Heinrich J. G. Matthies, Ph.D.

Research Assistant Professor

heiner.matthies@vanderbilt.edu
Faculty Appointments
Research Assistant Professor of Molecular Physiology and Biophysics
Education
Ph.D., University of Chicago, Chicago, IllinoisB.A., University of Chicago, Chicago, Illinois
Office Address
702 Light Hall
Nashville, TN 0615
Publications
Bellot M, Galandrin S, Boularan C, Matthies HJ, Despas F, Denis C, Javitch J, Mazères S, Sanni SJ, Pons V, Seguelas MH, Hansen JL, Pathak A, Galli A, Sénard JM, Galés C. Dual agonist occupancy of AT1-R-a2C-AR heterodimers results in atypical Gs-PKA signaling. Nat. Chem. Biol [print-electronic]. 2015 Apr; 11(4): 271-9. PMID: 25706338, PMCID: PMC4465922, PII: nchembio.1766, DOI: 10.1038/nchembio.1766, ISSN: 1552-4469.

Cartier E, Hamilton PJ, Belovich AN, Shekar A, Campbell NG, Saunders C, Andreassen TF, Gether U, Veenstra-Vanderweele J, Sutcliffe JS, Ulery-Reynolds PG, Erreger K, Matthies HJ, Galli A. Rare autism-associated variants implicate syntaxin 1 (STX1 R26Q) phosphorylation and the dopamine transporter (hDAT R51W) in dopamine neurotransmission and behaviors. EBioMedicine. 2015 Feb; 2(2): 135-46. PMID: 25774383, PMCID: PMC4353922, DOI: 10.1016/j.ebiom.2015.01.007, ISSN: 2352-3964.

Hamilton PJ, Shekar A, Belovich AN, Christianson NB, Campbell NG, Sutcliffe JS, Galli A, Matthies HJ, Erreger K. Zn(2+) reverses functional deficits in a de novo dopamine transporter variant associated with autism spectrum disorder. Mol Autism. 2015; 6: 8. PMID: 25741436, PMCID: PMC4349303, PII: 2, DOI: 10.1186/s13229-015-0002-7, ISSN: 2040-2392.

Hamilton PJ, Belovich AN, Khelashvili G, Saunders C, Erreger K, Javitch JA, Sitte HH, Weinstein H, Matthies HJ, Galli A. PIP2 regulates psychostimulant behaviors through its interaction with a membrane protein. Nat. Chem. Biol [print-electronic]. 2014 Jul; 10(7): 582-9. PMID: 24880859, PMCID: PMC4062427, PII: nchembio.1545, DOI: 10.1038/nchembio.1545, ISSN: 1552-4469.

Saunders C, Siuta M, Robertson SD, Davis AR, Sauer J, Matthies HJ, Gresch PJ, Airey DC, Lindsley CW, Schetz JA, Niswender KD, Veenstra-Vanderweele JM, Galli A. Neuronal ablation of p-Akt at Ser473 leads to altered 5-HT1A/2A receptor function. Neurochem. Int [print-electronic]. 2014 Jul; 73: 113-21. PMID: 24090638, PMCID: PMC4073299, PII: S0197-0186(13)00242-8, DOI: 10.1016/j.neuint.2013.09.015, ISSN: 1872-9754.

Bowton E, Saunders C, Reddy IA, Campbell NG, Hamilton PJ, Henry LK, Coon H, Sakrikar D, Veenstra-VanderWeele JM, Blakely RD, Sutcliffe J, Matthies HJ, Erreger K, Galli A. SLC6A3 coding variant Ala559Val found in two autism probands alters dopamine transporter function and trafficking. Transl Psychiatry. 2014; 4: e464. PMID: 25313507, PMCID: PMC4350523, PII: tp201490, DOI: 10.1038/tp.2014.90, ISSN: 2158-3188.

Hamilton PJ, Campbell NG, Sharma S, Erreger K, Hansen FH, Saunders C, Belovich AN, Sahai MA, Cook EH, Gether U, McHaourab HS, Matthies HJ, Sutcliffe JS, Galli A. Drosophila melanogaster: a novel animal model for the behavioral characterization of autism-associated mutations in the dopamine transporter gene. Mol. Psychiatry. 2013 Dec; 18(12): 1235. PMID: 24253181, PII: mp2013157, DOI: 10.1038/mp.2013.157, ISSN: 1476-5578.

