814, PMCID: PMC2448829, PII: 0712268105, DOI: 10.1073/pnas.0712268105, ISSN: 1091-6490.

Murphy SC, Fernandez-Pol S, Chung PH, Prasanna Murthy SN, Milne SB, Salomao M, Brown HA, Lomasney JW, Mohandas N, Haldar K. Cytoplasmic remodeling of erythrocyte raft lipids during infection by the human malaria parasite Plasmodium falciparum. Blood [print-electronic]. 2007 Sep 9/15/2007; 110(6): 2132-9. PMID: 17526861, PMCID: PMC1976375, PII: blood-2007-04-083873, DOI: 10.1182/blood-2007-04-083873, ISSN: 0006-4971.

Rouzer CA, Ivanova PT, Byrne MO, Brown HA, Marnett LJ. Lipid profiling reveals glycerophospholipid remodeling in zymosan-stimulated macrophages. Biochemistry [print-electronic]. 2007 May 5/22/2007; 46(20): 6026-42. PMID: 17458939, DOI: 10.1021/bi0621617, ISSN: 0006-2960.

Callender HL, Forrester JS, Ivanova P, Preininger A, Milne S, Brown HA. Quantification of diacylglycerol species from cellular extracts by electrospray ionization mass spectrometry using a linear regression algorithm. Anal. Chem. 2007 Jan 1/1/2007; 79(1): 263-72. PMID: 17194150, DOI: 10.1021/ac061083q, ISSN: 0003-2700.

Sud M, Fahy E, Cotter D, Brown A, Dennis EA, Glass CK, Merrill AH, Murphy RC, Raetz CR, Russell DW, Subramaniam S. LMSD: LIPID MAPS structure database. Nucleic Acids Res [print-electronic]. 2007 Jan; 35(Database issue): D527-32. PMID: 17098933, PMCID: PMC1669719, PII: gkl838, DOI: 10.1093/nar/gkl838, ISSN: 1362-4962.

Brown HA, Henage LG, Preininger AM, Xiang Y, Exton JH. Biochemical analysis of phospholipase D. Meth. Enzymol. 2007; 434: 49-87. PMID: 17954242, PII: S0076-6879(07)34004-4, DOI: 10.1016/S0076-6879(07)34004-4, ISSN: 0076-6879.

Ivanova PT, Milne SB, Byrne MO, Xiang Y, Brown HA. Glycerophospholipid identification and quantitation by electrospray ionization mass spectrometry. Meth. Enzymol. 2007; 432: 21-57. PMID: 17954212, PII: S0076-6879(07)32002-8, DOI: 10.1016/S0076-6879(07)32002-8, ISSN: 0076-6879.

Rouzer CA, Ivanova PT, Byrne MO, Milne SB, Marnett LJ, Brown HA. Lipid profiling reveals arachidonate deficiency in RAW264.7 cells: Structural and functional implications. Biochemistry. 2006 Dec 12/12/2006; 45(49): 14795-808. PMID: 17144673, PMCID: PMC2443946, DOI: 10.1021/bi061723j, ISSN: 0006-2960.

Preininger AM, Henage LG, Oldham WM, Yoon EJ, Hamm HE, Brown HA. Direct modulation of phospholipase D activity by Gbetagamma. Mol. Pharmacol [print-electronic]. 2006 Jul; 70(1): 311-8. PMID: 16638972, PII: mol.105.021451, DOI: 10.1124/mol.105.021451, ISSN: 0026-895X.

Milne S, Ivanova P, Forrester J, Alex Brown H. Lipidomics: an analysis of cellular lipids by ESI-MS. Methods. 2006 Jun; 39(2): 92-103. PMID: 16846739, PII: S1046-2023(06)00073-9, DOI: 10.1016/j.ymeth.2006.05.014, ISSN: 1046-2023.

Henage LG, Exton JH, Brown HA. Kinetic analysis of a mammalian phospholipase D: allosteric modulation by monomeric GTPases, protein kinase C, and polyphosphoinositides. J. Biol. Chem [print-electronic]. 2006 Feb 2/10/2006; 281(6): 3408-17. PMID: 16339153, PMCID: PMC3800466, PII: M508800200, DOI: 10.1074/jbc.M508800200, ISSN: 0021-9258.

Milne, S.B., Forrester, J.S., Ivanova, P.T., and Brown H.A.. Functional Lipidomics. Eds L.Feng, GD Prestwich. CRC Press, Boca Raton, FL. [place unknown: publisher unknown]; 2006.

Milne SB, Ivanova PT, DeCamp D, Hsueh RC, Brown HA. A targeted mass spectrometric analysis of phosphatidylinositol phosphate species. J. Lipid Res [print-electronic]. 2005 Aug; 46(8): 1796-802. PMID: 15897608, PII: D500010-JLR200, DOI: 10.1194/jlr.D500010-JLR200, ISSN: 0022-2275.

