Frederick R. Haselton, Ph.D.

Professor

rick.haselton@vanderbilt.edu
Faculty Appointments
Professor of Biomedical Engineering Professor of ChemistryProfessor of Ophthalmology and Visual Sciences
Education
Ph.D., Bioengineering, University of Pennsylvania, Philadelphia, PennsylvaniaB.A., Mathematics, Haverford College, Haverford, Pennsylvania
Office Address
BOX 351510 Station B
Vanderbilt University
Nashville, TN 37235
Research Description
Our intracellular bioengineering approach has resulted in novel experimental and mathematical methods quantifying physiological function at the cellular level and new technologies focused on modifying cell function. We have a primary interest in the role of the endothelial cell and its contributions to both normal maintenance and abnormal dysfunction of vascular physiology.

Current Projects Include:

1. What is the role of the junction proteins in the dynamic regulation of the exchange of proteins and water between the blood and tissues? 2. How can genetic material be most effectively delivered across the cell membrane and to the nucleus? 3. How can the information obtained from microarray hybridization experiments be maximized? 4. Can caging compounds and light be used to locally control the expression of exogenous genetic materials?
Research Keywords
Intracellular Engineering
Publications
McQuain MK, Seale K, Peek J, Fisher TS, Levy S, Stremler MA, Haselton FR. Chaotic mixer improves microarray hybridization. Anal. Biochem. 2004 Feb 2/15/2004; 325(2): 215-26. PMID: 14751256, PII: S000326970300681X, ISSN: 0003-2697.

McQuain MK, Seale K, Peek J, Levy S, Haselton FR. Effects of relative humidity and buffer additives on the contact printing of microarrays by quill pins. Anal. Biochem. 2003 Sep 9/15/2003; 320(2): 281-91. PMID: 12927835, PII: S0003269703003488, ISSN: 0003-2697.

Monroe WT, Haselton FR. Molecular beacon sequence design algorithm. BioTechniques. 2003 Jan; 34(1): 68-70, 72. PMID: 12545542, ISSN: 0736-6205.

McQuain, M.K., Peek, J, Seale, K, Levy, S., Haselton, F.R. Effects of relative humidity and buffer additives on the contact printing of microarrays by quill pins. Anal. Biochem. 2003; 320: 281-91.

Monroe, W.T. and Haselton, F.R. Molecular beacon optimization algorithm. BioTechniques. 2003; 34: 68-73.

Russ PK, Gaylord GM, Haselton FR. Retinal vascular permeability determined by dual-tracer fluorescence angiography. Ann Biomed Eng. 2001 Aug; 29(8): 638-47. PMID: 11556720, ISSN: 0090-6964.

Russ, P.K., Gaylord, G., Haselton, F.R. Retinal vascular permeability determined by dual-tracer fluorescence angiography. Annals of Biomedical Engineering. 2001; 29((3)): 638-47.

Davidson MK, Russ PK, Glick GG, Hoffman LH, Chang MS, Haselton FR. Reduced expression of the adherens junction protein cadherin-5 in a diabetic retina. Am. J. Ophthalmol. 2000 Feb; 129(2): 267-9. PMID: 10682990, PII: S0002939499003232, ISSN: 0002-9394.

Davidson, M.K., Russ,P.K., Radel, C.G., Glick, G.G. and Haselton, F.R. Loss of the adherens junction protein cadherin-5 in a diabetic retina. J. Am. Opthalmology. 2000; 129: 267-9.

Monroe WT, McQuain MM, Chang MS, Alexander JS, Haselton FR. Targeting expression with light using caged DNA. J. Biol. Chem. 1999 Jul 7/23/1999; 274(30): 20895-900. PMID: 10409633, ISSN: 0021-9258.

Tseng WC, Haselton FR, Giorgio TD. Mitosis enhances transgene expression of plasmid delivered by cationic liposomes. Biochim. Biophys. Acta. 1999 Apr 4/14/1999; 1445(1): 53-64. PMID: 10209258, PII: S0167-4781(99)00039-1, ISSN: 0006-3002.