Hamilton PJ, Campbell NG, Sharma S, Erreger K, Herborg Hansen F, Saunders C, Belovich AN, , Sahai MA, Cook EH, Gether U, McHaourab HS, Matthies HJ, Sutcliffe JS, Galli A. De novo mutation in the dopamine transporter gene associates dopamine dysfunction with autism spectrum disorder. Mol. Psychiatry [print-electronic]. 2013 Dec; 18(12): 1315-23. PMID: 23979605, PMCID: PMC4046646, PII: mp2013102, DOI: 10.1038/mp.2013.102, ISSN: 1476-5578.

Buchmayer F, Schicker K, Steinkellner T, Geier P, Stübiger G, Hamilton PJ, Jurik A, Stockner T, Yang JW, Montgomery T, Holy M, Hofmaier T, Kudlacek O, Matthies HJ, Ecker GF, Bochkov V, Galli A, Boehm S, Sitte HH. Amphetamine actions at the serotonin transporter rely on the availability of phosphatidylinositol-4,5-bisphosphate. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 Jul 7/9/2013; 110(28): 11642-7. PMID: 23798435, PMCID: PMC3710838, PII: 1220552110, DOI: 10.1073/pnas.1220552110, ISSN: 1091-6490.

Conrad KL, Davis AR, Silberman Y, Sheffler DJ, Shields AD, Saleh SA, Sen N, Matthies HJ, Javitch JA, Lindsley CW, Winder DG. Yohimbine depresses excitatory transmission in BNST and impairs extinction of cocaine place preference through orexin-dependent, norepinephrine-independent processes. Neuropsychopharmacology [print-electronic]. 2012 Sep; 37(10): 2253-66. PMID: 22617356, PMCID: PMC3422490, PII: npp201276, DOI: 10.1038/npp.2012.76, ISSN: 1740-634X.

Hughes SE, Beeler JS, Seat A, Slaughter BD, Unruh JR, Bauerly E, Matthies HJ, Hawley RS. Gamma-tubulin is required for bipolar spindle assembly and for proper kinetochore microtubule attachments during prometaphase I in Drosophila oocytes. PLoS Genet [print-electronic]. 2011 Aug; 7(8): e1002209. PMID: 21852952, PMCID: PMC3154956, PII: PGENETICS-D-11-00796, DOI: 10.1371/journal.pgen.1002209, ISSN: 1553-7404.

Cremona ML, Matthies HJ, Pau K, Bowton E, Speed N, Lute BJ, Anderson M, Sen N, Robertson SD, Vaughan RA, Rothman JE, Galli A, Javitch JA, Yamamoto A. Flotillin-1 is essential for PKC-triggered endocytosis and membrane microdomain localization of DAT. Nat. Neurosci [print-electronic]. 2011 Apr; 14(4): 469-77. PMID: 21399631, PMCID: PMC3066276, PII: nn.2781, DOI: 10.1038/nn.2781, ISSN: 1546-1726.

Speed N, Saunders C, Davis AR, Owens WA, Matthies HJ, Saadat S, Kennedy JP, Vaughan RA, Neve RL, Lindsley CW, Russo SJ, Daws LC, Niswender KD, Galli A. Impaired striatal Akt signaling disrupts dopamine homeostasis and increases feeding. PLoS ONE [print-electronic]. 2011; 6(9): e25169. PMID: 21969871, PMCID: PMC3182178, PII: PONE-D-11-08652, DOI: 10.1371/journal.pone.0025169, ISSN: 1932-6203.

Robertson SD, Matthies HJ, Owens WA, Sathananthan V, Christianson NS, Kennedy JP, Lindsley CW, Daws LC, Galli A. Insulin reveals Akt signaling as a novel regulator of norepinephrine transporter trafficking and norepinephrine homeostasis. J. Neurosci. 2010 Aug 8/25/2010; 30(34): 11305-16. PMID: 20739551, PMCID: PMC3448453, PII: 30/34/11305, DOI: 10.1523/JNEUROSCI.0126-10.2010, ISSN: 1529-2401.

Speed NK, Matthies HJ, Kennedy JP, Vaughan RA, Javitch JA, Russo SJ, Lindsley CW, Niswender K, Galli A. Akt-dependent and isoform-specific regulation of dopamine transporter cell surface expression [letter]. ACS Chem Neurosci [print-electronic]. 2010 Jul 7/21/2010; 1(7): 476-81. PMID: 22778840, PMCID: PMC3368666, DOI: 10.1021/cn100031t, ISSN: 1948-7193.