Dennis, E.A., Brown, H.A., Merrill, A.H., Murphy, R.C., Raetz, C.R., Russell, D.W., Wooley, J. and Deems, R.A. The LIPID MAPS Approach to Lipidomics. In: Functional Lipidomics. edited by L. Feng and G.D. Prestwich. CRC Press, Boca Raton, FL. 2005.

Ivanova PT, Milne SB, Forrester JS, Brown HA. LIPID arrays: new tools in the understanding of membrane dynamics and lipid signaling. Mol. Interv. 2004 Apr; 4(2): 86-96. PMID: 15087482, PII: 4/2/86, DOI: 10.1124/mi.4.2.6, ISSN: 1543-2548.

Forrester JS, Milne SB, Ivanova PT, Brown HA. Computational lipidomics: a multiplexed analysis of dynamic changes in membrane lipid composition during signal transduction. Mol. Pharmacol. 2004 Apr; 65(4): 813-21. PMID: 15044609, PII: 65/4/813, DOI: 10.1124/mol.65.4.813, ISSN: 0026-895X.

Walker SJ, Brown HA, . Measurement of G protein-coupled receptor-stimulated phospholipase D activity in intact cells. Methods Mol. Biol. 2004; 237: 89-97. PMID: 14501041, PII: 1-59259-430-1-89, ISSN: 1064-3745.

Fang Y, Park IH, Wu AL, Du G, Huang P, Frohman MA, Walker SJ, Brown HA, Chen J. PLD1 regulates mTOR signaling and mediates Cdc42 activation of S6K1. Curr. Biol. 2003 Dec 12/2/2003; 13(23): 2037-44. PMID: 14653992, PII: S0960982203008637, ISSN: 0960-9822.

Ge M, Gidwani A, Brown HA, Holowka D, Baird B, Freed JH. Ordered and disordered phases coexist in plasma membrane vesicles of RBL-2H3 mast cells. An ESR study. Biophys. J. 2003 Aug; 85(2): 1278-88. PMID: 12885671, PMCID: PMC1303245, PII: S0006-3495(03)74563-9, DOI: 10.1016/S0006-3495(03)74563-9, ISSN: 0006-3495.

Gidwani A, Brown HA, Holowka D, Baird B. Disruption of lipid order by short-chain ceramides correlates with inhibition of phospholipase D and downstream signaling by FcepsilonRI. J. Cell. Sci. 2003 Aug 8/1/2003; 116(Pt 15): 3177-87. PMID: 12829737, PII: 116/15/3177, DOI: 10.1242/jcs.00621, ISSN: 0021-9533.

Sieczkarski SB, Brown HA, Whittaker GR. Role of protein kinase C betaII in influenza virus entry via late endosomes. J. Virol. 2003 Jan; 77(1): 460-9. PMID: 12477851, PMCID: PMC140583, ISSN: 0022-538X.

Milne, S.B., Forrester, J.S., Ivanova, P.T., and Brown, A.H. Multipleaxed Lipid Arrays of Aniti-IgM0induced changes in the glycerophospholipid composition of WEHI-231 cells. AfCS Research Reports. 2003.

Eisen SF, Brown HA. Selective estrogen receptor (ER) modulators differentially regulate phospholipase D catalytic activity in ER-negative breast cancer cells. Mol. Pharmacol. 2002 Oct; 62(4): 911-20. PMID: 12237338, ISSN: 0026-895X.

Walker SJ, Brown HA. Specificity of Rho insert-mediated activation of phospholipase D1. J. Biol. Chem [print-electronic]. 2002 Jul 7/19/2002; 277(29): 26260-7. PMID: 12011045, PII: M201811200, DOI: 10.1074/jbc.M201811200, ISSN: 0021-9258.

Brooks MB, Catalfamo JL, Brown HA, Ivanova P, Lovaglio J. A hereditary bleeding disorder of dogs caused by a lack of platelet procoagulant activity. Blood. 2002 Apr 4/1/2002; 99(7): 2434-41. PMID: 11895776, ISSN: 0006-4971.

Gilman, A.G., et al. Overview of the alliance for cellular signaling. Nature. 2002; 420: 703-6.

Ge M, Cohen JS, Brown HA, Freed JH. ADP ribosylation factor 6 binding to phosphatidylinositol 4,5-bisphosphate-containing vesicles creates defects in the bilayer structure: an electron spin resonance study. Biophys. J. 2001 Aug; 81(2): 994-1005. PMID: 11463641, PMCID: PMC1301569, PII: S0006-3495(01)75757-8, DOI: 10.1016/S0006-3495(01)75757-8, ISSN: 0006-3495.

Ivanova PT, Cerda BA, Horn DM, Cohen JS, McLafferty FW, Brown HA. Electrospray ionization mass spectrometry analysis of changes in phospholipids in RBL-2H3 mastocytoma cells during degranulation. Proc. Natl. Acad. Sci. U.S.A. 2001 Jun 6/19/2001; 98(13): 7152-7. PMID: 11416200, PMCID: PMC34638, PII: 98/13/7152, DOI: 10.1073/pnas.131195098, ISSN: 0027-8424.