Tseng, W-C, Haselton, F.R. and Giorgio, T.D. Mitosis activates transgene expression of plasmid delivered by cationic liposome. Biochimica Biophysica Acta. 1999; 1445: 53-64.

Monroe, W.T., Chang, M.S. Tseng, W-C, Alexander, J.S. and Haselton, F.R. Induction of caged plasmid expression with light. Journal of Biological Chemistry. 1999; 274((30)): 20895-900.

Russ PK, Davidson MK, Hoffman LH, Haselton FR. Partial characterization of the human retinal endothelial cell tight and adherens junction complexes. Invest. Ophthalmol. Vis. Sci. 1998 Nov; 39(12): 2479-85. PMID: 9804158, ISSN: 0146-0404.

Haselton FR, Dworska EJ, Hoffman LH. Glucose-induced increase in paracellular permeability and disruption of beta-receptor signaling in retinal endothelium. Invest. Ophthalmol. Vis. Sci. 1998 Aug; 39(9): 1676-84. PMID: 9699557, ISSN: 0146-0404.

Alexander JS, Patton WF, Christman BW, Cuiper LL, Haselton FR. Platelet-derived lysophosphatidic acid decreases endothelial permeability in vitro. Am. J. Physiol. 1998 Jan; 274(1 Pt 2): H115-22. PMID: 9458859, ISSN: 0002-9513.

Haselton, F.R. Endothelial Barrier Dynamics: Studies in a Cell-Column Model of the Microvasculature, Chapter in Whole Organ Approaches to Cellular Metabolism. Permeation, Cellular Uptake, and Product Formation. 1998; 135-60.

Haselton, F.R., Dworska, E. and Hoffman, L.H. Glucose increases paracellular permeability and disrupts beta receptor signaling in retinal endothelium. Invest. Ophthalmol. Vis. Sci. 1998; 39: 1676-84.

Alexander, J.S., Robinson, S.A., Chaney, E., Kevil, C.G. and Haselton, F.R. The role of cadherin endocytosis in endothelial barrier regulation: involvement of protein kinase C and actin-cadherin interactions. Inflammation. 1998; 22((4)): 419-33.

Russ, P.K., Davidson, M.K., Hoffman, L.H. and Haselton, F.R. Partial Characterization of the Human Retinal Endothelial Cell Tight and Adherens Junction Complexes. Investigative Ophthalmology & Visual Science. 1998; 39: 2479-85.

Alexander, J.S., Patton, W.F., Christman, B., Larsen, L. and Haselton, F.R. Platelet-derived lysophosphatidic acid decreases endothelial permeability in vitro. Am. J. Physiol. 1998; 274: H115.

Tseng WC, Haselton FR, Giorgio TD. Transfection by cationic liposomes using simultaneous single cell measurements of plasmid delivery and transgene expression. J. Biol. Chem. 1997 Oct 10/10/1997; 272(41): 25641-7. PMID: 9325286, ISSN: 0021-9258.

Haselton FR, Heimark RL. Role of cadherins 5 and 13 in the aortic endothelial barrier. J. Cell. Physiol. 1997 Jun; 171(3): 243-51. PMID: 9180893, PII: 10.1002/(SICI)1097-4652(199706)171:3<243::AID-JCP2>3.0.CO;2-O, DOI: 10.1002/(SICI)1097-4652(199706)171:3<243::AID-JCP2>3.0.CO;2-O, ISSN: 0021-9541.

Haselton FR and Heimark RL. Role of cadherin 5 and cadherin 13 in the aortic endothelial barrier. Journal of Cell Physiol. 1997; 171: 243-51.

Tseng, W., N.B., Haselton, F.R., Giorgio, T.D. Transfection by cationic liposomes using simultaneous measurements of plasmid delivery and transgene expression. J. Biological Chemistry. 1997; 272: 25641-7.

Haselton FR, Woodall JH, Alexander JS. Neutrophil-endothelial interactions in a cell-column model of the microvasculature: effects of fMLP. Microcirculation. 1996 Sep; 3(3): 329-42. PMID: 8930889, ISSN: 1073-9688.