Matthies HJ, Moore JL, Saunders C, Matthies DS, Lapierre LA, Goldenring JR, Blakely RD, Galli A. Rab11 supports amphetamine-stimulated norepinephrine transporter trafficking. J. Neurosci. 2010 Jun 6/9/2010; 30(23): 7863-77. PMID: 20534835, PMCID: PMC2935280, PII: 30/23/7863, DOI: 10.1523/JNEUROSCI.4574-09.2010, ISSN: 1529-2401.

Bowton E, Saunders C, Erreger K, Sakrikar D, Matthies HJ, Sen N, Jessen T, Colbran RJ, Caron MG, Javitch JA, Blakely RD, Galli A. Dysregulation of dopamine transporters via dopamine D2 autoreceptors triggers anomalous dopamine efflux associated with attention-deficit hyperactivity disorder. J. Neurosci. 2010 Apr 4/28/2010; 30(17): 6048-57. PMID: 20427663, PMCID: PMC2881830, PII: 30/17/6048, DOI: 10.1523/JNEUROSCI.5094-09.2010, ISSN: 1529-2401.

Robertson SD, Matthies HJ, Galli A. A closer look at amphetamine-induced reverse transport and trafficking of the dopamine and norepinephrine transporters. Mol. Neurobiol [print-electronic]. 2009 Apr; 39(2): 73-80. PMID: 19199083, PMCID: PMC2729543, DOI: 10.1007/s12035-009-8053-4, ISSN: 0893-7648.

Steiner JA, Carneiro AM, Wright J, Matthies HJ, Prasad HC, Nicki CK, Dostmann WR, Buchanan CC, Corbin JD, Francis SH, Blakely RD. CGMP-dependent protein kinase Ialpha associates with the antidepressant-sensitive serotonin transporter and dictates rapid modulation of serotonin uptake. Mol Brain. 2009; 2: 26. PMID: 19656393, PMCID: PMC2731736, PII: 1756-6606-2-26, DOI: 10.1186/1756-6606-2-26, ISSN: 1756-6606.

Matthies HJ, Han Q, Shields A, Wright J, Moore JL, Winder DG, Galli A, Blakely RD. Subcellular localization of the antidepressant-sensitive norepinephrine transporter. BMC Neurosci. 2009; 10: 65. PMID: 19545450, PMCID: PMC2716352, PII: 1471-2202-10-65, DOI: 10.1186/1471-2202-10-65, ISSN: 1471-2202.

Mohrmann R, Matthies HJ, Woodruff E, Broadie K. Stoned B mediates sorting of integral synaptic vesicle proteins. Neuroscience [print-electronic]. 2008 Jun 6/2/2008; 153(4): 1048-63. PMID: 18436388, PMCID: PMC2696304, PII: S0306-4522(08)00253-4, DOI: 10.1016/j.neuroscience.2008.02.060, ISSN: 0306-4522.

Cui W, Sproul LR, Gustafson SM, Matthies HJ, Gilbert SP, Hawley RS. Drosophila Nod protein binds preferentially to the plus ends of microtubules and promotes microtubule polymerization in vitro. Mol. Biol. Cell [print-electronic]. 2005 Nov; 16(11): 5400-9. PMID: 16148044, PMCID: PMC1266435, PII: E05-06-0582, DOI: 10.1091/mbc.E05-06-0582, ISSN: 1059-1524.

Huang FD, Matthies HJ, Speese SD, Smith MA, Broadie K. Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction. Nat. Neurosci [print-electronic]. 2004 Oct; 7(10): 1070-8. PMID: 15361878, PII: nn1313, DOI: 10.1038/nn1313, ISSN: 1097-6256.

Rohrbough J, Rushton E, Palanker L, Woodruff E, Matthies HJ, Acharya U, Acharya JK, Broadie K. Ceramidase regulates synaptic vesicle exocytosis and trafficking. J. Neurosci. 2004 Sep 9/8/2004; 24(36): 7789-803. PMID: 15356190, PMCID: PMC2675194, PII: 24/36/7789, DOI: 10.1523/JNEUROSCI.1146-04.2004, ISSN: 1529-2401.

Zhang YQ, Matthies HJ, Mancuso J, Andrews HK, Woodruff E, Friedman D, Broadie K. The Drosophila fragile X-related gene regulates axoneme differentiation during spermatogenesis. Dev. Biol. 2004 Jun 6/15/2004; 270(2): 290-307. PMID: 15183715, PII: S0012160604001265, DOI: 10.1016/j.ydbio.2004.02.010, ISSN: 0012-1606.