Cohen JS, Brown HA. Phospholipases stimulate secretion in RBL mast cells. Biochemistry. 2001 Jun 6/5/2001; 40(22): 6589-97. PMID: 11380253, PII: bi0103011, ISSN: 0006-2960.

Ganley IG, Walker SJ, Manifava M, Li D, Brown HA, Ktistakis NT. Interaction of phospholipase D1 with a casein-kinase-2-like serine kinase. Biochem. J. 2001 Mar 3/1/2001; 354(Pt 2): 369-78. PMID: 11171116, PMCID: PMC1221665, ISSN: 0264-6021.

Walker SJ, Wu WJ, Cerione RA, Brown HA. Activation of phospholipase D1 by Cdc42 requires the Rho insert region. J. Biol. Chem. 2000 May 5/26/2000; 275(21): 15665-8. PMID: 10747870, PII: M000076200, DOI: 10.1074/jbc.M000076200, ISSN: 0021-9258.

Paradiso AM, Brown HA, Ye H, Harden TK, Boucher RC. Heterogeneous responses of cell Ca2+ in human airway epithelium. Exp. Lung Res. 1999 Jun; 25(4): 277-90. PMID: 10378100, ISSN: 0190-2148.

Singer WD, Brown HA, Sternweis PC. Regulation of eukaryotic phosphatidylinositol-specific phospholipase C and phospholipase D. Annu. Rev. Biochem. 1997; 66: 475-509. PMID: 9242915, DOI: 10.1146/annurev.biochem.66.1.475, ISSN: 0066-4154.

Ktistakis NT, Brown HA, Waters MG, Sternweis PC, Roth MG. Evidence that phospholipase D mediates ADP ribosylation factor-dependent formation of Golgi coated vesicles. J. Cell Biol. 1996 Jul; 134(2): 295-306. PMID: 8707816, PMCID: PMC2120869, ISSN: 0021-9525.

Singer WD, Brown HA, Jiang X, Sternweis PC. Regulation of phospholipase D by protein kinase C is synergistic with ADP-ribosylation factor and independent of protein kinase activity. J. Biol. Chem. 1996 Feb 2/23/1996; 271(8): 4504-10. PMID: 8626805, ISSN: 0021-9258.

Lazarowski ER, Watt WC, Stutts MJ, Brown HA, Boucher RC, Harden TK. Enzymatic synthesis of UTP gamma S, a potent hydrolysis resistant agonist of P2U-purinoceptors. Br. J. Pharmacol. 1996 Jan; 117(1): 203-9. PMID: 8825364, PMCID: PMC1909382, ISSN: 0007-1188.

Singer WD, Brown HA, Bokoch GM, Sternweis PC. Resolved phospholipase D activity is modulated by cytosolic factors other than Arf. J. Biol. Chem. 1995 Jun 6/23/1995; 270(25): 14944-50. PMID: 7797474, ISSN: 0021-9258.

Brown HA, Gutowski S, Kahn RA, Sternweis PC. Partial purification and characterization of Arf-sensitive phospholipase D from porcine brain. J. Biol. Chem. 1995 Jun 6/23/1995; 270(25): 14935-43. PMID: 7797473, ISSN: 0021-9258.

Ktistakis NT, Brown HA, Sternweis PC, Roth MG. Phospholipase D is present on Golgi-enriched membranes and its activation by ADP ribosylation factor is sensitive to brefeldin A. Proc. Natl. Acad. Sci. U.S.A. 1995 May 5/23/1995; 92(11): 4952-6. PMID: 7761430, PMCID: PMC41825, ISSN: 0027-8424.

Brown HA, Sternweis PC. Stimulation of phospholipase D by ADP-ribosylation factor. Meth. Enzymol. 1995; 257: 313-24. PMID: 8583935, ISSN: 0076-6879.

Brown HA, Gutowski S, Moomaw CR, Slaughter C, Sternweis PC. ADP-ribosylation factor, a small GTP-dependent regulatory protein, stimulates phospholipase D activity. Cell. 1993 Dec 12/17/1993; 75(6): 1137-44. PMID: 8261513, PII: 0092-8674(93)90323-I, ISSN: 0092-8674.

Brown HA, Lazarowski ER, Boucher RC, Harden TK. Evidence that UTP and ATP regulate phospholipase C through a common extracellular 5'-nucleotide receptor in human airway epithelial cells. Mol. Pharmacol. 1991 Nov; 40(5): 648-55. PMID: 1944236, ISSN: 0026-895X.

Harden TK, Boyer JL, Brown HA, Cooper CL, Jeffs RA, Martin MW. Biochemical properties of a P2Y-purinergic receptor. Ann. N. Y. Acad. Sci. 1990; 603: 256-66. PMID: 2291525, ISSN: 0077-8923.