Haselton FR, Dworska E, Evans SS, Hoffman LH, Alexander JS. Modulation of retinal endothelial barrier in an in vitro model of the retinal microvasculature. Exp. Eye Res. 1996 Aug; 63(2): 211-22. PMID: 8983979, PII: S0014483596901104, ISSN: 0014-4835.

Haselton, F.R., Alexander, J.S. and Woodall, J.H. Neutrophil-endothelial interactions in a cell-column model of the microvasculature: effects of fMLP. Microcirculation. 1996; 3((3)): 329-42.

Tseng, W., Purvis, N.B., Haselton, F.R., Giorgio, T.D. Cationic liposomal delivery of plasmid to endothelial cells measured by quantitative flow cytometry. Biotechnology and Bioengineering. 1996; 50: 548-54.

Haselton, F.R., Alexander, J.S., Dworska, E, Evans, S.S. and Hoffman, L.H. Modulation of retinal endothelial barrier in an in vitro model of the retinal microvasculature. Exp. Eye Res. 1996; 63: 211-22.

Harris TR, Waters CM, Haselton FR. Use of scaling theory to relate measurements of lung endothelial barrier permeability. J. Appl. Physiol. 1994 Nov; 77(5): 2496-505. PMID: 7868472, ISSN: 8750-7587.

Harris, T.R., Waters, C.M. and Haselton, F.R. The use of scaling theory to relate measures of lung endothelial barrier permeability. J. Appl. Physiol. 1994; ((5)): 2496-505.

Alexander JS, Blaschuk OW, Haselton FR. An N-cadherin-like protein contributes to solute barrier maintenance in cultured endothelium. J. Cell. Physiol. 1993 Sep; 156(3): 610-8. PMID: 8360263, DOI: 10.1002/jcp.1041560321, ISSN: 0021-9541.

Waters CM, Alexander JS, Harris TR, Haselton FR. Perilla ketone increases endothelial cell monolayer permeability in vitro. J. Appl. Physiol. 1993 May; 74(5): 2493-501. PMID: 7687599, ISSN: 8750-7587.

Haselton FR, Alexander JS, Mueller SN. Adenosine decreases permeability of in vitro endothelial monolayers. J. Appl. Physiol. 1993 Apr; 74(4): 1581-90. PMID: 7685754, ISSN: 8750-7587.

Haselton, F.R., Alexander, J.S., Mueller, S.N. Adenosine decreases permeability of endothelial monolayers. J. Appl. Physiol. 1993; 74((4)): 1581-90.

Waters, C.M., Alexander, J.S., Harris, T.R., Haselton, F.R. Perilla ketone increases endothelial cell monolayer permeability in vitro. J. Appl. Physiol. 1993; 74((5)): 2493-501.

Alexander, J.S., Blaschuk, O., Haselton, F.R. An N-cadherin-like protein contributes to solute barrier maintenance in cultured endothelium. J. Cell. Physiol. 1993; 156: 610-8.

Haselton FR, Alexander JS. Platelets and a platelet-released factor enhance endothelial barrier. Am. J. Physiol. 1992 Dec; 263(6 Pt 1): L670-8. PMID: 1476205, ISSN: 0002-9513.

Haselton, F.R., Alexander, S.A., Mueller, S.N., Fishman, A.P. Modulation of endothelial paracellular permeability: A mechanistic approach. Endothelial Cell Dysfunctions, pp. , N. & M. Simionescu, editors, Plenum, New York. 1992; 103-26.

Haselton, F.R., Alexander, J.S. Platelets and platelet releasate enhance endothelial barrier. Am. J. Physiol. 1992; 263: L670-L678.

Howell, R.E., Haselton, F.R., Mueller, S.N. Angiotensin-converting enzyme kinetics in an endothelial cell column. Am. J. Physiol. 1990; 258: L188-L194.

Haselton, F.R., Mueller, S.N., Howell, R.E., Levine, E.M., Fishman, A.P. Chromatographic demonstration of reversible changes in endothelial permeability. J. Appl. Physiol. 1989; 67((5)): 2032-48.