Matthies HJ, Broadie K. Techniques to dissect cellular and subcellular function in the Drosophila nervous system. Methods Cell Biol. 2003; 71: 195-265. PMID: 12884693, ISSN: 0091-679X.

Beumer K, Matthies HJ, Bradshaw A, Broadie K. Integrins regulate DLG/FAS2 via a CaM kinase II-dependent pathway to mediate synapse elaboration and stabilization during postembryonic development. Development. 2002 Jul; 129(14): 3381-91. PMID: 12091308, ISSN: 0950-1991.

Matthies HJ, Baskin RJ, Hawley RS. Orphan kinesin NOD lacks motile properties but does possess a microtubule-stimulated ATPase activity. Mol. Biol. Cell. 2001 Dec; 12(12): 4000-12. PMID: 11739796, PMCID: PMC60771, ISSN: 1059-1524.

Zhang YQ, Bailey AM, Matthies HJ, Renden RB, Smith MA, Speese SD, Rubin GM, Broadie K. Drosophila fragile X-related gene regulates the MAP1B homolog Futsch to control synaptic structure and function. Cell. 2001 Nov 11/30/2001; 107(5): 591-603. PMID: 11733059, PII: S0092-8674(01)00589-X, ISSN: 0092-8674.

Matthies HJ, Messina LG, Namba R, Greer KJ, Walker MY, Hawley RS. Mutations in the alpha-tubulin 67C gene specifically impair achiasmate segregation in Drosophila melanogaster. J. Cell Biol. 1999 Dec 12/13/1999; 147(6): 1137-44. PMID: 10601329, PMCID: PMC2168102, ISSN: 0021-9525.

Sekelsky JJ, McKim KS, Messina L, French RL, Hurley WD, Arbel T, Chin GM, Deneen B, Force SJ, Hari KL, Jang JK, Laurençon AC, Madden LD, Matthies HJ, Milliken DB, Page SL, Ring AD, Wayson SM, Zimmerman CC, Hawley RS. Identification of novel Drosophila meiotic genes recovered in a P-element screen. Genetics. 1999 Jun; 152(2): 529-42. PMID: 10353897, PMCID: PMC1460643, ISSN: 0016-6731.

Sharp DJ, McDonald KL, Brown HM, Matthies HJ, Walczak C, Vale RD, Mitchison TJ, Scholey JM. The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles. J. Cell Biol. 1999 Jan 1/11/1999; 144(1): 125-38. PMID: 9885249, PMCID: PMC2148119, ISSN: 0021-9525.

Matthies HJ, McDonald HB, Goldstein LS, Theurkauf WE. Anastral meiotic spindle morphogenesis: role of the non-claret disjunctional kinesin-like protein. J. Cell Biol. 1996 Jul; 134(2): 455-64. PMID: 8707829, PMCID: PMC2120873, ISSN: 0021-9525.

Matthies HJ, Miller RJ, Palfrey HC. Calmodulin binding to and cAMP-dependent phosphorylation of kinesin light chains modulate kinesin ATPase activity. J. Biol. Chem. 1993 May 5/25/1993; 268(15): 11176-87. PMID: 8388385, ISSN: 0021-9258.

Ewald DA, Matthies HJ, Perney TM, Walker MW, Miller RJ. The effect of down regulation of protein kinase C on the inhibitory modulation of dorsal root ganglion neuron Ca2+ currents by neuropeptide Y. J. Neurosci. 1988 Jul; 8(7): 2447-51. PMID: 3249235, ISSN: 0270-6474.

Matthies HJ, Palfrey HC, Miller RJ. Calmodulin- and protein phosphorylation-independent release of catecholamines from PC-12 cells. FEBS Lett. 1988 Mar 3/14/1988; 229(2): 238-42. PMID: 3345844, PII: 0014-5793(88)81132-3, ISSN: 0014-5793.

Matthies HJ, Palfrey HC, Hirning LD, Miller RJ. Down regulation of protein kinase C in neuronal cells: effects on neurotransmitter release. J. Neurosci. 1987 Apr; 7(4): 1198-206. PMID: 3471877, ISSN: 0270-6474.

Lange Y, Matthies HJ. Transfer of cholesterol from its site of synthesis to the plasma membrane. J. Biol. Chem. 1984 Dec 12/10/1984; 259(23): 14624-30. PMID: 6501312, ISSN: 0021-9258.

Lange Y, Matthies H, Steck TL. Cholesterol oxidase susceptibility of the red cell membrane. Biochim. Biophys. Acta. 1984 Feb 2/15/1984; 769(3): 551-62. PMID: 6421320, ISSN: 0006-3002.