Scherer, P.W., Muller, W.J., Raub, J.B., Haselton, F.R. Convective mixing mechanisms in high frequency jet ventilation. Acta. Anaesthesiol. Scand. 1989; 90: 58-64.

Howell, R.E., Hansen-Flaschen, J.H., Haselton, F.R., Lanken, P.N., Fishman, A.P. Pulmonary extraction of propranolol in normal and oxygen-toxic sheep. J. Pharmacol. & Exp. Thera. 1989; 250((1)): 93-9.

Albelda, S.M., Sampson, P.M., Haselton, F.R., McNiff, J.M., Mueller, S.N., Williams, S.K., Fishman, A.P., Levine, E.M. The permeability characteristics of cultured endothelial cell monolayers. J. Appl. Physiol. 1988; 64: 308-22.

Haselton, F.R., Sperandio, P.G.N. Convective exchange between the nose and the atmosphere. J. Appl. Physiol. 1988; ((64)): 2575-81.

Haselton, F.R., Mueller, S.N., Howell, R.E. Analysis of chromatography data from an endothelial cell column. Proceedings of 13th N.E. Bioengineering Conference, Philadelphia, PA. 1987; 12-3.

Parker, R.E., Roselli, R.J., Haselton, F.R., Harris, T.R. Effect of perfusate hematocrit on urea permeability-surface area in isolated dog lung. J. Appl. Physiol. 1986; 61((4)): 1383-7.

Lanken, P.N., Hansen-Flaschen, J.H., Sampson, P.M., Pietra, G.G., Haselton, F.R., Fishman, A.P. The passage of uncharged dextrans from blood to lung lymph in awake sheep. J. Appl. Physiol. 1985; 59((2)): 580-91.

Haselton, F.R., Parker, R.E., Roselli, R.J., Harris, T.R. Lung multiple tracer analysis with an effective-diffusivity model of capillary tissue exchange. J. Appl. Physiol. 1984; 57((1)): 98-109.

Scherer, P.W., Haselton, F.R., Seyburt, J.R. Gas transport in branched airways during high frequency ventilation. Ann. Bioengr. 1984; 12: 385-405.

Haselton, F.R., Roselli, R.J., Parker, R.E., Harris, T.R. Mathematical models of pulmonary capillary-tissue exchange: limiting vases, sensitivity analysis and moment comparison. Mathematical Biosciences. 1984; 70: 237-63.

Haselton, F.R., Parker, R., Roselli, R.J., Harris, T.R. Comparison of three models of capillary tissue exchange in isolated perfused dog lungs. Microvasc. Res. 1983; 3: 237.

Haselton, F.R., Scherer, P.W. Flow visualization of steady streaming in oscillatory flow through a bifurcating tube. J. Fluid Mech. 1982; 123: 315-33.

Scherer, P.W., Haselton, F.R. Convective exchange in oscillatory flow through the bronchial tree. J. Appl. Physiol. 1982; 53((4)): 1023-33.

Haselton, F.R., Scherer, P.W. Bronchial bifurcations and respiratory mass transport. Science. 1980; 208((4)): 69-71.

Scherer, P.W., Haselton, F.R. A network theory of bronchial gas mixing applied to single breath nitrogen washout. Lung. 1980; ((158)): 201-20.

Scherer, P.W., Haselton, F.R. Convective mixing in tube networks. AIChE J. 1979; 3((25)): 542-4.

Scherer, P.W., Haselton, F.R., Hanna, L., Stone, D. Growth of hygroscopic aerosols in a model of bronchial airways. J. Appl. Physiol. 1979; 3((47)): 544-50.

Scherer, P.W., Haselton, F.R. Deposition of hygroscopic aerosols in a five generation model of the bronchial tree. Am. Rev. Respir. Dis. 1978; 4((117)): 258.

Balasubramanian, B. Bhuva, R. Mernaugh, and F.R. Haselton. Si-based sensor for virus detection. IEEE.